ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Sipeng Tian,Chao Peng,Huanhuan Xing,Yuan Xue,Jing Li,Erkang Wang
Modulating the photon emission of the luminophore for boosting chemiluminescence (CL) response is very crucial for the construction of highly sensitive sensors via the introduction of functionalized materials. Herein, the integration of the emitter and coreactant accelerator into one entity is realized by simply assembling cucurbit[7]uril (CB[7]) on the surface of gold nanoparticles (AuNPs) through simple assembly via a Au–O bond. The loaded CB[7] on the AuNPs improves their catalytic capacity for the generation of hydroxyl radicals(•OH). Moreover, the host–guest recognition interaction between luminol and CB[7] enables the capture of luminol on AuNPs efficiently. Also, the intramolecular electron-transfer reaction between the luminol and •OH enables the CL response more effectively in the entity, which greatly boosts photon emission ca 100 folds compared with the individual luminol/H2O2. The host–guest recognition between luminol and CB[7] is revealed by Fourier transform infrared spectroscopy, electrochemical, and thermogravimetric characterization. Moreover, the proposed CL system is successfully used for the sensitive and selective determination of dopamine (DA) based on a synergistic quenching mechanism including the competition quenching and radical-scavenging effect from DA. The present amplified strategy by integrating recognized and amplified elements within one entity simplifies the sensing process and holds great potential for sensitive analysis based on the self-enhanced strategies.
研究领域:
分析化学
·
材料科学
·
纳米技术
·
传感器
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yao Peng,Linying Yu,Mengting Sheng,Qian Wang,Zhiying Jin,Jianshe Huang,Xiurong Yang
Two-dimensional (2D) iron/cobalt metal–organic framework nanosheets (Fe/Co-MOF NSs) were synthesized via the cooperative self-assembly reaction of Fe3+/Co2+ and terephthalic acid at room temperature. The as-prepared 2D Fe/Co-MOF NSs display superior performance in catalysis of the chemiluminescence (CL) reaction between luminol and H2O2. The CL spectrum, UV–vis absorption spectroscopy, radical scavenger experiments, and electron spin resonance (ESR) spectroscopy are utilized to research the possible CL mechanism of the luminol-H2O2–Fe/Co-MOF NSs system. All results indicate that Fe/Co-MOF NSs present outstanding peroxidase-like activity and could catalyze H2O2 to produce 1O2, O2·–, and ·OH, which could react rapidly with the luminol anion radical and result in strong CL. With the highly efficient CL of the luminol-H2O2–Fe/Co-MOF NSs system, a sensitive sensor for the detection of dopamine (DA) is developed based on the inhibitory effect of DA on the CL intensity. Good linearity over the range of 50–800 nM is achieved with a limit of detection of 20.88 nM (S/N = 3). This research demonstrates that 2D Fe/Co-MOF NSs is a highly effective catalyst for luminol CL reaction and has great application potential in the CL field.
研究领域:
材料科学
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分析化学
·
生物医学工程
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PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 23
·4363
·
IF 5.2
Zhiping Yu,Chengqiang Li,Jiashuai Sun,Xia Sun,Guodong Hu
The accumulation of pesticide residues poses a significant threat to the health of people and the surrounding ecological systems. However, traditional methods are not only costly but require expertise in analysis. An electrochemiluminescence (ECL) aptasensor was developed using chitosan and molybdenum disulfide (CTS-MoS2), along with acetylene black (AB@CTS) for the rapid detection of malathion residues. Due to the weak interaction force, simple composite may lead to uneven dispersion; MoS2and AB were dissolved in CTS solution, respectively, and utilized the biocompatibility of CTS to interact with each other on the electrode. The MoS2nanosheets provided a large specific surface area, enhancing the utilization rate of catalytic materials, while AB exhibited excellent conductivity. Additionally, the dendritic polylysine (PLL) contained numerous amino groups to load abundant luminol to catalyze hydrogen peroxide (H2O2) and generate reactive oxygen species (ROS). The proposed ECL aptasensor obtained a low detection limit of 2.75 × 10−3ng/mL (S/N = 3) with a good detection range from 1.0 × 10−2ng/mL to 1.0 × 103ng/mL, demonstrating excellent specificity, repeatability, and stability. Moreover, the ECL aptasensor was successfully applied for detecting malathion pesticide residues in authentic samples with recovery rates ranging from 94.21% to 99.63% (RSD < 2.52%). This work offers valuable insights for advancing ECL sensor technology in future applications.Keywords:electrochemiluminescence;aptamer;CTS-MoS2;AB@CTS;PLL-luminol;nano gold
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiayi Wu,Lujie Wang,Hongxin Wang,Xinhui Gu,Yucheng Zhou,Fengna Xi
Sensitive detection of alpha-fetoprotein (AFP) is crucial for early liver cancer screening. In this work, an immunosensor is constructed by combining the immunorecognition interface and enhanced electrochemiluminescence (ECL) of luminol under neutral condition utilizing cobalt oxide (Co 3 O 4 ) nanozymes confined in nanochannels, which realizes highly sensitive detection of AFP. Indium tin oxide (ITO) conductive glass is used as the supporting electrode, and silica nanochannel film (SNF) is simply grown on the ITO surface using the Stöber method. Co 3 O 4 nanozymes are in situ grown within the SNF nanochannels by a one-step electrodeposition method. The rigid 2D nanochannel array of SNF effectively confines the Co 3 O 4 nanozyme, significantly enhancing its stability. Epoxy modification on the external surface of SNF enables covalent attachment of AFP antibodies, leading to recognition interface. Confined Co 3 O 4 exhibits peroxidase-like activity, catalyzing the generation of reactive oxygen species (ROS) from hydrogen peroxide (H 2 O 2 ), enhancing luminol oxidation and boosting the ECL signal under neutral conditions. Upon AFP binding, antibodies capture AFP to form immunocomplexes, causing reduced diffusion of luminol and H 2 O 2 to the electrode surface, decreasing the ECL signal. The constructed immunosensor exhibits a linear range for AFP detection from 100 fg/mL to 10 ng/mL, with a limit of detection (LOD) of 18.5 fg/mL. AFP detection in serum samples from liver cancer patients or healthy individuals is successfully achieved.
研究领域:
生物医学工程
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分析化学
·
癌症诊断
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Yang Zhou,Jianxiu Du,Zhen Wang
Fluorescein and its derivatives, including 4′,5′–dibromofluorescein, 2′,7′–dichlorofluorescein and 2′,4′,5′,7′–tetrabromofluorescein can be employed as efficient coreactants for luminol reaction. Under an alkaline condition they reacted with luminol to produce measureable chemiluminescence (CL) in the absence of any extra–oxidant. Cobalt ion (Co 2+ ) significantly enhanced the CL emission of the reaction. The CL emission was strongly dependent on the concentration of carbonate buffer used; higher concentration of carbonate buffer provided more intense CL emission. The system had two emission peaks locating at 425 nm and 535 nm, respectively. With 4′,5′–dibromofluorescein as the coreactant for luminol reaction, a new CL method was developed for the detection of Co 2+ . The procedure allowed the linear detection of Co 2+ in the range of 5–1000 nmol/L. The limit of detection (LOD) and limit of quantitation (LOQ) were 1.8 nmol/L and 5.0 nmol/L, respectively. The intra–day precision (n = 11) and inter–day precision (n = 3) was 3.2% and 4.7% for replicated measurements of 0.2 μmol/L Co 2+ solution. The method was successfully applied to the determination of Co 2+ in blue silica gel samples.
研究领域:
分析化学
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环境科学
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材料科学
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PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 42
·23720-23726
·
IF 2.936
Huihui Xu,Qiyong Cai,Qiuyu Nie,Zhun Qiao,Song Liu,Zhaohui Li
Ascorbic acid (AA) as an essential vitamin in the human body participates in various physiological reactions and plays a key role in many biochemical processes. Therefore, it is of vital importance to monitor and quantify AA in commercial tablets, beverages and food. In this work, a rapid and ultrasensitive chemiluminescence (CL) system for the detection of AA was developed, in which ultrathin cobalt oxyhydroxide (CoOOH) nanosheets were applied in the conventional luminol–H2O2 CL system. The results showed that ultrathin CoOOH nanosheets as a catalyzer remarkably improved the CL intensity of the CoOOH–luminol–H2O2 system, up to about 1400-fold. Under the optimized conditions, the CL inhibition efficiencies increase linearly with the concentrations of AA in the range of 1–500 pmol L−1, and the limit of detection was 39 fmol L−1. Moreover, the proposed CL system was successfully applied in the determination of AA in medicinal tablets with satisfactory results.
研究领域:
分析化学
·
生物医学
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PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2022
·
IF 5.141
Hong Li,Yang Zhou,Jianxiu Du
Ascorbic acid can serve as an efficient coreactant for luminol chemiluminescence (CL) reaction. An observable CL emission is produced when mixing ascorbic acid with luminol in an alkaline condition. The CL signal is further dramatically enhanced by cobalt(II) ions. This CL system was exploited as a new method for direct determination of ascorbic acid with a flow injection mode. A good linear response is acquired between the CL signal and ascorbic acid concentration in 5.0–1000.0 ng/mL range with a detection limit of 1.3 ng/mL. The relative standard deviations are 2.3% for intra–day (n = 11) and 3.7% for inter–day (n = 3) by parallel determination of 0.5 μg/mL ascorbic acid solution. The method has good selectivity for ascorbic acid since no obvious responses are observed for a variety of inorganic ions and biological substances. The practicability of the method has been demonstrated by the analysis of ascorbic acid in some soft drinks.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jun Chen,Xuanxu Nan,Li Yang,Yue Cui
Cardiac troponin I (cTnI) is a highly specific biomarker of cardiomyocyte injury, released during cell disintegration and necrosis, and is the gold standard for diagnosing acute myocardial infarction (AMI). At the onset of AMI, cTnI appears in very low concentrations (pg/mL level), necessitating the development of highly sensitive and rapid detection sensors. In this study, an electrochemiluminescence lateral flow immunosensor (ECL-LFI) was designed using luminol-labeled silver nanoparticles (luminol@AgNPs) for the sensitive and quantitative detection of cTnI. A screen-printed electrode (SPE) was integrated beneath the nitrocellulose (NC) membrane with plastic plates of the same thickness applied on both sides of the SPE to ensure a smooth flow surface. Upon addition of cTnI and the luminol-H 2 O 2 system, sandwich immune complexes formed by antibody-functionalized luminol@AgNPs on the strips generated electrochemiluminescent (ECL) signals. The ECL-LFI exhibited a broad linear detection range from 5 pg/mL to 100 ng/mL, with a detection limit as low as 1.6 pg/mL. Additionally, the results show excellent correlation with clinical tests, demonstrating that the ECL-LFI provides a promising point-of-care tool for the early diagnosis of AMI and other diseases.
研究领域:
生物医学工程
·
临床诊断学
·
纳米技术
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DOI
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 11
·1-10
·
IF 5.3
Chen Zixuan,Ma Shuhui,Wang Chujun,Liu Jie,Yang Xiaoyan
Monitoring of the amyloid-beta (A β ) peptide is crucial for the diagnosis and treatment of Alzheimer’s disease. Here, a label-free, signal-on ratiometric electrochemiluminescence (ECL) aptasensor was fabricated for the detection of A β peptide. Specifically, graphite-like carbon nitride (g-C 3 N 4 ) nanosheets and luminol served as the cathodic and anodic ECL emitters, respectively. K 2 S 2 O 8 and H 2 O 2 were used as the coreactants for the two emitters. Luminol was first coated on the indium tin oxide (ITO) electrode and used as the internal standard in the ratiometric biosensor. Polyaniline was then electrodeposited on the surface of the luminol-modified electrode to improve the biocompatibility and conductivity of the sensing interfaces. Gold nanoparticles were subsequently modified on the surface of polyaniline to immobilize a DNA aptamer. g-C 3 N 4 -heme incubated with different concentrations of A β solution to form g-C 3 N 4 -heme-A β complex, in which A β could specifically bind to the DNA aptamer and thus introduce g-C 3 N 4 into the system. As a result, it was observed that the ratio value of ECL intensity ( I 1 / I 2 ) significantly increased with the increase of A β concentration. Under the optimized conditions, the biosensor has a good linear response in the range 10 fM to 10 nM with a detection limit of 4.2 fM. Consequently, the ratiometric ECL sensor, characterized by high sensitivity and favorable stability, offers a reliable method for the determination of A β . Graphical
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高影响因子
TALANTA
·
2018
·
IF 4.244
Kaile Tian,Dejian Li,Tingting Tang,Fei Nie,Yang Zhou,Jianxiu Du,Jianbin Zheng
Luminol-nitrogen doped graphene quantum dot (luminol-NGQDs) nanocomposite was synthesized and a novel electrochemiluminescence resonance energy transfer (ECL-RET) process occurred between luminol as the donor and NGQDs as the acceptor in the composite. This ECL-RET effect helped luminol-NGQDs composite produced an anodic ECL signal without coreactants. The ECL-RET mechanism was also studied based on the fluorescence spectra, the ultraviolet–visible absorption spectra and the electrochemiluminescence (ECL) spectra. Based on the significant sensitization effect of hydrogen peroxide on luminol-NGQDs ECL signal, an ECL method for the sensitive determination of hydrogen peroxide was established and then applied to the detection of hydrogen peroxide in water samples.
研究领域:
分析化学
·
纳米材料
·
电分析化学
·
生物传感器
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Hao Xue,Tianyi Ma,Hong Zheng,Luoyilan Zhang,Ke Cheng
Biomass-derived fluorine and nitrogen co-doped carbon dots (F, N-CDs) were synthesized via a one-step hydrothermal method using Rhododendron simsii flowers as the precursor. The prepared F, N-CDs exhibited favorable optical properties, good water solubility, and a pronounced enhancement effect on the chemiluminescence (CL) of the Luminol-K 2 S 2 O 8 -NaOH system. The optical characteristics were systematically investigated by UV–Vis absorption spectroscopy, fluorescence spectroscopy, and fluorescence lifetime analysis. The possible CL enhancement mechanism was further investigated through reactive oxygen species scavenging experiments and singlet oxygen probe experiments. On this basis, an enhanced flow-injection chemiluminescence (FI-CL) method was developed for the determination of levetiracetam (LEV). Under the optimized conditions, the relative CL intensity exhibited a satisfactory linear relationship with LEV concentration over the range of 0.10–10.00 mM, with a limit of detection (LOD) of 2.02 × 10 −3 mM and a limit of quantification (LOQ) of 6.71 × 10 −3 mM. The proposed method was successfully applied to the commercial LEV pharmaceutical formulations, giving recoveries of 94.8–103.1% and relative standard deviations (RSDs) below 4.68%. These results demonstrate the potential of biomass-derived carbon dots for spectroscopic and characterization and chemiluminescence-based analytical applications.
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高影响因子
ANALYTICAL SCIENCES
·
2021
·Vol. 37
·期 11
·1525-1531
·
IF 2.081
Zhifang DING,Yue LI,Ying BAO,Kexin HAN,Iparguli TUREPU,Guixin LI
A signal-on supersandwich type of electrochemiluminescence (ECL) DNA biosensor was developed based on the poly(aniline-luminol) nanowires (PALNWs) modified electrode and enhancement of ferrocene (Fc) on ECL of luminol. Aminated capture DNA was covalently linked to the PALNWs on the electrode surface by the crosslinking of glutaraldehyde. In presence of target DNA, its 3′ terminus hybridizes with the capture probe and the 5′ terminus hybridizes with ferrocene labeled DNA (Fc-DNA) to form a long DNA concatamer supersandwich structure. The ECL intensity of the prepared biosensor was clearly improved by increasing the concentration of target DNA due to the enhancement of ferrocene on luminol ECL. The difference of the ECL intensity in the absence and presence of target DNA was used to monitor the hybridization event. The difference of ECL linearly increased with the logarithm of target DNA concentration in the range from 1.0 × 10−16 – 1.0 × 10−8 mol L−1 with a detection limit of 5.8 × 10−17 mol L−1. The sensor had high sensitivity and wide linear relationship for the detection of target DNA.
研究领域:
分析化学
·
生物传感技术
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高影响因子
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