JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
HuaYang Si,Yimeng Chen,Jie Yang,Xiaodong Wen
Although the roots of Achyranthes bidentata (AB), Cyathula officinalis (CO) and Achyranthes aspera (AA) are different drugs, they are always confused in clinical practice due to their similar chemical components and functions. As polysaccharides are abundant in these drugs, a systematic comparison of polysaccharides from AB, CO and AA is not only necessary to understand their similar but not identical functions, but also helpful for the quality control of them. In this study, polysaccharides from 22 batches of AB, CO and AA were compared with monosaccharide composition , molecular weight distribution and saccharide mapping. Polysaccharides of AB, CO and AA had similar monosaccharide compositions but their relative contents of fructose, glucuronic acid , galacturonic acid and glucose were significant different, and could be used as key markers to distinguish them. Results from molecular weight distribution and saccharide mapping showed polysaccharides from AB, CO and AA were mainly composed of fructans with β-2,1 and β-2, 6- D -fructosidic linkages, but their degree of polymerization were different. Meanwhile, pectins were also contained in these three drugs. AB is partial to immunomodulation while CO is partial to removing blood stasis . Fructans and pectins are the similar bioactive substance basis of AB, CO and AA whereas their structural difference might be contributed to the efficacy differentia of these three drugs. This study provides a better understanding on the profiles of polysaccharides from AB, CO and AA, further guiding their clinical usage and facilitating their quality control.
研究领域:
生药学
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药物化学
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中医药学
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产品关联
DOI
PubMed
高影响因子
ANNALS OF BIOMEDICAL ENGINEERING
·
2026
·
IF 5.2
Gao Zanhua,Lin Haoming,Yu Zhifan,Xu Huiqin,Chen Xin,Zhang Xinyu,Shen Yuanyuan
Purpose The development and progression of gliomas are intimately linked to abnormal tumor angiogenesis. Different grades of gliomas exhibit distinct vascular distribution and structural characteristics. Therefore, analyzing vascular architecture and hemodynamics can provide crucial insights into tumor growth and invasion. Methods Ultrasound localization microscopy (ULM) was applied to image the cerebrovascular changes in an orthotopic glioma mouse model at two different stages of tumor progression (14- and 21-days post-implantation). A total of twenty mice with intracranial glioma were imaged on the 14th day (n = 9) and on the 21st day (n = 11) after implantation. Pathological staining, vascular endothelial immunofluorescence examinations, and fluorescence dye perfusion were conducted to compare the ULM imaging results of the microvascular network morphology. Results By separating slow and fast blood flow signals, the ULM images revealed distinct vascular changes that visually correlated with H&E-derived pathological features. The vascular densities in glioma regions showed a significant increase compared to the contralateral side: 30.4% on day 14 (39.91% ± 4.09% vs. 30.60% ± 3.17%, p < 0.001) and 56.5% on day 21 (42.54% ± 3.78% vs. 27.17% ± 4.56%, p < 0.001) post-glioma implantation. The vascular densities obtained from ULM images correlated strongly and significantly with pathological and vascular perfusion results (CD31 and DiI perfusion). ULM-derived multiparametric analysis further revealed significantly reduced average vessel length in glioma regions at both Day 14 (123.72 ± 28.06 μm vs. 192.90 ± 27.75 μm, p < 0.001) and Day 21 (87.07 ± 22.92 μm vs. 233.79 ± 38.94 μm, p < 0.001), decreased blood flow velocity at Day 21 (3.70 ± 0.66 mm/s vs. 4.64 ± 0.82 mm/s, p < 0.001), and increased flow-orientation heterogeneity at both Day 14 (0.838 ± 0.082 vs. 0.595 ± 0.174, p < 0.001) and Day 21 (0.812 ± 0.086 vs. 0.625 ± 0.083, p < 0.001). Conclusion ULM imaging modality enables in vivo simultaneous structural-functional imaging of glioma microvasculature at micron-level resolution, capturing microcirculatory alterations across glioma regions. Its feasibility and reliability in visualizing vascular abnormalities were validated against ex vivo pathological and vascular perfusion, providing preclinical evidence for research and clinical applications in glioma therapy assessment and aggressiveness monitoring.
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产品关联
DOI
高影响因子
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
·
2025
·
IF 0.9
Jiaojiao Zuo,Jing Kang,Jingjing Hong,Jingwen Li,Yi Fang,Chunyi Liu,Minhao Xie,Zhengping Chen
Dopamine transporter (DAT) is closely associated with neurodegenerative diseases such as Parkinson's disease (PD). To develop an in vivo stable radioligand targeting DAT, we synthesized a novel iodine-131-labeled tropane analog [ 131 I] 1 with deuteration on both the N -fluoropropyl and 2 β -carbomethoxy positions of the tropane scaffold and then biologically compared with the previously reported analog [ 131 I]FP-CIT-d 6 with deuteration only on the N -fluoropropyl group. The radioligand [ 131 I] 1 was obtained via a radioiodine-destannylation reaction with a radiochemical yield of ~95%, a radiochemical purity of > 99% and a molar activity of 12.72 GBq/μmol. [ 131 I] 1 exhibited a high in vitro binding affinity for DAT with an IC 50 value of 2.2 nM. Metabolic stability studies demonstrated that [ 131 I] 1 displayed superior in vivo stability compared with [ 131 I]FP-CIT-d 6 . Biodistribution studies revealed that [ 131 I] 1 had better DAT affinity, specificity, and a higher target-to-background ratio than [ 131 I]FP-CIT-d 6 . Ex vivo autoradiography and blocking experiments validated the selective and reversible binding of [ 131 I] 1 to DAT and superiority to [ 131 I]FP-CIT-d 6 . These preliminary results suggest that deuterated radioligand [ 131 I] 1 , with enhanced in vivo stability and higher target-to-background ratio, is a promising DAT probe, warranting the development and application of 123 I-labeled counterpart ([ 123 I] 1 ) for SPECT imaging in DAT-related disorders.
研究领域:
医学影像学
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神经生物学
·
放射化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Cuiying Lin,Yang Liu,Weiqi Wan,Junbo Gao,Lianli Sun
As the number of tuberculosis cases worldwide has increased, the enzyme hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis ( Mt HGPRT) has emerged as a promising target for drug development because of its role in purine salvage and nucleotide homeostasis. However, the bidirectional catalytic mechanism remains poorly understood, posing challenges for rational drug design. Here, residue-specific functional mapping via systematic mutagenesis coupled with molecular dynamics reveals key residues governing the forward and reverse catalysis. D123 stabilizes α-D-5-phosphoribosyl-1-pyrophosphate binding in the forward reaction, whereas V124 and K154 enhance purine ring stability in the reverse reaction. In addition, D126, V176, L181, and D182 regulate substrate coordination and active site conformation. Cross-species validation confirmed the conserved roles of D123 and V124. These findings provide a structural blueprint for enhancing our understanding of the catalytic mechanism of Mt HGPRT and offer insights into drug design targeting HGPRT-related diseases and anti-tuberculosis therapies.
研究领域:
结构生物学
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生物化学
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分子生物学
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药物研发
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微生物学
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计算生物学
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产品关联
DOI
PubMed
高影响因子
Microsystems & Nanoengineering
·
2023
·Vol. 9
·期 1
·1-11
·
IF 7.9
Yang Zekun,Duan Qikai,Zang Junbin,Zhao Yunlong,Zheng Weihao,Xiao Ran,Zhang Zhidong,Hu Liangwei,Wu Guirong,Nan Xueli,Zhang Zengxing,Xue Chenyang,Gao Libo
Recently, flexible iontronic pressure sensors (FIPSs) with higher sensitivities and wider sensing ranges than conventional capacitive sensors have been widely investigated. Due to the difficulty of fabricating the nanostructures that are commonly used on electrodes and ionic layers by screen printing techniques, strategies for fabricating such devices using these techniques to drive their mass production have rarely been reported. Herein, for the first time, we employed a 2-dimensional (2D) hexagonal boron nitride ( h -BN) as both an additive and an ionic liquid reservoir in an ionic film, making the sensor printable and significantly improving its sensitivity and sensing range through screen printing. The engineered sensor exhibited high sensitivity (S min > 261.4 kPa −1 ) and a broad sensing range (0.05–450 kPa), and it was capable of stable operation at a high pressure (400 kPa) for more than 5000 cycles. In addition, the integrated sensor array system allowed accurate monitoring of wrist pressure and showed great potential for health care systems. We believe that using h -BN as an additive in an ionic material for screen-printed FIPS could greatly inspire research on 2D materials for similar systems and other types of sensors. Hexagonal boron nitride ( h -BN) was employed for the first time to make iontronic pressure sensor arrays with high sensitivity and a broad sensing range by screen printing.
研究领域:
材料科学
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电子工程
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生物医学工程
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DOI
高影响因子
EUROPEAN RADIOLOGY
·
2023
·Vol. 34
·期 1
·226-235
·
IF 5.9
Feng Xiao-Lan,Qi Wan-Yin,Xiao Zheng-Yuan,Zheng Xue,Zhang Xiao-Yong,Liu Tao,Kou Xing-Yuan,Chen Jing
Objectives To evaluate the early prevalence of anthracycline-induced cardiotoxicity (AIC) and anthracycline-induced liver injury (AILI) using T2 and T2* mapping and to explore their correlations. Materials and Methods The study included 17 cardiotoxic rabbits that received weekly injections of doxorubicin and magnetic resonance imaging (MRI) every 2 weeks for 10 weeks. Cardiac function and T2 and T2* values were measured on each period. Histopathological examinations for two to five rabbits were performed after each MRI scan. The earliest sensitive time and the threshold of MRI parameters for detecting AIC and AILI based on these MRI parameters were obtained. Moreover, the relationship between myocardial and liver damage was assessed. Results Early AIC could be detected by T2 mapping as early as the second week and focused on the 7th, 11th, and 12th segments of left ventricle. The cutoff value of 46.64 for the 7th segment had the best diagnostic value, with an area under the curve (of 0.767, sensitivity of 100%, and specificity of 52%. T2* mapping could detect the change in iron content for early AIC at the middle interventricular septum and AILI as early as the sixth week ( p = 0.014, p = 0.027). The T2* values of the middle interventricular septum showed a significant positive association with the T2* values of the liver ( r = 0.39, p = 0.002). Conclusion T2 and T2* mapping showed value one-stop assessment of AIC and AILI and could obtain the earliest MRI diagnosis point and optimal parameter thresholds for these conditions. Clinical relevance statement Anthracycline-induced cardiotoxicity could be detected by T2 mapping as earlier as the second week, mainly focusing on the 7th, 11th, and 12th segments of left ventricle. Combined with T2* mapping, hepatoxicity and supplementary cardiotoxicity were assessed by one-stop scan. Key Points • MRI screening time of cardiotoxicity was as early as the second week with focusing on T2 values of the 7th, 11th, and 12th segments of left ventricle. • T2* mapping could be used as a complement to T2 mapping to evaluate cardiotoxicity and as an effective index to detect iron change in the early stages of chemotherapy. • The T2* values of the middle interventricular septum showed a significant positive association with the T2* values of the liver, indicating that iron content in the liver and heart increased with an increase in the chemotherapeutic drugs.
研究领域:
肿瘤心脏病学
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放射学
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分子影像学
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毒理学
·
临床前研究
✓ 已验证
DOI
高影响因子
ACS Sensors
·
2026
·
IF 10.9
Xu Wen-qing,Di Xin-peng,Yuan Wen-qiang,Zhang Zi-ang,Zhou Quan,Lv Pei-yu,Li Xiang-yu,Yang Li-mei,Pan Ge-bo
Flexible capacitive tactile sensors for human biophysical signal monitoring require the simultaneous realization of high sensitivity and a wide detection range, which remains challenging due to the intrinsic structural characteristics of the dielectric layer. Addressing this challenge requires new structural strategies that integrate compliant deformation with mechanical robustness. Here, we present a bioinspired flexible tactile sensor featuring a hierarchical concave microstructure derived from octopus suckers, fabricated via a facile and scalable template-transfer approach that enables precise structural replication and tunable sensing characteristics. Finite element simulations and mechanical characterization were employed to elucidate the pressure-dependent deformation behavior. The results reveal a graded deformation mechanism, where the peripheral rim and central protrusion engage sequentially to delay structural saturation and redistribute stress. As a result, the sensor exhibits segmental sensitivity and high linearity across a wide range of pressures, achieving 8.78 kPa –1 (0–5 kPa), 1.84 kPa –1 (5–230 kPa), and 0.91 kPa –1 (230–500 kPa), with corresponding linearities of 99.34, 97.83, and 98.98%, respectively. Furthermore, the device enables multimodal detection of biophysical signals and spatiotemporal reconstruction of pulse waves, advancing their application in noninvasive medical systems.
✓ 已验证
DOI
PubMed
高影响因子
Small Methods
·
2023
·Vol. 7
·期 10
·2300394
·
IF 12.4
Yuhao Geng,Haoyang Hu,Yongqi Jia,Xintong Huang,Tian Yang,Runzhe Liang,Zhuo Chen,Zhihong Yuan,Jianhong Xu
Lead halide perovskite nanocrystals (LHP NCs) have the characteristics of fast reaction kinetics and crystal instability due to the intrinsically highly ionic bonding between the respective ions, which bring challenges for revealing the growth kinetics and practical applications. Compared with conventional batch synthesis methods, the single-function microreactor can achieve precise and stable control of the NCs synthesis process, but it still has the shortcoming of not being able to obtain information about the growth process. In this study, a micro Total Reaction System (μTRS) with remote control, online detection, and rapid data analysis functions is designed. μTRS can sample the photoluminescence information of CsPbBr 3 NCs growth in ligand-assisted reprecipitation method. CsPbBr 3 NCs with an emission range of 435–492 nm are successfully detected, which breaks the record of the smallest size of CsPbBr 3 NCs synthesized directly from precursors. The real-time feature of μTRS enables the construction of an automated close-loop synthesis system. Besides, the rapid acquisition and timely processing of product information enable the rapid mapping of the operation space for CsPbBr 3 NCs preparation, which provides a reliable and learnable data set for designing a fully autonomous microreaction system capable of synthesizing NCs.
研究领域:
材料科学
·
化学工程
·
纳米技术
·
光电子学
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DOI
高影响因子
NANOTECHNOLOGY
·
2013
·Vol. 24
·期 36
·365101
·
IF 3.842
Fangkai Du,Yunhao Ming,Fang Zeng,Changmin Yu,Shuizhu Wu
Intracellular pH plays a critical role in the function of cells, and its regulation is essential for most cellular processes. In this study, we demonstrate a fluorescence resonance energy transfer (FRET)-based ratiometric pH nanosensor with carbon-dot (CD) as the carrier. The sensor was prepared by covalently linking a pH-sensitive fluorescent dye (fluorescein isothiocyanate, FITC) onto carbon-dot. As the FRET donor, the carbon-dot exhibits bright fluorescence emission as well as λex-dependent photoluminescence emission, and a suitable excitation wavelength for the donor (CD) can be chosen to match the energy acceptor (fluorescein moiety). The fluorescein moieties on a CD undergo structural and spectral conversion as the pH changes, affording the nanoplatform a FRET-based pH sensor. The CD-based system exhibits a significant change in fluorescence intensity ratio between pH 4 and 8 with a pKa value of 5.69. It also displays excellent water dispersibility, good spectral reversibility, satisfactory cell permeability and low cytotoxicity. Following the living cell uptake, this nanoplatform with dual-chromatic emissions can facilitate real-time visualization of the pH evolution involved in the endocytic pathway of the nanosensor. This reversible and low cytotoxic fluorescent nanoplatform may be highly valuable in a variety of biological studies, such as endocytic trafficking, endosome/lysosome maturation, and pH regulation in subcellular organelles.
研究领域:
生物化学
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细胞生物学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2022
·
IF 6.814
Shu Zhang,Yuewu Li,Rui Dong,Weitao Li,Zhiyu Qian,Yamin Yang
Photodynamic therapy (PDT) elicits cell death , vascular damage , or/and anti-tumor host immune response upon activating the administered photosensitive drug by an appropriate light source. Because PDT is heavily dependent on tissue oxygen (O 2 ) in essence, the concentration-dependent impact of O 2 on tailoring cellular response to PDT remains an in-depth investigation. As a multifaceted modality, optimal combinations of photosensitizer (PS) concentration, light dose, and O 2 delivery are critical to achieve ideal therapeutic outcomes. We herein present a fully integrated all-in-one device for the in vitro assessment of PDT efficacy synchronizing the quantitative control of three PDT disciplines simultaneously, aiming at 1) identifying the influence of varying gaseous microenvironments on PDT; and 2) determining the contribution of each PDT factor and estimating the strength of their synergic effect. The gas-gradient-generating unit for contactless headspace O 2 delivery and spatial light control filtering layer in our device could either work as a stand-alone module or combine to screen a range of experimental PDT parameters. By sweeping a total of 128 conditions over four 5-aminolevulinic acid (5-ALA) concentrations, four light dosages, and eight O 2 levels in one single experiment, we determined the main effects of the three key PDT agents and highlighted the interactive effect between 5-ALA and light after full-factorial statistical analysis. Our device is not only a versatile tool for predicting PDT efficacy during the translational study but also provides valuable multidimensional information for the interrelation between key PDT factors, which may expedite clinical PDT dosimetry and furnish new insights for the fundamental understanding of photobiological processes.
研究领域:
生物医学工程
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癌症研究
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治疗模式
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生物物理学
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Yiwei Fang,Zhiliang Chen,Jinyu Chen,Minqi Zhou,Yuanyao Chen,Rong Cao,Chunyan Liu,Kai Zhao,Min Wang,Huiping Zhang
Mono(2-ethylhexyl) phthalate (MEHP) is a metabolite of DEHP which is one of phthalic acid esters (PAEs) widely used in daily necessities. Moreover, MEHP has been proven to have stronger biological toxicity comparing to DEHP. In particular, several recent population-based studies have reported that intrauterine exposure to MEHP results in adverse pregnancy outcomes. To explore the mechanisms and metabolic biomarkers of MEHP exposure, we examined the metabolic status of HTR-8/Svneo cell lines exposed to different doses of MEHP (0, 1.25, 5.0, 20 μM). Global and dose–response metabolomics tools were used to identify metabolic perturbations and sensitive markers associated with MEHP. Only 22 metabolic features (accounted for <1 %) were significantly changed when exposed to 1.25 μM. However, when the exposure dose was increased to 5 or 20 μM, the number of significantly changed metabolic features exceeded 300 (approximately 10 %). In particular, amino acid metabolism, pyrimidine metabolism and glutathione metabolism were widely affected according to the enrich analysis of those significant altered metabolites, which has and have previously been reported to be closely related to fetal development. Moreover, 5′-UMP and N -acetylputrescine with the lowest effective concentrations (EC −10 = 0.1 μM and EC +10 = 0.11 μM, respectively) were identified as sensitive endogenous biomarkers of MEHP exposure.
研究领域:
毒理学
·
生殖生物学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
Chinese Medicine
·
2022
·Vol. 17
·期 1
·1-13
·
IF 4.546
Chen Zherui,Zhu Baojie,Chen Zhixin,Cao Wen,Wang Junqiao,Li Shaoping,Zhao Jing
Background Polygonatum cyrtonema, one of origins of Polygonata Rhizoma ( HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China. However, there has no standard for steaming of HuangJing . Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema . Methods To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0–5 times, respectively. Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively. In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared. Results Results suggested that molecular weights could be changed during steam, which increased by first steaming and then decreased with further steaming though all polysaccharides’ molecular weights were 10 5 -10 7 Da. They all showed irregularly spherical conformation in aqueous solution based on AFM imaging. Their monosaccharide composition and PACE fingerprints were significantly different after steaming, i.e., galactose increased while glucose and mannose decreased, and β-1,4-Gal p appeared while β-1,4-Man p increased, after steaming. Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis. Conclusions In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema . Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
研究领域:
中药学
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生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）