JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Yuqing Qin,Aroosha Faheem,Yonggang Hu
The excess residues of fluoride ions cause serious human health problems, making their detection highly valuable. In this work, a whole-cell-based biosensor was presented for the detection of fluoride ions, which can inhibit the color reaction of 3,3′,5,5′,-tetramethylbenzidine (TMB) catalyzed by the CotA-laccase of spore surface. This reaction for the detection of fluoride ions could be read out through UV–vis spectrophotometer, smartphone, or standard colorimetric card within 10 min. Under optimum conditions, a linear range of 1–600 μmol L –1 with a detection limit of 0.12 μmol L –1 (3σ/k) was achieved for fluoride ions detection by using UV–vis spectrophotometer. The biosensor coupling with smartphone had a good linear response to fluoride ions concentration in the range of 5–600 μmol L –1 with LOD of 0.90 μmol L –1 (3σ/k). The standard colorimetric card can be directly used for recognizing the fluoride ions level via naked-eyes. A portable kit based on a colorimetric card and smartphone was developed and has been successfully applied for fluoride ions monitoring in surface waters and groundwater. This developed method has several advantages such as rapid, outstanding selectivity and anti-interference, low-cost, ease of operation and storage, and eco-friendliness, meeting the demands of point-of-care testing of fluoride ions and disease prevention.
研究领域:
环境科学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Shixian Lin,Yuehan Ouyang,Wensheng Lin,Xingwu Zhou,Mengyao Miao,Erzhuo Cheng,Yong Jiang,Zixiang Meng,Man Jin,Sen Zhang,Shiqing Li,Xiaoyu Lv,Shile Chen,Yuchen Guo,Jiayi Zhang,Kaiyin Cai,Zishan Lin,Fanwen Yang,Jixiang Zhu
Microneedle (MN) patches have shown great potential for interstitial fluid (ISF) sampling and subsequent analytes detection, providing an alternative pathway for clinical diagnosis based on blood tests. However, MNs based detection is generally based on separate downstream assays, which cannot provide the detection in real-time. Specifically in the context of glucose monitoring, it is desirable to know the glucose level rapidly so as to guide the subsequent treatment in patients. Here, we designed a double layer MNs patch with the crosslinked gelatin methacryloyl (GelMA) tips loaded with glucose oxidase (GOx), whereas the substrate loaded with horseradish peroxidase (HRP), and 3,3′,5,5′-tetramethylbenzidine (TMB). The MNs tips allow for the ISF sampling and detect the glucose in situ via an enzymatic cascade reaction. We used 3D printing technology for MNs molding, which facilitate the customizable MNs design and optimization. Furthermore, we improved the detection sensitivity and accuracy by optimizing the microenvironment of the GelMA and the concentration of each enzymes. The performance of this MNs patch is validated in extracting and detecting glucose using an agarose gel based skin model. The results showed that within the glucose concentration range of 1.7–21 mmol/L, the MNs patch could accurately measure the unknown glucose concentration through the color values of red, green, and blue (RGB) obtained from scanned images. The all-in-one MNs patch with colorimetric functionality is easy to use and does not require specialized personnel or large-scale equipment support, making it highly promising for clinical translation and providing real -time blood glucose monitoring for patients.
研究领域:
生物医学工程
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材料科学
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分析化学
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医疗器械
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jiaxi Deng,Yiyao Luo,Jia Zheng,Zirui Yuan,Yi Ma,Huibo Luo,Xiaogang Luo,Danqun Huo,Changjun Hou
Baijiu represents the pinnacle of traditional Chinese culinary culture. The influence of Baijiu is evident in various literary works, rituals and banquets, dishes and healthcare, and numerous other areas. The reduction of substances plays a pivotal role in the flavor, aroma, and quality control of Baijiu. Therefore, the present work focuses on Baijiu and the reducing substances in it. In this study, CDs@ZrFe-MOF and three distinct catalase nanase substrates (ABTS, OPD, and TMB) were employed to create fluorescence and colorimetric dual-mode sensor arrays. The oxidation of the enzyme substrate into the appropriate oxidation substrate is facilitated by CDs@MOF in the presence of H₂O₂. The reducing substance effectively inhibits the formation of the oxidation substrate, resulting in alterations in fluorescence and UV intensity. The dual-mode sensor array was found to be effective in detecting 15 different types of reducing chemicals. Furthermore, the colorimetric and fluorescence sensor array demonstrated exceptional detection capability in detecting 19 different types of Luzhou-flavor liquor samples, achieving a 100% accuracy rate. This study will provide an accurate and rapid method for differentiating Baijiu.
研究领域:
分析化学
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纳米技术
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传感器技术
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensing and Bio-Sensing Research
·
2024
·
IF 5.4
Lei Wang,Nana Jin,Meixuan Li,Jianhan Lin
Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 10 1 to 1.3 × 10 4 CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.
研究领域:
微生物学
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生物技术
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即时检测
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
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IF 9.518
Ying Gan,Ning Hu,Chuanjiang He,Shuqi Zhou,Jiawei Tu,Tao Liang,Yuxiang Pan,Dmitry Kirsanov,Andrey Legin,Hao Wan,Ping Wang
Urolithiasis commonly occurs in kidney and ureteral, and may cause local organ/tissue damage, even kidney failure. The incidence of this disease is increasing worldwide, in which calcium oxalate is the major composition forming the urinary calculus. Therefore, to monitor this disease for the prevention and treatment, measuring the oxalate in the urine is of great significance. Here, a rapid and sensitive colorimetric method was developed based on 3,3',5,5'-tetramethylbenzidine-manganese dioxide (TMB-MnO 2 ) nanosheets for oxalate detection. MnO 2 nanosheets acted as an efficient biomimetic oxidase to catalyze the reaction with TMB and oxalate. Pale yellow TMB can be oxidized to blue oxide TMB catalyzed by BSA-stabilized MnO 2 nanosheets, and oxalate can selectively inhibit this reaction by consuming and reacting with MnO 2 nanosheets, thus achieving the quantitative detection of oxalate. Moreover, a home-made bionic electronic-eye (E-eye) system was developed as a portable in-situ detection platform to efficiently measure the oxalate concentrations in 10 s by direct photographing. By optimizing experimental conditions, this method shows a wide linear range (7.8 μM to 250 μM) and a low detection limit (0.91 μM) for oxalate detection. Besides, this method exhibits high selectivity even with 80-fold interfering chemicals. Furthermore, the performance of the method was validated by testing the artificial urine samples, indicating its great potential for monitoring and diagnosis of urolithiasis in point-of-care applications.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-10
·
IF 6.408
Wang Lijun,Zhou Hong,Wu Xue,Song Yanfei,Huang Yukun,Yang Xiao,Chen Xianggui
A novel colorimetric aptasensor has been developed for highly sensitive tetracycline (TC) detection based on the peroxidase-like activity of Fe 3 O 4 @Cu nanoparticles and “sandwich” oligonucleotide hybridization. The Fe 3 O 4 @Cu nanoparticles with high peroxidase-like activity were successfully synthesized under mild conditions. Then, a “sandwich” oligonucleotide hybridization probe (a short amino-modified complementary sequence of a portion of the TC aptamer (cDNA1), TC aptamers, and a long complementary to 5′ terminal TC aptamer sequence (cDNA2)) was created in 96-wells plates via DNA hybridization in the absence of TC from the detection system. The unique “sandwich” oligonucleotide hybridization probe adsorbed large numbers of Fe 3 O 4 @Cu nanozymes while further enhancing its peroxidase-like activity. Based on the 3,3′,5,5′-tetramethylbenzidine (TMB)-hydrogen peroxide (H 2 O 2 ) reporting system, the blue color of the solution decreased linearly with the increase of TC concentration, ranging from 10 −3 to 10 3 μg/L with an ultralow limit of detection (LOD) of 0.91 ng/L (2 pM). The proposed method was successfully applied to detect TC in spiked milk samples, with recoveries of 81.8 to 112%, demonstrating the excellent potential for highly sensitive TC detection in milk. Graphical abstract
研究领域:
分析化学
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纳米技术
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL SCIENCES
·
2020
·Vol. 132
·期 1
·1-10
·
IF 1.406
Zhao Lingzhi
Abstract The development of efficient methods for alkaline phosphatase (ALP) activity assay is of great importance in clinical diagnosis and many biomedical applications. In this work, the authors create two strategies for colorimetric detection of ALP activity. The first strategy is developed by using directly manganese dioxide (MnO 2 ) nanosheets as colorimetric probes. The second strategy is developed by using MnO 2 and the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) as colorimetric probes based on the catalytic reduction of oxidized TMB. The first strategy has a linear response in 10 U L −1 to 80 U L −1 with 3.0 U L −1 detection limit while the second one has a linear response in 7 U L −1 to 100 U L −1 with 0.05 U L −1 detection limit. The method based on MnO 2 -TMB which have lower detection limit and higher sensitivity was applied to monitor ALP activity in whole Hela cells lysate. In addition, based on redox properties, MnO 2 -TMB sensing system was successfully used in detecting reducing substances such as ascorbic acid, Na 2 S and so forth. Conceivably, MnO 2 -TMB sensing system is not limited to ALP activity assay but has a wide scope to be applied in assays of the total antioxidant capacity of dietary substances and biological fluids. Graphic Abstract Two strategies were created for colorimetric detection of ALP activity: The first strategy is developed by using directly manganese dioxide (MnO 2 ) nanosheets as colorimetric probes, and the second strategy by using MnO 2 and the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) as colorimetric probes based on the catalytic reduction of oxidized TMB.
研究领域:
分析化学
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生物化学
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生物医学工程
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临床诊断
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Hualin Yang,Qi Zhang,Xiaolei Liu,Yuying Yang,Yong Yang,Mingyuan Liu,Peiwu Li,Yu Zhou
Fumonisins (FBs) exist widely in crops, foods and feeds. Consumption of FBs contaminated corn can cause oesophageal cancer. So it is necessary to develop sensitivity methods for its detection. Here, we report an enhanced assay for rapid and ultra-sensitive detection of FBs based on nanomagnetic beads (NMBs) and antibody-biotin-streptavidin-HRP. Because antibody-BNHS can bind with several number of streptavidin-HRP, the signal amplification of the catalytical oxidation of TMB was enhanced. The detection limit of sensor was down to 0.21 ng mL −1 with a linear range from 0.31 to 162.42 ng mL −1 . Since NMBs provide a nearly “in solution” reaction, they lead to a shortened reaction time (22 min) than that of flat solid-phase based traditional assay. Furthermore, the recoveries obtained by standard FBs spiked to maize samples were from 100.6 to 107.3%. This enhanced assay supplied a rapid, sensitive and reliable method for detection of FBs in maize.
研究领域:
食品安全
·
分析化学
·
生物技术
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 48
·485702
·
IF 3.874
Rui Cai,Xinshuang Gao,Chenqi Zhang,Zhijian Hu,Yinglu Ji,Jianbo Liu,Xiaochun Wu
Surface chemistry control is a key means to improve substrate selectivity and enhance catalytic activity of nanozymes, a kind of novel artificial enzymes. Herein, we demonstrated that apart from chemical properties of functional groups, their spatial distance to the catalytic sites is also very important to improve the catalytic performance of nanozymes. Using cetyltrimethylammonium bromide (CTAB) coated gold nanorods (AuNR) as the example, we showed that cysteine (Cys) surface modification can greatly enhance the peroxidase activity of AuNR for the oxidation of substrate 3,3',5,5'-tetramethylbenzidine (TMB). By using cysteine derivatives, the key role of the carboxylic group in cysteine is revealed in improving substrate binding and activity enhancement. The electrostatic interactions of carboxylic groups from adsorbed cysteine molecules with protonated amino groups of TMB bring TMB molecules to the surface Au active sites and thus markedly increase catalytic activity. In contrast, despite having two carboxylic groups, glutathione (GSH) surface modification only leads to quite limited improvement of catalytic activity. We speculated that due to large molecular size of GSH, the spatial distance between TMB-GSH and Au is larger than that between TMB-Cys and Au. Furthermore, Raman characterization indicated that at high Cys coverage, they form patches on rod surface via zwitterionic interactions, which may give additional benefits by decreasing the steric hindrance of TMB diffusion to surface Au atom sites. In all, our study highlights the importance of fine surface tuning in the design of nanozymes.
研究领域:
纳米技术
·
催化
·
生物化学
·
表面化学
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shuangshuang Wei,Chengyang Zhang,Shujuan Zhang
Peroxidase-mimicking nanozymes have revolutionized biomedicine and environmental catalysis, but their pH-dependent catalytic behavior remains a blind spot in activity evaluation and application design. Here, a framework is presented for understanding and harnessing pH effects through a synergistic interplay of interfacial electrostatics and proton-coupled electron transfer (PCET) process. Contrary to the entrenched “pH 4.0 gold standard,” the proposed multi-index evaluation matrix for activity evaluation reveals that platinum nanozymes achieve peak activity at pH <2.5 via proton-triggered surface reconstruction and optimized PCET energetics. This insight enables a pH-adaptive bio- and contaminant-sensing strategy: by leveraging the signals of the double-electron oxidation product of 3,3′,5,5′-tetramethylbenzidine (TMB 2 ⁺) at pH 2.5, a 47-fold increase in glutathione determination sensitivity is achieved over the traditional single-electron oxidation product (TMB⁺ • ) analysis at pH 4.0. This study not only recalibrates nanozyme evaluation benchmarks but also pioneers proton-engineered catalysis for environmental applications.
研究领域:
生物催化
·
纳米化学
·
环境传感
·
生物分析
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jintao Yi,Zhenhua Wu,Nani Fu,Xianqin Han,Ting Yan,Jiaying Zheng
Amoxicillin (AMO), a broad penicillin antibiotic, has been widely employed for the treatment of bacterial infections. However, the unreasonable use of amoxicillin may cause a serious threat to human health and its visual determination remains a challenge. Herein, a MnO2@CQDs probe was used for the dual-mode detection of AMO, which was synthetized by CQDs doped in MnO2 nanosphere. In the colorimetric mode, MnO2@CQDs oxidized colorless 3, 3ʹ, 5, 5ʹ-tetramethylbenzidine (TMB) into yellow oxidized TMB (oxTMB). In the presence of AMO, the AMO could deoxidize oxTMB to cause multiple color changes from yellow to colorless. In the fluorescence mode, MnO2@CQDs initially exhibited quenched fluorescence due to the presence of MnO2. Upon the addition of AMO, MnO2 was reduced to Mn2⁺ ions, then CQDs were released and recovered fluorescence at the emission wavelength of 450 nm. The colorimetry detection range was wide from 0.005 to 0.09 μM, and the detection limit was 1.6 nM. With increasing concentration of AMO, the color changed from yellow to colourless which allowed naked eye observation. The fluorescence method was rapid, requiring only 1 min, and exhibited a linear detection range from 0.003 to 0.045 μM, with a detection limit as low as 1 nM. In addition, a paper-based sensing platform was constructed, where fluorescence intensity transitioned from dark to blue with increasing AMO levels. The RGB analysis demonstrated that AMO concentration could be discriminated effectively using a smartphone-assisted detection system. The MnO2@CQDs sensor showed excellent selectivity and specificity for AMO colorimetric and fluorescence detection. Moreover, the probe exhibited good practicability in tap water, milk and serum, with results comparable to those obtained by high-performance liquid chromatography. Overall, the MnO2@CQDs dual-mode probe provides an efficient, convenient and rapid strategy for the visual and quantitative detection of AMO in complex environments.
研究领域:
分析化学
·
环境监测
·
食品安全
·
生物传感
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-9
·
IF 5.705
Wang Yanying,Yang Li,Liu Yaqin,Zhao Qingbiao,Ding Fang,Zou Ping,Rao Hanbing,Wang Xianxiang
A composite consisting of NiCo 2 S 4 and reduced graphene oxide (rGO) was prepared via a hydrothermal process. Compared to individual NiCo 2 S 4 nanomaterials or reduced graphene oxide, the composite exhibits enhanced oxidase-like activity. It is found that dopamine (DA) inhibits the ability of NiCo 2 S 4 -rGO to oxidize the substrate 3,3′,5′,5′-tetramethylbenzidine (TMB) to form blue colored ox-TMB. Based on these findings, a colorimetric method for determination of DA was worked out. The absorption, best measured at 652 nm, increases linearly in the 0.5–100 μM DA concentration range, and the limit of detection is 0.42 μM. This method was successfully applied to the detection of DA in spiked human serum samples.
A hierarchical NiCo 2 S 4 -rGO composite was prepared through two-step hydrothermal process. It exhibits enhanced oxidase-like activity which, however, is inhibited by dopamine (DA). Hence, less blue colored ox-TMB is formed by oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of dopamine.
研究领域:
材料科学
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）