JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Shuqi Guo,Qiang Zhao,Hongbo Hu,Wei Wang,Muhammad Bilal,Qiang Fei,Xuehong Zhang
Phenazine-1-carboxylic acid (PCA) secreted by Pseudomonas chlororaphis has been commercialized and widely employed as an antifungal pesticide. However, it displays potential hazards to nontarget microorganisms and the environment. Although the PCA degradation characteristics have received extensive attention, the biodegradation efficiency is still insufficient to address the environmental risks. In this study, an engineered Pseudomonas capable of degrading PCA was constructed by introducing heterologous PCA 1,2-dioxygenase (PcaA1A2A3A4). By integrating the PCA degradation module in the chemical mutagenesis mutant P3, 7.94 g/L PCA can be degraded in 60 h, which exhibited the highest PCA degradation efficiency to date and was 35.4-fold higher than that of the PCA natural degraders. Additionally, PCA was converted to 1-methoxyphenazine through structure modification by introducing the functional enzymes PhzSPa and PhzMLa, which has good antifungal activity and environmental compatibility. This work demonstrates new possibilities for developing PCA-derived biopesticides and enables targeted control of the impact of PCA in diverse environments.
研究领域:
环境科学
·
微生物学
·
生物技术
·
化学工程
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Pengfei Bi,Wangsheng Sun,Shiqi Li,Xu Liu,Yuhan Tian,Fangyu Long,Zhe Zhang,Jing Guo
To create the low-alcoholic beverages with excellent nutritional properties and flavor qualities, pomegranate juice was fermented with 16 strains of non- Saccharomyces yeasts from 9 species without added sugar. The effects of different yeasts on the non-volatile constituents (vitamins, organic acids, polyphenols), volatile compounds and sensory characteristics of the fermented beverages were comprehensively evaluated. The study revealed that non- Saccharomyces fermentations resulted in a higher abundance of polyphenols compared to the pomegranate juice and S. cerevisiae WLS21 (Sc21). The monomer phenols in Meyerozyma guilliermondii 12 (Mg12) were approximately twice the total of CK and 1.3 times of Sc21. Moreover, they led to a higher concentration of volatile compounds than the pomegranate juice. A total of 94 volatile compounds were identified in the pomegranate fermented beverages. In contrast to S. cerevisiae , Wickerhamomyces anomalus 1-1 and 10 (Wa1-1, Wa10), Pichia kudriavzevii 8 (Pk8) and Mg12 resulted in more acetate esters, and Zygosaccharomyces rouxii IFO 1130 and 53 (Zr1130, Zr53) yield higher levels of ethyl esters. A total of 40 volatiles with a relative odour activity value (rOAV) of ≥ 0.1 were identified, of which 14 were selected for principal component analysis (PCA). The sensory analysis confirmed that Zr1130, Zr53, Wa1-1, Wa10 and Pk8 strains exhibited superior aroma generation compared to Sc21. Furthermore, the partial least-squares regression (PLSR) analysis revealed that cherry, red fruits, black currant, citrus fruits, raspberry, black berry, pineapple, floral, sour and alcoholic odours had a significantly positive impact on the overall acceptability of the fermented beverages.
研究领域:
食品科学
·
微生物学
·
饮料技术
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Jingna Yan,Anxia Lu,Jirui Kun,Bei Wang,Yiwen Miao,Yingjuan Chen,Chi-Tang Ho,Qing Meng,Huarong Tong
Tea infected with bird’s eye spot disease generally imparts a long-lasting bitter taste, which is unacceptable to most consumers. This study has comprehensively evaluated the taste profiles of infected and healthy teas and investigated their known bitter compounds previously reported in tea. Quantification analyses and calculation of dose-over-threshold (DoT) factors revealed that no obvious difference was visualized in catechins, caffeine, bitter amino acids, and flavonols and their glycosides between infected and healthy tea samples, which was also verified by principal component analysis (PCA) and hierarchical cluster analysis (HCA). Therefore, these known bitter compounds have been ruled out as critical contributors to the long-lasting bitterness of infected teas. Furthermore, Gel permeation chromatography, sensory analysis, and UPLC-Q-TOF-MS were employed and identified 13 substances from the target bitter fractions, including caffeine, ten triterpenoids, and two oxylipins. The higher triterpenoid levels were supposed to be the reason causing the long-lasting bitterness. This study has provided a research direction for the molecular basis of the long-lasting bitterness of infected tea leaves with bird’s eye spot disease.
研究领域:
食品科学
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化学
·
植物病理学
·
感官科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Zhao Zhihao,Ge Yinghao,Xu Lingyun,Sun Xiaohan,Zuo Jing,Wang Zhenglin,Liu Hongyang,Jiang Xiangyu,Wang Dong
Organic vapor sensors based on polymer owing to their tunable molecular structures and designable functions have attracted considerable research interest. However, detecting multiple organic vapors with high accuracy and a low detection limit is still challenging. Herein, inspired by the mammalian olfactory recognition system, organic vapor sensors based on one-dimensional microfilament array structures with a wide range of sensing gases are demonstrated. By introducing aggregation-induced emission (AIE) molecules, sensors possess dual-optical sensing mechanisms of variation in fluorescence intensity and wavelength. By virtue of the synergistic effects of dual signals, superb accuracy and incredibly low detection limit are achieved for identifying analytes. In particular, the polymer/AIE microfilament array can detect acetone vapor down to 0.03% of saturated vapor pressure. In the saturated vapor of acetone, the fluorescence intensity of the sensor arrays was reduced by 53.7%, while the fluorescence wavelength was red-shifted by 21 nm. Combined with the principal component analysis (PCA) algorithm, the polymer/AIE molecular sensor arrays accomplished the classification and identification of acetone, ethanol, methylene chloride, toluene, and benzene. This bioinspired approach with dual sensing signals may broaden practical applications to high-performance gas sensors for precise molecular detection.
研究领域:
材料科学
·
化学传感器
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分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Xiong Xiang,Amakye William Kwame,Yinglu Qing,Shan Li,Min Wang,Jiaoyan Ren
Effect of antioxidants in oxidative stress-induced cell damage has been extensively studied, but its effects on cell macroscopic phenomena have rarely been explored. Cell morphology and motility are two interrelated cellular processes reflecting cellular function phenotype, and the real-time imaging technology is key for the study of these processes. In this study, we used two known antioxidants, pyrroloquinoline quinone (PQQ) and rutin , to explore the antioxidant effects on cell morphology and motility in rotenone-damaged NIH3T3 cells, as well as roles in their antioxidant function based on Cytation 7 Cell Imaging Multi-Mode Reader. First, NIH-3T3 cells were pretreated with PQQ and rutin for 4 h and then rotenone was used to induce oxidative damage. It was found that PQQ (2 μM) and rutin (300 μM) had similar protective effects on the proliferation activity of rotenone-damaged cells. Subsequently, the morphology and motility of the treated cells were quantified. Results showed that both PQQ and rutin could significantly inhibit morphological changes including cell shape index and cell surface of the damaged cells, however PQQ had a stronger effect on cell motility under the specific experimental concentrations. Using principal component analysis (PCA), we further demonstrated that the quantified cell morphology and motility were consistent with the cell proliferation activity. Consequently, quantification of cell morphology and motility as evaluation indices may serve as potential reference values for the evaluation of biological activity of antioxidants.
研究领域:
细胞生物学
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药理学
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生物医学工程
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Houyang Ge,Qizhen Chen,Sheng Chen,Cong Li,Xingen Gao,Wanqiang Fu,Hongyi Zhang,Fuqiang Wang,Xianqiong Gong,Junzheng Wu,Juqiang Lin
Plasma-free amino acid (PFAA) profiling is crucial for monitoring and evaluating cancer progression. However, there remains a lack of sensitive, simple, and direct methods for rapid PFAA detection. In this study, we developed a vapor-deposited 3D flexible porous structure named FPS Ag - Coated serving as a high-performance surface-enhanced Raman spectroscopy (SERS) platform for ultrasensitive detection of micro PFAAs (10 −4 M). The surface of the platform contains abundant hydroxyl functional groups, which not only confer exceptional flexibility but also significantly minimize background signal interference. The 3D FPS architecture provides densely packed hotspots for Raman signal enhancement while demonstrating excellent cost-effectiveness and scalability for mass production. We systematically evaluated the platform's detection capability using four representative amino acids: fluorescent amino acids (L-tyrosine and L-tryptophan), weakly fluorescent amino acid (L-phenylalanine), and non-fluorescent amino acid (L-serine). Across a concentration range of 10 −1 to 10 −4 M, the platform effectively measured characteristic SERS signals with linear correlation, enabling real-time monitoring and quantitative analysis of PFAAs. When applied to clinical plasma samples from cancer patients, the platform successfully identified PFAAs-specific SERS signal. Subsequent principal component analysis (PCA) achieved discrimination between different cancer types and healthy volunteers with an accuracy exceeding 83.3 %. This work presents a high-performance SERS platform for PFAA recognition, offering a highly adaptable flexible material that could facilitate the clinical translation of SERS-based diagnostics.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
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纳米技术
·
材料科学
·
光谱学
·
临床医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Qian Liu,Xiaoying Yang,Longhui Luo,Wei Tian,Chao Kang,Tao Sun,Yuegang Chen,Juan Tao,Dongmei Chen
Benzimidazole fungicides are widely used in agriculture, and residues in food will threaten public health and safety. In this study, MXene@PEI@AgNPs composites were successfully constructed by electrostatic self-assembly method using polyethyleneimine (PEI) as a linker and used as a surface-enhanced Raman scattering (SERS) active substrate. The material combines the electromagnetic enhancement of silver nanoparticles and the chemical enhancement mechanism of MXene, showing excellent SERS performance. The experimental results show that the SERS substrate has excellent enhancement performance and successfully obtains the characteristic spectra of six target fungicides. After that, it was combined with chemometrics methods, through principal component analysis (PCA), it was found that the SERS spectra of different fungicides showed obvious clustering distribution. The constructed support vector machine (SVM) classification model shows excellent prediction performance, with an accuracy rate of 100 %. In terms of quantitative analysis, the method showed good sensitivity, and the detection recoveries of benzimidazole fungicides in actual samples such as kiwifruit, tomato and citrus were 81.62 % −113.87 %. Finally, in order to verify the reliability of the method, the high performance liquid chromatography (HPLC) method was used for comparative verification. The results showed that the two methods had good consistency.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
光谱学
·
农业科学
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产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2020
·Vol. 14
·期 4
·2010-2020
·
IF 1.648
Huang Shuai,Wang Qiang,Wang Yaosong,Ying Ruifeng,Fan Gongjian,Huang Meigui,Agyemang Mary
This study characterized the quality, composition and antioxidant activities of olive oil from four olive varieties grown in Chongqing, southwest of China. With the fruit skin turned from green to semi black, the percentage of oleic acid decreased while the percentage of linoleic acid increased. The highest concentrations of total phenolic content, total flavonoid content and total antioxidant activity were found in the Coratina olive oils, in which the lowest content of oleuropein was also observed. Significant differences of fatty acid composition at sn-2 position were observed among the varieties, with the percentage of oleic acid at sn-2 position in Picual olive oils were significantly higher than that of other varieties ( p < 0.05). In addition, the different varieties of olive oil samples were divided into three categories by principal component analysis (PCA), and the quality of Coratina olive oils were greatly affected by fruit ripening stage. With the fruit ripening, the levels of hydroxytyrosol, rutin, total phenolics, total flavonoids and antioxidant activities in Coratina olive oil all declined. This work can provide guidance for the harvesting time and processing of olives in Chongqing.
研究领域:
农业
·
食品科学
·
化学
·
营养学
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产品关联
DOI
高影响因子
Food Science & Nutrition
·
2020
·Vol. 8
·期 6
·2882-2894
·
IF 1.797
Changyuan Wang,Yuchao Feng,Shu Zhang,Tianxin Fu,Yanan Sheng,Yiwei Zhang,Yingjun Jiang,Miao Yu,Liyuan Zhang,Dongjie Zhang
Metabolomic studies were carried out using gas chromatography and mass spectrometry (GC-MS) on Daohuaxiang variety rice ( Oryza sativa L.) from the Wuchang Geographical Indication Rice Protection Area in Heilongjiang Province, to investigate the effects of storage on brown rice metabolism. The data were subjected to principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and cluster analysis using software such as SIMCA. Analysis of the samples led to the identification of a total of 160 metabolites. No significant differences were found in the amount of metabolites before and after storage. A total of 31 differential metabolites were screened, and the changes in metabolite content showed a “reverse change” overall. Storage significantly changed the content of various metabolites in rice, with fatty acids impacted most significantly. Metabolic pathway analysis revealed that fatty acid biosynthesis is a key metabolic pathway in rice storage. The degradation of brown rice quality caused by storage is closely related to the composition and content of its metabolites, and that change in lipid content significantly affects brown rice quality during storage.
研究领域:
农业科学
·
食品科学
·
分析化学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuan Pan,Hui-Wen Gu,Yi Lv,Xiao-Li Yin,Ying Chen,Wanjun Long,Haiyan Fu,Yuanbin She
Identifying geographical origins of red wines made in specific regions is of significance since the false claim of geographical origins has been frequently exposed in China's wine industry. In this work, an untargeted metabolomic approach based on UPLC-QTOF-MS was established to discriminate geographical origins of Chinese red wines. The principal component analysis (PCA) showed significant differences between wine samples from three famous geographical origins in China. The metabolites contributing to the differentiation were screened by orthogonal partial least squares-discriminant analysis (OPLS-DA) with pairwise modeling. 40 and 46 differential metabolites in positive and negative ionization modes were putatively identified as chemical markers. Furthermore, heatmap visualization and OPLS-DA models were constructed based on these identified markers and external verification wine samples from different regions were successfully discriminated, with recognition rate up to 96.7%. This study indicated that UPLC-QTOF-MS-based untargeted metabolomics has great potential for the geographical origin traceability of Chinese red wines.
研究领域:
食品科学
·
分析化学
·
代谢组学
·
葡萄酒科学
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产品关联
DOI
PubMed
高影响因子
Molecular Cancer
·
2022
·Vol. 21
·期 1
·1-23
·
IF 41.444
Yao Bing,Zhu Sha,Wei Xiyi,Chen Ming-Kun,Feng Yangkun,Li Zhimin,Xu Xinyu,Zhang Yuwei,Wang Yang,Zhou Jingwan,Tang Ningyuan,Ji Chengjian,Jiang Peng,Zhao Shan-Chao,Qin Chao,Feng Ninghan
Background Prostate cancer (PCa) is the most frequently diagnosed malignancy in men, and its mechanism remains poorly understood. Therefore, it is urgent to discover potential novel diagnostic biomarkers and therapeutic targets that can potentially facilitate the development of efficient anticancer strategies.Methods A series of functional in vitro and in vivo experiments were conducted to evaluate the biological behaviors of PCa cells. RNA pulldown, Western blot, luciferase reporter, immunohistochemistry and chromatin immunoprecipitation assays were applied to dissect the detailed underlying mechanisms. High-throughput sequencing was performed to screen for differentially expressed circRNAs in PCa and adjacent normal tissues.Results Upregulation of protein arginine methyltransferase 5 (PRMT5) is associated with poor progression-free survival and the activation of multiple signaling pathways in PCa. PRMT5 inhibits the transcription of CAMK2N1 by depositing the repressive histone marks H4R3me2s and H3R8me2s on the proximal promoter region of CAMK2N1, and results in malignant progression of PCa both in vitro and in vivo. Moreover, the expression of circSPON2, a candidate circRNA in PCa tissues identified by RNA-seq, was found to be associated with poor clinical outcomes in PCa patients. Further results showed that circSPON2 induced PCa cell proliferation and migration, and that the circSPON2-induced effects were counteracted by miR-331-3p. Particularly, circSPON2 acted as a competitive endogenous RNA (ceRNA) of miR-331-3p to attenuate the repressive effects of miR-331-3p on its downstream target PRMT5.Conclusion sOur findings showed that the epigenetic regulator PRMT5 aggravates PCa progression by inhibiting the transcription of CAMK2N1 and is modulated by the circSPON2/miR-331-3p axis, which may serve as a potential therapeutic target for patients with aggressive PCa.
研究领域:
肿瘤学
·
分子生物学
·
表观遗传学
·
癌症生物学
·
分子遗传学
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Yanhua Pan,Yini Ma,Jiadong He,Lu Zhang,Rong Huang,Ling Tang,Jiaxuan Ren,Weicheng Wang,Muriel Quinet,Yu Meng
This study addresses the identification and comparison of volatile organic compounds (VOCs) in different peony varieties to elucidate their distinct aroma profiles and the common VOCs that constitute the unique fragrance of peony flowers, laying a foundation for breeding high-quality peony varieties and developing related products. Headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) was employed to analyze and differentiate the VOCs of eight peony varieties: TAOXI, ZIHELIAN, BAITIANE, YAOHUANG, DAFUGUI, DAOJIN, HONGXIUQIU, and MANAOPAN. The study identified 87 VOCs, revealing significant differences in VOC compositions among the samples. Notably, BAITIANE displayed distinct differences in both color and VOC composition compared to the other seven varieties. It exhibited uniquely high levels of butanoic acid-M, butanoic acid-D, hexanal-D, ethanol-D, and ethyl acetate. Twelve key differential VOCs were identified as having significant impacts: ethanol-D, hexanal-D, (e)-2-hexenal-D, (e)-2-hexenal-M, hexanal-M, butanoic acid-M, acetic acid-D, acetic acid-M, butanoic acid-D, ethyl acetate, thiophene, and dimethyl sulfide. KEGG pathway analysis highlighted the roles of xenobiotics biodegradation, terpenoid metabolism, and amino acid metabolism in VOC biosynthesis. The findings are significant for the breeding of fragrant peony varieties and the development of floral-based products. The findings provide theoretical support for breeding new horticultural flower varieties and developing commercial peony products, enhancing the understanding of floral scent chemistry, and offering valuable information for the perfumery, floral food processing, and related industries.
研究领域:
植物学
·
香味化学
·
植物育种
·
香料工业
·
食品加工
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）