TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2025
·
IF 3.4
Simiao Qiao,Fanshu Li,Jia Huang,Xiaoqian Wu,Xiaoying Cao,Xifeng Zhang,Zhifeng Wei
This study was designed to clarify the reason for PM 2.5 to induce the ferroptosis of bronchial epithelial cells and worsen asthma in detail, advancing the development of relevant drugs. Results displayed that PM 2.5 mainly induced ferroptosis but not apoptosis of bronchial epithelial cells, indicating by the changes of lipid reactive oxygen species (ROS), iron, malondialdehyde, lactic dehydrogenase, nuclear receptor coactivator 4, glutathione and glutathione peroxidase 4 levels, and further confirmed by using in combination with the ferroptosis and apoptosis inhibitors. It also caused the mitochondrial disturbance, reduced the level of mitochondrial membrane potential, up-regulated the level of mitochondrial ROS, and mechanisms were subsequently ascribed to dynamin-related protein 1 (DRP1)-related mitochondrial fission. Furthermore, the up-regulation on ubiquitination of pyruvate kinase M2 (PKM2) but not macrophage stimulating 1 or interferon regulatory factor 1, the transcriptional regulators locating at the upstream of DRP1, was showed by PM 2.5 via controlling “aryl hydrocarbon receptor (AHR)-heat shock protein 90 (HSP90)” axis. In addition, isoliquiritigenin (ISL), the main active ingredients in licorice, was demonstrated to prevent the PM 2.5 -induced activation of AHR-PKM2-ferroptosis in asthma. In conclusion, PM 2.5 activated “AHR-HSP90” axis to disturb DRP1-related mitochondrial fission, induced ferroptosis of bronchial epithelial cells, and worsened asthma. Conversely, ISL restored the above-mentioned signals, ferroptosis and asthma mediated by PM 2.5 , and was suggested as a potential compound to combat the exacerbation of asthma.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Peiru Luo,Mengke Bai,Qingyun He,Zifang Peng,Lingyun Wang,Chuan Dong,Zenghua Qi,Wenfen Zhang,Yanhao Zhang,Zongwei Cai
The broad application of plastic products has resulted in a considerable release of microplastics (MPs) into the ecosystem. While MPs in other environmental matrices (e.g., soil and water) have been studied for a long time, the atmospheric fine particulate matter (PM2.5)-bound MPs are rarely investigated due to the lack of an appropriate analytical approach. The prevalently used visual and spectroscopic means (e.g., optical microscopy, Fourier-transform infrared spectroscopy, and Raman spectroscopy) suffer from obvious drawbacks that cannot precisely detect MPs of tiny sizes and provide quantitative information. In the present study, a novel strategy that does not require sample pretreatment was developed to first effectuate accurate quantification of polyethylene MP (PE-MP) in PM2.5 based on pyrolysis-gas chromatography–tandem mass spectrometry (Pyr-GC-MS/MS). It featured acceptable recoveries (97%–110%), high sensitivity (LOD = 1 pg), and qualified precisions (RSD of 3%–13%). Employing this approach, for the first time, exact atmospheric concentrations of PE-MPs in PM2.5 from megacities in North (Zhengzhou and Taiyuan) and South (Guangzhou) China were obtained, and relatively serious pollution was found in Taiyuan. The 100% sample detection rates also suggested the widespread occurrence and possible human exposure risks of PM2.5-bound PE-MPs. In brief, the new strategy could conduct direct, sensitive, and accurate quantification of PE-MP in PM2.5, favoring further studies of environmental fates, distributions, and toxicities of atmospheric MPs.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Weiru Wang,Shizhen Zhao,Lele Tian,Shaojun Lv,Jun Li,Zhen Zhao,Duohong Chen,Ping’an Peng,Gan Zhang
Per- and polyfluoroalkyl substances (PFAS) are global pollutants of concern, yet their atmospheric sources and secondary formation mechanisms remain poorly understood. Here, we collected PM 2.5 samples during winter and summer across nine cities in the Pearl River Delta (southern China). Among 44 targeted PFAS in PM 2.5 , 15 were detected with a median concentration of 222 pg/m 3 , with ultra-short-chain PFAS accounting for 42% of the total concentration. Higher Σ 15 PFAS concentrations occurred in winter (261 pg/m³) than in summer (141 pg/m³), with distinct seasonal compositional patterns. Source apportionment analysis indicated that potential secondary formation contributed an average of 16% to Σ 15 PFAS. Significant correlations between selected perfluoroalkyl acids (PFAAs) and atmospheric oxidants, aerosol liquid water content, and succinic acid point to aqueous-phase precursor degradation as a potential pathway for PFAS formation. These results suggest that PM 2.5 may act as a sink for PFAS, especially for ultrashort-chain species during winter. Overall, our study provides field-based evidence for potential secondary PFAS formation in ambient aerosols and underscores the importance of controlling precursor emissions to mitigate atmospheric PFAS pollution.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2025
·
IF 5.9
You Zhou,Kai Yang,Jian Wang,Ruijuan Guan,Wei Liu,Chaowei Wen,Liang Yuan,Tiange Zhang,Wei Feng,Yuanyuan Li,Jingyi Xu,Wenju Lu
Backgrounds: Exposure to fine particulate matter (PM2.5) triggers airway inflammation through the activation of the Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway, contributing to the pathogenesis of various acute and chronic respiratory diseases. Mucin 1 (MUC1), a transmembrane glycoprotein highly expressed on airway epithelial cells, is known to modulate TLR-mediated inflammatory responses. However, the specific role and molecular mechanism by which MUC1 regulates PM2.5-induced airway inflammation remain inadequately understood.Methods: Lung injury models were established using Muc1+/+ and Muc1-/- rats via intranasal instillation of PM2.5. Histopathological changes and inflammatory responses were evaluated following treatment. In parallel, human bronchial epithelial cells (16HBE) were transfected with MUC1 overexpression and knockdown plasmids, along with corresponding controls. An IRAK4-specific inhibitor was employed to explore the mechanistic role of MUC1 in regulating the TLR4/IRAK4/NF-κB signaling cascade and associated pyroptosis.Results: PM2.5 exposure caused notable epithelial disruption, inflammatory infiltration, and submucosal fibrosis in Muc1-/- rats compared to Muc1+/+ controls. While TLR4 expression was not significantly altered, NF-κB and NLRP3 inflammasome activity were markedly elevated in Muc1-deficient rats. In 16HBE cells, MUC1 overexpression attenuated, whereas MUC1 knockdown exacerbated, PM2.5-induced activation of the IRAK4/NF-κB signaling axis and pyroptosis-related markers (IL-1β, IL-18, NLRP3, GSDMD). Furthermore, pharmacological inhibition of IRAK4 mitigated these effects, confirming the involvement of the IRAK4 pathway in MUC1-mediated protection.Conclusions: MUC1 is upregulated in airway epithelial cells upon PM2.5 exposure and serves as a key endogenous inhibitor of airway inflammation. It exerts its anti-inflammatory and anti-pyroptotic effects by suppressing IRAK4 phosphorylation, thereby modulating downstream NF-κB/NLRP3/GSDMD signaling. These findings highlight MUC1 as a potential therapeutic target for PM2.5-induced airway inflammatory diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Yang Ming,Xiaonan Zhou,Gang Liu,Mieradilijiang Abudupataer,Shichao Zhu,Bitao Xiang,Xiujie Yin,Hao Lai,Yongxin Sun,Chunsheng Wang,Jun Li,Kai Zhu
Air pollution is a major public health concern worldwide. Exposure to fine particulate matter (PM2.5) is closely associated with cardiovascular diseases. However, the effect of PM2.5 exposure on thoracic aortic aneurysm and dissection (TAAD) has not been fully elucidated. Diesel exhaust particulate (DEP) is an important component of PM2.5, which causes health effects and is closely related to the incidence of cardiovascular disease. In the current study, we found that DEP exposure increased the incidence of aortic dissection (AD) in β-aminopropionitrile (BAPN)-induced thoracic aortic aneurysm (TAA). In addition, exposure to PM2.5 increased the diameter of the thoracic aorta in mice models. The number of apoptotic cells increased in the aortic wall of PM2.5-treated mice, as did the protein expression level of BAX/Bcl2 and cleaved caspase3/caspase3. Using a rhythmically stretching aortic mechanical simulation model, fluorescent staining indicated that PM2.5 administration could induce mitochondrial dysfunction and increase reactive oxygen species (ROS) levels in human aortic smooth muscle cells (HASMCs). Furthermore, ERK1/2 mitogen-activated protein kinase (MAPK) signaling pathways participated in the apoptosis of HASMCs after PM2.5 exposure. Therefore, we concluded that PM2.5 exposure could exacerbate the progression of TAAD, which could be induced by the increased apoptosis in HASMCs through the ERK1/2 MAPK signaling pathway.
研究领域:
心血管疾病
·
环境健康
·
分子生物学
·
病理生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Zhaoxiang Zhong,Zhe Xu,Ting Sheng,Jianfeng Yao,Weihong Xing,Yong Wang
Porous membranes/filters that can remove airborne fine particulates, for example, PM2.5, with high efficiency at low energy consumption are of significant interest. Herein, we report on the fabrication of a new class of unusual superior air filters with ultrahigh efficiency and an interesting antibacterial functionality. We use atomic layer deposition (ALD) to uniformly seed ZnO on the surface of expanded polytetrafluoroethylene (ePTFE) matrix, and then synthesize well-aligned ZnO nanorods with tunable widths and lengths from the seeds under hydrothermal conditions. The presence of ZnO nanorods reduces the effective pore sizes of the ePTFE filters at little expense of energy consumption. As a consequence, the filters exhibit exceptional dust removal efficiencies greater than 99.9999% with much lower energy consumption than conventional filters. Significantly, the presence of ZnO nanorods strongly inhibits the propagation of both Gram positive and negative bacteria on the filters. Therefore, the functionalized filters can potentially overcome the inherent limitation in the trade-off effect and imply their superiority for controlling indoor air quality.
研究领域:
材料科学
·
环境工程
·
纳米技术
·
生物医学工程
·
空气污染控制
✓ 已验证
DOI
高影响因子
ATMOSPHERIC ENVIRONMENT
·
2017
·
IF 3.629
Rui Li,Xinfeng Wang,Rongrong Gu,Chunying Lu,Fanping Zhu,Likun Xue,Huijun Xie,Lin Du,Jianmin Chen,Wenxing Wang
Particulate biogenic organic nitrates (PBONs) are important components of secondary organic aerosols and play an important role in the tropospheric atmosphere chemistry. However, the concentrations and the chemistry of PBONs remain poorly understood due to the lack of accurate measurement techniques on specific organic nitrates. In this study, ultra high performance liquid chromatography/electrospray mass spectrometry was applied in detection of individual PBONs in ambient atmosphere. Total five kinds of PBONs were identified in PM 2.5 samples collected in urban Ji'nan in spring according to characteristic fragments of NO 2 , NO 3 , HNO 3 , CO 2 , and H 2 O, including monoterpene hydroxyl nitrate (MW = 215, MHN215), pinene keto nitrate (MW = 229, PKN229), limonene di-keto nitrate (MW = 247, LDKN247), oleic acid keto nitrate (MW = 359, OAKN359), and oleic acid hydroxyl nitrate (MW = 361, OAHN361). Among them, three kinds of PBONs originated from biogenic volatile organic compounds of pinene and limonene and two kinds of PBONs came from chemical conversion of oleic acid. The concentrations of these PBONs were roughly quantified with surrogate standards of (1R,2R,5R)-(+)-2-hydroxy-3-pinanone and ricinoleic acid. The average concentrations of MHN215, PKN229, LDKN247, OAKN359, and OAHN361 were 111.6 ± 23.0, 93.1 ± 49.6, 55.3 ± 7.4, 23.4 ± 14.5, 36.8 ± 18.3 ng m −3 , respectively. The total concentration of these PBONs was 325.4 ± 116.7 ng m −3 , contributing to 1.64 ± 0.34‰ of PM 2.5 .
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·
IF 12.2
Jianing Liu,Lijin Zeng,Chen Tang,Yuting Shen,Yingjing Miao,Jiewen Huang,Ting Fang
Bulk PM2.5 measurements are widely used to assess the oxidative toxicity of particulate matter, but they integrate size fractions that differ in composition and respiratory deposition. We show that mixing PM1 and PM1-2.5 from a roadside site produces pronounced antagonistic effects on oxidative potential (OP) and reactive oxygen species (ROS) generation, with interaction factors (IFs) below 1 across all end points: OP by the ascorbic acid assay (IF = 0.65-0.81), OP by the dithiothreitol assay (IF = 0.80-0.93), ROS (IF = 0.10-0.88), and mitochondrial superoxide (IF = 0.43-0.78). This antagonism requires metal-organic interactions as metal-free biomass burning PM exhibits strictly additive behaviors. Cu2+-humic acid experiments reproduce the antagonistic effects seen in ambient PM (IFs = 0.22-0.95). Electron paramagnetic resonance spectroscopy confirms the formation of complexed Cu and reveals that the conversion of free Cu to complexed Cu is nonlinear with respect to the HA:Cu ratio, providing a mechanistic explanation for the divergent interaction effects reported across studies. These results indicate that bulk PM2.5 measurements can mask the oxidative toxicity of metal-organic interactions, highlighting the need for size-resolved OP and ROS characterization.
✓ 已验证
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
JingHong Li,Nan Chen,YiPing Tian,Hui Xu
The exploration of volatile organic compounds (VOCs) produced by cell lines may be a powerful and non-invasive tool for the study of the health risk of human exposure to atmospheric particulate matter (PM). In this work, we developed a sensitive solid phase microextraction-gas chromatography-mass spectrometry method (SPME-GC-MS) to analyze VOCs in breathed gas of PM2.5-induced human embryonic fibroblast cell line (MRC-5). A novel graphene oxide/polyaniline/polydopamine (GO/PANI/PDA) coating was prepared on a stainless steel wire via electrochemical deposition and self-polymerization for the first time. The GO/PANI/PDA coating exhibited high extraction efficiency, good thermal stability (> 380 ℃), excellent mechanical stability as well as long service time (> 150 times). Parameters that may affect the results were optimized systematically. Under the optimal conditions, VOCs including benzene series, aldehydes and alkane were detected with low limit of detection (0.2–2.0 μg L −1 ) and good correlation (correlation coefficients above 0.9922). The relative standard deviations of within-day and between-day were 1.1–8.4% and 0.2–11.2%, respectively. Satisfactory recoveries of 82–117% indicated good repeatability of the method. The method has been successfully applied for the determination of target VOCs in the headspace gas of PM2.5-induced MRC-5 cell. And it is expected to provide an alternative tool for the study of cytotoxicology of atmospheric particulates.
研究领域:
环境生物学
·
毒理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 25
·2531-2540
·
IF 3.532
Wenjing Li,Maoyu Cao,Pengxiang Ge,Xiaoyu Fu,Jiajie Tang,Mindong Chen
Organosulfates (OSs) derived from the oxidation of biogenic volatile organic compounds (BVOCs) in the presence of anthropogenic sulfate aerosols are the important tracers of secondary organic aerosols (SOAs). In order to better understand the concentration of pinene-nitrooxy organosulfates (pNOSs) in Nanjing, a sensitive high-performance liquid chromatography-electron spray ionization spectrum/mass spectrum (HPLC-ESI-MS/MS) to determine pNOSs in PM2.5 has been developed and validated in this study. Firstly, Hypersil Gold C18 (Thermo Scientific, San Jose, USA) was selected to separate pinene-derived nitrooxy organosulfates (pNOSs) based on their polarity. Three kinds of pNOSs were detected in the full scan mode (MS) with an ESI source under the negative mode. Secondly, three isomers of pNOSs with fragment ions m/z 220, 151, and 142 were identified based on the MS/MS maps. At least two pairs of transfer ions should be selected as identification and quantification ions according to the optimization results of target compounds. For example, to determine pNOSs, these transfer ions of m/z 294 → 247, m/z 294 → 231, m/z 294 → 220, m/z 294 → 142, m/z 294 → 151, m/z 294 → 96, m/z 294 → 80 were selected as quantification and identification ions. Finally, the influence of scan mode on pNOS detection was evaluated, and the results showed that pNOSs were most sensitive in the SRM (selected reaction monitor) scan mode. Therefore, the SRM scan mode was chosen to detect pNOSs. We applied this method to analyze year-round PM2.5 (PM2.5 is fine particulate matter, which refers to particulate matter in ambient air with an aerodynamic equivalent diameter of less than or equal to 2.5 microns) samples in Nanjing. The average concentration of all the three kinds of pNOSs was 69.95 ng m−3. The results showed that the average concentration of pNOSs was high in spring (92.94 ng m−3) and summer (90.57 ng m−3), and lowest in winter (30.03 ng m−3).
研究领域:
大气化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2020
·
IF 7.864
Min Chen,Xiaoliang Liao,Shi-chao Yan,Yanpeng Gao,Chun Yang,Yuanyuan Song,Yi Liu,Weiquan Li,Suk-Ying Tsang,Zhi-Feng Chen,Zenghua Qi,Zongwei Cai
Although the bioaccumulation of organophosphate flame retardants (OPFRs) in aquatic organisms has been investigated, little information is available about their bioaccumulation in mammals following chronic inhalation exposure. To address this knowledge gap, C57BL/6 mice were exposed to 7 PM2.5-associated OPFRs via the trachea to study their bioaccumulation, tissue distribution, and urinary metabolites. Low (corresponding to the real PM2.5 concentrations occurring during winter in Guangzhou), medium, and high dosages were examined. After 72 days’ exposure, ∑OPFR concentrations in tissues from mice in the medium dosage group decreased in the order of intestine > heart > stomach > testis > kidney > spleen > brain > liver > lung > muscle. Of the OPFRs detected in all three exposure groups, chlorinated alkyl OPFRs were most heavily accumulated in mice. We found a significant positive correlation between the bioaccumulation ratio and octanol–air partition coefficient (KOA) in mice tissues for low log KOW OPFR congeners (log KOW ≤ 4, p < 0.05). Three urinary metabolites (di-p-cresyl phosphate: DCrP, diphenyl phosphate: DPhP, dibutyl phosphate: DnBP) were detected from the high dosage group. These results provide important insights into the bioaccumulation potential of OPFRs in mammals and emphasize the health risk of chlorinated alkyl OPFRs.
研究领域:
环境科学
·
毒理学
·
公共卫生
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·
IF 7.5
Enfu Wang,Yu Miao,Weimiao Lu,Chunping Dai,Bin Wu,Wenbiao Zhang
Efficient removal of fine particulate matter (PM2.5) from air and organic pollutants from water remains a critical challenge in the treatment of industrial emissions. Cellulose nanofibril (CNF)-based aerogels are promising media, but conventional hydrophobic modification often relies on fluorosilanes or organic solvents. Here, we developed a reusable, antibacterial CNF-based aerogel through a fully aqueous dual-silane modification strategy incorporating bamboo-activated carbon (BAC)@Ag composite particles. In this system, protonation of the amino groups of 3-(2-aminoethylamino)propyltrimethoxysilane in water generated cationic species that promoted the uniform dispersion and aqueous hydrolysis of octyltrimethoxysilane. The hydrolysed silane chains formed covalent bonds with the hydroxyl groups of CNFs, imparting hydrophobicity (water contact angle of 135.4°), thermal stability, and mechanical resilience (height loss of only 18.8% after 50 compression cycles at 50% strain) to the aerogel, supporting its reusability. BAC provided a porous, negatively charged platform for pollutant capture and supported uniformly dispersed Ag nanoparticles, which produced a bacterial reduction of 99.99% against both Escherichia coli and Staphylococcus aureus . The resulting aerogel achieved a PM2.5 filtration efficiency of 98.62% at a low pressure drop of 37 Pa and absorption capacity of 28.2-55.0 g/g for oils and organic solvents. Multiphysics simulations were performed to examine PM2.5 capture, oil–water separation, and antibacterial kinetics. This work provides an aqueous, fluorine-free strategy for developing multifunctional aerogels.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）