查找使用阿拉丁产品的科研文献

tert-Butyl-p-benzoquinone induces autophagy by inhibiting the Akt/mTOR signaling pathway in RAW 264.7 cells

Xianghe Meng,Chaosheng Xia,Qin Ye,Xiaohua Nie

tert-Butyl-p-benzoquinone (TBBQ), a metabolite of tert-butylhydroquinone from food, has cytotoxicity, the underlying mechanism of which is not clear. In this study, the viability of RAW 264.7 cells exposed to TBBQ at concentrations of 0.5–10 μg mL−1 was assayed by MTT. Results suggest that TBBQ decreased the viability in a dose-dependent manner. Monodansylcadaverine (MDC) staining results indicate the occurrence of autophagy induced by TBBQ, which was manifested by activation of LC3-II concurrent with the increased levels of Beclin1 and reduced levels of p62. Elevated lipid peroxide and decreased SOD activity by TBBQ exposure suggest the overproduction of ROS, which may account for the increase in the genotoxic stress protein p53. Both upregulation of p53 and reduction of Akt levels inhibited mTOR, which activated autophagy. Addition of 3-MA counteracted the impact of TBBQ on ATG proteins and cell viability. All of these results suggest that TBBQ induces autophagy of RAW 264.7 cells principally by inhibition of the Akt/mTOR signaling pathway, and they implicate ROS in this regulation.

Efficient removal of pentachlorophenol from aqueous solution by 4-tert-butylcalix[8]arene modified thermally sensitive hydrogels

Ying Guo,Bing Liao,Kun Wang,Yangyang Zhao,Qiwen Yong,Hongwei Zhao,Hao Pang

We prepared poly(N-isopropylacrylamide-co-4-tert-butylcalix[8]arene) (PNIPAM-TBCX) hydrogels by copolymerization of N-isopropylacrylamide (NIPAM) with 4-tert-butylcalix[8]arene (TBCX) to capture hazardous pentachlorophenol (PCP) from aqueous solution. Adsorption experiments showed that the adsorption capacities of PNIPAM-TBCX hydrogels reached 1.96, 2.08 and 2.02 mg PCP per 1 g of hydrogel, while the molar percentage ratio of TBCX in the hydrogels was as low as 0.5%, 0.7% and 1%. The equilibrium adsorption of PCP on the hydrogels was studied using different adsorption models. In addition, the PNIPAM-TBCX hydrogel still retained its performance when regenerated several times by immersing in water at 323 K.

Selective production of 2-(tert-butyl)-3-methylphenol from depolymerization of enzymatic hydrolysis lignin with MoS2 catalyst

Yiming Ma,Yushuai Sang,Kai Wu,Qingfeng Liu,Hong Chen,Yongdan Li

Low selectivity and complex product distribution are the main challenges for the utilization of lignin. Herein, the selective production of 2-(tert-butyl)-3-methylphenol (TBC), an antioxidant in the polymer industry, from depolymerization of enzymatic hydrolysis lignin (EHL) on a hydrothermally synthesized MoS 2 catalyst is studied. The total aromatic monomer yield is 124.1 mg/g EHL and the selectivity of TBC is up to 40.3 wt% in methanol at 280 °C under 2 MPa H 2 for 6 h. The FT-IR analysis of products reveals that MoS 2 has a high activity for demethylation, dehydroxylation and alkylation, and the dimer conversions reveal that C-O and C-C bonds in EHL are broken with MoS 2 . The guaiacol and its derivants are identified as the intermediate for formation of TBC in EHL depolymerization according to the effect of time on product distribution and monomer conversion.

N-Doped TiO2–Carbon Composites Derived from NH2-MIL-125(Ti) for Electrochemical Determination of tert-Butylhydroquinone

Tang Jing,Li Jie,Liu Tianna,Tang Wenjing,Li Nali,Zheng Shengbiao,Guo Jiahao,Song Changchun

Electrochemical determination of tert-butylhydroquinone (TBHQ) is vital to food safety due to negative health effects; however, bare electrode of traditional electrochemical sensors generally has a narrow linear range and low sensitivity, limiting their practical application. Accordingly, the nano-architecture fabricated with N-doped TiO 2 –carbon nano-composites (TiO 2 /NC) is prepared by the thermolysis of NH 2 functionalized MIL-125(Ti) metal–organic frameworks (NH 2 -MIL-125(Ti)). TiO 2 /NC composites are firstly developed as the electrochemical material for electrochemical determination of TBHQ. The TiO 2 /NC composites with a highly porous structure, excellent conductivity and electron transportation, and large surface area show remarkable electrochemical oxidation ability for TBHQ. Under optimal conditions, TiO 2 /NC composite-modified electrode presents a broader linear response to TBHQ concentration of 0.05–100 μM with the detection limit as low as 4 nM (S/N = 3). Finally, the sensor platform is implemented directly to determine TBHQ in edible oil for evaluation of its practical application. TiO 2 /NC composites sensor fabricated perform larger linear range with high sensitivity and anti-interference properties, which can be used as a potential for electrochemical determination of TBHQ.

Tert-butylhydroquinone attenuates LPS-induced pyroptosis of IPEC-J2 cells via downregulating HMGB1/TLR4/NF-κB axis

Wenyue Qiu,Xinting Zhang,Xiaoyue Pang,Jianjia Huang,Shuilian Zhou,Ruixia Wu,Rongmei Wang,Zhaoxin Tang,Rongsheng Su

Inflammatory response induced by biological stress usually occurs in weaning piglets, it reduces the production performance of piglets and even causes death. Tert-butylhydroquinone (TBHQ) is a food additive that has the effect of anti-inflammation and anti-oxidation. However, there are few reports related to the protective mechanisms of TBHQ on lipopolysaccharide (LPS) induced injury in intestinal porcine epithelial (IPEC-J2) cells. Quantitative real-time polymerase chain reaction and western blot analysis, respectively, detected the mRNA levels and protein expressions related to pyroptosis, tight junction (TJ) protein and high-mobility group box 1/toll-like receptor 4/nuclear factor kappa-B (HMGB1/TLR4/NF-κB) axis. Localisation and expression of NOD-like receptor pyrin domain containing 3 (NLRP3), HMGB1 and P-NF-κB proteins detected by immunofluorescence. The results showed that TBHQ (12.5 and 25 μM) can increase cell activity and reduce intracellular lactate dehydrogenase (LDH) levels in a dose-dependent manner. LPS significantly decreases cell viability and increases the LDH level. However, pretreatment with TBHQ evidently increases cell viability and decreases the LDH level of IPEC-J2 cells. In addition, treatment with LPS decreased the mRNA level and protein expression of zonula occludens-1, occludin and claudin-1, and increased the mRNA level and protein expression of pyroptosis and HMGB1/TLR4/NF-κB axis. Interestingly, pretreatment with TBHQ increased the TJ protein expressions as well as decreased the mRNA level and protein expressions of pyroptosis and HMGB1/TLR4/NF-κB axis. Moreover, the results of immunofluorescence showed that TBHQ significantly reduced the expression of NLRP3, HMGB1 and P-NF-κB in LPS-induced injury of IPEC-J2 cells. Therefore, we come to the conclusion that TBHQ attenuates LPS-induced pyroptosis in IPEC-J2 cells through downregulation of the HMGB1/TLR4/NF-κB axis, TBHQ may become a potential feed additive for preventing inflammatory diarrhoea in piglets.

Synthesis and comparative study of thermoplastic polyetherimides derived from hydroquinone diphthalic anhydride isomers with tert-butyl group

Jie Li,Shufeng Liu,Ge Zhu,Chunshan Lu,Yuxia Ba,Guofei Chen,Xingzhong Fang

Two isomeric dianhydride monomers, 1,4-bis(3,4-dicarboxyphenoxy)-2- tert -butylbenzene dianhydride (4,4′-TBHQDA) and 1,4-bis(2,3-dicarboxyphenoxy)-2- tert -butylbenzene dianhydride (3,3′-TBHQDA), were successfully synthesized from tert -butylhydroquinone and nitrophthalonitrile. Two series of polyetherimides (PEIs) were synthesized by chemical imidization using the above dianhydrides with four common commercially available diamines. The effects of dianhydrides with isomerization and tert -butyl group on the thermal properties, optical properties, melt processability, solubility, and mechanical properties of PEIs were systematically studied. Among them, PEI-2(a-d) derived from 3,3′-TBHQDA displayed better comprehensive performance than other PEIs, which showed well T g s at 234–261 ℃, good optical properties of 82–86 % at 450 nm, extremely low melt viscosities of 30–59 Pa·s at 400 ℃, excellent solubility and good mechanical properties (tensile modulus of 2.7–3.5 GPa, tensile strengths of 90–97 MPa and elongations at break of 3.4–4.8 %).

Hydro-Solvothermal Synthesis of NaX Zeolite Membranes for the Separation of MeOH/Methyl tert-Butyl Ether Mixtures

Xue-Ling Wei,Bo Song,Yu-Ling Luo,Jun-Fang An,Jun-Bin Zhang,Meng Pan,Xingyang Li

Zeolite membranes are emerging as attractive candidates for the separation of methanol/methyl tert-butyl ether (MTBE) mixtures, while the random deposition of silicon aluminum components on the surface of the support can lead to the poor quality of zeolite membranes, restricting the use of zeolite membranes in separation and purification applications. In this study, the deposition process of the silicon aluminum components was controlled, and compact NaX zeolite membranes with great pervaporation performance for methanol/MTBE mixtures were synthesized using water–organic solvent synthesis solutions. Systematic research showed the existence of a stable “liquid film” on the surface of the support controlling the formation environment of zeolite membranes during their synthesis; organic solvents that partly replaced water in the synthesis solution improved the quality of the zeolite membranes. The water–organic solvent system could not only decrease the nucleation and crystallization rate of zeolite particles in the main body of the synthesis solution and reduce the consumption of silicon aluminum components in the main body of the synthesis solution but also promote the migration of silicon aluminum components to the “liquid film” on the surface of the support from the main body of the synthesis solution, thus promoting the growth of zeolite membranes. The zeolite membrane synthesized in the 20(v) % 1,3-propanediol synthesis solution exhibited a MeOH/MTBE separation factor greater than 10,000 with a permeation flux of 1.6 kg·m–2·h–1, indicating that a compact zeolite membrane was grown on the support. In this work, the formation of zeolite membranes was controlled by adding an organic solvent to the synthesis solution to adjust the deposition process of silicon aluminum components to the support and a new method for the preparation of compact membranes was developed.

Effects of 4-tert-butylpyrocatechol and tea polyphenol on growth, physiology and antioxidant responses in Microcystis aeruginosa

Yitong Lu,Xinxin Jiang,Hongzhou Xu,Chengrong Liu,Yanzhen Song,Kuiquan Pan,Lixin Wang,Liang Du,Haixia Liu

Global warming has increased the frequency of Microcystis aeruginosa blooms, leading to the deterioration of water quality and loss of biodiversity. Therefore, developing effective strategies for controlling M. aeruginosa blooms has become an important research topic. Plant extracts, 4‑tert-butylpyrocatechol (TBC) and tea polyphenol (TP) are commonly used for water purification and to increase fish immunity, which have great potential to inhibit cyanobacterial blooms. The inhibitory effects of TBC and TP on M. aeruginosa were investigated in terms of growth characteristics, cell membrane morphology, physiological, photosynthetic activities, and antioxidant enzymes activities . The results showed that TBC and TP inhibited the growth of M. aeruginosa by decreasing the chlorophyll fluorescence transients or increasing the antioxidant enzymes activities of M. aeruginosa . TBC damaged the cell morphology of M. aeruginosa , reduced extracellular polysaccharides and protein contents, and up-regulated the antioxidant activity-related gene ( sod and gsh ) expressions of M. aeruginosa . TP significantly decreased the photosynthetic pigment content, influenced the phycobiliprotein content, and strongly down-regulated the photosynthesis-related gene ( psbA, psaB, and rbcL ) relative expressions of M. aeruginosa . TBC caused significant oxidative stress , dysfunction of physiological metabolic processes, and damaged crucial biomacromolecules (e.g., lipids, proteins and polysaccharides), prompted the loss of cell integrity, ultimately leading to the death of M. aeruginosa . However, TP depressed photosynthetic activities and consequently inhibited the transfer of electrons, affected the electron transfer chain , decreased the photosynthetic efficiency , and eventually caused the death of M. aeruginosa cells. Our study showed the inhibitory effects and algicidal mechanisms of TBC and TP on M. aeruginosa , and provide a theoretical basis for restrain the overgrowth of M. aeruginosa .

Design of polyarylates containing tert-butyl cyclohexyl bulky side group with high transparency and low dielectric properties

Yu Zhang,Guang-ming Yan,Jie Yang,Gang Zhang

A new kind of novel low dielectric constant ( D k ) and high transparency polyarylate had been synthesized through interfacial polycondensation with 1,1′- bis (4-hydroxybenzene)-4-tert-butylcyclohexane (Bt-BPZ) containing tert-butyl cyclohexyl bulky side group. As we expect, that the introduction of tert-butyl cyclohexyl with large free volume is proved to be an efficient strategy for obtaining low dielectric constant and high transparency polyarylate. The optimal dielectric property is obtained with 90% Bt-PZA in polyarylate (Bt-PZA91), which show a D k as low as 2.14 at 1 MHz. Meanwhile, Bt-PZA91 also demonstrates good transmittance of 81.08%–88.34% at 450 nm and 78.67–88.26 at 400 nm. The cut-off wavelength of Bt-PZA91 is 304 nm, which is 16 nm shorter than commercial product U-100 ( λ c : 320 nm). In addition, the resultant polyarylates showed good comprehensive performance such as T g of 202.2–220.5°C, T d 5% (the initial thermal degradation temperature) of 455.4–468.4°C, tensile strength of 64.7–86.8 MPa and low water absoption (0.41% of Bt-PZA91). Also it was found to can be thermal process, that its convenient thermoplastic processability makes it a great candidate for light-electronic industry, 5G communication, and we believe that Bt-PZA will have a broad application prospect.

A multicomponent propane monooxygenase catalyzes the initial degradation of methyl tert-butyl ether in Mycobacterium vaccae JOB5

Yiyang Chen,Hao Ren,Xiangyu Kong,Hao Wu,Zhenmei Lu

Formation of adducts during digestion triggered dietary protein for alleviating cytotoxicity of 2-tert-butyl-1,4-benzoquinone

Ningxiang Yu,Zeyi Wu,Yijue Wang,Abel Wend-Soo Zongo,Xiaohua Nie,Yuanchao Lu,Qin Ye,Xianghe Meng

2- tert -butyl-1,4-benzoquinone (TBBQ), a degradation product of lipid antioxidant Tert-Butylhydroquinone (TBHQ), is a new hazardous compound in foods . This study investigated whether co-ingestion of dietary protein and TBBQ can alleviate the toxicity of TBBQ. The results indicated that soy protein isolate, whey protein isolate, and rice protein significantly reduced the residual amount of TBBQ during simulated gastrointestinal digestion. This result was attributed to the excellent elimination capacity of the released amino acids for TBBQ through formation of adducts. Among 20 amino acids, histidine , lysine, glycine, and cysteine showed better elimination capacity for TBBQ; they can eliminate 92.1%, 89.4%, 86.1%, and almost 100%, respectively, in 5 min at pH 8.0. Further study indicated that amino acids with lower ionization constant exhibited greater TBBQ elimination capacity. In addition, incubation of the cells with 50 μM TBBQ for 12 h decreased the cell viability to 28.95 ± 3.25%; while amino acids intervention was involved in the alleviation of TBBQ cytotoxicity via decreasing ROS. Particularly, cysteine showed 100 times more TBBQ detoxifying capacity than other amino acids. This work could provide a theoretical basis for the potential application of amino acids for detoxifying TBBQ in the food industry.

Study on initiating approach of (3-(tert-butylperoxy)propyl) trimethoxysilane on the polymerization of acrylonitrile as an initiator*

Yazhen Wang,Yutao Di,Shaobo Dong,Zijian He,Xueze Zhang,Jia Shi,Tianyu Lan

(3-(tert-butylperoxy)propyl) trimethoxysilane (TBPT), is a tailor-made new style silane coupling agent with peroxide group, which have ability of initiating polymerization. This study used TBPT to generate free radical, and initiated the polymerization of acrylonitrile (AN), thereby forming polyacrylonitrile (PAN) in two approaches, thermal initiation system and redox initiation system. Meanwhile this study bonded TBPT onto nano-TiO2 to get modified nano-TiO2 by means of the coupling function of TBPT, and then made the peroxide group of the modified nano-TiO2 decompose and initiate the polymerization of AN in thermal initiation system and redox initiation system respectively. The products were investigated and analyzed by FTIR, XPS and TG. The result showed that on one hand, in the products of the thermal initiation there was PAN, which both attached and unattached to the modified nano-TiO2; on the other hand, in the products of the redox initiation system the PAN unattached to the modified nano-TiO2 was produced, while the PAN attached to the modified nano-TiO2 was not.