FOOD CHEMISTRY
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2023
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IF 8.8
Yan Wu,Yue Zhou,Hongchen Long,Xiangyu Chen,Yuanyuan Jiang,Lei Zhang,Tao Le
The study investigates a bimetallic organic framework (Zn/Eu-MOF) based fluorescent probe for visual detection of ofloxacin (OFL) in pork, beef and fish. The developed sensing probe recognizes OFL through internal filtration and cation-π interaction between OFL and Zn/Eu-MOF, resulting in a distinct color change from orange-red to light green. The content of OFL can be determined through RGB analysis by a mobile-phone. The developed sensing probe offers several advantages such as broad linear range (0.1 ∼ 80 μM), rapid response time (30 s), low detection line (0.44 μM). The effectiveness of the sensing probe can last for five rounds with good recovery. Moreover, the application of the sensing probe on pork, beef and fish samples are reliable, with recoveries ranging from 93.4 to 112.1%, and the relative standard deviations (RSD) within 1.17% to 2.06%. These results suggest that the developed sensing probe could have significant potential for practical on-site test in food.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Zhao-Hong Hu,Yan–Fei Wang,Ahmed Mohamed Omer,Xiao–kun Ouyang
A novel adsorbent with high selectivity for fluoroquinolone (FQ) compounds was developed, based on the surface functionalization of magnetic carboxylated cellulose nanocrystals (M-CCNs) with molecularly imprinted polymer (MIP) comprising amine moieties. The imprinting was achieved by a radical polymerization technique, which uses glycidyl methacrylate, tetraethylenepentamine, ofloxacin, ethylene glycol dimethacrylate, and azobisisobutyronitrile as the functional monomer, active groups provider, template molecule, crosslinking agent, and initiator, respectively. The developed material (M-CCNs@MIP) was comprehensively characterized and shown to exhibit high adsorption capacity and selectivity with rapid equilibration time. Moreover, the adsorption isotherms could be well-fitted with the Freundlich model, and the adsorption kinetics followed the pseudo-second-order model. The maximum adsorption capacities for M-CCNs@MIP after 2 and 20 min were 34.09 and 40.65 mg g −1 , respectively, compared to 9.98 and 15.28 mg g −1 observed for the unimprinted polymer (M-CCNs@NIP). By coupling the M-CCNs@MIP adsorbent with high-performance liquid chromatography, an approach was established to enhance the selective adsorption of seven structurally similar FQ compounds in river water samples. The recoveries of the seven FQs ranged from 81.2 to 93.7%, and the limits of detection were between 5.4 and 12.0 ng mL −1 . The M-CCNs@MIP adsorbent also retained good performance after seven consecutive cycles of reuse.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
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IF 9
Haoruo Xu,Chenxi Huang,Weili Yu,Ningjie Fang,Yinghao Chu,Jinhui Li
The combined pollution toxicity of hexavalent chromium heavy metal (Cr(VI)) and antibiotics (such as ofloxacin, OFL) is much higher than that of a single pollutant, posing a serious challenge to the ecological environment. Photocatalytic technology can achieve the removal of both single pollutants and composite pollutants. It is urgent to develop catalysts with high carrier separation efficiency and photocatalytic degradation performance. In this work, we successfully prepared m-PDI/BiOI heterojunction catalysts (mPB-3) with tight contact interfaces using the continuous ion layer adsorption and reaction method, and applied them to the synergistic removal of OFL and Cr(VI). The results showed that mPB-3 exhibited a bidirectional enhancement effect. The degradation rate of OFL reached more than 99.5 % within 40 min, the corresponding reduction efficiency of Cr(Ⅵ) reached 63.7 % at pH = 9. Furthermore, the influence of different environmental factors on the degradation process have also been clarified, indicating that mPB-3 has good stability and adaptability. The system characterization results confirmed that a S-scheme heterojunction has been formed. This can accelerate the rapid migration of electrons from m-PDI to BiOI, achieving efficient charge separation, and ultimately enhancing the photocatalytic activity. Finally, the degradation intermediate products and degradation mechanism were analyzed. This study provides a feasible solution for the design of efficient photocatalytic materials and their application in the combined pollution treatment of OFL and Cr(VI). Environmental implication Cr(VI) and OFL coexisting in water bodies pose significant ecological risks due to their enhanced combined toxicity. Traditional treatment techniques often struggle to effectively address such complex pollution systems. This study demonstrates that the m-PDI/BiOI heterojunction photocatalyst can efficiently synergistically remove both contaminants under environmentally relevant conditions (e.g., pH = 9), providing a practical strategy to mitigate composite pollution challenges. The S-scheme charge separation mechanism offers insights for designing advanced catalysts to tackle emerging multi-pollutant threats in aquatic ecosystems, supporting sustainable water treatment solutions.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
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IF 6.6
Yurou Jiang,Di Lan,Qian Liu,Yong Jiang
NPs and OFL trigger systemic transcriptional suppression in Synechococcus. • Combined exposure drives severe oxidative burst and ultrastructural damage. • Stress shifts cells from “compensatory repair” to total metabolic collapse. • Photosynthetic impairment disrupts carbon–nitrogen metabolic coupling. The co-occurrence of nanoplastics (NPs) and antibiotics in marine environments poses complex threats to primary producers, yet the multi-level mechanisms of their combined toxicity remain poorly understood. In this study, we systematically investigated the physiological, biochemical, and transcriptomic responses of the marine cyanobacterium Synechococcus sp. WH8102 to single and combined exposure of polystyrene NPs and ofloxacin (OFL). Interestingly, Independent Action analysis based on population growth indicated an apparent antagonistic interaction, which may be associated with altered OFL availability in the presence of NPs. In contrast, ultrastructural and biochemical analyses revealed pronounced cellular damage under combined exposure. The adherence of NPs to the cell surface was accompanied by compromised membrane integrity, elevated ROS accumulation, and enhanced cellular impairment. This extreme oxidative stress triggered a systemic collapse of the photosynthetic apparatus, drastically inhibiting photochemical efficiency. Consequently, the resulting ATP and NADPH supply crisis propagated to the core metabolic networks, severely inhibiting key enzymes in both carbon fixation and nitrogen assimilation. Transcriptomic profiling further elucidated a critical regulatory shift: whereas single OFL exposure induced compensatory repair mechanisms—upregulating carbon metabolism and ribosomal genes—combined stress suppressed these defenses. This forced a global transcriptional shutdown of photosynthesis, the TCA cycle, and protein synthesis as a failed “energy conservation” strategy. Ultimately, this study highlights the critical disparity between macroscopic population antagonism and microscopic cellular impairment, providing profound mechanistic insights into the profound ecological risks of combined emerging contaminants. Download: Download high-res image (122KB) Download: Download full-size image
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CHEMICAL PAPERS
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2026
·
IF 2.7
Wu Yanlin,Qin Pixian,Li Yuan,Dong Wenbo,Monfort Olivier,Zeng Yilan
In this study, trace chlorogenic acid (CGA) was introduced as a natural reductant and ligand to facilitate the activation of Fe(III) in a sulfate radical-based advanced oxidation process (SR-AOP), enabling effective Fenton-like degradation of ofloxacin (OFL) under mild conditions. The CGA/Fe(III)/PMS system exhibited excellent performance in the degradation of ofloxacin (OFL) at near-neutral pH. The presence of CGA effectively promoted Fe(III)/Fe(II) cycling via dual redox and chelation effects, thereby enhancing PMS activation. Reactive oxygen species (ROS) analysis suggested that sulfate radicals (SO 4 ·− ) were the dominant contributors to OFL degrdation, while other reactive species played minor roles. The system maintained high activity in the presence of common coexisting anions in water (e.g., Cl − , and SO 4 2− ), whereas HCO 3 − and HPO 4 2− significantly suppressed degradation. These findings demonstrated the promising potential of CGA as an environmentally compatible additive for Fe(III)-based AOPs, providing useful mechanistic insight for mitigating antibiotic contamination in aquatic environments. Graphical abstract
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xuhui Yue,Hongxia Zhang,Jiangxiong Zhu,Shaokang Zhang,Naifeng Xu,Yuanfeng Wang
Ofloxacin (OFL), a third-generation quinolone antibiotic, has been reported to cause adverse multisystem reactions in humans after residual ingestion. The aim of this study was to develop a gold core-platinum shell nanozymes (Au@Pt NPs) via a layer-by-layer seed growth method for the rapid detection of OFL. The synthesized Au@Pt NPs, with a 25 nm gold core and 6 platinum shells, exhibited robust peroxidase-like activity. Kinetic analysis revealed a Michaelis constant (Km) of 0.41 ± 0.04, a catalytic constant (Kcat) of 1.41 × 10 5 , and a catalytic efficiency (Kcat/Km) of 3.40 × 10 5 . An indirect competitive Enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip were developed based on these nanozymes. The ic-ELISA demonstrated a linear range of 0.2–7.35 ng/mL, an IC 50 of 0.96 ng/mL, and a limit of detection (LOD, IC 10 ) of 0.03 ng/mL. The immunochromatographic strip exhibited a detection range of 0.060–4.512 ng/mL, an IC 50 of 0.521 ng/mL, and an LOD of 0.017 ng/mL, with a detection time of 5–7 min. Applied to real samples, the method achieved recoveries of 86.48 %-116.96 % in fish and 73.90 %-123.37 % in chicken, with relative standard deviations (RSDs) below 20 %. Therefore, this Au@Pt NP-based immunoassay demonstrated a rapid, sensitive, and specific method for screening antibiotic residues in food.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Huayi Cai,Jingjing Wang,Zhao Du,Zhiyuan Zhao,Yaxin Gu,Zhonglu Guo,Yang Huang,Chengchun Tang,Guifeng Chen,Yi Fang
Photocatalysis has attracted much attention in the field of environmental remediation. However, the photocatalytic performance largely depends on efficient charge migration and solar light utilization. Herein, the ternary MoSe 2 /ZnO/p-BN (MZB) photocatalyst was successfully designed and synthesized. The degradation rate of the optimal sample 30 wt% MZB for ofloxacin (OFL) under visible light irradiation can reach 99.2%, and the photocatalytic reaction rate is 5.33 times and 2.10 times higher than that of pure ZnO and ZnO/p-BN. In the MoSe 2 /ZnO/p-BN system, p-BN can achieve the enrichment of pollutant to accelerate the catalytic rate, while the hybridization of MoSe 2 expands the spectral response of the catalysts. In addition, the formation of stepped electron transport routes reduces the electron-hole pair recombination rate. The active species quenching experiments reveal that e - , •O 2 − , h + and •OH play a major role in the removal of antibiotic contaminants. The analysis of OFL degradation intermediates reveals the possible degradation pathways and proposes a reasonable photocatalyst mechanism. Therefore, this work provides new ideas for designing high-performance visible light responsive photocatalysts for pollution treatment.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Zhou,Yue Huang,Qian Wang,Yijie Wang
Antibiotic residue in food is still a serious health hazard to human with the various and severe adverse effects, so ultrasensitive detection of antibiotic residue becomes increasingly significant. Herein, a homogenous electrochemical biosensor is established for ultrasensitive detection of antibiotic ofloxacin (OFL) based on AuNPs-enhanced synergistic amplification of dual enzymes. Target OFL is ingeniously designed to initiate the synergistic effect of exonuclease III-catalyzed DNA cycle and terminal deoxynucleotidyl transferase-mediated polymerization, producing a great many polyA@AuNPs which subsequently adsorb onto the surface of gold electrode and finally lead to highly amplified electrochemical signal by binding with numerous electroactive indicator RuHex. Benefiting from the collaboratively multiple signal amplifications enhanced by AuNPs with good electrical conductivity and probe loading capacity, the constructed biosensor exhibits excellent quantitative detection performance for OFL with a linear range of 0.008 to 100 ng mL −1 and a limit of detection as low as 2.73 pg mL −1 . Furthermore, the detection system accomplished in homogeneous solution without complex chemical modification and rebuilding of sensing architectures on the electrode endows the biosensor with the advantages of convenient operation, good reproducibility and stability. Moreover, the biosensor shows satisfactory analytical performance for OFL detection in real food samples, providing a potential approach for practical application.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Can Li,Shuangshuang Cui,Jian Zhang,Yunfeng Zhao,Xiayu Peng,Fengxia Sun
In this paper, Prussian blue nanoparticles (PBNPs) and colloidal gold (AuNPs) were synthesized by solvothermal method and trisodium citrate reduction method respectively, and two lateral flow immunoassay (LFIA) methods for the detection of ofloxacin (OFL) in fish were established. The larger particle size and superior optical properties of PBNPs make the sensitivity of PBNPs-LFIA higher than that of AuNPs-LFIA. In the process of establishing the assay, we found that the amount of antibody required for PBNPs-LFIA is 1/3 of that for AuNPs-LFIA, and the amount of probe is 1/2 of that for AuNPs-LFIA, suggesting that PBNPs-LFIA is able to save the amount of antibody and greatly reduce the cost of the assay.The detection limit of PBNPs-LFIA for OFL was 0.064 ng mL -1 , which was 2.3 times lower than that of AuNPs-LFIA, and the visual detection limit and elimination value of PBNPs-LFIA were also lower than that of AuNPs-LFIA.In addition, the recoveries of two established LFIAs for the determination of OFL in fish were 84.8%-116.3%, and the coefficient of variation was less than 10%. Therefore, PBNPs are a potential LFIA probe marker.
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JOURNAL OF FLUORESCENCE
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2025
·
IF 3.1
Idan Elaf W.,Qasim Bashar,Jeber Jalal N.
This study introduces an innovative fluorescence enhancement strategy utilizing carbon quantum dots (CQDs) for the ultrasensitive and selective detection of Ofloxacin in pharmaceutical formulations. Synthesized hydrothermally from ascorbic acid, a green, non-toxic precursor, the CQDs exhibit a unique "turn-on" response upon interaction with Ofloxacin, contrasting conventional quenching-based mechanisms. The fluorescence enhancement (22.4% quantum yield) arises from Ofloxacin-induced surface passivation of CQDs, which reduces non-radiative decay pathways, as evidenced by lifetime studies and spectroscopic characterization. Optimized synthesis conditions (180 °C, 8 h) yielded monodisperse CQDs (17–30 nm) with tailored optical properties, validated via TEM, FTIR, XPS, and XRD. Under optimal detection parameters (pH 8.0, 40 °C, 800 µL CQDs), the method achieved a wide linear dynamic range of 0.5–120 µM (y = 0.0186x + 1.0023, R 2 = 0.9987), with detection and quantification limits of 75 nM and 248 nM (3σ/S and 10σ/S criteria), surpassing HPLC sensitivity by twofold. The quantum yield increased from 22.4% (pristine CQDs) to 28.9% upon Ofloxacin binding, confirming the turn-on mechanism. Rigorous validation per United States Pharmacopeia guidelines demonstrated high accuracy (mean recovery: 100.5 ± 3.4%; range: 96.2–104.8%) and precision (intra-day RSD < 1.8%, inter-day RSD < 2.5%, n = 6). The eco-friendly protocol eliminates organic solvents, aligning with green chemistry principles, while maintaining stability for four weeks at 4 °C. By pioneering a cost-effective, sustainable "turn-on" fluorescence platform, this work addresses critical challenges in pharmaceutical quality control and extends potential applications to clinical diagnostics and environmental monitoring of antibiotic residues.
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Scientific Reports
·
2021
·Vol. 11
·期 1
·1-12
·
IF 4.38
Yang Chenghu,Miao Shichao,Li Tiejun
The influences of water washing treatment on the properties of Ulva prolifera -derived biochar ( U.P- biochar) and its sorption characteristics of ofloxacin (OFL) were investigated. The results showed that the water washing treatment significantly changed the physiochemical structures of U.P- biochars, and improved the sorption capacity of OFL. The sorption capacity of OFL by U.P -biochar was closely dependent on pyrolysis temperature (200–600 °C) and equilibrium solution pH (3–11). Different sorption mechanisms (e.g. cation exchange, electrostatic attraction, H-bond and cationic–π and π–π interactions) were dominant for specific U.P- biochars under various pH regions (acidic, neutral and alkaline). Moreover, the unwashed and washed U.P- biochars prepared at 200 °C (BC200 and BCW200) showed a higher sorption capacity of OFL at pH = 7. The two-compartment first-order model provided an appropriate description of the sorption kinetics of OFL by BC200 and BCW200 ( R 2 > 0.98), which revealed that the contribution ratios between the fast and slow sorption compartments ( f fast /f slow , 1.55 for BC200 and 1.25 for BCW200) reduced after water washing treatment of U.P -biochar. The values of n for the Freundlich model were less than 1, which demonstrated that the sorption of OFL by BC200 and BCW200 was favourable and nonlinear. Also, the sorption of OFL by BC200 and BCW200 increased with an increase in solution temperature and the sorption process was spontaneous and endothermic. This study provides valuable information for being a primary consideration in the production and application of U.P- biochar.
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FOOD AND AGRICULTURAL IMMUNOLOGY
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2017
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IF 1.392
Xiujin Chen,Zhaozhou Li,Jinying Guo,Daomin Li,Hongli Gao,Yao Wang,Chuanlai Xu
A broad-specificity immunoassay was established to monitor marbofloxacin (MBF) and ofloxacin (OFX) residues in raw milk and porcine muscle samples. The main influential parameters in the assay buffer were investigated to improve the assay performance. The cross-reactivities (CRs) were lower than 0.25%, except for OFX (41.38%). The quantitative working ranges for MBF and OFX based on fortified matrices ranged from 0.11 to 30.85 ng mL−1, with a 50% inhibitory concentration of 0.76±0.12 ng mL−1 for MBF and 2.70±0.28 ng mL−1 for OFX. The detection limits of enzyme-linked immunosorbent assay (ELISA) ranged from 0.053 to 0.358 ng mL−1 in spiked samples. The mean recoveries were in the range of 80.52–95.46%, less than 12.06% of the coefficient of variation. A good correlation between the ELISA results and those from instrument methods demonstrated the practical application of the novel ELISA. Therefore, the immunoassay proposed is feasible for MBF and OFX analysis in animal-derived foods.
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