Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-17
·
IF 5.2
Sun Zhiqiang,Li Yuan,Li Shuangye,Wang Siyuan,Li Siyi,Ke Yan,Gao Run,Wang Lei,Zhou Zhenming,Wu Zhe,Yu Zhu
Background Sweet sorghum ( Sorghum bicolo r (L.) Moench) is a popular forage crop in arid and semi-arid areas due to its high drought tolerance, rapid growth, and low production costs. In addition, sweet sorghum, has relatively specific ensiling characteristics because of its high moisture content and sufficient amount of water soluble carbohydrates. Therefore, it is crucial to investigate the raw material characteristics and exogenous additive pretreatment for the regulation of silage quality. This study aimed to evaluate the effects of Lactobacillus plantarum (LP) and cellulase (CEL) on the fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities of sweet sorghum silages with two different raw material characteristics (fresh sweet sorghum material (MC1); sweet sorghum material wilted outdoors for 6 h after chopping (MC2)). Results In this study, the sweet sorghum treatments were: control (without additives), LP, CEL, or a combination of LP and CEL (LP_CEL). All treated sweet sorghum samples were ensiled for 30 d. A higher content of lactic acid, propionic acid, D-fructose, glucose, sucrose and a lower content of structural carbohydrates were observed in MC1 silage than in MC2 silage. In MC2 silage, the addition of CEL or LP_CEL decreased the content of structural carbohydrates, while it increased the content of D-fructose, glucose, D-arabinose, xylitol, maltose and trehalose ( P < 0.05). The in vitro gas production at 48 h was greater in MC1 silage than in MC2 silage, and the addition of CEL or LP_CEL increased the in vitro dry matter digestibility in MC2 silage ( P < 0.05). After 30 d of ensiling, disaccharides such as sucrose, maltose and alginate were almost entirely utilized by the microorganisms, while more-consumed monosaccharides included D-fructose, glucose and L-rhamnose. Lactobacillus was the dominant genus (> 80% relative abundance) in all silage samples. Conclusions Raw material characteristics determine carbohydrate composition, in vitro digestibility, and microbial community of sweet sorghum silage. For wilted sweet sorghum with relatively low moisture content, pretreatment with CEL or LP_CEL reduced the structural carbohydrate content, increased the nonstructural carbohydrate content, and improved the digestibility of the silage. However, additives had no obvious impact on enhancing the fermentation quality of sweet sorghum silage for two raw material characteristics. Graphical abstract
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yaowen Liu,Yue Wang,Siwei Shen,Md Alomgir Hossen,Dur E. Sameen,Saeed Ahmed,Jianwu Dai,Siyu Xiao,Suqing Li,Wen Qin
In order to increase the stability and the release time of nisin, nisin/ Tremella fuciformis polysaccharide nanoparticles (NTNs) were prepared and evaluated based on their uniform particle size dispersion (168–356 nm) and high loading rate (70–86%). The condensation reaction between 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and hydroxysuccinimide (EDC/NHS) amide was successfully used to prepare novel live particles (NTN@LP). When the mass ratio of NTN and LP was 2:1, the bacterial viability (85.6 ± 2.11%) and grafting efficiency (68.8 ± 1.96%) of the NTN@LP were both relatively high. The hemolytic activity and in vitro cytotoxicity indicated that NTN@LP is safe to use in food. The NTN@LPs were then used as natural microbial preservatives in sausages. The pH value, water activity, thiobarbituric acid-reactive substances value, and sensory evaluation of the sausages were used to assess the effect of NTN@LPs on the quality of the sausages during fermentation. NTN@LPs played an important role in reducing and decomposing biogenic amines (BAs) throughout the sausage fermentation process and displayed a strong inhibitory effect on histamine (>95%). Compared to that of the control group, the total amount of BAs in the 2:1 group was reduced by 67.90%. Tyramine content in the 2:1 NTN@LP group decreased by 78.16% compared to that in the control group, while tyramine content in the pure LP group was decreased by only 38.25%. These results indicate that NTN@LP can be potentially applied as a natural microbial preservative in the food industry.
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高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Xinran Lv,Huanhuan Ma,Mengtong Sun,Yang Lin,Fengling Bai,Jianrong Li,Bolin Zhang
A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish ( Trichiurus lepturus ) and identified as Lactobacillus plantarum . This bacteriocin reached the maximum production at stationary phase after 24 h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30 min at 121 °C) and kept active at the pH range of 2.5–5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens , P. aeruginosa , Vibrio parahaemolyticus , Aeromonas sobria and Listeria monocytogenes . The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4 °C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative.
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Wan Wang,Cong Xu,Xuan Zhou,Le Zhang,Liya Gu,Zhijing Liu,Jiage Ma,Juncai Hou,Zhanmei Jiang
Probiotics and prebiotics have received attention in alleviating neurodegenerative diseases. Lactobacillus plantarum (L. plantarum) 69-2 was combined with galactooligosaccharides (GOS) and supplemented in a d-galactose (d-gal)-induced neurodegeneration and memory impairment mice model to explore its effects on the brain and the regulation of short-chain fatty acids. The results showed that the L. plantarum-GOS supplementation inhibited d-gal-induced oxidative stress and increased the brain’s nuclear factor erythroid 2-related factor 2 (Nrf2) levels. Butyrate, a metabolite of the gut microbiota regulated by L. plantarum combined with GOS, inhibits p-JNK expression, downregulates pro-apoptotic proteins expression and the activation of inflammatory mediators, and upregulates synaptic protein expression. This might be a potential mechanism for L. plantarum 69-2 combined with GOS supplementation to alleviate d-gal-induced neurodegeneration and memory impairment. This study sheds new light on the development of aging-related neuroprotective dietary supplements based on the gut–brain axis.
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Zhe Zhao,Guijie Chen,Danyang Li,Qi Zhang,Guoyuan Xiong,Chuanlai Du,Xiaoxiong Zeng,Chunxu Chen
The 3,5-dicaffeoylquinic acid (3,5-DiCQA), a key component of Chuzhou chrysanthemum, one of the “Four Major Edible Chrysanthemums” in China, has been shown to enrich beneficial gut bacteria and reduce lipid synthesis based on its hydroxycinnamic acid structures. However, its low lipophilicity limits bioactivity, necessitating the development of more stable derivatives. Therefore, this study synthesized and characterized a novel esterified derivative 3,5-DiCQA dodecyl ester (3,5-DiCQAD) with markedly enhanced lipophilicity and bioactivity. In high-fat diet (HFD)-induced male C57BL/6J mice, 3,5-DiCQAD showed remarkable metabolic regulatory effects, including improvements in glucose metabolism, lipid homeostasis, BA signaling, and thermogenesis. Furthermore, 3,5-DiCQAD effectively reshaped the gut microbiota, enriching beneficial bacteria such as Lactobacillus plantarum . Moreover, the strain L. plantarum ZZ 240321 was isolated, and its fermentation of 3,5-DiCQAD generated metabolites that modulated bile acid (BA) metabolism through the FXR/SHP/LRH-1 pathway, alleviating obesity-related symptoms in antibiotic-treated mice. This research provides a novel perspective for developing plant-derived polyphenols as anti-obesity treatments. It also broadens the understanding of DiCQA's probiotic potential in the gut microbiome, highlighting its promising role in managing metabolism-associated disorders.
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高影响因子
危险品化学品经营许可证（带存储）