ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 27
·6813-6821
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IF 4.157
Ma Liang,Zhu Yindi,Chen Xueming,Fang Raohao,Chen Yuru,Xu Xia,Huang Guozheng,Liu Zi,Liu Xiang
Nano-magnetite with superparamagnetism could be coated by some organic compounds or by nano Au or Pt via surface modifications with multi-step reactions for the applications of isolating histidine-tagged (His-tagged) proteins. Introducing active sites of binding histidine onto the surface of nano-magnetite was the ultimate task. However, multi-step treatments might result in departure of the coatings from the surface of the nano-magnetite, which led to loss of active sites. In this work, we reported a convenient and efficient way of treating nano-magnetites and applied them in isolating His-tagged proteins. Carboxylates were introduced on the surface of home-made nano-magnetite directly via ultrasonic mixing with sodium bitartrate rather than complicated surface modifications, which was proved by thermogravimetric analyses. Ni 2+ was, therefore, caught by the carboxylates of the coating via the coordinate interaction, demonstrated by X-ray photoelectron spectra. The coated magnetic nanoparticles with the bonded Ni 2+ were successfully employed to selectively bind and separate recombinant His-tagged proteins directly from the mixture of Escherichia coli cell lysate, and showed wonderful affinity for His-tagged proteins with the saturated adsorption amount being 556 mg g −1 . Additionally, such functionalized nano-magnetite manifested the excellent recyclability in isolating His-tagged proteins. Graphical abstract
研究领域:
纳米技术
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生物化学
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蛋白质工程
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分子生物学
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Shuting Chen,Weijia Wang,Shaohua Xu,Caili Fu,Shuyi Ji,Fang Luo,Cuiying Lin,Bin Qiu,Zhenyu Lin
In this work, an auto-identify sensor was constructed for rapid and high-precision detection of L-histidine. The proposed strategy is based on the auto-identify algorithm and the aggregation of alkynyl and azide functionalized gold nanoparticles induced by the Cu + catalyzed azides and alkynes cycloaddition (CuAAC) reaction. Specially, the color of scattering light spots for the aggregated gold nanoparticle (AuNPs) caused by CuAAC reaction was quite different from that of the monomers . However, L-histidine can bind to Cu 2+ and inhibits the production of Cu + , hence preventing the aggregation of AuNPs. Therefore, there is a distinct change of color as the addition of L-histidine under dark-field microscopy. Then, L-histidine can be quantitatively detected by combining the color change with the Meanshift algorithm accurately and automatically. Such proposed method has been successfully applied for the detection of L-histidine in serum sample with satisfying result.
研究领域:
分析化学
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纳米技术
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生物化学
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DOI
PubMed
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 10
·e202204406
·
IF 2.1
Chibin Pu,Zhongxi Huang,Lihua Huang,Qian Shen,Changmin Yu
Graphical We firstly reported the direct detection and quantification of His-tagged protein based on intramolecular rigidification induced emission in dilute solution, achieved the real-time and fast fluorescent “On” response of His-tagged proteins in protein solution. Our probe could be used in fluorescent labeling of proteins with simple procedure, high quality and low background interference. Histidine tag (His-tag) is a representative epitope tag, which has been widely used in the purification or immobilization of recombinant proteins. In this study, a novel probe CTPE-IDA-Ni 2+ based on intramolecular rigidification induced emission for fluorescence detecting His-tagged proteins is successfully developed and experimentally demonstrated. This detecting system is based on TPE derivative incorporated IDA-Ni 2+ moiety for real-time detection of His-tagged proteins. In the presence of His-tagged proteins, the IDA-Ni 2+ -His-tag complex was formed, which restricted the rotation of two phenyl rings linked with TPE and subsequently resulting in the fluorescence turn on. Results have shown that our probe could directly detect His-tagged proteins with good selectivity and fast response within 10 min. In addition, we have proved that this system could be used in fluorescent labeling of His-tagged protein instead of traditional Western blot assay with simple procedure, high quality and low background interference, as well as without aggregation-caused quenching (ACQ) problems like some organic dyes.
研究领域:
分析化学
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生物化学
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分子生物学
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蛋白质工程
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DOI
高影响因子
mSphere
·
2025
·Vol. 10
·期 6
·
IF 3.1
Jieying Li,Yuxiao Liu,Jingsi Jiang,Fang Chen,Nan Zhang,Xun Kang,Lin Liu,Yingjuan Wang,Qianfeng Xia,Chuanlong Zhu,Dai Kuang
Klebsiella pneumoniae is a globally recognized microbial pathogen with significant clinical impact. The bacterium harbors the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems, which provide adaptive immunity against invading foreign nucleic acids. Recent studies suggest that certain CRISPR-Cas systems can regulate endogenous genes, influencing bacterial virulence. However, their role in regulating pathogenicity in K. pneumoniae remains poorly understood. This study investigates the regulatory role of the type I-E* CRISPR-Cas system in a hypervirulent K. pneumoniae strain, focusing on its impact on histidine metabolism and pathogenicity. Transcriptome analyses identified differentially expressed genes (DEGs) between the casABECD-deletion and wild-type strains, including significant upregulation of the histidine utilization (Hut) operon and downregulation of biofilm-related genes. These molecular changes resulted in enhanced histidine metabolic activity, reduced biofilm formation, attenuated virulence in A549 lung epithelial cells, and improved survival of Galleria mellonella, as validated through phenotypic and virulence assays. Our bioinformatic analysis indicated that the CRISPR-Cas system in K. pneumoniae targets the hutT sequence, which is part of the Hut operon. Furthermore, the overexpression of hutT mitigated CRISPR-Cas-mediated repression of the Hut operon, as observed in virulence assays, while simultaneous deletion of hutH and casABECD restored the reduced virulence in the ΔcasABECD strain. Additionally, deletion of casABECD significantly enhances the growth of the strain in medium with histidine as the sole carbon source, highlighting the intricate regulatory role of the CRISPR-Cas system in metabolic adaptation. Collectively, these findings uncover a novel role for the CRISPR-Cas system in regulating metabolic pathways and virulence in hypervirulent K. pneumoniae.
研究领域:
微生物学
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分子遗传学
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传染病学
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细菌致病机制
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CRISPR生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 6
·1865-1872
·
IF 4.831
Huang Pengcheng,Li Jianfang,Song Juan,Gao Nan,Wu Fangying
The authors demonstrate a single-step histidine assay that is based on the use of silver nanoparticles modified with sulfanilic acid (SAA-AgNPs). The presence of histidine leads to a visually detectable gradual color change from bright yellow via orange to purple. The effect is assumed to be mainly due to strong π-π stacking, electrostatic interaction, and hydrogen bonding between SAA and histidine. The assay has a 52.7 nM detection limit and works in the 0 to 3.5 μM concentration range. It is selective over other compounds when using appropriate masking agents. The method has been successfully applied to the colorimetric determination of histidine in (spiked) serum samples. Recoveries ranged between 97 % and 107 %, and relative standard deviations are <0.92 % (for n = 3). The method was also applied to detect polyhistidine-tagged cysteine (His-His-His-His-His-His-Cys) which can be quantified in concentrations down to 5.57 nM. This finding links the method to His-tag technology.
A single-step colorimetric assay for histidine is developed using sulfanilic acid modified silver nanoparticles (SAA-AgNPs) via π stacking, electrostatic interaction, and hydrogen bonding resulting in the aggregation of the AgNPs and a color change from yellow to orange.
研究领域:
分析化学
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生物化学
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医学诊断
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2026
·
IF 9
Ruohan Liang,Dongsheng Zhao,Fangming Jin,Jun Xu,Guicai Liu
The limited mono−/divalent anion selectivity and inadequate antifouling properties of polyamide (PA) nanofiltration (NF) membranes restrict their use in water treatment. Herein, L-histidine (L-His)-modified NF membranes were prepared through interfacial polymerization on a polyethersulfone porous substrate. The aqueous-phase comonomers were piperazine and L-His, which contain both amino and carboxyl groups, while the organic-phase monomer was trimesoyl chloride. Compared with the NF0 membrane without addition of L-His, all of the L-His-modified membrane was characterized by a higher surface roughness, a thinner PA layer, greater hydrophilicity, a more negative surface charge and a larger mean pore size. Among them, the optimal NF0.5 membrane with the addition of 0.5% ( w / v ) L-His demonstrated a pure water permeability of 17.9 L/(m 2 ·h·bar) and a 2.7-fold higher NaCl/Na 2 SO 4 selectivity (up to 102.0). In particular, the NaCl/Na 2 SO 4 selectivity improved with higher operating pressure, but decreased with increasing single-salt concentration or NaCl/Na 2 SO 4 mass ratio in the feed solution. In addition, the NF0.5 membrane demonstrated superior resistance to protein fouling and outstanding long-term operational stability throughout a continuous 7-day filtration process. This study could provide valuable insights into the development of NF membranes with high mono−/divalent anion selectivity and excellent antifouling properties.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Xiao-Han Yuan,Wen-Sheng Liu,Jian-Hua Zhang
Formaldehyde (FA), the simplest and widely-applied aldehyde, can pose serious health issues when present at elevated concentrations. Herein, this study proposes a self-calibrating ratiometric sensing strategy for the sensitive detection of FA, which is based on the dual-functional ligands and multi-metal synergistic effects of L-Histidine (L-His)-functionalized Lanthanide metal-organic gels (MOGs). In this strategy, L-His and 1,3,5-benzenetricarboxylic acid (BTC) served as functional ligands coordinating with Zr 4+ , Eu 3+ , and Tb 3+ metal centers, forming three MOGs with superior photoluminescence properties: ZrEuTb-His-MOG, ZrEu-His-MOG, and ZrTb-His-MOG. Notably, ZrEuTb-His-MOG exhibits a prominent multi-metal synergistic effect: a stable self-calibration signal is generated through Zr 4+ -(L-His) ligand-to-metal charge transfer (LMCT), while the antenna effect of BTC to Tb 3+ facilitates a FA-responsive specific fluorescent signal. The synergistic interaction between these two mechanisms establishes a highly reliable self-calibrating detection system. This sensor achieves high sensitivity with a detection limit of 8.4 nM, a broad linear range of 0.001 μM–60 μM, and excellent selectivity against common interfering species. Moreover, ZrEuTb-His-MOG exhibits outstanding regenerability and long-term signal stability, qualifying it as an efficient and recyclable fluorescent probe for FA detection. Additionally, we developed a portable fluorescent test strip based on ZrEuTb-His-MOG, enabling “on-off” visual detection of both aqueous and gaseous FA with a fast response. This work provides a novel and reliable strategy for on-site FA monitoring in environmental monitoring, food safety, and public health protection.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Miao Yuan,Saidan Wang,Hongxia Du,Yating Sui,Xiangjing Zhang,Song Zhao,Qiushi Li,Shaojie Liu,Xuteng Xing,Erjun Tang
Two-dimensional Ti 3 C 2 T x MXene, with a large specific surface area, high chemical stability and excellent barrier properties, has become a promising of anti-corrosion filler in organic coatings. However, the poor dispersion and compatibility in organic coatings restrict its efficient application. To this end, L-histidine (L-His) corrosion inhibitor was grafted on the 3-aminopropyl triethoxysilane (APTES) functionalized Ti 3 C 2 T x MXene surface, under the electrostatic attraction between the carboxylate and protonated aminopropyl groups. The obtained APTES-Ti 3 C 2 T x MXene@L-His cannot only accelerate the dispersion of Ti 3 C 2 T x MXene filler, but also greatly promote the anti-corrosion property and service lifetime of coatings through combining the physical shielding effect of MXene laminate structure with active anti-corrosion function of L-His corrosion inhibitor. Results show that amino-functionalized Ti 3 C 2 T x MXene can maintain homogeneous in epoxy resin for 300 h, enhancing its dispersion in epoxy coating. Moreover, APTES-Ti 3 C 2 T x MXene@L-His composite can achieve the continuous release of L-His for 3000 min, and additional acid-base dual response property, the releasing rate of L-His is significantly accelerated in acidic or alkaline solutions. The total loading amount of L-His in APTES-Ti 3 C 2 T x MXene@L-His composite is about 3 %. Furthermore, the practical composite coating containing APTES-Ti 3 C 2 T x MXene@L-His also exhibits the excellent anti-corrosion and certain self-healing properties, such as its |Z 0.01 Hz| value remains above 10 10 Ω·cm 2 during the 40 d immersing in 3.5 NaCl solution, which is 3 orders of magnitude higher than that of pure epoxy coating. This work extends the great significance for Ti 3 C 2 T x MXene based composite coatings application of in the field of anti-corrosion.
研究领域:
材料科学
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腐蚀与防护
·
有机涂层改性
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Sunlong Li,Siyu Hu,Yifeng Shi,Yeheng Huang,Yuli Chen,Yan Chen,Fengyu Zhang,Zhan Gao,Shuqing Jin,Qiwei Zhou,Shu Yang,Haibo Liang,Lianggao Yu,Shuhao Zhang,Chongan Huang,Chenglong Xie,Zaher Meftah,Aimin Wu,Xiaolei Zhang,Xiangyang Wang
Ferroptosis is a major contributor to intervertebral disc degeneration (IVDD) and represents a promising therapeutic target; however, effective medications that specifically target ferroptosis are still lacking. Consequently, strategies aimed at suppressing the expression of ferroptosis-related genes, such as small interfering RNA (siRNA) targeting activating transcription factor 3 (ATF3), appear to be a feasible therapeutic approach for IVDD. Zeolitic imidazolate framework-8 (ZIF-8) exhibits exceptionally high binding affinity for nucleic acids, including siRNA. Nevertheless, its practical application is hindered by challenges such as limited siRNA encapsulation and potential toxicity arising from Zn 2 ⁺ ion release during degradation. In the current study, a novel siRNA delivery system is designed with low toxicity, high encapsulation efficiency, and sustained release by hybridizing classical ZIF-8 with histidine (H-ZIF-8) through defect engineering. In vitro functional studies demonstrated that H-ZIF-8 significantly enhances the delivery efficiency of siATF3 in nucleus pulposus cells (NPCs), and effectively suppresses ferroptosis and extracellular matrix (ECM) degradation. Furthermore, the incorporation of histidine into ZIF-8 may improve its biocompatibility by reducing the proportion of Zn 2 ⁺ ions present. In vivo, siATF3@H-ZIF-8 significantly inhibited ferroptosis and alleviated intervertebral disc degeneration (IVDD) in a rat model through sustaining ATF3 knockdown. This research suggests that histidine-modified ZIF-8 may serve as a novel system for siRNA delivery and an effective gene therapy method for diseases, including IVDD.
研究领域:
生物医学工程
·
基因治疗
·
材料科学
·
骨科
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DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 15
·2386-2399
·
IF 2.7
Shili Qin,Fanshu Meng,Fenglong Jin,Xidi Xu,Ming Zhao,Hongtao Chu,Lidi Gao,Shuren Liu
A novel fluorescence sensor based on a porphyrinic zirconium-based metal–organic framework, L-cysteine-modified PCN-222 (L-Cys/PCN-222), was developed to selectively recognize histidine enantiomers and sensitively detect Hg2+. The dual-functional sensor was successfully prepared via the solvent-assisted ligand incorporation method and characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), 1H nuclear magnetic resonance (1H NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD), X-ray photoelectron spectroscopy (XPS), and nitrogen adsorption–desorption analyses. L-Cys/PCN-222 not only showed a higher quenching response for L-histidine than that for D-histidine with a fast fluorescent response rate of <40 s but also exhibited low detection limits for L- and D-histidine (2.48 μmol L−1 and 3.85 μmol L−1, respectively). Moreover, L-Cys/PCN-222 was employed as a fluorescent and visual sensor for the highly sensitive detection of Hg2+ in the linear range of 10–500 μmol L−1, and the detection limit was calculated to be 2.79 μmol L−1 in surface water. The specific and selective recognition of chiral compounds and metal ions by our probe make it suitable for real field applications.
研究领域:
分析化学
·
材料科学
·
生物化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Ruidi Shen,Li Zou,Shixin Wu,Tingting Li,Jing Wang,Jianmin Liu,Liansheng Ling
A novel label-free fluorescent sensor for histidine was developed based upon Cu 2+ -specific DNAzyme, hybridization chain reaction(HCR) and triplex DNA . Cu 2+ can bind to the histidine, in the presence of histidine, leading to the inhibition of the cleavage of substrate strand of Cu 2+ -dependent DNAzyme, then the intact substrate strand trigger the HCR between H1 and H2. The HCR product can be recognized by triplex-forming oligonucleotide (TFO) through triplex formation and reported by the fluorescence of berberine , the fluorescence intensity of the sensing system was proportional to the concentration of histidine during the range of 5.7–455 nmol L −1 , with a detection limit of 2.0 nmol L −1 .
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Changjun Li,Naiyun Gao,Wenhai Chu,Tom Bond,Xingya Wei
Trihalomethane (THMs) and haloacetonitriles (HANs) are typical and frequently detected carbonaceous (C–) and nitrogenous (N–) disinfection by-products (DBPs) in water treatment , and amine acids (AAs) are important precursors of DBPs. However, previous researches mainly focused on free AAs and few investigations evaluated the DBPs formation potential of combined AAs, especially combined histidine . The formation of THMs and HANs from free and combined histidine and glycine during chlorination was firstly examined and compared in this study with an emphasis on their differences. The impacts of chlorine dose, pH, ammonia nitrogen and bromide were evaluated and mechanistic formation pathways were proposed. In comparison with free AAs, combined AAs generated more chloroform (CF, 125%–671%) but less dichloroacetonitrile (DCAN, 4.66%–87.5%) at all chlorine doses. The effect of solution pH on combined AAs was slighter than that on free AAs. During chloramination, both free AAs and combined AAs generated much smaller amounts of DBPs, especially DCAN (<9.01%), compared with chlorination. At any bromide dose, there was more bromine merging into THMs than DHANs, especially for combined AAs. At the beginning of chlorination, the combined AAs decomposed in three different ways: hydrolysis, decarboxylation and scission , leading to differing amounts of CF and DCAN compared with the free AAs.
研究领域:
环境科学
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水处理工程
·
化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）