Foods
·
2025
·Vol. 14
·期 13
·2277
·
IF 5.1
Haoxuan Zhang,Huange Zhang,Hao Zhong
Lactobacillus plantarumexhibits probiotic effects, including regulating the balance of the intestinal microbiota and enhancing immune function. However, this strain often experiences viability loss upon ingestion due to harsh conditions within the human digestive tract. This study aimed to evaluate the efficacy of metal–phenol networks (MPNs) fabricated via three polyphenols—tannic acid (TA), tea polyphenol (TP), and anthocyanin (ACN)—combined with Fe(III) coatings in protectingLactobacillus plantarumduring simulated digestion and storage. The results demonstrated that MPNs formed a protective film on the bacterial surface. While TA and ACN inhibited the growth ofLactobacillus plantarumYJ7, TP stimulated proliferation. Within the MPNs system, only Fe(III)-TA exhibited growth-inhibitory effects. Notably, ACN displayed the highest proliferation rate during the initial 2 h, followed by TP between 3 and 4 h. All MPN-coated groups maintained high bacterial viability at 25 °C and −20 °C, with TP-coated bacteria showing the highest viable cell count, followed by TA and ACN. In vitro digestion experiments further revealed that the Fe(III)-ACN group exhibited the strongest resistance to artificial gastric juice. In conclusion, tea polyphenol and anthocyanin demonstrate superior potential for probiotic encapsulation, offering both protective stability during digestion and enhanced viability under storage conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD QUALITY
·
2025
·Vol. 2025
·期 1
·5049955
·
IF 2.9
Jiyou Wu,Xiaoguang Yang,Yuhua Yang
This study aims to investigate the potential anti-inflammatory and antioxidant properties of Lactobacillus brevis CQPC12 (LBCQPC12) to enhance brain, neural, and motor functioning in mice. Mice were given intraperitoneal injections of mixed antibiotics (5 mg/mL neomycin, 25 mg/mL vancomycin, 0.1 mg/mL amphotericin B, 10 mg/mL ampicillin, 5 mg/mL metronidazole, and 1.5 μg/mL lipopolysaccharide) to mimic human bodies. At the conclusion of the trial, the mice’s capacity to run for prolonged periods of time and swim with weight was assessed. Furthermore, hematoxylin–eosin (H&E) staining was employed to investigate pathological alterations in brain tissue; quantitative polymerase chain reaction (qPCR) was applied to discover inflammatory pathway genes and vascular endothelial growth factor (VEGF)-related expressions in the brain and skeletal muscle, and the liver index, serum, and brain oxidation, and inflammation indicators were found. The findings demonstrated that LBCQPC12 increased total superoxide dismutase (T-SOD), glutathione (GSH), and interleukin-10 (IL-10) levels while decreasing malondialdehyde (MDA), IL-6, and tumor necrosis factor alpha (TNF-α) levels in the serum and brain, as well as lengthening the mice’s weight-carrying swimming and running durations and lowering the liver index. LBCQPC12 boosted the expression of VEGF-A, glucose transporter 1 (GLUT-1), GLUT-4, and hypoxia-inducible factor-1 alpha (HIF-1α) mRNA in skeletal muscle as well as genes in the brain’s protein kinase B 1/cAMP-response element binding protein/brain-derived neurotrophic factor/extracellular regulated protein kinases 1 (AKT1/CREB/BDNF/ERK1) pathways. Finally, LBCQPC12 demonstrated potential utility in the creation of functional probiotics by reducing oxidative inflammation, skeletal muscle damage, and motor function in the central nervous system brought on by antibiotics.
研究领域:
微生物学
·
神经科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Fei He,Xue-Ke Ma,Cheng-Kai Tu,Hui Teng,Xin Shao,Jie Chen,Meng-Xin Hu
Supplementing probiotics or indigestible carbohydrates is a usual strategy to prevent or revert unhealthy states of the gut by reshaping gut microbiota. One criterion that probiotics are efficacious is the capacity to survive in the gastrointestinal tract. Biofilm is the common growth mode of microorganisms with high tolerances toward harsh environments. Suitable scaffolds are crucial for successful biofilm culture and large-scale production of biofilm-phenotype probiotics. However, the role of scaffolds containing indigestible carbohydrates in biofilm formation has not been studied. In this study, porous zein/cellulose composite scaffolds provided nitrogen sources and carbon sources simultaneously at the solid/liquid interfaces, being beneficial to the biofilm formation of Lactobacillus reuteri . The biofilms showed 2.1–17.4 times higher tolerances in different gastrointestinal conditions. In human fecal fermentation, the biofilms combined with the zein/cellulose composite scaffolds act as the “synbiotics” positively modulating the gut microbiota and the short-chain fatty acids (SCFAs), where biofilms provide probiotics and scaffolds provide prebiotics. The “synbiotics” show a more positive regulation ability than planktonic L. reuteri , presenting potential applications in gut health interventions. These results provide an understanding of the synergistic effects of biofilm-phenotype probiotics and indigestible carbohydrates contained in the “synbiotics” in gut microbiota modulation.
研究领域:
微生物学
·
食品科学
·
营养学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shu-Min Hu,Jia-Min Zhou,Qing-Qing Zhou,Ping Li,Yuan-Yuan Xie,Tao Zhou,Qing Gu
Exopolysaccharides (EPS) produced by Lactobacillus rhamnosus ZFM231 isolated from milk were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence, obtaining four purified fractions EPS-1, EPS-2, EPS-3 and EPS-4. Among them, EPS-1 was a neutral polysaccharide and the others were acidic polysaccharides. The four factions were characterized by chemical composition analysis, molecular weight measurement, and UV, IR, 1 H and 13 C NMR spectra analysis. The antioxidant activity assays indicated that the purified fractions possessed appreciable ferric iron reducing power, free radical (DPPH, hydroxyl and superoxide anion radicals) scavenging ability and ferrous iron chelating ability. These purified exopolysaccharides were also found to possess marked hypolipidemic activity in vitro . The results showed that they were able to effectively inhibit lipase activity, and possessed remarkable activity in reducing cholesterol and triglyceride levels in the HepG2 cell models. Therefore, Lactobacillus rhamnosus ZFM231 and EPS produced by this probiotic strain may find application in food and pharmaceutical industries.
研究领域:
食品科学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 19
·7983-7995
·
IF 3.67
Guo Jingui,Xie Yixiao,Yu Zhu,Meng Geng,Wu Zhe
For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The supernatant of L. plantarum expressing CbXyn10C and Bgxg1 (LP11AG) showed activities on xylan, Avicel, and carboxymethylcellulose (CMC), while the supernatant of the wild-type L. plantarum showed no activity. When LP11AG was used as silage additive, the water-soluble carbohydrate content of alfalfa silage increased by 72%, 55%, and 155% compared with control when the silage was stored at 20 °C, 30 °C, and 40 °C, respectively. With LP11AG being used as an additive for the alfalfa silage stored at 20 °C, the hemicellulose, cellulose, and acid detergent ligninin (ADL) contents decreased by 17%, 6%, and 14% compared with the control ( p < 0.05), respectively. Compared with the corresponding original contents, the contents of glucose, arabinose, galactose, and fructose detected in silage treated with LP11AG after 45 days of ensiling increased by 55%, 1494%, 68%, and 5% , respectively, when stored at 40 °C. Raffinose and stachyose, originally present in alfalfa, disappeared after ensiling. In conclusion, our results suggest that LP11AG provides a substantial benefit as a silage additive.
研究领域:
微生物学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Mingguang Yu,Baosong Wang,Ying Wang,Yuan Tang,Chen Liu,Huanlu Song,Baochao Hou,Baolei Li,Wen Zhao
Odor is an important attribute of fermented dairy products and is an important factor in determining consumer acceptance. However, the odor characteristics of brown lactobacillus beverage (BLB) and the differences in odor among different types of these products have rarely been studied. Here, a comprehensive analysis of different types of BLB were performed by SPME–GC–O–MS and SPME–GC×GC–O–MS techniques based on untargeted metabolomics. Compared with SPME–GC–O–MS, SPME–GC×GC–O–MS identified 26 new odor compounds in BLB, indicating that this method is more suitable for odor characterization of BLB. A total of 149 odor compounds were identified from 13 BLBs, of which 40 were odor-active compounds. Ketones , alcohols, acids, aldehydes, and esters accounted for about 80% of the total odor compounds and constituted the basic odor profile of the BLB. In addition to identifying indicator flavor compounds such as furfuryl alcohol , octanoic acid , and β -myrcene, which are responsible for the differences in odor profiles between different BLBs, the reference content ranges in BLBs were precisely quantified. These findings will provide new insights into the species traceability and odor characterization of BLB samples.
研究领域:
食品科学
·
分析化学
·
微生物学
·
代谢组学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2016
·
IF 3.858
Meng Zhao,Fangning Qu,Zhengjun Wu,Katsuyoshi Nishinari,Glyn O. Phillips,Yapeng Fang
Alginate microcapsule is widely used to encapsulate and protect lactic acid bacteria (LAB). This study aims to design microcapsules with different mechanical strength and clarify their protecting mechanism for Lactobacillus plantarum ST-III under simulated digestive conditions through physical and physiological analysis. By controlling viscosity and gelling ability of alginate solution, a series of microcapsules with the same particle size (ca. 400 μm) and varying mechanical strength (5.4–51.9 N) were prepared. Cell survivals in simulated gastric juice (SGJ) and bile salts (BS) were found to be positively correlated with the mechanical strength of microcapsules. Diffusion experiments using different probes showed that the permeability coefficients of the microcapsules decreased with increasing mechanical strength. Moreover, membrane integrity and membrane fluidity of encapsulated LAB in SGJ were reduced to a less extent and tended to maintain at normal physiological values, with increasing mechanical strength. In contrast, cell membrane integrity was much more reduced (<4.0%) and membrane fluidity was markedly augmented in BS, regardless of the mechanical strength of microcapsules, indicating seriously damaged cells with abnormal physiological properties. These physical and physiological indexes together manifested the protection mechanism of alginate microcapsules with different mechanical strength for LAB.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
·
2017
·
IF 1.059
Sheng Hu,Xiang Zhang,Yi Lu,Yue-Cheng Lin,Dong-Fang Xie,Hui Fang,Jun Huang,Le-He Mei
An aspartate aminotransferase (AATase) gene from Lactobacillus brevis CGMCC 1306 was cloned, which contains a 1182-bp open reading frame coding for 393 amino acids (41.43 kDa). When expressed in Escherichia coli BL21 (DE3), the recombinant AATase was purified and subsequently characterized. The recombinant AATase can catalyse the conversion of L-Asp to L-Glu, and the kcat/Km was determined to be 25.5 (mmol/L)−1 s−1 for L-Asp and 207.8 m(mol/L)−1 s−1 for α-ketoglutarate. With optimum temperature as 25 ˚C, the AATase may be a novel and special psychrophilic enzyme which exhibited a good thermal stability below 55 ˚C. The conserved active site residue of AATase was identified as Lys237 by phylogenetic analysis. Secondary structure of the enzyme includes α-helix (39.2%), β-sheet (5.5%), β-turn (8.8%), and random coil (36.5%) by circular dichroism spectral analysis. Phase diagram for the fluorescence data analysis showed that guanidinium chloride-induced unfolding of AATase involved at least one intermediate.
研究领域:
微生物学
·
生物化学
·
酶学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2018
·
IF 3.47
Yaser Gamallat,Xiaomeng Ren,Williams Walana,Abdo Meyiah,Ren Xinxiu,Yanyan Zhu,Meiqi Li,Siyuan Song,Luhan Xie,Yazeed Jamalat,Mohammad Zubair Saleem,Yufang Ma,Yi Xin,Dong Shang
Lactobacillus rhamnosus (LGG) is well documented strain with wide clinical applications. We investigated herein the possible protection role of LGG in against early preneoplastic colon carcinogenesis Aberrant crypt foci (ACF) in rat model. Sprague Dawley rats were pre-treated with or without LGG via oral gavage, followed by ACF induction with Dimethylhydrazine (DMH). LGG treated groups showed significant decrease in total number of ACF’s, and increased Bax, P53, and Caspase-3 proteins expression with significant attenuation of NFkB, Cox2 and β catenin expression compared to DMH group. Serum cytokines array revealed significant elevation of IL-2, IL-6, and IFN-γ, and attenuated level of IL-10 in LGG+DMH treated animals. Finally, the 16S rRNA fecal microbiome genomic analysis showed that LGG alters the gut microbiome structure, composition and functions at phylum, family and genus level. Collectively, LGG reduces colon ACFs incidence and multiplicity via inhibition of pro-carcinogenesis biomarkers and modulate the gut microbiome.
研究领域:
胃肠病学
·
微生物学
·
肿瘤学
·
分子生物学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Xue Bai,Mengzhen Han,Tianli Yue,Zhenpeng Gao
To control the post-acidification caused by the continued fermentation of Lactobacillus in fermented apple juice (FAJ) during cold storage and establish a shelf-life prediction model of the post-acidification process, the effects of nisin and ε-polylysine on the physicochemical and microbiological properties of FAJ during cold storage were evaluated. In addition, quality indexes and shelf-life prediction models of fermented apple juice with the addition of nisin (FAJN) were established and verified. The post-acidification control effect of 40 mg/L nisin was determined to be optimal. Furthermore, deterioration of quality indicators of FAJ treated with 40 mg/L nisin was significantly controlled, and the shelf-life was prolonged compared with FAJ. Prediction models of L*, a*, b*, ΔE, viable cell count, and the stability coefficient of FAJN were established and verified, and a shelf-life prediction model was established with the number of viable bacteria as the prediction indicator. Relative errors between the predicted and measured values of each model were within ±10%, indicating that the models could predict changes in each index and the shelf-life of FAJN when stored at 4 °C and 25 °C.
研究领域:
食品科学
·
微生物学
·
食品技术
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Dai Junjie,He Jiayi,Chen Zixi,Qin Huan,Du Ming,Lei Anping,Zhao Liqing,Wang Jiangxin
Euglena gracilis, a single-celled microalga with various trophic growth styles under different cultivation conditions, contains nutrients, such as ß-1,3-glucans, essential amino acids, fatty acids, vitamins, and minerals. It has recently attracted attention as a new health food. Among them, ß-1,3-glucans, paramylon of Euglena, is an insoluble dietary fiber and is well known as an immune booster, attenuator of obesity and diabetes, reducer of acute liver injury, and suppressor of atopic dermatitis, and other chronic inflammatory disorders. Recently, evidence has appeared for the positive health effects of foods, food ingredients, or biochemical compounds derived from several other microalgae, such as Chlorella, Spirulina, Dunaliella, Phaeodactylum, and Pavlova. Until most recently, the prebiotic activity of Euglena and paramylon was reported. Emerging prospects of microalgae as prebiotics were well summarized, but the mechanisms behind the bacterial growth promotion by microalgae are not elucidated yet. Thus, we evaluated the prebiotic prospects of both autotrophic and heterotrophic Euglena on six different Lactobacillus. What’s more, the stimulated mechanism was revealed by bacterial culture medium metabolomic analysis. This study could widen the knowledge about the prebiotic activity of Euglena as a next-generation prebiotic and other microalgae-derived compounds as potential health foods.
研究领域:
微生物学
·
营养学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qi Guo,Jiaxin Tang,Shidong Li,Liyue Qiang,Shuaidan Chang,Gengan Du,Tianli Yue,Yahong Yuan
Probiotics incorporated into food products are often insufficiently potent because of unfavorable interference during processing. This study addressed these issues by encapsulating Lactobacillus plantarum into whey protein isolate (WPI) and dextran (DX) conjugates using an encapsulator. The objective of this work is to investigate the effect of encapsulation using WPI and DX conjugates on the viability of Lactobacillus plantarum . The formation of the conjugates was confirmed with absorbance of the intermediate- and late-stage products, degree of grafting, browning index, and gel electrophoresis. Extending the heating time from 1 to 5 h increased the content of high molecular weight conjugates. These conjugates provided better protection of probiotics under processing stress conditions, gastrointestinal conditions, and storage. Furthermore, encapsulated L. plantarum exhibited a reduction of only 0.33 log CFU/mL after 90 d of storage at 4 °C. Thus, microencapsulation of probiotics can enhance the viability of functional food products and facilitate corresponding research.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）