Biomaterials Science
·
2017
·Vol. 5
·期 11
·2268-2275
·
IF 4.21
Haobo Han,Jiebing Yang,Yudi Wang,Wenqi Chen,Jiawen Chen,Yan Yang,Quanshun Li
The nucleobase analogue 2-amino-6-chloropurine was modified on the surface of polyamidoamine (PAMAM) to construct a derivative AP-PAMAM, and then it was used as a gene carrier for miR-23b delivery to achieve the anti-tumor effects. The carrier AP-PAMAM could condense miR-23b into stable nanoparticles with a particle size of 97.3 nm (N/P ratio of 50), which was favorable for the cellular uptake of nanoparticles. Compared with PAMAM, AP-PAMAM exhibited an obviously enhanced transfection efficiency through the transfection assay of plasmids pEGFP-N3 and pGL-3. Using the human lung adenocarcinoma cell line A549 as a model, AP-PAMAM-mediated miR-23b delivery could achieve a stronger anti-proliferative effect than PAMAM/miR-23b. The inhibition of cell proliferation was elucidated to be associated with the apoptotic induction (apoptotic ratio of 23.2%) and S phase arrest owing to the decreased expression level of cyclin D1. Meanwhile, the AP-PAMAM-mediated miR-23b delivery could suppress the cell migration and invasion of cancer cells through wound healing and Transwell migration assays. In summary, the PAMAM derivative-mediated miR-23b delivery could be a promising strategy for achieving tumor gene therapy.
研究领域:
药学
·
分子生物学
·
癌症研究
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2011
·
IF 5.361
Yarui An,Xiaoli Jiang,Wenji Bi,Hua Chen,Litong Jin,Shengping Zhang,Chuangui Wang,Wen Zhang
A novel electrochemical immunosensor for sensitive detection of α-synuclein (α-SYN), a very important neuronal protein, has been developed based on dual signal amplification strategy. Herein, G4-polyamidoamine dendrimer-encapsulated Au nanoparticles (PAMAM-Au nanocomposites) were covalently bound on the poly- o -aminobenzoic acid (poly- o -ABA), which was initially electropolymerized on the electrode surface to perform abundant carboxyl groups. The formed immunosensor platform, PAMAM-Au, was proved to provide numerous amino groups to allow highly dense immobilization of antigen, and facilitate the improvement of electrochemical responses as well. Subsequently, the enhanced gold nanoparticle labels ({HRP-Ab 2 -GNPs}) were fabricated by immobilizing horseradish peroxidase-secondary antibody (HRP-Ab 2 ) on the surface of gold nanoparticles (GNPs). After an immunoassay process, the {HRP-Ab 2 -GNPs} labels were introduced onto the electrode surface, and produced an electrocatalytic response by reduction of hydrogen peroxide (H 2 O 2 ) in the presence of enzymatically oxidized thionine . On the basis of the dual signal amplification of PAMAM-Au and {HRP-Ab 2 -GNPs} labels, the designed immunosensor displayed an excellent analytical performance with high sensitivity and stability. This developed strategy was successfully proved as a simple, cost-effective method, and could be easily extended to other protein analysis schemes.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Materials Interfaces
·
2017
·Vol. 4
·期 24
·1701166
·
IF 4.279
Qianyu Zhang,Li Wang,Yajun Jiang,Wei Gao,Yinsong Wang,Xiaoying Yang,Xinlin Yang,Zunfeng Liu
The multifunctional nanocomposites with grafting polyamidoamine dendrimers up to third generation (G 3 ) are grown onto the surface of mesoporous silica-coated gold nanorods (AuNRs@SiO 2 ) via a divergent technique. The resultant AuNRs@SiO 2 -G 3 nanocomposites with uniform size and excellent stability not only enable their utilization as targeted contrast agents for photothermal cancer therapy but also serve as scaffolds for the intracellular delivery of anticancer drug and small interfering RNA (siRNA) to enhance the efficiency of cancer therapy. The controlled doxorubicin (DOX) release from AuNRs@SiO 2 -G 3 nanocomposites is significantly improved under lower pH condition and near-infrared laser irradiation. The Bcl-2-targeted siRNA is transfected into tumor cells and induces knockdown of the protein expression, which is confirmed by western blot assays. Furthermore, the complementary effect of chemo- and gene therapy to MCF-7 cells for improved photothermal therapy is demonstrated by MTT assay. The DOX and siRNA coloaded AuNRs@SiO 2 -G 3 nanocomposites show much lower cytotoxicity compared to the DOX, resulting in low toxicity to normal tissues. The multifunctional nanocomposites have potential application as nanoplatforms to integrate the photothermal-chemo-gene tumor therapy with high efficiency.
研究领域:
纳米医学
·
肿瘤学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Dandan Guo,Chaoyan Lou,Nani Wang,Meilan Chen,Peimin Zhang,Shuchao Wu,Yan Zhu
A novel high performance liquid stationary phase was prepared by grafting poly amidoamine (PAMAM) dendrimers to poly (styrene-divinyl benzene-glycidylmethacrylate) matrix in this work. Different generations of PAMAM dendrimers grafted to polymer matrices containing different glycidylmethacrylate amounts by reacting with epoxy groups in glycidylmethacrylate molecules. The synthesized PAMAM dendrimers and stationary phase were characterized by fourier transform infrared spectroscopy, scanning electron microscope, N 2 adsorption/desorption, elemental analysis and thermo gravimetric analyzer. The proposed stationary phase was suitable for rapid separation of phenylene diamine isomers and aminobenzene . The separation mechanism of benzene rings attraction and amino repulsion was verified by retention behaviors of methylamine , phenylamine, phenylene diamine, naphthylamine and toluene. The reliability of the stationary phase was demonstrated by separation and determination of phenylene diamine isomers including m -phenylene diamine, o -phenylene diamine and p -phenylene diamine in hair dye precursors within 10 min. Satisfactory resolution and short analysis time make the columns promising for determination of aniline compounds in various fields.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Xiaoyi Lu,Shangshang Zhang,Weiguang Shi,Dan Cheng,Hong-Wei Li,Shihan Xu,Biao Dong
Carbon dots (CDs) are important zero-dimensional materials with excellent luminescent properties. However, the mechanism of CDs photoluminescence is still an open debate, which significantly hinders the development of CDs with desired optical properties. In this work, bright blue fluorescent carbonized 1.0 G polyamine-amine polymer dots (NCPDs) has been prepared by a simple hydrothermal method, with a quantum yield of 57 %. The dominant luminescence of the NCPDs is attributed to the surface states based on synergistic hybridization between the carbon backbones and connected functional groups at below 120 °C. Photoluminescence transforms from the surface states to the surface - carbon core states, and then to carbon core states from 120° to 150 °C. Meanwhile, the degree of carbonization increases along with the size of NCPDs growing from 1.36 to 3.20 nm, and the NCPDs present excitation-dependent emission properties. Due to the remarkable fluorescence property of NCPDs, Cu 2+ determination has been carried out in the aqueous phase, and the detection limit is 9 nM, which is much lower than the international minimum standard (20 μM). NCPDs present high selectivity and sensitivity toward detecting Cu 2+ , and provide potential applications in detecting metal pollution in the environmental water.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xinran Yang,Zonglin Li,Junhui Du,Xueli Zhang,Haibin Liu,Xuechao Zhang,Lijun Li,Chuanjin Cui,Hongshuo Chen
In this paper, a SERS/electrochemical dual-channel aptamer biosensor was established for ultrasensitive detection of Pb 2+ . Firstly, the capture probe Fe 3 O 4 @Au@PAMAM@Aptamer and the signal probe AuNs@4-MBA@Au@ssDNA were chemically synthesized step by step. Secondly, the carboxyl graphene oxide (GO) was electrodeposited onto the electrode surface to gain the GO/SPCE. Meanwhile, PAMAM@Au nanomaterials were successfully synthesized and thus prepared the Aptamer/PAMAM@Au/GO/SPCE. During the process, SEM, TEM, FTIR and XPS were applied to characterize the synthesized nanomaterials which confirmed the successful synthesis of the nanomaterials. Finaly, the performance of this biosensor was evaluated in a series of Pb 2+ aqueous solutions by surface enhanced Raman (SERS) and differential pulse voltammetry (DPV). The results showed that the lowest limit of detection (LOD) was 3.16 × 10 −5 nM, and the sample recovery was 99.50–103.67 %. Furthermore, the biosensor demonstrated good specificity, repeatability, and stability. Reliable results were also obtained in actual sample testing. Therefore, it can provide a viable method for trace detection of heavy metal ions.
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Guijie Wei,Jianhua Chen,Ziqi Jing,Yanyi Li,Zhihui Li,Wei Zheng,Xiurui Sun,Wenwen Zhao,Zhe Zhang,Xue Wang,Hongcui Han,Chu Li,Yujie Zhang,Pengkai Ma
Mitochondria are appealing targets in cancer therapy for providing a suitable microenvironment and energy supply. Herein, we constructed a glycosylated poly(amido amine)/celastrol (PAMAM/Cel) complex for hypoxia-activated mitochondria-specific drug delivery and chemothermal therapy to inhibit tumor growth and metastasis. The complex was characterized by high photothermal conversion efficiency, hypoxia-sensitive polyethylene glycol (PEG) outer layer detachment, and alkaline-sensitive drug release. The complex showed specific cellular uptake in glucose transporter 1 (GLUT1)-overexpressing tumor cells and mitochondrial accumulation in a hypoxic environment. Combined with near-infrared (NIR) laser irradiation, the complex exhibited higher cytotoxicity, apoptosis induction, and metastasis inhibition rates due to the synergistic chemothermal effect. Similarly, the complex also targeted tumors and accumulated in mitochondria in tumor-bearing nude mice, resulting in superior inhibitory effects on tumor growth and metastasis as well as low systematic toxicity. Further mechanistic studies discovered that the complex impaired the mitochondrial membrane, reduced adenosine triphosphate (ATP) content, and regulated metastasis-related protein expression. Thus, the present study provides a promising nanomedicine for tumor therapy.
研究领域:
药学
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2023
·
IF 2.9
Zhang Wensi,Cui Chuanjin,Hongshuo Chen,Zhang Xuechao,Du Junhui
In this study, a novel electrochemical aptamer sensor for detecting ochratoxin A (OTA) was constructed. First, a gold-copper alloy film was prepared via electrochemical deposition, and copper was selectively dissolved in constant potential mode for obtaining the nano-porous gold modified screen-printed carbon electrodes (NPG/SPCE). Then, 2-mercaptoethylamine was dropped on the NPG/SPCE surface and Au-S covalent bonds were formed for immobilizing the metal. Glutaraldehyde as cross-linking agent was added, which resulted in immobilization and attachment of PAMAM to the 2-mercaptoethylamine through the dehydration condensation reaction. During the preparation process, the nano-porous gold and PAMAM-modified layers were characterized by SEM, XRD, and IR spectroscopy, respectively. The characterization results showed that the nano-porous gold and PAMAM composite films were successfully modified. Finally, the OTA aptamer was cross-linked with PAMAM by glutaraldehyde to complete construction of the Apt/PAMAM/NPG/SPCE sensor. The electrochemical performance of this sensor was tested in ochratoxin A solutions with the DPV method. The results showed that the sensor’s reproducibility, stability, and specificity were good. The spiked recoveries in red wine ranged from 99.65%∼101.6%, with a linear range of 0.5 ng/mL∼20 ng/mL and a minimum detection limit of 0.141 ng/mL. Thus, the novel biosensor may provide a promising tool for the trace detection of OTA.
研究领域:
分析化学
·
生物传感器
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2019
·Vol. 17
·期 1
·1-12
·
IF 5.345
Lai Sisi,Wei Yanyan,Wu Quanwu,Zhou Kang,Liu Tuo,Zhang Yingfeng,Jiang Ning,Xiao Wen,Chen Junjie,Liu Qiuhong,Yu Yang
Background Age-related macular degeneration (AMD) is a leading cause of severe visual deficits and blindness. Meanwhile, there is convincing evidence implicating oxidative stress, inflammation, and neovascularization in the onset and progression of AMD. Several studies have identified berberine hydrochloride and chrysophanol as potential treatments for ocular diseases based on their antioxidative, antiangiogenic, and anti-inflammatory effects. Unfortunately, their poor stability and bioavailability have limited their application. In order to overcome these disadvantages, we prepared a compound liposome system that can entrap these drugs simultaneously using the third polyamidoamine dendrimer (PAMAM G3.0) as a carrier. Results PAMAM G3.0-coated compound liposomes exhibited appreciable cellular permeability in human corneal epithelial cells and enhanced bio-adhesion on rabbit corneal epithelium. Moreover, coated liposomes greatly improved BBH bioavailability. Further, coated liposomes exhibited obviously protective effects in human retinal pigment epithelial cells and rat retinas after photooxidative retinal injury. Finally, administration of P-CBLs showed no sign of side effects on ocular surface structure in rabbits model. Conclusions The PAMAM G3.0-liposome system thus displayed a potential use for treating various ocular diseases.
研究领域:
药学
·
眼科
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2013
·Vol. 5
·期 8
·2801-2808
·
IF 5.231
Yu Zhang,Chunsheng Xiao,Mingqiang Li,Jianxun Ding,Chaoliang He,Xiuli Zhuang,Xuesi Chen
The linear-dendritic prodrug MPEG–b-PAMAM–LA/DOX was synthesized through modifying the linear methoxy poly(ethylene glycol)–block-dendritic poly(amidoamine) (MPEG–b-PAMAM) with lipoic acid (LA) and then conjugating doxorubicin (DOX) by an acid-labile hydrazone bond. The obtained amphiphilic prodrug self-assembled into nanosized spherically shaped micelles in deionized water, as characterized by transmission electron microscopy (TEM) and dynamic laser scattering (DLS). The micelles could be cross-linked using a catalytic amount of dithiothreitol (DTT). Compared with the non-cross-linked micellar nanoparticles (NPs) (NCL-NPs), the cross-linked NPs (CL-NPs) were more stable against dilution and high salt concentration. However, in the presence of 10 mM glutathione (GSH), the reducible CL-NPs swelled in a short time, as determined by DLS. In in vitro drug release experiments, the CL-NPs exhibited pH and reduction dual responsive DOX release behaviour. DOX was released slowly under physiological pH (7.4) and/or in the absence of reduced GSH, but was released quickly at endosomal pH (5.5) and in the presence of GSH (5 mM or 10 mM). Both the confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) studies indicated that CL-NPs could be effectively taken up by the HeLa cells, and the conjugated DOX was efficiently released in response to the intracellular environment (low pH and high GSH concentration). In the MTT assay, CL-NPs could effectively inhibit the proliferation of HeLa cells and A549 cells. Thus, CL-NPs with superior stability, pH and reduction dual responsive drug release behaviour and good anti-cancer capability showed promise for anti-cancer treatment in the future.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yalin Wu,Qingxiang Zhou,Yongyong Yuan,Hongyuan Wang,Yayan Tong,Yali Zhan,Xueying Sheng,Yi Sun,Xianqi Zhou
Phthalate esters (PAEs) are an important kind of environmental endocrine disrupting chemicals , and have attracted great attention in environmental field. Present study described a new method for rapid and sensitive determination of PAEs including dibenzyl phthalate (DPhP), dibutyl phthalate (DnPP), and dicyclohexyl phthalate (DCHP) from aqueous matrices based on magnetic solid-phase extraction. Polyamidoamine (PAMAM) dendrimers-grafted magnetic-nanoparticles were synthesized and characterized, and the expected integration of more multifunctional sites of PAMAM dendrimers and rapid separation property was utilized for method development. To achieve the best extraction efficiency, several important parameters were optimized including the dosage of the adsorbent, sample pH, kind and volume of eluent , extraction time, desorption time, ionic strength . Under the optimal conditions, three phthalate esters were well enriched and simultaneously determined by high performance liquid chromatography with variable wavelength detector (VWD). Excellent linearities were observed in the range of 0.1–600 μg L −1 for DPhP and DnPP and 0.5–600 μg L −1 for DCHP, and all correlation coefficients (R 2 ) were larger than 0.997. The limits of detection (LODs, S/N = 3) were ranged from 0.025 to 0.16 μg L −1 . The spiked recoveries of PAEs in real water samples were in the range of 93.5–101.8% with satisfied relative standard deviations (RSDs) ranging from 0.9 to 4.1%. The prepared magnetic materials have shown good adsorption capability for PAEs and the developed method earned merits such as high sensitivity, simplicity, rapidness and environmental friendliness , which can be used as a robust alternative tool for monitoring PAEs in water samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zikang Qin,Yulei Ma,Wentao Du,Jing Wei,Jia Song,Xinyue Fan,Lu Yao,Lin Yang,Yuanfa Zhuang,Wenju Jiang,Zhongde Dai
Amine-functionalized Metal-Organic Frameworks (MOFs) stand out as highly promising materials in membrane separation technology. Nevertheless, the incorporation of amine-functionalized additives may lead to uncontrollable alterations in MOF morphology. Two intact blade-like MOFs (PEI (Polyethylenimine)-ZIF-L and PAMAM (Polyamidoamine)-ZIF-L) were successfully synthesized by controlling the conditions and then introduced into Pebax 2533 to fabricate Mixed Matrix Membranes (MMMs). Hydrogen bonding and other interactions with Pebax can be formed by PEI-ZIF-L and PAMAM-ZIF-L to enhance interfacial compatibility. Simultaneously, a reversible reaction with CO 2 molecules can be undergone by the amine groups, resulting in facilitated transport in MMMs. The CO 2 separation performance of the MMMs is enhanced by this process, with the facilitation being more pronounced under humid conditions. Under humid conditions, the CO 2 permeabilities of Pebax-PEI-ZIF-L (2 wt%) and Pebax-PAMAM-ZIF-L (2 wt%) MMMs were 639.4 Barrer and 702.7 Barrer, respectively, while the CO 2 /N 2 selectivities were 26.68 and 24.35, respectively. Both permeability and selectivity exhibited a significant increase of 200% and 130%, respectively, compared to pure Pebax. Furthermore, the MMMs demonstrated excellent long-term stability during 72 h of continuous operation in a humid environment, showcasing significant potential for industrial applications, particularly in humid flue gas carbon capture.
研究领域:
膜科学
·
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）