JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2009
·
IF 2.562
Zhao-Feng Li,Bin Li,Zhi-Guo Liu,Miao Wang,Zheng-Biao Gu,Guo-Cheng Du,Jing Wu,Jian Chen
Overexpression of recombinant genes in Escherichia coli and targeting recombinant proteins to the culture medium are highly desirable for the production of industrial enzymes. However, a major barrier is inadequate secretion of recombinant protein across the two membranes of E. coli cells. In the present study, we have attempted to circumvent this secretion problem of the recombinant α-cyclodextrin glycosyltransferase (α-CGTase) from Paenibacillus macerans strain JFB05-01. It was found that glycine, as a medium supplement, could enhance the extracellular secretion of recombinant α-CGTase in E. coli. In the culture with glycine at the optimal concentration of 150 mM, the α-CGTase activity in the culture medium reached 23.5 U/mL at 40 h of culture, which was 11-fold higher than that of the culture in regular TB medium. A 2.3-fold increase in the maximum extracellular productivity of recombinant α-CGTase was also observed. However, further analysis indicated that glycine supplementation exerted impaired cell growth as demonstrated by reduced cell number and viability, increased cell lysis, and damaged cell morphology, which prevented further improvement in overall enzyme productivity. Significantly, Ca2+ could remedy cell growth inhibition induced by glycine, thereby leading to further increase in the glycine-enhanced extracellular secretion of recombinant α-CGTase. In the culture with 150 mM glycine and 20 mM Ca2+, both extracellular activity and maximum productivity of recombinant enzyme were 1.5-fold higher than those in the culture with glycine alone. To the best of our knowledge, this is the first article about the synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of a recombinant protein in E. coli.
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JOURNAL OF POLYMER RESEARCH
·
2024
·Vol. 31
·期 11
·1-15
·
IF 2.6
Ni Ruixuan,Yang Ziteng,Wang Li,Dai Shanshan,Lu Hongsheng
Hydrogel as a temporary plugging material provides an efficient strategy to replace locally damaged oil pipelines. However, the poor mechanical properties and incomplete gel-breaking severely limit the practical application of this strategy. Herein, a temperature and pH-responsive hydrogel P(ACG-co-AM)-MMT was prepared by copolymerizing a natural amino acid derivative monomer N-acryloyl glycine (ACG) and acrylamide (AM) with montmorillonite (MMT) as filling material. Hydrogen bonding is the main driving force in hydrogel formation, as confirmed by infrared spectroscopy. The introduction of MMT plays a key role in enhancing the mechanical properties of hydrogels. This successfully overcame the issues of inadequate mechanical properties-rapid degradation trade-off in hydrogel temporary plugging materials. The results show that the hydrogel can withstand the pipeline pressure up to 84 kPa and effectively plug the pipeline. Due to the reversibility of hydrogen bonds, the hydrogel can realize the transformation of sol-gel-sol under temperature stimulation. The plugged area of the pipeline is removed by injecting thermal-produced water. When the hot water temperature is 85 ℃, the pipeline changes to the dredging state only 21 min, dramatically simplifying the operation time. P(ACG-co-AM)-MMT hydrogel has great potential as a temporary plugging material in replacing partially damaged oil pipeline plugging operations. Graphical
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CHEMOSPHERE
·
2024
·
IF 8.1
Chunli Yu,Yao Hu,Yuqin Zhang,Wei Luo,Jing Zhang,Peilun Xu,Jun Qian,Jun Li,Jianfeng Yu,Jin Liu,Wenguang Zhou,Shengxi Shao
In industrial-scale cultivation of microalgae, salinity stress often stimulates high-value metabolites production but decreases biomass yield. In this research, we present an extraordinary response of Arthrospira platensis to salinity stress. Specifically, we observed a significant increase in both biomass production (2.58 g L −1 ) and phycocyanin (PC) content (22.31%), which were enhanced by 1.26-fold and 2.62-fold, respectively, compared to the control, upon exposure to exogenous glycine betaine (GB). The biochemical analysis reveals a significant enhancement in carbonic anhydrase activity and chlorophyll a level, concurrent with reductions in carbohydrate content and reactive oxygen species (ROS) levels. Further, transcriptomic profiling indicates a downregulation of genes associated with the tricarboxylic acid (TCA) cycle and an upregulation of genes linked to nitrogen assimilation, hinting at a rebalanced carbon/nitrogen metabolism favoring PC accumulation. This work thus presents a promising strategy for simultaneous enhancement of biomass production and PC content in A. platensis and expands our understanding of PC biosynthesis and salinity stress responses in A. platensis .
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Journal of Biological Engineering
·
2020
·Vol. 14
·期 1
·1-10
·
IF 3.429
Xu Yingying,Meng Hao,Ren Jie,Zeng An-Ping
Glycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO2 via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its formation. First, the principal one is the decomposition of N5,N10-methylene-tetrahydrofolate (5,10-CH2-THF) to form formaldehyde and THF. Increasing the rate of glycine cleavage promotes the formation of 5,10-CH2-THF, thereby increasing the formaldehyde release rate. Next, formaldehyde can be produced in the GCS even in the absence of THF. The reason is that T-protein of the GCS can degrade methylamine-loaded H-protein (Hint) to formaldehyde and ammonia, accompanied with the formation of dihydrolipoyl H-protein (Hred), but the reaction rate is less than 0.16% of that in the presence of THF. Increasing T-protein concentration can speed up the release rate of formaldehyde by Hint. Finally, a certain amount of formaldehyde can be formed in the GCS due to oxidative degradation of THF. Based on a formaldehyde-dependent aldolase, we elaborated a glycine-based one carbon metabolic pathway for the biosynthesis of 1,3-propanediol (1,3-PDO) in vitro. This work provides quantitative data and mechanistic understanding of formaldehyde formation in the GCS and a new biosynthetic pathway of 1,3-PDO.
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Congming Ke,Shoulin Liu,Yiao Pang,Yongping Wei,Yaping Wu,Qiufa Luo,Yueqin Wu,Jing Lu
With the rapid development of the third-generation semiconductor materials, the chemical mechanical polishing rate and surface quality of single-crystal gallium nitride (GaN) substrates have been a research hot-spot. In this work, the synergistic effect of abrasive friction and glycine (Gly) on improving the chemical mechanical polishing (CMP) performance of single-crystal GaN substrate is proposed and studied systematically. The results indicated that the polishing solutions with Gly have a 2.3-fold improvement in material removal rate (MRR) as well as scratch-free surface than that without Gly. The maximum MRR of the single-crystal GaN substrate is 129.4 nm/h with surface roughness (Ra) of 0.64 nm attained by using the polishing solutions containing only 3 wt% potassium persulfate (K 2 S 2 O 8 ). In contrast, when Gly is added as a reaction reagent, which can be activated by abrasive friction, the maximum MRR is increased to 304.2 nm/h with Ra of 0.42 nm. Meanwhile, the epitaxial evaluation indicates that two-dimensional (2D) MoS 2 grown on processed single-crystal GaN substrate has superior crystalline quality and optical properties. Finally, the polishing mechanism of the single-crystal GaN substrate using the mixed solutions of K 2 S 2 O 8 and Gly is discussed. The proposed CMP method has potential applications in the semiconductor and microelectronics industries.
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JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Changzeng Wu,Jianji Wang,Zhiyong Li,Jun Jing,Huiyong Wang
In this work, glycine ionic liquids tetramethylammonium glycine ([N 1111 ][Gly]), tetraethylammonium glycine ([N 2222 ][Gly]), tetra-n-butylammonium glycine ([N 4444 ][Gly]), tetra-n-butylphosphonium glycine ([P 4444 ][Gly]) and tetra-n-pentylammonium glycine ([N 5555 ][Gly]) were synthesized and used to prepare aqueous two-phase systems (ATPSs) in the presence of K 2 HPO 4 . Binodal curves of such ATPSs and partition coefficients of a series of dinitrophenylated (DNP) amino acids in these ATPSs were determined at 298.15 K to understand the effect of cationic structure of the ionic liquids on the phase-forming ability of glycine ionic liquids, relative hydrophobicity between the phases in the ionic liquids ATPSs, and polarity of the ionic liquids-rich phases. With the attempt to correlate the relative hydrophobicity of the phases in the ATPSs with their extraction capability for proteins, partition coefficients of cytochrome-c in the ATPSs were also determined. It was shown that partition coefficients of cytochrome-c were in the range from 2.83 to 20.7 under the studied pH conditions. Then, hydrophobic interactions between cytochrome-c and the ionic liquid are suggested to be the main driving force for the preferential partition of cytochrome-c in the glycine ionic liquid-rich phases of the ATPSs. Result derived from polarity of the ionic liquids-rich phases supports this mechanism.
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FOOD CHEMISTRY
·
2011
·Vol. 128
·期 2
·442-449
·
IF 3.458
Jie Liu,Fang Chen,Yong Man,Jing Dong,Xiaosong Hu
The reaction between acrylamide and glycine was studied in the aqueous model system heated at 150 °C. The main reaction products were identified as C 5 H 10 N 2 O 3 , C 8 H 15 N 3 O 4 , C 7 H 12 N 2 O 5 and C 10 H 17 N 3 O 6 using HPLC-MS/MS, IT-TOF and NMR. Both of the critical intermediates were identified as glyoxylic acid and iminodiacetic acid. The pathways for the removal of acrylamide by glycine were proposed as the Michael addition between acrylamide and glycine with or without the initial oxidation of glycine. The changes in the contents of reactants and products provided quantitative evidence for the above pathways. The addition products between acrylamide and other 14 amino acids were identified by HPLC-MS/MS also.
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BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2013
·Vol. 17
·期 6
·1128-1134
·
IF 1.277
Li Bin,Wang Lei,Su Lingqia,Chen Sheng,Li Zhaofeng,Chen Jian,Wu Jing
OmpA signal peptide mediated cgt gene from Paenibacillus macerans JFB05-01 was cloned and expressed in E. coli BL21 (DE3). The effects of glycine and Triton X-100 on extracellular production of α-cyclodextrin glycosyltransferase (α-CGTase) were investigated. When supplemented with Gly or Triton X-100 to the culture media individually, the secreted extracellular enzyme reached 32 or 33 U/mL at 48 h of cultivation, respectively. When supplemented with Gly and Triton X-100 together, the extracellular α-CGTase activity reached 48 U/mL after 48 h cultivation, which was 20-fold of the control group without any additives. Analysis of membrane permeability demonstrated that addition of glycine and Triton X-100 enhanced the permeability of both outer and inner membrane. The potential mechanism of the enhanced protein secretion was discussed.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·
IF 12.2
Zongyang Ya,Dong Xu,Xiaohui Tang,Yu Zheng,Zixuan Zhang,Mei Li,Hua Wang,Shengbo Zhang
Photocatalytic conversion of polyols and nitrogen-containing small molecules into value-added chemicals via C–N coupling reaction represents a promising route for dual waste treatment of waste plastics and wastewater. However, precisely controlling the coupling pathway of key intermediates to achieve high product selectivity remains a significant challenge. Here, we report a WO3 photocatalyst that enables the highly selective synthesis of glycine from waste poly(ethylene terephthalate) (PET)-derived ethylene glycol (EG) and nitrate, achieving a remarkable glycine yield of 1.87 mmol gcat–1 h–1 with 89% selectivity, which significantly outperforms conventional TiO2-based systems. Mechanistic studies indicate that the high selectivity stems from the distinct reaction pathway on WO3, where glycolaldehyde is stabilized as the key intermediate to efficient C–N coupling with NH4+ derived from NO3– reduction. This contrasts with the TiO2-catalyzed pathway that proceeds via the more oxidized glyoxal, which readily undergoes overoxidation to the byproduct formic acid. By using different plastic/biomass-derived feedstocks (methanol, ethylene glycol, and glycerol), this process can be expanded to the scale synthesis of various C–N compounds. Preliminary techno-economic analysis and life-cycle assessments suggest the potential economic viability and environmental benefits of this process. This work establishes a sustainable photocatalytic strategy for the valorization of waste plastic and wastewater by regulating key intermediates to enable a selective C–N coupling pathway.
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Biomaterials Science
·
2023
·
IF 6.6
Tingting Zhao,Ting Shu,Jinrong Lang,Ziyu Cui,Ping Li,Shi Wang
Nitric oxide (NO) is a crucial cell-signaling molecule utilized in numerous physiological and pathological processes. Monitoring cellular levels of NO requires a sensor with sufficient sensitivity, transient recording capability, and biocompatibility. Owing to the large surface area and high catalytic activity of the metal–organic framework, Fe–BTC was used for the modification of screen-printed electrodes (SPEs). This study investigates the electrochemical sensing of NO on modified SPEs. Additionally, the introduction of a cell-adhesive molecule, arginine–glycine–aspartate peptide (RGD), considerably improved the cytocompatibility, resulting in superior cell attachment and growth on the SPE. The Fe–BTC/RGD-modified SPE (Fe–BTC/RGD/SPE) exhibited electrocatalytic NO oxidation at 0.8 V, demonstrating a linear response with a detection limit of 11.88 nM over a wide concentration range (0.17–47.37 μM) and a response time of approximately 0.9 s. Subsequently, the as-obtained Fe–BTC/RGD/SPE was successfully utilized for the real-time detection of NO released from human endothelial cells cultured on the electrode. Therefore, the study undertaken shows remarkable potential of Fe–BTC/RGD/SPE for practical applications in biological processes and clinical diagnostics.
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Journal of Energy Storage
·
2023
·
IF 9.4
Yali Liu,Ming Li,Ying Zhang,Yunfeng Wang,Qiongfen Yu,Zhenhua Gu,Runsheng Tang
Organic water-based phase change material (PCM) shows great potential for cold chain logistics below 0 °C. However, its poor stability limits its application. The purpose of this study is to develop an organic water-based PCM with high latent heat and melting temperature of −4 °C to −6 °C based on solving the problem of poor stability. Glycine and mannitol aqueous solution were selected as the base PCM, and the static stability was improved effectively with 0.1 wt% potassium sorbate modification. The prepared 6-PCM5 composed of 6 wt% glycine, 1.5 wt% mannitol, and 0.1 wt% potassium sorbate, demonstrated moderate thermal conductivity of 0.6017 W/(m·K) and a suitable melting temperature of −5.78 °C as well as an enthalpy of fusion of 292.64 J/g. After 100 thermal cycles, the melting temperature, enthalpy of fusion, and thermal conductivity were −5.91 °C, 290.66 J/g, and 0.5886 W/(m·K), which decreased only by 2.24%, 0.68%, and 2.18%, respectively. Furthermore, the cold storage performances of 6-PCM5 and ice were compared and evaluated by loading strawberries. Compared with ice storage, the pre-cooling time and average temperature of strawberries in the cold storage box with 6-PCM5 was reduced by 68.4% and 2.55 °C, and the effective cold storage time was lasted for 22.83 h at a temperature range of 0.45 °C to 3 °C, which effectively improved the cold storage quality of strawberries. Overall, the developed 6-PCM5 cold storage material possesses good static and cyclic stability, providing an effective solution for pre-cooling and short-distance cold chain transportation of perishable food.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2023
·
IF 3.8
Jingxia Wang,Xiaohan Shan,Qiang Xue,Yao Liu,Zeyu Liu,Lin He,Xiaolei Wang,Changchun Zhu
In this work, we firstly developed a laser-induced graphene (LIG) electrode modified by gold nanoparticles (AuNPs) and glycine-modified nanocarbon (Gly-C), and used it to detect nitrite by linear scanning voltammetry . The physical and electrochemical characteristics of the electrodes were characterized using scanning electron microscopy, energy dispersive spectroscopy analysis, fourier transform infrared spectroscopy , and contact angle analysis. Our findings demonstrated that the electrocatalytic oxidation of nitrite can be enhanced by the successful loading of AuNPs, and Gly-C enhances electrode hydrophilicity and promotes nitrite adsorption capacity of the electrode surface. Under optimal conditions, the linear of the sensor is 7 ∼ 700 μg/L and 700 ∼ 1050 μg/L, and the detection limit is 0.84 μg/L. The anti-interference, repeatability, and stability of this sensor are outstanding. Recovery rates of the sensor in the actual groundwater and tap water varied from 94 to 123 %, and the relative standard deviation was less than 6.06 %. Our findings offer a potentially effective method for on-site monitoring of nitrite in aquatic environments.
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