SENSORS AND ACTUATORS B-CHEMICAL
·
2023
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IF 8.4
Lanmei Gao,Houbing Zheng,Yuanlong Hu,Yi Zhong,Linhai Jiang,Yuanzi Wu,Fen Yan,Da Huang,Jianhua Li,Fang Zhang,Zhenyu Lin,Meishui Wang,Zuquan Weng
Bacterial infection of wounds is one of great concern to patients, and rapid and correct detection of bacterial infection is crucial to ensure accurate diagnosis and early intervention. Based on the principle that glucose can only be consumed by live bacteria, a fluorescent biosensor was constructed to detect four kinds of common infectious bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in skin wounds, taking advantage of magnetic bead-aptamer for recognizing, sorting and enrichment, platinum nanoparticles for signal amplification . The linear detection range of AB, EC, PA and SA were 27– 8 × 10 6 CFU/mL, 10– 2.5 × 10 7 CFU/mL, 34– 2.5 × 10 7 CFU/mL and 10– 1.0 × 10 7 CFU/mL, respectively, and the limits of detection were 27 CFU/mL, 10 CFU/mL, 34 CFU/mL and 2 CFU/mL. Furthermore, all target bacteria in samples containing 8 × 10 8 CFU/mL of other interfering bacteria have been successfully identified and quantified. The proposed method was also successfully applied to the detection of bacterial infection in skin wounds of mouse and human, including the detection of separate bacterial infection as well as a coinfection. The recovery of this method was in the range of 90.161– 109.961%. Thus, this proposed method can be a promising candidate for rapid and convenient evaluation of infectious bacteria in point-of-care settings.
研究领域:
医学诊断
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生物传感技术
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纳米医学
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产品关联
DOI
高影响因子
Results in Chemistry
·
2025
·
IF 4.2
Basharat Ali,Muhammad Riaz,Ajmal Khan,Nadir Rasheed,Muhammad Masood,Ali Iftikhar,Amir Faisal,Sobia Ahsan Halim,Jalal Uddin,Afnan Jan,Ahmed Al-Harrasi
Carbothioamides exhibit many biological properties, including antiviral, antibacterial and anticancer activities. Their ability to interfere with DNA synthesis is mainly responsible for their anticancer activities. Here, twelve phenyl-substituted carbothioamides ( 2−13 ) were synthesized and characterized using Electron Ionization Mass Spectrometry (EI-MS), proton and carbon-13 nuclear magnetic resonance ((Singhal et al., 2013 [ 1 ] ) HNMR, and 13 C NMR), Infrared (IR), and elemental analysis. The anticancer activity of the carbothioamide derivatives ( 2–13 ) was measured in the HCT116 colorectal cancer cell line by means of a three day sulforhodamine B (SRB) proliferation assay. Among the series, four compounds ( 2 , 4 , 11 , and 12 ) which inhibited the cell viability by more than 50 % at 50 μM concentration. IC 50 determination of these four compounds revealed 1-benzoyl-4-(2-bromophenyl) thiosemicarbazide ( 11 ) as the most potent compound with IC 50 values of 21.61 ± 4.77 μM, 18.34 ± 3.53 μM and 17.87 ± 12.02 μM in the breast cancer (MCF-7) cell line, human colorectal carcinoma (HCT116) cell line and MD Anderson-metastatic breast (MDA-MB-453) cell lines respectively. Prolonged treatment of cells with the compound resulted in apoptotic cell death as determined by cleavage of Poly (ADP-ribose) polymerase (PARP), a caspase 3 substrate and an apoptotic marker. This study, therefore, explored novel derivatives of carbothioamides for their anticancer activities; the structure activity relationship (SAR) reported in this study and predicted docking analysis and ADMET profile will help optimize carbothioamides as anticancer agents.
研究领域:
药物化学
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药理学
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肿瘤学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
Theranostics
·
2017
·
IF 8.766
Ying Sun,Qi Wang,Jianhua Chen,Lei Liu,Li Ding,Ming Shen,Jin Li,Baoshan Han,Yourong Duan
Gold nanoparticle-coated Pluronic- b -poly(L-lysine) nanoparticles (Pluronic-PLL@Au NPs) were synthesized via an easy one-step method and employed as carriers for the delivery of paclitaxel (PTX) in chemo-photothermal therapy, in which Pluronic-PLL acts as the reductant for the formation of AuNPs without the need for an additional reducing agent. Methods: The deposition of AuNPs on the surface of Pluronic-PLL micelles and the thermal response of the system were followed via ultraviolet-visible spectroscopy and dynamic light scattering. Calcein-AM and MTT assays were used to study the cell viability of MDA-MB-231 cells treated with PTX-loaded Pluronic-PLL@Au NPs, and we then irradiated the cells with NIR light. Results: An obvious temperature response was observed for the Pluronic-PLL@Au NPs. Blood compatibility and in vitro cytotoxicity assays confirmed that the Pluronic-PLL@Au NPs have excellent biocompatibility. Compared to Taxol, the PTX-loaded Pluronic-PLL@Au NPs exhibited higher cytotoxicity in MDA-MB-231 cells. All of these results and confocal laser scanning microscopy analysis results suggest that Pluronic-PLL@Au NPs greatly enhance the cellular uptake efficiency of the drug. Conclusion: As confirmed by in vitro and in vivo studies, the combination of chemotherapy and photothermal therapy can cause more damage than chemo- or photothermal therapy did alone, demonstrating the synergistic effect of chemo-photothermal treatment. Thus, the as-prepared Pluronic-PLL@Au NPs are promising for chemo-photothermal therapy.
研究领域:
纳米技术
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药物递送
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癌症治疗
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材料科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2017
·Vol. 22
·期 11
·1814
·
IF 2.861
Wenrui Wang,Lingyu Zhang,Tiantian Chen,Wen Guo,Xunxia Bao,Dandan Wang,Baihui Ren,Haifeng Wang,Yu Li,Yueyue Wang,Sulian Chen,Baoding Tang,Qingling Yang,Changjie Chen
In this study, resveratrol-loaded solid lipid nanoparticles (Res-SLNs) were successfully designed to treat MDA-MB-231 cells. The Res-SLNs were prepared using emulsification and low-temperature solidification method. The Res-SLNs were spherical, with small size, negative charge, and narrow size distribution. Compared with free resveratrol, the Res-SLNs displayed a superior ability in inhibiting the proliferation of MDA-MB-231 cells. In addition, Res-SLNs exhibited much stronger inhibitory effects on the invasion and migration of MDA-MB-231 cells. Western blot analysis revealed that Res-SLNs could promote the ratio of Bax/Bcl-2 but decreased the expression of cyclinD1 and c-Myc. These results indicate that the Res-SLN may have great potential for breast cancer treatment.Keywords:solid lipid nanoparticle;resveratrol;breast cancer;cyclinD1
研究领域:
药理学
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肿瘤学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2018
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IF 3.095
Shiwei Niu,David H. Bremner,Junzi Wu,Jianrong Wu,Haijun Wang,Heyu Li,Qianqian Qian,Hong Zheng,Limin Zhu
Nanoparticles and macromolecular carriers have been widely used to increase the efficacy of chemotherapeutics, largely through passive accumulation provided by their enhanced permeability and retention effect. However, the therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, the design and development of novel l-peptide functionalized dual-responsive nanoparticles (l-CS-g-PNIPAM-PTX) for active targeting and effective treatment of GRP78-overexpressing human breast cancer in vitro and in vivo are reported. l-CS-g-PNIPAM-PTX NPs have a relative high drug loading (13.5%) and excellent encapsulation efficiency (74.3%) and an average diameter of 275 nm. The release of PTX is slow at pH 7.4 and 25 °C but greatly accelerated at pH 5.0 and 37 °C. MTT assays and confocal experiments showed that the l-CS-g-PNIPAM-PTX NPs possessed high targetability and antitumor activity toward GRP78 overexpressing MDA-MB-231 human breast cancer cells. As expected, l-CS-g-PNIPAM-PTX NPs could effectively treat mice bearing MDA-MB-231 human breast tumor xenografts with little side effects, resulting in complete inhibition of tumor growth and a high survival rate over an experimental period of 60 days. These results indicate that l-peptide-functionalized acid – and thermally activated – PTX prodrug NPs have a great potential for targeted chemotherapy in breast cancer.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Innovative Optical Health Sciences
·
2022
·
IF 2.396
Xiao Ma,Honglian Xiong,Jinhao Guo,Zhiming Liu,Yaru Han,Mingdi Liu,Yanxian Guo,Mingyi Wang,Huiqing Zhong,Zhouyi Guo
Because the breast cancer is an important factor that threatens women’s lives and health, early diagnosis is helpful for disease screening and a good prognosis. Exosomes are nanovesicles, secreted from cells and other body fluids, which can reflect the genetic and phenotypic status of parental cells. Compared with other methods for early diagnosis of cancer (such as circulating tumor cells (CTCs) and circulating tumor DNA), exosomes have a richer number and stronger biological stability, and have great potential in early diagnosis. Thus, it has been proposed as promising biomarkers for diagnosis of early-stage cancer. However, distinguishing different exosomes remain is a major biomedical challenge. In this paper, we used predictive Convolutional Neural model to detect and analyze exosomes of normal and cancer cells with surface-enhanced Raman scattering (SERS). As a result, it can be seen from the SERS spectra that the exosomes of MCF-7, MDA-MB-231 and MCF-10A cells have similar peaks (939, 1145 and 1380 cm − 1 ). Based on this dataset, the predictive model can achieve 95% accuracy. Compared with principal component analysis (PCA), the trained CNN can classify exosomes from different breast cancer cells with a superior performance. The results indicate that using the sensitivity of Raman detection and exosomes stable presence in the incubation period of cancer cells, SERS detection combined with CNN screening may be used for the early diagnosis of breast cancer in the future.
研究领域:
癌症生物学
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生物医学工程
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医学诊断
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机器学习
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产品关联
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2026
·
IF 4.9
Lan Jiang,Lina Zou,Jing Dong,Ping Zhang,Jian Zhou,Hao Jiang,Huanyu Ren,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Feiyan Yang,Lang Cheng,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Non-coding RNAs are critical tumor biomarkers, rendering innovations in their detection technologies highly significant. To overcome the limitations of conventional single-signal electrochemical biosensors (susceptibility to interference, insufficient stability), this study developed a novel ratiometric electrochemical DNA biosensor with dual-signal amplification. Its core novelty is the rational integration of two synergistic nanomaterials: Ferrocene-functionalized MgAl-layered double hydroxide (Fc@MgAl-LDH, as stable immobilization matrix and internal reference signal source) and AuNPs@Fc@MgAl-LDH coupled with MB-cDNA@AuNPs, which construct a cascaded signal amplification pathway. The sensing mechanism involves two-stage amplification: Primary by high-loading-capacity AuNPs@Fc@MgAl-LDH, secondary by MB-cDNA@AuNPs hybridizing with electrode-immobilized microRNA-21 (miRNA-21). The ratiometric signal ( I MB / I Fc ) enables built-in correction, boosting detection accuracy and reproducibility. After systematic optimization, the sensor has a wide linear range (1.0 fM–0.1 nM) and an ultra-low LOD of 0.757 fM, eliminating RNA extraction/enzymatic amplification to simplify operation. Serum validation shows high accuracy (95.3%–101.4% recovery), indicating strong clinical diagnostic application potential.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Songlin Cai,Ciyuan Huang,Caiyun Wang,Libin Zhang,Kai Huang,Haili Dong,Houjin Luo,Kai Chen,Shangfei Yao,Hongxiang Zhu,Ke Sun,Gang Li,Tao Yang,Bo Tang,Bingsuo Zou,Tao Liu
At the present stage, the light sources used in the photocatalytic degradation of organic pollutants are generally UV and simulated sunlight, which invariably increase the extra energy loss. Therefore, it is important to find an effective strategy for the rapid removal of high concentrations of organic pollutants in a cleaner and low-light environment with lower energy consumption. Here we prepare three biochar-based organic photocatalytic materials PM6:TPIC/BC, PM6:TPIC-2Cl/BC, PM6:TPIC-4Cl/BC by impregnation method. Among the three photocatalysts, PM6:TPIC-4Cl/BC exhibits the highest apparent rate constant (0.105 min −1 ), which is 3 times and 1.5 times higher than PM6:TPIC/BC (0.034 min −1 ) and PM6:TPIC-2Cl/BC (0.071 min −1 ) respectively. Due to a wider range of light absorption, the best material is able to completely degrade high concentrations of MB in 3 min. Moreover, photoluminescence (PL), time-resolved photoluminescence (TRPL), electrochemical impedance (EIS), and photocurrent (PCR) measurements confirm its exceptional photogenerated electron-hole separation. Free radical trapping identifies h + , ·OH, and ·O 2 − as key reaction species. DFT analysis reveal atoms with higher Fukui index ( f 0 ) as prime free radical targets. LC-MS detects intermediates, suggesting possible degradation pathways. The reduced toxicity of the degradation products after photocatalysis indicates an environmentally friendly approach. This work demonstrates the valuable of PM6:TPIC-4Cl/BC organic photocatalysts for treating organic wastewater.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Qiqi Wu,Wentao Zhang,Chen Qu,Fei Yuan,Yi Huang,Xianfei Chen,Ruoxi Zhang
The stainless steel wire mesh supported Co 2+ -doped ZnO with lauric acid modification (LA/Co 2+ -ZnO@SSM) was prepared through hydrothermal method for dual-functional oil/water separation performance and high photocatalytic activity. By changing the immersing time of LA, the surface polarity of the LA/Co 2+ -ZnO@SSM composite membrane could be efficiently adjusted from hydrophilic to hydrophobic. The superhydrophobicity of LA/Co 2+ -ZnO@SSM was achieved after LA immersing 120 min, and the WAC value reached as 156.6 ± 3.0°. In different oil/water mixture systems, the LA/Co 2+ -ZnO@SSM composite membrane exhibited excellent oil/water separation ability and separation throughput, and the separation efficiency still remained above 90 % after 10-cycle separation. Meanwhile, the LA/Co 2+ -ZnO@SSM composite also maintained an excellent photocatalytic activity, and the photocatalytic efficiency for methylene blue degradation reached 90 %. Even in the solution of complex organics and oil, the photocatalytic degradation efficiency was still above 75 %. In addition, LA/Co 2+ -ZnO@SSM composite membrane also showed a low adhesion, good flexibility and anti-pressure ability. All results indicated that the dual-functional LA/Co 2+ -ZnO@SSM composite membrane with visible light-driven photocatalysis and oil/water separation performance could be used for practical complex wastewater as a novel purification strategy.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 3
·1461-1471
·
IF 3.361
Wenzhao Han,Fanwei Meng,Hao Gan,Feng Guo,Junfeng Ke,Liping Wang
The treatment of breast cancer mainly relies on chemotherapy drugs, which present significant side effects. The most typical example is the cardiotoxicity and bone marrow suppression associated with doxorubicin (DOX). Therefore, this drug is not the first choice in clinical treatment. We designed ATN-FFPFF-ATN, a new targeted antitumor drug carrier, polymerized from phenylalanine dipeptide (FF), ATN-161 peptide, and Pluronic® F-127. The peptide and Pluronic® F-127 are linked with acetal and are, therefore, acid-sensitive. As cancer can reduce pH through complex mechanisms and subsequently maintain acid ambience, our vehicle can smartly unravel at a peculiar position, through which the drug can specifically accumulate inside the tumor. ATN-161 is a protein ligand of integrin α5β1, which is highly expressed on the surface of some breast cancer cells. This targeting peptide sequence can play a role in the selective delivery of DOX to tumor cells. The DOX-carrying vector was able to significantly inhibit cell proliferation and promote cell apoptosis in MDA-MB-231 cells. Based on these results, ATN-FFPFF-ATN with pH response is a promising vehicle for DOX delivery.
研究领域:
药学
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癌症研究
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 6
·2150-2157
·
IF 4.019
Feiyue Liu,Hui Dong,Yang Tian
As a reactive oxygen species (ROS), peroxynitrite (ONOO−) generated by nitric oxide (NO) and superoxide anion (O2˙−) plays important roles in physiological and pathological processes in the brain. However, the lack of reliable and durable analytical methods in vivo is still a bottleneck to understanding the signal pathway of ONOO− in the brain. In this work, a ratiometric electrochemical biosensor was developed for real-time monitoring and accurate quantification of ONOO− in the rat brain followed by cerebral ischemia. Firstly, a novel organic molecule, 4-(S-(6-mercaptohexyl)benzothioate-6-yl)-7-(diethylamino)-2-(4-(piperazinyl diferroformamide-1-yl)phenyl)chromenylium (HEMF), with a specific recognition group toward ONOO− and ferrocene as an electroactive group, was designed and synthesized for determination of ONOO− with high selectivity. The oxidation peak of ferrocene decreased with increasing concentration of ONOO−, with a rapid response within 15 s, because the pyrylium group in HEMF molecule specifically reacted with ONOO−, resulting in the loss of the ferrocene group from the HEMF molecule through a ring-opening reaction. Meanwhile, 5′-MB-GGCGCGATTTT-SH-3′ (SH-DNA-MB) was optimized as an inner reference molecule, enabling accurate quantification of ONOO− while avoiding environmental effects. The oxidation peak current ratio between ferrocene and MB demonstrated good linearity with concentrations of ONOO− from 20.0 nM to 2.0 μM. The achieved detection limit was as low as 12.1 ± 0.8 nM. The developed biosensor showed remarkable selectivity against potential interferences in the brain, such as other ROS, metal ions, amino acids and bioactive species, due to the specific reaction between the pyrylium group and ONOO−. As a result, the present ratiometric electrochemical biosensor with significant analytical performance, including high temporal resolution, high selectivity and accuracy, combined with the unique characteristics of carbon fiber microelectrode (CFME), such as high spatial resolution and good biocompatibility, was successfully applied in real-time determination of ONOO− in rat brains followed by global cerebral ischemia.
研究领域:
分析化学
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神经科学
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电化学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Qi Qin,Bingwei Yang,Zixuan Liu,Lei Xu,Erqun Song,Yang Song
Polychlorinated biphenyls (PCBs) are persistent industrial pollutants that have been linked to breast cancer progression. However, their molecular mechanism(s) are currently unclear. Our previous assessment suggested that the highly reactive PCB metabolite 2,3,5-trichloro-6-phenyl-[1,4]-benzoquinone (PCB29-pQ) induces the metastasis of breast cancer. Here, our data illustrate that PCB29-pQ increases cancer stem cell (CSC) marker expression, resulting in an increase in the epithelial-mesenchymal transition (EMT) in MDA-MB-231 breast cancer cells; further, the Wnt/β-catenin pathway also becomes activated by PCB29-pQ. When the Wnt/β-catenin pathway is inhibited, the promotion of CSC properties and EMT by PCB29-pQ were accordingly reversed. In addition, the overproduction of reactive oxygen species (ROS) mediated by PCB29-pQ plays a key role in Wnt/β-catenin activation. Collectively, our current data designated the regulatory role of Wnt/β-catenin in PCB29-pQ-triggered acquisition of CSC properties and EMT.
研究领域:
毒理学
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肿瘤学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）