Journal of Water Process Engineering
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2023
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IF 7
Jiaqi Wei,Yuanyuan Zhang,Jinxing Ma,Shanshan Wang,Minmin Jiang,Qiaoyan Wei,Mei Chen,Xiaowu Huang,Haixiang Li,Xuehong Zhang,Junjian Zheng
Degradation of refractory organics has been limited by their low mass-transport rates in conventional flow-by operated electrochemical reactors. This study explores for the first time the development of porous titanium (Ti)-based metal oxide anodic membrane for efficient flow-through degradation of recalcitrant tetrabromobisphenol A (TBBPA). For this purpose, a duplexed Ti/SnO 2 -Sb/PbO 2 -Ce anodic membrane was successfully prepared by sol-gel and electrodeposition methods. Ti/SnO 2 -Sb/PbO 2 -Ce exhibited improved mechanical and electrochemical stability, larger electrochemical surface area, higher oxygen evolution potential and lower charge transfer resistance, as compared to its mono-layered rival (Ti/SnO 2 -Sb). The switching from flow-by to flow-through operation resulted in an increased mass transfer rate constant and narrowed diffusion layer thickness of Ti/SnO 2 -Sb/PbO 2 -Ce. In the flow-through mode, Ti/SnO 2 -Sb/PbO 2 -Ce enabled the complete TBBPA removal at a relatively low electric energy (0.11 kWh m −3 ). The TBBPA removal was ascribed to the direct anodic oxidation and indirect anodic oxidation incurred by absorbed HO , free HO and 1 O 2 . The intermediate products, which were arisen from the attack of TBBPA by these reactive species, were identified. We further estimated their acute toxicities and bioaccumulation factors. This research proposed a robust electrochemical filtration approach for detoxification of TBBPA-contaminated water.
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JOURNAL OF APPLIED MICROBIOLOGY
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2023
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IF 4
Tang Xi,Cai Yi-Fan,Yu Xiao-Mei,Zhou Wen-Wen
AimsThis study aimed to screen a bacterial strain with high detoxifying capability for aflatoxin B1 (AFB1), verify its biotransformation efficiency, and detoxification process.Methods and resultsThree hundred and fifty samples collected from different environmental niche were screened using coumarin as the sole carbon source. HPLC was used to detect residues of AFB1, and 16S rRNA sequencing was performed on the isolated strain with highest AFB1 removal ratio for identification. The detoxified products of this strain were tested for toxicity in E. coli as well as LO2, Caco-2 and HaCaT human cell lines. HPLC-MS was applied to further confirm AFB1 removal and detoxification process.ConclusionsWe identified a strain from plant leaf designated as DT with high AFB1-detoxifying ability that is highly homologous to Bacillus aryabhattai. The optimum detoxification conditions of this strain were 37°C and pH 8.0, resulting in 82.92% removal ratio of 2 μg mL−1 AFB1 in 72 h. The detoxified products were nontoxic for E. coli and significantly less toxic for the LO2, Caco-2 and HaCaT human cell lines. HPLC-MS analysis also confirmed the significant drop of the AFB1 characteristic peak. Two possible metabolic products, C19H15O8 (m/z 371) and C19H19O8 (m/z 375), were observed by mass spectrometry. Potential biotransformation pathway was based on the cleavage of double bond in the terminal furan of AFB1. These generated components had different chemical structures with AFB1, manifesting that the attenuation of AFB1 toxicity would be attributed to the destruction of lactone structure of AFB1 during conversion process.
研究领域:
微生物学
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毒理学
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食品安全
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环境科学
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Cell Chemical Biology
·
2025
·
IF 7.2
Wen-Chao Yu,Yan-Lei Yu,Bing-Cheng Dong,Ze-Yu Wang,Au-Qi Du,Song-Wei Li,Buddha Bahadur Basnet,Xiao-Ze Bao,Xuan-Rong Sun,Xing-Nuo Li,Qi Xuan,Qihao Wu,Hong Wang,Bin Wei
Natural products embedded within complex metabolomes are valuable sources of drug leads. Untargeted metabolomics using cosine-based MS 2 comparisons is widely used for discovering bioactive molecules. To improve annotation accuracy and resolution, alternative algorithms have been developed to complement cosine-based MS 2 comparison. Here, we present MSanalyst, a user-friendly platform that integrates 46 distinct mass spectral similarity algorithms. Benchmarking with microbial metabolite datasets and over three million MS 2 spectral pairs demonstrated that complementary algorithms substantially enhance the detection of metabolite-metabolite spectral connections. Applying MSanalyst to Kutzneria viridogrisea DSM 43850 led to the discovery of a class of aromatic glycosides, the kutznaposides. Biological assays and multi-omics analyses revealed that kutznaposides C–F arise from a previously unrecognized menaquinone shunt pathway, enabling the host to mitigate oxidative stress and avoid self-toxicity. Collectively, these findings highlight the potential of MSanalyst to uncover hidden metabolites, metabolic pathways, and their biological functions.
研究领域:
天然产物化学
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代谢组学
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微生物次级代谢
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药物代谢
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分子生物学
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生物活性分子发现
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Xuedan Li,Hang Ma,LingLing Li,Yufeng Gao,Yunzhen Li,Heng Xu
A pot experiment was conducted to investigate the detoxification mechanism of Agrocybe aegerita ( A. aegerita ). The physiological responses , subcellular distribution and chemical forms of cadmium (Cd) in A. aegerita grown in Cd stress were analyzed. The results showed that the biomass was decreased under Cd stress, while the production of malonaldehyde, thiols, and low-molecular-weight organic acids (LWMOAs) as well as the antioxidant enzymes in A. aegerita was increased compared with control group. The HPLC results showed that nine LWMOAs were found in A. aegerita with critic acid as the dominant and they played important role in the detoxification and accumulation of Cd in A. aegerita . More Cd was accumulated in pileus than in stipe. Differential centrifugation technique showed that the majority of Cd was compartmentalized in the soluble fraction (53–75%) and bound to the cell wall (19–42%). The proportion of Cd in the cell wall increased with the increase of the accumulation of Cd in the fruiting body, but in the soluble fraction showed an opposite trend. Furthermore, most of the Cd in A. aegerita was mainly in the forms of NaCl- (29–49%) and ethanol-extractable Cd (20–40%). The ethanol- and water-extractable Cd in stipe (60–66%) was higher than in pileus (43–49%). Thus intracellular detoxification mechanisms of Cd in A. aegerita is related to subcellular partitioning and chemical forms of Cd and well-coordinated physiological responses.
研究领域:
真菌学
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环境毒理学
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植物生理学
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WATER RESEARCH
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2026
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IF 12.4
Sihan Zhang,Jiaxing Lu,Lin-lan Zhuang,Huacheng Xu,Guoqiang Wang,Mingde Ji,Qiang Kong,Zhen Hu,Jian Zhang
Sulfamethoxazole (SMX) present in tailwater from wastewater treatment plants (WWTPs) poses a significant threat to ecosystems. Sulfur-based constructed wetlands (CW-S), employed as post-treatment processes in WWTPs, exhibit inadequate removal efficiency for SMX stemming from inefficient electron transfer. Herein, we added pyrite to CW-S (CW-FeS 2 /S) to stimulate sulfur redox cycling, thereby achieving efficient electron transfer, and explored the underlying mechanisms of SMX removal and detoxification. The results revealed that CW-FeS 2 /S presented the highest total inorganic nitrogen (81.15 ± 5.64%) and SMX (79.02 ± 11.41%) removal, with a 62.77% enhancement in SMX removal relative to CW-S. Pyrite addition enhanced sulfur oxidation-reduction cycles in CW-FeS 2 /S and increased electron transport system activity by 1.94-fold compared to CW-S. Besides, the activation of sulfur-cycling in CW-FeS 2 /S significantly contributed to the breakdown of S−N and N−O bonds, along with microbial-mediated ring-opening reactions, leading to improved mineralization of SMX and a notable reduction in toxicity. Microbial analysis proved that CW-FeS 2 /S enriched Microbacterium and Nocardioides , which were capable of cross-feeding via extracellular electron transfer (EET) mediated by sulfur-cycling to facilitate SMX mineralization. The abundances of sulfur-cycling bacteria and functional genes related to sulfur oxidation and electron transport increased in CW-FeS 2 /S, providing evidence for active sulfur-cycling. Co-occurrence analysis revealed that CW-FeS 2 /S not only increased microbial network complexity and connectivity but also strengthened cooperative interactions among microorganisms. This study supplied a promising technique for simultaneous N and SMX removal from tailwater, and offered insights into microbial mediated sulfur cycle for SMX removal and detoxification mechanisms in constructed wetlands.
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PLOS BIOLOGY
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2026
·Vol. 24
·期 9
·e3004013
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IF 6.9
Huidong Wang,Yajie Kong,Xinyue Su,Yu Shi,Jianpeng Zhang,Yinjia Wang,Yajuan Xiao,Xing Geng,Jing Song,Yueru Ye,Kairan Zuo,Chris Bass,Shutang Zhou
Plants and herbivorous insects are engaged in a continuous evolutionary arms race driven by chemical defences and counter-defences. How insects integrate distinct detoxification systems to overcome diverse phytochemicals, and how such mechanisms contribute to host-range divergence, remains poorly understood. Here, we uncover a cooperative cytochrome P450–UDP-glycosyltransferase (UGT) cascade that mediates furanocoumarin tolerance in Helicoverpa armigera . Comparative genomic analyses across Helicoverpa and related noctuid species revealed that the UGT33 family is the most extensively expanded and dynamically diversified UGT lineage in Helicoverpa . Targeted CRISPR–Cas9 knockouts of UGT33 gene clusters in polyphagous H. armigera demonstrated their essential roles in detoxifying the furanocoumarins xanthotoxin and imperatorin. Combined metabolic and functional assays further established a sequential detoxification pathway, in which the cytochrome P450 CYP6AE19 catalyses either the O-dealkylation of xanthotoxin to yield xanthotoxol or the aromatic-carbon hydroxylation of xanthotoxin to form 5-hydroxyxanthotoxin, which are subsequently glycosylated by UGT33 enzymes to form their less-toxic glucosides. In contrast, impaired CYP6AE19–UGT33 coordination in the oligophagous Helicoverpa assulta was associated with reduced xanthotoxin detoxification and high sensitivity to this compound. Together, these findings provide direct evidence for coordinated Phase I–Phase II detoxification of a plant defensive compound in insects and show that functional divergence in this pathway contributes to interspecific differences in plant toxin tolerance, which are associated with contrasting dietary breadth in closely related herbivorous insects.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2026
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IF 12.2
Fa Liu,Quanyong Zhang,Shu Feng,Xifen Zhu,Shaofu Deng,Yuanyuan Yu,Tao Ai,Yirong Deng,Jianteng Sun,Guanyong Su,Daohui Lin,Lizhong Zhu
Cyano-substituted liquid crystal monomers (cyano-LCMs), widely used in electronic display manufacturing, have emerged as persistent and potentially bioaccumulative contaminants in aquatic environments. However, their microbial transformation mechanisms remain largely unknown. Herein, we report the first characterization of the aerobic microbial biotransformation pathway of 4′-(hexyloxy)-4-biphenylcarbonitrile (6OCB), a representative cyano-LCM, by Sphingopyxis sp. strain ZD-6 isolated from e-waste-contaminated soil. Under aerobic conditions, 92.4% of 6OCB was degraded within 6 days. A total of 13 metabolites were detected, including eight phase II conjugates, indicating a two-step degradation mechanism involving initial oxidative, reductive, and hydrolytic transformations leading to reactive intermediates, followed by conjugation reactions, yielding diverse phase II products, including glutathione-conjugated metabolites. Genomic and transcriptomic analyses identified key enzymes, FAD-dependent oxidoreductases, glutathione S-transferases, and glutamine synthetase, potentially involved in these transformations. Most conjugates showed reduced predicted toxicity, whereas lipid-bound derivatives tended to exhibit higher predicted toxicity. These findings highlight the role of microbial phase II metabolism in cyano-LCM transformation and emphasize the importance of evaluating the environmental fate and potential risk of conjugated metabolites in future risk assessment and bioremediation strategies.
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LANGMUIR
·
2026
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IF 4.4
Yue Qiu,Yingying Ling,Yahong Yuan,Tianli Yue,Fei Lyu
Patulin (PAT), a hazardous mycotoxin found in fruits and their products, poses significant health risks. While UiO-66-NH2 has been primarily explored as an adsorbent or carrier for mycotoxin removal, this study investigates its previously underexplored role as an oxidase-mimicking nanozyme for PAT degradation. UiO-66-NH2 achieved a maximum degradation efficiency of 84.01% within 48 h and 97.63% after 96 h. Mechanism studies revealed that the Zr–O cluster activates O2 to generate reactive oxygen species (·O2– and ·OH), which mediate the oxidative opening of the lactone and furan rings in PAT. The −NH2 enhances both PAT affinity and electron transfer of UiO-66. Three-dimensional fluorescence and LC-MS/MS analyses identified four degradation products (C7H7O5, C7H9O7, C4H5O5, and C3H5O3), enabling the proposal of a plausible degradation pathway. Applied to apple juice, UiO-66-NH2 removed 85.29% of PAT in 72 h, with a negligible impact on juice quality. This work offers an efficient nanozyme-based strategy for PAT detoxification in food applications.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
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IF 4
Rui Chu,Wenhui Li,Jintian Wang,Xiaoyang Li,Tianying Lu
BACKGROUND Patulin (PAT) is a mycotoxin commonly found in apple products, which poses significant health risks and is challenging to remove using conventional methods. This study employed a ligand functionalization strategy to precisely modulate the microenvironment of metal–organic frameworks and investigate its impact on PAT adsorption performance. RESULTS A series of functionalized UiO-66 nanomaterials (UiO-66, UiO-66-NH 2 , and UiO-66-SH) were synthesized via solvothermal method using zirconium as the metal node and terephthalic acid derivatives as organic linkers. Comprehensive characterization confirmed the successful incorporation of the corresponding functional groups and the conserved crystalline structure of the UiO-66. Screening of their adsorption capabilities revealed a dramatic dependence on the surface chemistry, with UiO-66-SH exhibiting superior PAT adsorption performance and achieving complete removal under optimized conditions. Adsorption kinetics and isotherm studies were conducted. The results demonstrated that the adsorption process of UiO-66-SH toward PAT followed a pseudo-second-order kinetics model and the Langmuir adsorption isotherm, indicative of chemisorption-driven monolayer adsorption. The maximum adsorption capacity was determined to be 18.209 μg mg −1 . Mechanistic investigations confirmed that the thiol groups on UiO-66-SH chemically interacted with PAT via a Michael addition reaction, thereby achieving efficient adsorption and removal of PAT. In practical application, UiO-66-SH effectively removed PAT from spiked apple juice while maintaining the key quality parameters of apple juice. CONCLUSION Our study demonstrates that ligand-induced microenvironment modulation renders UiO-66-SH a highly efficient and selective adsorbent for PAT decontamination, offering a promising strategy for enhancing the safety of apple products. © 2026 Society of Chemical Industry.
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ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Xiao-Qian Jin,Jia-Lei Li,Jun-Bin Xiong,Rui Zhang,Chen-Yuan Li,Xue-Ting Wang,Wei-Hong Guo,Da-Chuan Yin
In this work, cross-linked hemoglobin crystals (CLHCs) were synthesized using discarded cow blood as raw material, and palladium nano particles (Pd NPs) was synthesized in situ within its lattice to obtain CLHCs@Pd catalytic used for catalyzing the reduction of Cr(VI). In addition, we investigated the influence of synthesis process on the morphology, loading capacity, and size of Pd NPs, and the results showed that the minimum size of Pd NPs was distributed around 2 nm under any process conditions. The X-ray single crystal diffraction results indicate that the lattice of protein crystals may have a controlling effect on the size of Pd NPs. Subsequently, the mechanism of synthesizing Pd NPs by CLHCs was studied using techniques such as SEM, TEM, FTIR and XPS. Finally, the catalytic activity and cycling performance of Cr(VI) removal by CLHCs@Pd were evaluated. The results indicate that the CLHCs@Pd not only exhibit high catalytic activity for Cr(VI) removal, but also maintain consistent catalytic performance over 20 consecutive catalytic cycles. Overall, this work provides CLHCs@Pd catalysts have the following advantages: (1) small size and uniform dispersion of Pd NPs; (2) High cycling performance and high catalytic activity; (3) Low cost and environmentally friendly preparation process.
研究领域:
环境科学
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材料科学
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纳米技术
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Journal of Environmental Sciences
·
2019
·
IF 3.556
Shijin Dai,Yilin Zheng,Youcai Zhao,Yu Chen,Dongjie Niu
Hazardous waste dechlorination reduces the potential of creating dioxins during the incineration process. To investigate the salt effect on waste dechlorination, molten hydroxides with a low melting temperature were utilized for the pre-dechlorination and decomposition of chlorine-containing organic wastes (COWs) including trichlorobenzene (TCB), perchloroethylene , hexachlorobenzene and chlordane . The results showed that a eutectic mixture of caustic sodium and potassium hydroxides (41 wt.% NaOH and 59 wt.% KOH) led to a low melting point below 300°C and a relatively high chlorine retention efficiency (CRE) with TCB as a representative COWs. The amounts of hydroxides, reaction time, and temperature all had notable influence on CRE. When the mass ratio of hydroxides to TCB reached 30:1, approximately 98.1% of the TCB was destroyed within 2.5 hr at 300°C with CRE of 71.6%. According to the residue analysis , the shapes of reaction residues were irregular with particles becoming swollen and porous. The benzene ring and C–Cl bonds disappeared, while carboxyl groups formed in the residues. The stripped chlorine was retained and condensed to form chloride salts, and the relative abundance of the chloride ions associated with the mass of TCB in residues increased from 0 to 75.0% within the 2.5 hr reaction time. The observed concentration of dioxins in residues was 5.6 ngTEQ/kg. A reaction pathway and possible additional reactions that occur in this dechlorination system were proposed. Oxidizing agents may attack TCB and facilitate hydrogenation/dechlorination reactions, making this process a promising and environmentally friendly approach for chlorine-containing organic waste treatment .
研究领域:
环境科学
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化学工程
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废物管理
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Wenjun Xiong,Wei Wei,Man He,Bin Hu,Jun Men,Jiawei Tu,Wei Miao
Biomethylation is an effective means of arsenic detoxification by organisms living in aquatic environments. Ciliated protozoa (including Tetrahymena species) play an important role in the biochemical cycles of aquatic ecosystems and have a potential application in arsenic biotransformation. This study compared arsenic tolerance, accumulation, methylation, and efflux in 11 Tetrahymena species. Nineteen arsenite (As(III)) S -adenosylmethionine (SAM) methyltransferase ( arsM ) genes, of which 12 are new discoveries, were identified, and protein sequences were studied. We then constructed recombinant cell lines based on the Tetrahymena thermophila ( T. thermophila ) wild-type SB210 strain and expressed each of the 19 arsM genes under the control of the metal-responsive the MTT1 promoter. In the presence of Cd 2+ and As(V), expression of the arsM genes in the recombinant cell lines was much higher than in the donor species. Evaluation of the recombinant cell line identified one with ultra-high arsenic methylation enzyme activity, significantly higher arsenic methylation capacity and much faster methylation rate than other reported arsenic methylated organisms, which methylated 89% of arsenic within 6.5 h. It also had an excellent capacity for the arsenic detoxification of lake water containing As(V), 56% of arsenic was methylated at 250 μg/L As(V) in 48 h. This study has made a significant contribution to our knowledge on arsenic metabolism in protozoa and demonstrates the great potential to use Tetrahymena species in the arsenic biotransformation of aquatic environments.
研究领域:
环境生物学
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分子生物学
·
生态学
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危险品化学品经营许可证（带存储）