STAR Protocols
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2023
·
IF 0
Zhen-Ping Zou,Yu-Hang Fan,Yue Du,Ting-Ting Fang,Wei Du,Ying Zhou,Bang-Ce Ye
Summary Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for constructing and administering iROBOT, diagnosing and recording colitis, preparing samples, and analyzing fluorescence and base editing ratios of iROBOT. We detail a colitis ameliorating assay using the disease activity index, colon length, tissue pathological section, and cytokine analysis. For complete details of the use and execution of this protocol, please refer to Zou et al. 1
研究领域:
微生物学
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分子生物学
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基因工程
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炎症性肠病
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DOI
PubMed
JOURNAL OF CHROMATOGRAPHY A
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2025
·
IF 4
Mengting Cui,Qingpeng Gao,Jinling Bai,Mengting Fu,Zhiyu Liu,Zhixin Ouyang,Liyun Kong,Le Ma
Conventional extraction techniques for fat-soluble nutrient analysis in foods may present certain limitations, including variable or limited efficiency, high solvent consumption, lengthy procedures, and potential environmental and health concerns. This study evaluates deep eutectic solvents (DESs) as a green and efficient alternative for extracting vitamin E and carotenoids from vegetables and fruits. Conductor-like screening model for real solvents (COSMO-RS) simulation and experimental screening identified the menthol-thymol (1:2) DES as optimal due to the balanced distribution of polar and nonpolar sites within its components. A novel dispersive liquid-liquid microextraction method based on this DES (DES-DLLME) was developed and integrated with HPLC for the simultaneous quantitation of vitamin E and carotenoids. The optimized method demonstrated excellent analytical performance with low detection limits (0.01-0.18 µg/mL), satisfactory recovery rates (80.04-111.95%), and high reproducibility (relative standard deviation, RSD < 16.83%). When applied to a range of Chinese food samples, the obtained nutrient profiles were largely consistent with USDA reference data, with observed variations likely due to factors such as geographic origin, cultivation practices, and post-harvest processing. This DES-based approach offers an efficient, reproducible, and environmentally friendly method for determining fat-soluble vitamins and carotenoids in complex food matrices, offering significant value for food quality assessment and nutritional research.
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DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Ping Lu,Zixing Gu,Zihan Zhang,Huaneng Su,Qiang Ma,Chunsheng Li,Lei Wei,Qian Xu
The all-in-one E-fueled solar flow battery system that can be recharged with liquid fuel is a new type of power system. It can continuously convert solar energy into liquid fuel without relying on external power supply equipments. However, the interface between the photoanode and the electrolyte often exhibits unsteady behavior, resulting in poor performance of the system. In this work, the {201} facets are selected and the impact of two doping treatment strategies for TiO 2 {201} photoanode on the performance of the E-fueled solar flow battery system is systematically studied through the I-t curve, linear sweep voltammetry, Mott-Schottky plot. The results show that the un-biased photocharging current of bulk-doped TiO 2 can reach 2.7 mA·cm −2 in the battery system test, only relying on the self-built electric field at the interface, and the energy conversion efficiency approaches 2.67 %. In addition, the performance is analyzed by the combination of transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and other characterizations, with the aim of distinguishing the effects of bulk- and surface-doped Mn and accurately revealing the mechanism of their influences from the outside to the inside. Finally, the optimization rules of the un-biased light charging mode of the system are explored.
研究领域:
材料科学
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电化学
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太阳能转化
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电池技术
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DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Rui Hong,Ri-Tong Lan,Yue Ren,Lu Xu,Yun-Xiang Xu,Nouman Ali Shah,Rizwan M. Gul,Shishu Huang,Lingli Li,Jia-Zhuang Xu,Zhong-Ming Li,Ka Li
Oxidation resistance is strongly necessary for the use of crosslinked ultrahigh molecular weight polyethylene (UHMWPE) in artificial joints. The hybrid antioxidant strategy emerged as a promising alternative to the single rival that relies on vitamin E (VE). The goal of this study was to investigate the universality of the antioxidant synergy between VE and sugar alcohol (SA). Three types of SA, including D-sorbitol, maltitol and erythritol, were employed to cooperate with VE. Compared to the use of VE alone, these hybrid antioxidants generally enabled the corresponding UHMWPE composites with prolonged oxidation induction time (OIT) and increased crosslink density. The correlation between OIT and crosslink density of VE/SA-stabilized UHMWPE composites showed low attenuation. The applicability of the hybrid antioxidants to protect UHMWPE against oxidation was further demonstrated using the in vitro clinically relevant accelerated aging. Among the used SA, D-sorbitol exhibited the strongest ability to enhance the synergetic efficiency. The possible mechanism was revealed from the following aspects: the number of secondary hydroxyls per molecular mass and the molecular polarity determined via polarity parameter calculation. These findings provide significant insights for fabricating UHMWPE bearing materials with superior comprehensive performance and extended service longevity.
研究领域:
材料科学
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生物医学工程
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高分子化学
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DOI
高影响因子
AAPS PHARMSCITECH
·
2020
·Vol. 21
·期 5
·1-13
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IF 2.401
Liu Dandan,Qiao Sen,Cheng Bingchao,Li Dongyang,Chen Jisuan,Wu Qingyin,Pan Hao,Pan Weisan
Despite that either non-covalent or covalent attachment of hydrophilic polymers or surfactants onto nanodiamonds (NDs) could overcome the shortcomings of being a drug delivery system, it is hard to draw a definite conclusion which strategy is more effective. Hence, with the purpose of comparing the influence of different coating approach of NDs on the oral delivery efficiency of water-insoluble model drug curcumin (CUR), NDs were firstly modified with d -α-tocopheryl polyethylene glycol 1000 succinate (TPGS) via non-covalent or covalent conjugation method, and then loaded with CUR (CUR@NDs-COOH/TPGS or CUR@NDs-TPGS). In comparison with the core-shell-structured CUR@NDs-COOH/TPGS, CUR@NDs-TPGS were irregular in shape with dense TPGS film, and exhibited smaller size, more negatively potential, and higher drug loading efficiency. The covalent connection group also showed higher anti-cancer activity, cellular uptake, and permeability through the Caco-2 cell monolayers, as well as favorable distribution, penetration, and retention in rat intestines. The oral bioavailability study in rats demonstrated that CUR@NDs-TPGS showed significantly greater C max and AUC 0− t in contrast with CUR suspension and the TPGS-coated ones, respectively. The findings illustrated that covalent grafting TPGS onto the surface of NDs possesses better efficacy and biocompatibility on oral delivery of poorly soluble drug CUR than pristine and non-covalent coated nanoparticles.
研究领域:
药学
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药物递送系统
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纳米技术
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药代动力学
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DOI
PubMed
高影响因子
WASTE MANAGEMENT
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2025
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IF 7.1
Ke Xu,Shengde Dong,Chunxi Hai,Luxiang Ma,Yanxia Sun,Qi Xu,Xin He,Yuan Zhou
The recycling of cathode materials in waste lithium-ion batteries is of great significance to resource utilization and environmental protection. However, few studies have addressed the challenges posed by high-aluminum LiFePO 4 black powder (HALFP-BP) resulting from inadequate pre-treatment. This study proposes a low-temperature short-time sulfuric acid leaching method based on thermodynamic analysis of E-pH diagrams. Under optimized conditions, the leaching efficiencies of Li and Fe reached 99 %, while 86 % of Al was selectively removed. Furthermore, the guiding role of E-pH diagram in the recovery process and the chemical reactions and Gibbs free energy analysis during the acid leaching process were analyzed. It revealed that the dissolution kinetics inhibition of the dense Al 2 O 3 film on the surface of aluminum impurities in the sulfuric acid system was the key to achieving selective separation. This method provides an energy-saving and efficient solution for the resource utilization of the HALFP-BP.
研究领域:
冶金学
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环境科学
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化学工程
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材料科学
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回收技术
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2025
·
IF 8.2
Pengjie Zhong,Youqiang Huang,Qinglong Hu,Xin Yao,Wei Liu,Keyu Han,Gongxun Bai
Solar-driven interfacial evaporation has garnered significant attention as a sustainable technology for freshwater production. However, its widespread application is impeded by challenges, such as complex fabrication processes, high costs, and vulnerability to pore clogging. Herein, we present an E-shaped evaporator constructed through in situ integration of a binary photothermal nanocomposite comprising reduced graphene oxide and molybdenum disulfide (rGO/MoS2) onto a polyurethane sponge substrate. The synergistic light absorption enhancement of the rGO/MoS2 hybrid is rationally utilized to achieve excellent broadband solar harvesting. The distinctive E-shaped configuration simultaneously optimizes light utilization through multiangle photon capture and facilitates rapid water transport via hierarchical channels. This integrated system demonstrates exceptional operational stability with a cumulative water yield of 16.58 kg·m–2 under 12 h natural illumination, which could effectively facilitate seawater desalination and wastewater purification. The proposed fabrication strategy, characterized by cost-effectiveness and scalable manufacturing compatibility, paves the way for practical implementation in off-grid water purification systems.
研究领域:
可再生能源
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材料科学
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环境工程
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纳米技术
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水处理技术
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Qian Zhang,Xudong Lü,Yuanyuan Jin,Mengjia Tang,Miao Wang,Sen Zou,Shuai Fan,Zhaoyong Yang
Cyst(e)inase, an engineered human cystathionine-γ-lyase (hCGL), effectively degrades l-Cys and CSSC, inhibiting various tumors in vivo with minimal toxicity. However, its current activity and stability limit its clinical application. To enhance its therapeutic potential, we utilized folding free energy calculations and amino acid site conservation to identify 46 potential mutants. Among them, 17 mutants exhibited improved activity or stability, while six showed a loss of function. Notably, mutant L142E enhanced activity for both substrates, and E349S displayed 2.8 times higher activity toward CSSC and a 12.8 °C increase in Tm, despite reduced activity toward l-Cys. Saturation mutagenesis highlighted the importance of spatial constraints for substrate selectivity. MD simulations suggested that distal mutations stabilize the near-attack conformation, enhancing the activity. In vitro cytotoxicity assays confirmed that the mutants L142E and E349S significantly inhibited gastric and pancreatic cancer cells. This study provides valuable insights for improving therapeutic enzyme design.
研究领域:
生物化学
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结构生物学
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计算生物学
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癌症治疗
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酶工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Ziyuan Zheng,Wenguang Yan,Wenlong Liu,Zihao Cai,Yanjun Ding,Jiang Ling
Volatile organic compounds (VOCs) play a critical role in human physiology, with their concentrations serving as key indicators of physiological states. Among these, trans-4,5-epoxy-(E)-2-decenal, a volatile organic compound found in mammalian blood, exhibits unique chemical reactivity and olfactory sensitivity due to its molecular structure, which is closely associated with lipid peroxidation processes. However, conventional detection methods, such as gas chromatography-olfactometry-mass spectrometry (GC-O-MS), suffer from limitations including inherent subjectivity, poor reproducibility, stringent operational requirements, and low throughput, which undermine their clinical reliability. To address these challenges, this study developed an optimized liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for accurate qualitative and quantitative analysis. Given the poor detectability of aldehydes via LC-MS/MS, 2,4-dinitrophenylhydrazine (DNPH) derivatization was employed to enhance the chromatographic behavior and retention of the target compound. Additionally, HLB solid-phase extraction (SPE) columns was employed for purification to enhance the sensitivity of LC-MS/MS for the detection of trans-4,5-epoxy-(E)-2-decenal. Following derivatization, trans-4,5-epoxy-(E)-2-decenal demonstrated strong retention during chromatographic separation. The mobile phase, composed of acetonitrile and ultrapure water, exhibited significantly superior performance compared to combinations of acetonitrile with ammonium acetate buffer. Under optimized conditions, the LC-MS/MS method demonstrated excellent linearity for trans-4,5-epoxy-(E)-2-decenal within a range of 1–300 ng/mL, with correlation coefficient (R 2 ) of 0.9984, detection limit (LOD) of 0.3 ng/mL. The relative standard deviation (RSD) for spiked blood samples in the experiment was 12.53 %.
研究领域:
分析化学
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生物化学
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医学诊断
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代谢组学
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质谱分析
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高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2013
·Vol. 27
·期 3
·148-154
·
IF 2.011
Li Qiang Wu
Nano n -propylsulfonated γ-Al 2 O 3 is easily prepared by the reaction of nano γ-Al 2 O 3 with 1,3-propanesultone. This reagent can be used as an efficient catalyst for the synthesis of spiro [indoline-3,4-pyrazolo[3,4- e ][1,4]thiazepine]diones in aqueous media. This new method consistently has the advantages of excellent yields and short reaction times. Further, the catalyst can be reused and recovered several times. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
催化化学
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有机合成
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绿色化学
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纳米材料
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药物化学
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 14
·147520
·
IF 3.721
Xinyi Wang,Liubing Kong,Shuqi Zhou,Tao Liang,Hao Wan,Ping Wang
Photoluminescence (PL) sensors based on quantum dots (QDs) are difficult to achieve exact detection in sample waters. In this paper, polyvinyl alcohol (PVA) based CdTe/ZnS/CdS QDs and fluorescein isothiocyanate (FITC) fluorophores were used to build a ratiometric PL sensor. Combining with the homemade smartphone-based PL E-eye, the Cd2+ exact detection can be achieved. PVA was used to connect QDs and FITC fluorophores without unnecessary ligand exchange and purification. For QDs fluorophore, ethylenediaminetetraaceticacid (EDTA) was used on the surface to induce the specifically Cd2+ recognition site. For FITC fluorophore, the PL remains unchanged in this experiment. Thus, the ratio of two fluorophores can be used to provide a built-in correction. The PL changes with the increase of Cd2+ concentrations could be displayed as a visual color change on the smartphone. Further quantitative analysis could be carried out by the RGB value of the picture through the App in less than 1 min The ratio of R/G is linear to Cd2+ concentration in the range of 1–2000 μg l−1 with a low LOD of 0.057 μg l−1 (S/N). Compared with traditional analysis methods, the PL ratiometric method with PL E-eye is portable, rapid, visible and highly selective especially in discriminate Cd2+ from Zn2+.
研究领域:
环境科学
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分析化学
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纳米技术
·
光学传感
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DOI
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Yonggui Song,Meixizi Lai,Zhou Liao,Zhentao Zhang,Genhua Zhu,Ming Yang,Zhifu Ai,Qin Zheng,Dan Su
Background Since the pathogenesis of depression is complex, antidepressant therapy remains unsatisfactory. Recent evidence suggests a link between depression and lipid metabolism. Saikosaponin (SS) exhibits antidepression and lipid-regulating effects in modern pharmacology. However, it is unknown whether lipid regulation is the key mechanism of the SS antidepressant effect and how it works. Purpose In this study, we investigated the relationship between the antidepressant activity of SS and the regulation of lipid metabolism and explored potential mechanisms. Methods APOE -/- mice, in combination with the chronic unpredictable mild stress (CUMS) model, were used to study the relationship between SS antidepressant activity and lipid metabolism through behavioral, electrophysiological techniques, and non-targeted lipidomics. Western blot, primary cell culture technology, and laser speckle cerebral blood flow imaging were employed to elucidate potential mechanisms. GraphPad Prism was used for statistical analysis, and p < 0.05 was considered statistically significant. Results APOE -/- mice exhibit more severe depressive-like behavior and dysregulation of sphingolipid metabolism in CUMS. SS alleviates depressive behavior and cortical sphingolipid metabolism disorder caused by CUMS, but has no effect on APOE -/- mice. SS alleviates the imbalance between ceramide (Cer) and sphingomyelin (SM) through acidic sphingomyelinase (AMSase). In addition, SS regulates neuronal glutamate release via sphingolipid metabolism, thereby alleviating the CUMS-induced inhibition of neurovascular coupling (regulates metabotropic glutamate receptor and IP3 receptor), which ameliorates the reduction of cerebral blood flow in depressed mice. Conclusion Our study highlights the role of lipid metabolism in the antidepressant activity of SS and explores its underlying mechanisms. This study provided new insights into the better understanding of the antidepressant mechanisms of phytomedicine while increasing the possibility of lipid metabolism as a therapeutic strategy for depression.
研究领域:
药理学
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神经科学
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脂质生物学
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行为科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）