SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Min Xia,Xuedong Wang,Jiaqi Xu,Qiuhui Qian,Ming Gao,Huili Wang
The organophosphate flame retardant, tris (1-chloro-2-propyl) phosphate (TCPP), is ubiquitous in environmental matrices; however, there is a paucity of information concerning its systemic toxicity. Herein, we investigated the effects of TCPP exposure on zebrafish neurodevelopment and swimming behavior to elucidate the underlying molecular mechanisms of neurotoxicity. Under TCPP gradient concentration exposure, the hatching rates were declined by up to 33.3% in 72 hpf, and the malformation rates increased from 15% to 50%. Meanwhile, TCPP led to abnormal behaviors including decreased locomotive activity in the dark and slow/insensitive responses to sound and light stimulation of larvae. TCPP caused excessive apoptosis and ROS accumulation in early embryonic development, with hair cell defects and structural deformity of neuromast. Abnormal expression of neurodevelopment ( pax6a , nova1 , sox11b , syn2a , foxo3a and robo2 ) and apoptosis-related genes ( baxa , bcl2a and casp8 ) revealed molecular mechanisms regarding abnormal behavioral and phenotypic symptoms. Chronic TCPP exposure led to anxiety-like behavior and excessive panic, lower capacity for discrimination and risk avoidance, and conditioned place preference in adults. Social interaction tests demonstrated that long-term TCPP stress resulted in unsociable, eccentric, lonely and silent behaviors in adults. Zebrafish memory and cognitive function were severely reduced as concluded from T-maze tests. Potential mechanisms triggering behavioral abnormality were attributed to histopathological injury of diencephalon, abnormal changes in nerve-related genes at transcription and expression levels, and inhibited activity of AChE by TCPP stress. These findings provide an important reference for risk assessment and early warning to TCPP exposure, and offer insights for prevention/mitigation of pollutant-induced nervous system diseases.
研究领域:
毒理学
·
神经科学
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环境科学
·
发育生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Hang Liu,Hua Yin,Xiaolong Yu,Minghan Zhu,Zhi Dang
In this work, the amino-functionalized metal-organic frameworks (MIL-88B-NH 2 ) was synthesized, characterized and used as heterogeneous photo-Fenton catalyst for tris-(2-chloroisopropyl) phosphate (TCPP) degradation. The photo-Fenton activity of MIL-88B-NH 2 was investigated on the basis of influence factors, such as initial pH and TCPP concentration, and coexisting impurities. The results revealed that MIL-88B-NH 2 +H 2 O 2 +Vis system exhibited a satisfactory degradation efficiency of TCPP (almost 100%) within 60 min accompanied by a good reusability. Noticeably, the degradation kinetics constant of TCPP by MIL-88B-NH 2 +H 2 O 2 +Vis system was 0.086 min −1 , which was visibly higher than that of MIL-88B+H 2 O 2 +Vis system (0.021 min −1 ) since the addition of amino-functionalized organic linker inhibiting the recombination rate of the photo-generated electron-hole pairs and improving the visible light response. Combined with the characterization, the conversion of Fe III to Fe II could be accelerated by the photo-generated electron from the excitation of Fe-O clusters and NH 2 functionalities, which strengthened the decomposition of H 2 O 2 and formed plenty •OH. Simultaneously, six steady products were validated and potential degradation pathways of TCPP were proposed. It was anticipated that MIL-88B-NH 2 could be considered as a desirable and alternative candidate in the application of heterogeneous photo-Fenton reaction to control the environmental risks caused by organophosphate flame retardants (OPFRs).
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·
IF 27.4
Shuoyuan Li,Yan Yue,Wenqi Wang,Mingyue Han,Xufeng Wan,Qiaochu Li,Xiaoting Chen,Jian Cao,Yangming Zhang,Jiyao Li,Jianshu Li,Lei Cheng,Jiaojiao Yang,Duan Wang,Zongke Zhou
Implant-associated infections (IAIs) are the main cause of prosthetic implant failure. Bacterial biofilms prevent antibiotic penetration, and the unique metabolic conditions in hypoxic biofilm microenvironment may limit the efficacy of conventional antibiotic treatment. Escaping survival bacteria may not be continually eradicated, resulting in the recurrence of IAIs. Herein, a sonosensitive metal-organic framework of Cu-TCPP (tetrakis(4-carboxyphenyl) porphyrin) nanosheets and tinidazole doped probiotic-derived membrane vesicles (OMVs) with high-penetration sonodynamic therapy (SDT), bacterial metabolic state interference, and bacterial cuproptosis-like death to eradicate IAIs is proposed. The Cu-TCPP can convert O 2 to toxic 1 O 2 through SDT in the normoxic conditions, enhancing the hypoxic microenvironment and activating the antibacterial activity of tinidazole. The released Cu(II) under ultrasound can be converted to Cu(I) by exogenous poly(tannic acid) (pTA) and endogenous glutathione. The disruption of the bacterial membrane by SDT can enhance the Cu(I) transporter activity. Transcriptomics indicate that the SDT-enhanced Cu(I) overload and hypoxia-activated therapy hinder the tricarboxylic acid cycle (TCA), leading to bacterial cuproptosis-like death. Moreover, the OMVs-activated therapy can polarize macrophages to a M2-like phenotype and facilitate bone repair. The sonodynamic biofilm microenvironment modulation strategy, whereby the hypoxia-enhanced microenvironment is potentiated to synergize SDT with OMVs-activated therapy, provides an effective strategy for antibacterial and osteogenesis performance.
研究领域:
生物材料
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纳米技术
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生物医学工程
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传染病学
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免疫学
·
骨科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yu Wang,Yu Xue,Qilin Zhao,Shuang Wang,Jian Sun,Xiurong Yang
Alzheimer’s disease (AD) is a common chronic neurodegenerative disease that manifests as cognitive impairment and behavioral deficits and severely threatens the health of the elderly. Acetylcholinesterase (AChE) plays a vital role in biological signaling and is an essential target for the early diagnosis and treatment of AD. Herein, 2D Zn-TCPP(Fe) nanosheets (NSs) employing Zn2+ and Fe-bound tetrakis(4-carboxyphenyl)porphyrin ligands were prepared through a surfactant-assisted synthetic method. The ultrathin two-dimensional (2D) metal–organic framework structures exhibited high peroxidase-like activity, which allowed the catalysis of the H2O2-initiated oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB). Such catalytic performance inspired us to develop a convenient, rapid, and sensitive acetylcholinesterase activity assay, during which AChE can catalyze the substrate acetylthiocholine (ATCh) to produce thiocholine (TCh), and TCh could especially enable the degradation of 2D Zn-TCPP(Fe) NSs accompanied by the reduction of ox-TMB production. Our proposed sensing system exhibited favorable selectivity and sensitivity (LOD of 0.029 mU/mL) and has excellent potential to evaluate AChE activity in human serum samples and to screen AChE inhibitors. This colorimetric assay could provide an alternative pathway for early diagnosis and drug screening of AD, facilitating the development of AD therapy.
研究领域:
神经退行性疾病
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生物化学
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纳米技术
·
诊断方法
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药物筛选
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Siqi Wei,Ling Li,Lichen Gou,Li Wu,Xiandeng Hou
Acrylamide (AA) is a carcinogen mainly ingested through food and drinking water, making its accurate determination crucial for both food safety and environmental protection. Herein, we proposed a derivatization-based ratiometric surface-enhanced Raman scattering (SERS) method for the quantification of AA. High density Au NPs were anchored to the surface of Cu-TCPP MOF nanosheets (MOFNs) to form the SERS sensor. The abundant Raman “hot spots” at the nanogaps generated by the Au NPs and the internal standard (IS) signal provided by Cu-TCPP MOFNs improved the sensitivity and quantitative accuracy of the method. Following the thiol-ene click derivatization reaction between p-aminothiophenol (PATP) and AA, the Raman peak intensity ratio (I 1080 /I 395 ) was employed to quantify AA. The linear range was 0.1 nM to 10 μM, and the limit of detection (LOD) was as low as 0.08 nM. Trace amounts of AA in food and water samples were successfully determined using this method.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Jiawei Bao,Jiayu Mi,Yi Xia,Han Gui,Haixue Jia,Dianyu Wang,Hongjing Luo,Linzhu Su,Jiamin Zhang,Jinjian Liu,Jianfeng Liu
The management of multibacterial infections remains clinically challenging in the care and treatment of chronic diabetic wounds. Photodynamic therapy (PDT) offers a promising approach to addressing bacterial infections. However, the limited target specificity and internalization properties of traditional photosensitizers (PSs) toward Gram-negative bacteria pose significant challenges to their antibacterial efficacy. In this study, we designed an iron heme–mimetic PS (MnO2@Fe-TCPP(Zn)) based on the iron dependence of bacteria that can be assimilated by bacteria and retained in different bacteria strains (Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus) and which shows high PDT antibacterial efficacy. For accelerated wound healing after antibacterial treatment, MnO2@Fe-TCPP(Zn) was loaded into a zwitterionic hydrogel with biocompatibility and antifouling properties to form a nanocomposite antibacterial hydrogel (PSB-MnO2@Fe-TCPP(Zn)). In the multibacterial infectious diabetic mouse wound model, the PSB-MnO2@Fe-TCPP(Zn) hydrogel dressing rapidly promoted skin regeneration by effectively inhibiting bacterial infections, eliminating inflammation, and promoting angiogenesis. This study provides an avenue for developing broad-spectrum antibacterial nanomaterials for combating the antibiotic resistance crisis and promoting the healing of complex bacterially infected wounds.
研究领域:
材料科学
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生物医学工程
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药学
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伤口愈合
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传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Jianbo Liu,Jing Liu,Yonghui Shang,Jinqiong Xu,Xinyang Wang,Jianbin Zheng
A multiplex electrochemical immunosensor was developed for the simultaneous detection of two lung cancer markers, carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125), using electroactive probes. Cu-TCPP nanomaterials were synthesized by a hydrothermal method . Cu-TCPP–TB-anti-CEA and Cu-TCPP-PB-anti-CA125 immunosensor probes were prepared using toluidine blue (TB) and prussian blue (PB) as the indicators. The electrochemical immunosensor was constructed for the simultaneous detection of CEA and CA125. The linear range of CEA was 0.1–160 ng mL −1 with a low detection limit of 0.03 ng mL −1 . The linear range of CA125 was 0.5–200 U mL −1 with a low detection limit of 0.05 U mL −1 . Furthermore, the immunosensor was applied for the simultaneous determination of CEA and CA125 in real samples (human blood serum), with results comparable to the reference values obtained by the standard enzyme-linked immunosorbent assay. This study furthers knowledge on the construction of electrochemical immunosensors showing the simultaneous detection of two lung cancer markers.
研究领域:
生物化学
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分析化学
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纳米技术
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癌症研究
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电化学
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Xingyan Liu,Haibo Zhou,Ruolan Li,Maosen Xu,Chenghua Zhang,Zhengsijia Wu,Youzhou He,Xu Zou,Min Fu,Siping Wei
The technology of photolysis water hydrogen evolution driven by solar energy is considered promising to alleviate the energy crisis. However, the low separation and transfer efficiency of photogenerated charge carriers is still one of the challenges leading to the inferior efficiency of photocatalysis. In this paper, alkyl n-hexanoic acid (HA) and aryl benzoic acid (BA) were used as modulators to synthesize HA-PCN-224 and BA-PCN-224 by solvothermal method, in which TCPP linker was coordinated with Zr-O clusters in accompany with part of reserved modulators, respectively. The photocatalytic hydrogen evolution activity of HA-PCN-224 (225.29 μmol·g −1 ·h −1 ) was 2.86 times as that of BA-PCN-224 (78.78 μmol·g −1 ·h −1 ), which should be ascribed to the higher conductivity confirmed by the electrochemical impedance spectroscopy (EIS). Subsequently, the sample HA-PCN-224(Pd) was synthesized by introducing Pd Ⅱ into the porphyrin center. The Photoluminescence (PL), photocurrent response (i-t), Mott-Schottky characterization, and density functional theory (DFT) calculations show that HA-PCN-224(Pd) has excellent electrical conductivity and unique Pd Ⅱ electron bridge, which can significantly accelerate the carrier separation and transfer efficiency. Surprisingly, with the help of Pd Ⅱ as an electron bridge, the photocatalytic hydrogen production activity of HA-PCN-224(Pd) is as high as 20411.32 μmol·g −1 ·h −1 , which is about 90.60 times as that of the original HA-PCN-224. This work provides us with a novel avenue to modulate the conductivity of MOFs via adjusting monocarboxylic acid regulators and introduce unique Pd Ⅱ as the effective electron bridge to promote excellent separation and transfer efficiency of charge carriers for the application in solar energy-related fields.
研究领域:
材料科学
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光化学
·
催化
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF RARE EARTHS
·
2025
·
IF 7.2
Ming Qian,Jiarui Du,Qichuan Jiang,Wei Tao,Jiayi Zhang,Liang Gong
Early diagnosis and treatment of cancer are key problems for effective cancer therapy. Therefore, the development of multifunctional materials that can integrate imaging and therapeutic functions is of great importance. Lanthanide-doped nanoparticles@metal-organic frameworks (Ln-NPs@MOFs) offer an unprecedented opportunity to address this challenge, by combining the imaging capabilities of rare earth-doped nanoparticles with the photosensitizing properties of MOFs for photodynamic therapy (PDT). Here, we report a near-infrared (NIR) light-responsive nanoparticle based on a zirconium-porphyrin metal-organic framework (PCN-224) for synergistic photothermal and photodynamic therapy. Tetra(4-carboxyphenyl)porphyrin (TCPP) that constitutes the MOF framework serves as a natural photosensitizer, enabling efficient photodynamic therapy through Förster resonance energy transfer (FRET). The dye indocyanine green (ICG) was loaded into the MOF pores to sensitize the Ln nanoparticles, enhancing the upconversion fluorescence by 41 times and the downconversion fluorescence by 5 times, which is beneficial to the biological efficacy for imaging diagnosis. Meanwhile, ICG, a traditional photothermal agent, also imparts photothermal therapy capabilities to the system. Furthermore, the encapsulation of cancer cell membranes not only enhances biocompatibility but also improves the material's targeting towards cancer cells, thereby greatly enhancing the therapeutic effect. This dual-functional material provides a promising platform for the integration of cancer diagnosis and therapy.
研究领域:
生物医学工程
·
纳米医学
·
癌症诊疗一体化
·
光学成像
·
光疗
✓ 已验证
DOI
高影响因子
ACS Nano
·
2022
·
IF 18.027
Ke Li,Chuanchuan Lin,Menghuan Li,Kun Xu,Ye He,Yulan Mao,Lu Lu,Wenbo Geng,Xuemin Li,Zhong Luo,Kaiyong Cai
Ferroptosis is a recently discovered route of regulated cell death that offers the opportunities for the treatment of chemotherapy-resistant tumor indications, but its efficacy can be affected by the glutathione peroxidase 4 (GPX4) and ferroptosis suppressor protein 1 (FSP1) antioxidant mechanisms, posing significant challenges for its clinical translation. In this study, we report a Cu-tetra(4-carboxyphenyl)porphyrin chloride(Fe(III)) (Cu-TCPP(Fe)) metal organic framework (MOF)-based nanosystem for the efficient incorporation of Au nanoparticles (NPs) and RSL3, which can demonstrate enzyme-like activities to universally suppress the antiferroptotic pathways in tumor cells for amplifying ferroptotic damage. Herein, Cu-TCPP(Fe) MOF nanosheets were integrated with Au NPs via in situ nucleation and loaded with RSL3 via π–π stacking, which were eventually modified with polyethylene glycol (PEG) and iRGD for tumor-targeted drug delivery. Specifically, the Au NPs can demonstrate glucose oxidase-like activities for efficient glucose depletion, thus disrupting the pentose phosphate pathway to impede reduced glutathione (GSH) biosynthesis and prevent the recycling of coenzyme Q10 (CoQ10) to CoQ10H2, while Cu species can oxidize GSH into oxidized glutathione (GSSG). These nanocatalytic activities can lead to the simultaneous inhibition of the GPX4/GSH and FSP1/CoQ10H2 pathways and cooperate with the GPX4-deactivating function of RSL3 to cause pronounced ferroptotic damage, thereby providing a strong rationale for the application of ferroptosis therapy in the clinic.
研究领域:
癌症生物学
·
纳米技术
·
治疗学
·
细胞死亡机制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2022
·Vol. 32
·期 17
·2108883
·
IF 19.924
Xiu Zhao,Kaixiang Zhang,Yiyang Wang,Wenxiao Jiang,Hui Cheng,Qiongwei Wang,Tingting Xiang,Zhenzhong Zhang,Junjie Liu,Jinjin Shi
Efficient nuclear DNA damage and release is highly recommended to improve the photodynamic immunotherapy by eliciting innate immune response yet remains challenging. Herein, an intracellular self-assembly driven nucleus-targeted photo-immune stimulator (PIS) with chromatin decompaction function is reported for innate and adaptive antitumor immunity co-activation. The PIS consists of vorinostat (SAHA)-loaded manganese-porphyrin metal-organic framework (Mn (III)-TCPP MOF) with further modification of AS1411 aptamer. The PIS can be efficiently internalized by tumor cells and disassembled under the intracellular overexpressed glutathione (GSH). Notably, the released AS1411 is able to be self-assembled with photosensitizer TCPP in situ within cells, driving nucleus-targeted delivery of TCPP; meanwhile, the loaded SAHA can induce chromatin decompaction, cooperatively promoting TCPP-mediated photodynamic nuclear DNA damage and cytosolic release under laser irradiation. In addition, the released manganese ions (Mn 2+ ) further enhance the cytosolic DNA/cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) pathway mediated innate immunity, which synergizes with PDT-induced immunogenic cell death to achieve co-activation of innate and adaptive immunity. Compared to traditional PDT, the self-assembly driven nucleus-targeted PDT system with chromatin decompaction show significantly enhanced efficacy for inhibiting primary tumor growth and distant metastasis in several xenograft tumor models, mechanistically by promoting the maturation of dendritic cells and tumor infiltration of natural killer cell, cytotoxic T lymphocytes.
研究领域:
癌症研究
·
免疫学
·
药学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）