FOOD CHEMISTRY
·
2023
·
IF 8.8
Haohong Lai,Pingtao Ming,Maoqiang Wu,Shumei Wang,Duanping Sun,Haiyun Zhai
In this research, a signal-off electrochemical aptasensor with high sensitivity was constructed for trace detection of zearalenone (ZEN). Specifically, Ce-based metal–organic framework and multi-walled carbon nanotubes nanocomposite was functionalized with polyethyleneimine ( [email protected] ) and served as sensing platform for its high surface area and excellent electrochemical active. Subsequently, toluidine blue (TB) was electrodeposited as the signal probe, and [email protected] nanoparticles ( [email protected] ) were dropped for the attachment of aptamer (ZEA). In the presence of ZEN, the ZEA would specifically recognize and combine with the target, causing a decrease of electrochemical signal from TB. Under the optimal conditions, the aptasensor exhibited good linear relationship for ZEN in a concentration range from 5.0 × 10 −5 to 50.0 ng/mL, while the limit of detection (LOD, S/N = 3) and limit of quantitation (LOQ, S/N = 10) were 1.0 × 10 −5 ng/mL and 2.9 × 10 −5 ng/mL, respectively. Ultimately, the aptasensor was successfully applied into ZEN detection in semen coicis real samples.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-13
·
IF 5.833
Huang Hao,Camarada María Belén,Wang Dan,Liao Xiaoning,Xiong Wanming,Du Juan,Xiong Jianhua,Hong Yanping
A novel electrochemical sensor has been fabricated for sensitive determination of zearalenone (ZEA) in food samples based on molybdenum disulfide quantum dots (MoS 2 QDs) and two-dimensional titanium carbide (2D-Ti 3 C 2 T x MXene) co-modified multi-walled carbon nanotube (MWCNT) heterostructure. Physical and electrochemical characterizations reveal that 2D-Ti 3 C 2 T x and MoS 2 QDs co-modified MWCNTs yields synergistic signal amplification effect, together with large specific surface area and excellent conductivity for the heterostructure, endowing the developed sensor with high detection performance to ZEA. Under optimized conditions, the sensor shows a wide linear range from 3.00 to 300 ng mL −1 and a low limit of detection (LOD) of 0.32 ng mL −1 , which is far lower than the maximum residue limits (MRLs) settled by the European Commission. In addition, it exhibits excellent selectivity, high reproducibility with a relative standard deviation (RSD) of 1.1%, and good repeatability (RSD 1.1%). In real sample analysis recoveries ranged from 94.8 to 105% showing the proposed electrochemical sensor has high potential in practical applications. This work presents an effective and valuable pathway for the use of novel heterostructure in the biosensing field. Graphical abstract
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FOOD CONTROL
·
2020
·
IF 4.258
Silu Hou,Jingjiao Ma,Yuqiang Cheng,Hengan Wang,Jianhe Sun,Yaxian Yan
The mycotoxins in grains is a serious threat to the health of humans and animals. In this study, we demonstrated an approach for development of a fast and sensitive fluorescent immunochromatographic assay for fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and zearalenone (ZEN) detection using quantum dots nanobeads (QDNBs, 100 nm). Amplification of the fluorescence signal of the quantum dot by nanosphere coating can significantly improve the sensitivity of immunochromatographic test strip. The detection probes (FB1-McAb@QDNBs, DON-McAb@QDNBs, and ZEN-McAb@QDNBs) were prepared using the QDNBs with 615 nm emissions coupling with three monoclonal antibodies (McAb) against FB 1 , DON, and ZEN, respectively, in a competitive immunochromatography platform. The surface of the QDNBs is modified with a carboxyl functional group, which facilitates the coupling of the antibody and significantly improves the sensitivity of test strip. Compared with traditional ELISA , the immunoassay based on the McAb@QDNBs is more sensitive, with IC 50 of 12.66 ng/mL (FB 1 ), 2.97 ng/mL (DON), and 0.87 ng/mL (ZEN), of which the linear range were from 2.295 ng/mL to 69.867 ng/mL (FB 1 ), 0.465 ng/mL to 16.19 ng/mL (DON), and 0.295 ng/mL to 2.575 ng/mL (ZEN), respectively. The accuracy, repeatability, and specificity of the established test strip was evaluated by testing the spiked and natural samples. Furthermore, a high agreement was observed between the developed test strips and LC-MS/MS in simultaneously and quantitative detection of three mycotoxins. In brief, the developed approach was feasible and could be employed for simultaneously and quantitative mycotoxins detection in agricultural products.
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TALANTA
·
2019
·
IF 4.916
Yan Zhang,Donghao Liu,Jun Peng,Yanru Cui,Yi Shi,Hua He
A novel magnetic surface molecular imprinted polymers with 2, 4, 6-trisacrylamido-3, 5-triazine (TAT) as a functional monomer was successfully synthesized and used for the enrichment and determination of zearalenone . The molecular imprinting is reported herein at first time for application of zearalenone in wheat. The magnetic imprinted materials possessed excellent magnetism and uniform appearance, which were characterized by fourier transform infared spectroscopy and transmission electron microscope. The results proved the magnetic molecular imprinted polymers was successfully prepared. The magnetic molecular imprinted polymers exhibited satisfactory sensitivity, stability and potential reusability. The binding affinity was investigated by selectivity experiment, which possessed high selectivity. To obtain the optimal application conditions, the amount of adsorption, extraction time, elution solvent and time were optimized. The limited detection of zearalenone was 0.55 ng g −1 and the recoveries of zearalenone were 92.1–96.0%. The relative standard deviation was lower than 5.4%. This indicated that a simple, efficient and low-cost method was established and successfully applied in spiked wheat sample.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Imran Mahmood Khan,Sobia Niazi,Ye Yu,Ali Mohsin,Bilal Sajid Mushtaq,Muhammad Waheed Iqbal,Abdur Rehman,Wasim Akhtar,Zhouping Wang
Mycotoxins posit serious threats to human and animal health, and numerous efforts have been performed to detect the multiple toxins by a single diagnostic approach. To best of our knowledge, for the first time, we synthesized an aptamer induced “turn on” fluorescence resonance energy transfer (FRET) biosensor using dual-color gold nanoclusters (AuNCs), l-proline, and BSA synthesized AuNCs (Lp-AuNCs and BSA-AuNCs), with WS2 nanosheet for simultaneous recognition of aflatoxinB1 (AFB1) and zearalenone (ZEN) by single excitation. Here, AFB1 aptamer stabilized blue-emitting AuNCs (AFB1-apt-Lp-AuNCs) (at 442 nm) and ZEN aptamer functionalized with red-colored AuNCs (ZEN-apt-BSA-AuNCs) (at 650 nm) were employed as an energy donor and WS2 nanosheet as a fluorescence quencher. With the addition of AFB1 and ZEN, the change in fluorescence intensity (F.I) was recorded at 442 and 650 nm and can be used for simultaneous recognition with a detection limit of 0.34 pg mL–1 (R2 = 0.9931) and 0.53 pg mL–1 (R2 = 0.9934), respectively. Most importantly, the semiquantitative determination of AFB1 and ZEN can also be realized through photovisualization. The current approach paves a new way to develop sensitive, selective, and convenient metal nanocluster-based fluorescent “switch-on” probes with potential applications in multipurpose biosensing.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 18
·7044-7050
·
IF 3.069
Qiuzheng Du,Pu Wu,Fan Hu,Geyuan Li,Jianrong Shi,Hua He
The work describes an opto-sensor for the highly sensitive detection of the zearalenone (ZON) in wheat. A molecularly imprinted polymer layer was coated onto the surface of a luminescent metal–organic framework with cyclodo-decanyl-2,4-dihydroxybenzoate (CDHB), an analog of ZON, chosen as the template and 3-aminopropyltriethoxysilane (APTES) as the functional monomer. The obtained MOF@MIP (MMIP) was fully characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), N2 adsorption–desorption measurements, and Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, the relative fluorescence intensity of MMIP decreased linearly with a concentration of ZON ranging from 0.05–1.0 mg L−1, with a detection limit of 0.018 mg L−1 and a good recovery of 95.53–98.01% (RSD < 4.7%). The sensor was stable and selective. Ultimately, the rapid and accurate detection of zearalenone in wheat was successfully realized.
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Materials Today Communications
·
2025
·
IF 4.5
Zhilei Zhao,Yu Li,Wenlei Zhai,Liuqing Wang,Qianqian Li,Yudan Wang,Meng Wang
The contamination of highly toxic zearalenone (ZEN) in food and the environment poses a serious threat to human safety. However, it is still challenging to establish an efficient, inexpensive and stable ZEN degradation technology. In this study, a Fe single-atom nitrogen-doped porous carbon (Fe-N-PC) was synthesized by high-temperature pyrolysis and used for efficient adsorption and degradation of ZEN. Fe-N-PC showed a high adsorption capacity (q m = 78.2 mg/g) for ZEN, and the Fe-N-PC/peroxymonosulfate (PMS) system could remove 99.39 % of ZEN, in which 1 O 2 played a major role in ZEN degradation. Furthermore, four degradation products of ZEN were identified and possible degradation pathways were hypothesized. For practical applications, Fe-N-PC was applied to remove ZEN from Coix seed extracts, and the results showed that Fe-N-PC was able to detoxify ZEN from Coix seed (92.32 %). Additionally, Fe-N-PC and ZEN degradation products showed low biological toxicity. In a word, the Fe-N-PC/PMS system was used for efficient adsorption and degradation of ZEN, and has been practically applied in complex food matrices, demonstrating Fe-N-PC may be a promising material for the removal of ZEN in the food field.
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Biosensors-Basel
·
2024
·Vol. 14
·期 7
·355
·
IF 4.9
Jingyang Zhang,Banglei Zhu,Xiaoyu Zhang,Yuan Peng,Shuang Li,Dianpeng Han,Shuyue Ren,Kang Qin,Yu Wang,Huanying Zhou,Zhixian Gao
Mycotoxins are secondary products produced primarily by fungi and are pathogens of animals and cereals, not only affecting agriculture and the food industry but also causing great economic losses. The development of rapid and sensitive methods for the detection of mycotoxins in food is of great significance for livelihood issues. This study employed an amino-functionalized zirconium luminescent metal–organic framework (LOF) (i.e., UiO-66-NH2). Click chemistry was utilized to assemble UiO-66-NH2in a controlled manner, generating LOF assemblies to serve as probes for fluorescence-linked immunoassays. The proposed fluoroimmunoassay method for Zearalenone (ZEN) and Fumonisin B1 (FB1) detection based on the UiO-66-NH2assembled probe (CLICK-FLISA) afforded a linear response range of 1–20 μmol/L for ZEN, 20 μmol/L for FB1, and a very low detection limit (0.048–0.065 μmol/L for ZEN; 0.048–0.065 μmol/L for FB1). These satisfying results demonstrate promising applications for on-site quick testing in practical sample analysis. Moreover, the amino functionalization may also serve as a modification strategy to design luminescent sensors for other food contaminants.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xuanxuan Hao,Zhimin Liu,Yaxin Li,Waner Hou,Tongtong Zheng,Leqian Hu
In this study, a near-infrared (NIR) electrochemiluminescence (ECL) emitter CdIn 2 S 4 was synthesized by a convenient hydrothermal strategy. The ECL properties of the obtained CdIn 2 S 4 were examined in the presence of potassium persulfate coreactant at an excitation potential of − 1.25 V. The low trigger voltage of CdIn 2 S 4 may be ascribed to its lower bandgap. This lower excitation potential could effectively reduce the side-effects induced by high voltages, while maintaining the biological activity of antibodides and antigens. The ECL features of CdIn 2 S 4 were also investigated, demonstrating that CdIn 2 S 4 exhibited excellent NIR characteristics, and surface state transitions led to the ECL emission. Importantly, CdIn 2 S 4 was introduced into ECL and electrochemical impedance spectroscopy (EIS) to establish a dual-mode immunosensing interface. Meanwhile, AuNPs@g-C 3 N 4 with excellent properties were used to promote electron transmission and increase the immobilization amount of zearalenone (ZEA) antibodies in the dual-mode sensing system. The developed immunosensing strategy displayed superior sensing performance in ZEA analysis. The detection limits of ZEA were 30 fg mL −1 (ECL model) and 10 fg mL −1 (EIS model), respectively. The desirable recovery results of ZEA in corn samples prove the application potential of the NIR emitter CdIn 2 S 4 in developing a dual-mode immunosensing system for early real-time use.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yi Zhang,Guoxin Chen,Xirui Chen,Xiaxia Wei,Xuan-ang Shen,Hu Jiang,Xiaoyang Li,Yonghua Xiong,Xiaolin Huang
Herein we developed a multicolor lateral flow immunoassay (LFIA) test strip for rapid and simultaneous quantitative detection of aflatoxin B1 (AFB1) and zearalenone (ZEN). Three differently colored aggregation-induced emission nanoparticles (AIENPs) were designed as LFIA signal tags, with red and green AIENPs for targeting AFB1 and ZEN at the test line, and yellow AIENPs for indicating the validity of the test strip at the control (C) line. After surface functionalization with antibodies, the developed AIENP-based multicolor LFIA allows simultaneous and accurate quantification of AFB1 and ZEN using an independent C-line assisted ratiometric signal output strategy. The detection limits of AFB 1 and ZEN were 6.12 and 26 pg/mL, respectively. The potential of this method for real-world applications was well demonstrated in corn and wheat. Overall, this multicolor LFIA shows great potential for field screening of multiple mycotoxins and can be extended to rapid and simultaneous monitoring of other small molecule targets.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Sohail Mumtaz,Muhammad Imran,Amir Muhammad Afzal
In this study, we develop photoresponsive heterogeneous Cobalt-nickel-based MOFs (CoNi-MOFs), cobalt dioxide (CoO 2 ), and tungsten diselenide (WSe 2 ) hybrid device to improve the performance of photo-assisted supercapacitors. We measure the electrochemical characteristics of photo-assisted hybrid electrodes under both dark and light conditions using cyclic voltammetry and galvanostatic charge–discharge. In dark, a high specific capacity (Qs) is estimated at 970 C/g, increased to 1230 C/g under the light in three-electrode assemblies. An FTO/CoNi-MOF@CoO 2 @WSe 2 /Electrolytes//PANI@AC/GS photo hybrid supercapacitors device is designed and measured in both conditions. The hybrid device showed Qs of 325 C/g in light conditions with energy and power densities of 72 Wh/kg and 827 W/kg, respectively. Even after undergoing 6000 cycles, it exhibited a capacity retention (CR) rate of 91 % and columbic efficiency of 93 %, indicating its exceptional durability as an energy storage device. Besides, the composite devices detect zearalenone in agricultural food samples. The optimal signal for Zearalenone in phosphate buffer solution was observed at pH 7.0, yielding the highest detected response. The sensor demonstrated a broad linear dynamic range of 1–300 μM and an impressively low detection limit of 0.5 nM for Zearalenone concentration. The CoNi-MOF@CoO 2 @WSe 2 nanocomposite has a remarkable sensitivity value of 110.72 mA/cm −2 /mM −1 and response against zearalenone in light. The findings also suggest that the capacitance of the CoNi-MOF@CoO 2 @WSe 2 electrode may increase at an optimal temperature. This likely occurs due to enhanced ion diffusion and charge transfer kinetics, which can improve the energy storage device’s efficiency and aid in identifying the cancerous part of the body.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shanghong Ji,Tingtiao Pan,Weizhen Xu,Song Tang,Guodong Mu,Zeyu Hu,Yu Zhou
Zearalenone (ZEN) poses a substantial threat to global cereal grain security. Herein, a dual-readout biotin-avidin amplified nanozyme-linked immunosorbent assay (BA-ELISA) based on absorbance and blue intensity was established for detecting ZEN residues in corn. The method utilized alkaline phosphatase (ALP), which hydrolyzes ascorbic acid 2-phosphate (AAP) to generate ascorbic acid (AA). The resulting AA reduced K₃[Fe(CN)₆] to K₄[Fe(CN)₆], subsequently reacting with FeCl₃ to form blue Prussian blue nanoparticles (PBNPs). Concurrently, AAP reacted with FeCl₃ to yield a brown AAP-Fe 3+ complex, enabling dual-readout detection via PBNPs and AAP-Fe 3+ . The assay exhibited a linear detection range of 0.625–10 ng/mL, a limit of detection (LOD) of 0.022 ng/mL via absorbance and 0.00447 ng/mL via digital colorimetry, and spiked recovery rates of 104.63 %–118.64 % in corn samples. This dual-readout digital colorimetric detection provided a convenient, sensitive platform for on-site toxin monitoring, offering new perspectives for food safety inspection.
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