Food Science and Human Wellness
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2024
·
IF 5.6
Jihua Mao,Yanhong Shao,Hui Wang,Jun Liu,Zongcai Tu
Gut microbiota plays an important role in food allergy. The immunoglobulin G (IgG)/immunoglobulin E (IgE) binding capacity and human gut microbiota changes of digestion products derived from glycated ovalbumin (OVA) were investigated. Gastrointestinal digestion effectively destroyed the primary structure of glycated OVA, resulting in a significantly higher digestibility than gastric digestion, and more abundant peptides < 3 kDa. Moreover, gastric and gastrointestinal digestion products have different fluorescence quenching and red shift of fluorescence peaks, and possess different conformational structures. These changes resulted in a decrease in 28.7 % of the IgE binding capacity of gastrointestinal digestion products beyond that of pepsin. Moreover, gastrointestinal digestion products of glycated OVA increased significantly the proportion of Subdoligranulum , Collinsella , and Bifidobacterium . Therefore, gastrointestinal digestion products of glycated OVA altered human intestinal microbiota, reducing the risk of potential allergy.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Chaoran Yang,Wei Yu,Boyan Gao,Yuge Niu,Liangli Yu
To improve the resistance of Monascus pigments (MPs) to adverse environmental factors, a nanoparticle carrier was constructed using ovalbumin, rutin and pectin modified by octenyl succinic anhydride. According to the determination of particle size, ζ-potential and microstructure, the composite nanoparticles exhibited the "core-shell" structure. Specifically, the ovalbumin core was encapsulated by the modified pectin adsorbed with rutin. Spectroscopic analysis demonstrated that electrostatic interactions, hydrogen bonding and hydrophobic interactions between wall materials and MPs were the main driving forces to form composite nanoparticles. The stabilities of light, thermal and storage of MPs were significantly enhanced after encapsulation due to the physical barrier effect of the wall materials against external disturbances and the multiple functions of rutin, including free radical scavenging, absorption of partial UV and scatter light, and possible co-pigmentation effects with MPs. Moreover, the composite nanoparticles exhibited higher inhibition activity against pancreatic lipase and antioxidant activity compared with those of free MPs. The results provided an ideal way to develop new MPs products, which had long-lasting and stable performance during food processing and storage.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhi-Wei Liu,Pan-Pan Tang,Yi-Xuan Zhang,Jun-Hu Cheng,Rana Muhammad Aadil,Xiu-Bin Liu
The impact of Dielectric-Barrier Discharge (DBD) plasma treatment on the prevention of heat-induced aggregation of Ovalbumin (OVA) and improvement in emulsification properties was investigated. Results highlighted the effective inhibition of thermal aggregation of OVA following exposure to plasma. Structural analysis revealed that the plasma-induced oxidation of sulfhydryl and intermolecular disulfide bonds played a pivotal role in inhibiting the thermal aggregation, considered by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), multiplies spectroscopy, and analysis of dynamic exchange of sulfhydryl-disulfide bonds. Meanwhile, the oxidation of exposed hydrophobic sites due to plasma treatment resulted in the transformation of the OVA molecule's surface from hydrophobic to hydrophilic, contributing significantly to the aggregation inhibition. Additionally, compared to an untreated sample of OVA, almost one-fold increase in emulsifying ability (EAI) and 1.5-fold in emulsifying stability (ESI) was observed after 4 min of plasma treatment. These findings demonstrated that plasma treatment not only enhanced the thermal stability of OVA, but also improved its emulsification properties.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
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IF 4
Songhong Zhang,Xinyu Gao,Mingyan Zhang,Yuye Wang,Weilong Cheng,Wenjing Zhang,Xiaoling Lou,Junxian Yun
A novel composite anion-exchange chitosan (CS) cryogel was synthesized via cryo-polymerization of CS and poly(butyl methacrylate) (pBMA) nanogels followed by graft polymerization with (vinylbenzyl)trimethylammonium chloride (VBTAC), which was then employed for the efficient separation of ovalbumin from egg white. Characteristics of the CS-pBMA-VBTAC cryogel were investigated experimentally, and the effects of sample volume, buffer pH and elution salt concentration on the ovalbumin separation were systematically evaluated. The results demonstrated that the present cryogel possessed an interconnected supermacroporous structure, high permeability, and excellent column efficiency. Notably, its adsorption capacity for bovine serum albumin reached 19.49 mg mL -1 , which is 4.4 and 5.6 times higher than that of VBTCA-grafted cryogels based on 2-hydroxyethyl methacrylate and acrylamide, respectively. Furthermore, the CS-pBMA-VBTAC cryogel maintained consistent adsorption performance over 20 runs of the replicate chromatographic separation of bovine serum albumin. Under optimal conditions of a loading volume equivalent to 6-fold column volumes, buffer pH 5.8 and elution with 0.8 M NaCl, the ovalbumin purity and recovery of 99.1 % and 96.1 %, were achieved, respectively, indicating that the CS-pBMA-VBTAC cryogel could be an excellent adsorbent material with promising potential for the high-efficiency separation of proteins like ovalbumin.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruihong Dong,Yuting Wang,David Julian McClements,Qiang Yu,Jianhua Xie,Huijie Zhang,Lizhi Huang,Bin Li,Jinlong Tian,Yi Chen
This study investigated intestinal absorption and cellular antioxidant efficacy of anthocyanin (ACN)-loaded ovalbumin (OVA)-carrageenans (ι-, λC)/dextran sulfate (DS) nanocomplexes. Utilizing Caco-2 cells, we assessed endocytic pathways, intracellular trafficking, and delivery efficacy of ternary nanocomplexes. ACN-loaded nanocomplexes could be internalized by cells in a time-, dose-, energy-dependent manner, with macropinocytosis, lipid raft/caveolae, and actin co-mediated endocytosis routes playing pivotal roles. Nanocomplexes inhibited P-glycoprotein efflux of Rhodamine-123, enhancing the apparent permeability of ACN into cells, especially for OVA-ιC-ACN. Transport pathways of ACN-loaded nanocomplexes through Caco-2 monolayers involved different organelles including microtubules, Golgi complexes, endoplasmic reticulum, and lysosomes. Compared to free ACN, nanocomplexes exhibited more efficiency in recovering cellular viability and endogenous antioxidant enzyme activities under oxidative stress. Our findings provide a more comprehensive understanding of internalization, transport mechanisms of protein-polysaccharide-anthocyanin nanocomplexes across epithelial cell monolayers, and provide valuable insights for designing efficient ACN-loaded nanocomplex systems with high transportation efficiency and intestinal absorption.
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FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Shan Qian,Lin Chen,Zhengshan Zhao,Xiaojing Fan,Xinglian Xu,Guanghong Zhou,Beiwei Zhu,Niamat Ullah,Xianchao Feng
Ovalbumin (OVA), is the most abundant protein in the egg white. In the present study, the effects of Epigallocatechin-3-gallate (EGCG) on the self-assembly behavior and gelling properties of OVA with different heating time were investigated. Electrophoresis , surface hydrophobicity, Fourier transform infrared (FTIR) spectroscopy and molecular docking analysis indicated that hydrophobic force and hydrogen bond were the predominant interactions between EGCG and OVA. The binding of EGCG to OVA suppressed the formation of fibrils as evidenced by the results of Thioflavin T (ThT) intensity and atomic force microscopy (AFM) analysis. When heated for 15–30 min, EGCG addition, especially at 2.5 μM/g, resulted in a decrease of ThT fluorescence intensity and hydrophobicity of OVA samples, and a formation of dense linear aggregates. However, the OVA solution did not form a gel. In a longer heating time of 45 min, 5 μM/g EGCG induced a significant decrease in ThT fluorescence intensity and hydrophobicity of OVA samples, and a transition from the dense linear aggregates to the dense spherical clusters. Therefore, the OVA samples realized the transition from the solution state to the gel state with a higher storage modulus (G′). When the heating time was extended to 60 min, all the OVA samples formed gel and reached the highest G′ at 5 μM/g EGCG. These results suggested that the suitable hydrophobic interactions and hydrogen bonds between EGCG and the OVA improved gel quality. Polyphenol binding provided a new way to regulate the assembly behavior and gelling properties of OVA.
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Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 46
·7703-7709
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IF 4.776
Min Qiao,Xun Liu,Jin-Wei Song,Ting Yang,Ming-Li Chen,Jian-Hua Wang
Tryptophan (Trp) modified aminated mesoporous silica nanoparticles (AMSNs), shortened to Trp-AMSNs, are prepared via covalent binding. The obtained Trp-AMSNs exhibit a uniform size of ca. 83 nm, a mesopore diameter of ca. 2.6 nm, along with a pore volume of 0.439 cm3 g−1. It is demonstrated that Trp-AMSNs selectively adsorb ovalbumin (Ova) from complex biological matrices. At pH 5.0, 1.0 mg of Trp-AMSNs produces an adsorption efficiency of 96% for 100 mg L−1 Ova in 1.0 mL of solution. An adsorption capacity of 1240.3 mg g−1 is derived for Ova, which is much improved with respect to that of the native AMSNs. The retained Ova could be readily recovered by a sodium dodecyl sulfate (SDS) solution (0.5%, m/v), providing a recovery of 71.2%. Trp-AMSNs are further applied for the isolation of Ova from a protein mixture (with a molar ratio of ovalbumin/lysozyme of 1 : 10) and an egg-white sample. High-purity Ova is obtained, as demonstrated by SDS-PAGE assay results.
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CELL BIOCHEMISTRY AND FUNCTION
·
2023
·Vol. 42
·期 1
·e3899
·
IF 3.6
Tingting Wang,Guihua Song,Mengmeng Sun,Yan Zhang,Bingxue Zhang,Minghao Peng,Mengyin Li
Asthma is a common respiratory disease associated with airway inflammation. Nerolidol is an acyclic sesquiterpenoid with anti-inflammatory properties. BALB/C mice were sensitized with ovalbumin (OVA) to induce asthma symptoms and given different doses of Nerolidol. We found that Nerolidol reduced OVA-induced inflammatory cell infiltration, the number of goblet cells and collagen deposition in lung tissue. Nerolidol reduced the OVA-specific IgE levels in serum and alveolar lavage fluid in an asthma model. Immunohistochemical staining of α-SMA (the marker of airway smooth muscle) showed that Nerolidol caused bronchial basement membrane thinning in asthmatic mice. The hyperplasia of airway smooth muscle cells (ASMCs) is an important feature of airway remodeling in asthma. ASMCs were treated with 10 ng/mL TGF-β to simulate the pathological environment of asthma in vitro and then treated with different doses of Nerolidol. Nerolidol inhibited the activity of TGF-β/Smad signaling pathway both in the lung tissue of OVA-induced mouse and TGF-β-stimulated ASMCs. 16s rRNA sequencing was performed on feces of normal mice, the changes of intestinal flora in OVA-induced asthmatic mice and Nerolidol-treated asthmatic mice were studied. The results showed that Nerolidol reversed the reduced gut microbial alpha diversity in asthmatic mice. Nerolidol changed the relative abundance of gut bacteria at different taxonomic levels. At the phylum level, the dominant bacteria were Bacteroidota, Firmicutes, and Proteobacteria. At the genus level, the dominant bacteria were Lactobacillus, Muribaculaceae, Bacteroides , and Lachnospiraceae . We conclude that Nerolidol attenuates OVA-induced airway inflammation and alters gut microbes in mice with asthma via TGF-β/Smad signaling. Significance statement This research has demonstrated a mitigating effect of Nerolidol on the progress of asthma. TGF-β/Smad signaling pathway is the critical target of Nerolidol. Intestinal flora disorders induced by asthma are also regulated by Nerolidol.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ji-hua Mao,Kai Zhang,Ying-fei He,Jun Liu,Yan-hong Shao,Zong-cai Tu
Ovalbumin (OVA) was modified by fructose (Fru) and galactose (Gal) to study the structure, IgG/IgE binding capacity and effects on human intestinal microbiota of the conjugated products. Compared with OVA-Fru, OVA-Gal has a lower IgG/IgE binding capacity. The reduction of OVA is not only associated with the glycation of R84, K92, K206, K263, K322 and R381 in the linear epitopes, but also with conformational epitope changes, manifested as secondary and tertiary structural changes caused by Gal glycation. In addition, OVA-Gal could alter the structure and abundance of gut microbiota at phylum, family, and genus levels and restore the abundance of bacteria associated with allergenicity , such as Barnesiella , Christensenellaceae_R-7_group , and Collinsela , thereby reducing allergic reactions. These results indicate that OVA-Gal glycation can reduce the IgE binding capacity of OVA and change the structure of human intestinal microbiota. Therefore, Gal glycation may be a potential method to reduce protein allergenicity.
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RSC Advances
·
2022
·Vol. 12
·期 28
·18115-18126
·
IF 4.036
Xiaoya Tao,Hang Shi,Ailing Cao,Luyun Cai
In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare an OVA–SA composite carrier, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA) (OVA–SA, OVA–TA–SA, OVA–KAE–SA, and OVA–TA–KAE–SA). Results showed that the observation of small diffraction peaks in carriers proved the successful encapsulation of KAE/TA. The protein conformation of the composite nanoparticles changed. OVA–TA–SA composite nanoparticles had the highest α-helix content and the fewest random coils, so the protein structure of it had the strongest stability. OVA–TA–KAE–SA composite nanoparticles had the strongest system stability and thermal stability, which might be due to the synergistic effect of the two polyphenols, suggesting the encapsulation of KAE/TA increased the system stability and the thermal stability of OVA–SA composite nanoparticles. Additionally, the composite nanoparticles were endowed with antioxidant ability and antibacterial ability (against Staphylococcus aureus and Escherichia coli) in the order OVA–TA–SA > OVA–TA–KAE–SA > OVA–KAE–SA based on the difference in antibacterial diameter (D, mm) and square (S, mm2), indicating that polyphenols enhanced the antibacterial and antioxidant ability of OVA–SA composite nanoparticles, and the enhancement effect of TA was stronger than that of KAE. These results provide a theoretical basis for the application of OVA–SA composite nanoparticles in the delivery of bioactive compounds.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 9
·3835-3846
·
IF 3.639
Lei Chen,Miao Zhu,Xing Hu,Junhui Pan,Guowen Zhang
BACKGROUND Ferulic acid (FA), a phenolic acid widely occurring in nature, has attracted extensive attention because of its biological activity. Ovalbumin (OVA) is a commonly used carrier protein. The mechanism of FA binding with OVA was investigated by utilizing a variety of spectral analyses, accompanied by computer simulation. RESULTS It was discovered that the fluorescence quenching mechanism of OVA by FA was a static mode as a result of the formation of an FA−OVA complex, which was verified by the concentration distributions and pure spectrum of the constituents decomposed from the high overlap spectrum signals using multivariate curve resolution–alternate least squares algorithm. Hydrogen bonds and Van der Waals forces drove the formation of FA–OVA complex with a binding constant of 1.69 × 10 4 L mol −1 . The presence of FA induced the loose structure of OVA with an attenuation of α-helix content and improved the thermal stability of OVA. Computer docking indicated that FA interacted with the amino acid residues Arg84, Asn88, Leu101 and Ser103 of OVA through hydrogen bonds. Molecular dynamics simulation proved that the combination of FA with OVA boosted the conformational stability of OVA and hydrogen bonds brought a crucial part in stabilizing the structure of the complex. CONCLUSIONS The study may supply the theoretical basis for the design of FA transport system using OVA as carrier protein to improve the instability and low bioavailability of FA. © 2021 Society of Chemical Industry.
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International Journal of Nanomedicine
·
2020
·
IF 5.115
Mingzhuo Cao,Mengling Zhan,Zheng Wang,Zeqian Wang,Xiu-Min Li,Mingsan Miao
Purpose Isoliquiritigenin (ILQ), an important component of Anti-Asthma Herbal Medicine Intervention (ASHMI), had shown potent anti-asthma effect in vitro in our previous study. However, poor solubility and low bioavailability hindered in vivo application to treat asthma. This study was to develop a novel ILQ loaded self-nanoemulsifying drug delivery system (ILQ-SMEDDS) with enhanced bioavailability. Methods The optimized SMEDDS formulation was composed of ethyl oleate (oil phase), Tween 80 (surfactant) and PEG400 (co-surfactant) at a mass ratio of 3:6:1. The physiochemical properties of ILQ-SMEDDS, including drug content, globule size, zeta potential, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, were characterized. And the in vitro release profile, in situ intestinal absorption, in vivo pharmacokinetic parameters and the anti-asthma effect of ILQ suspension and ILQ-SMEDDS were evaluated. Results The ILQ-SMEDDS had an average globule size of 20.63 ± 1.95 nm with a polydispersity index (PDI) of 0.11 ± 0.03, and its zeta potential was −12.64 ± 2.12 mV. The cumulative release rate of ILQ from ILQ-SMEDDS to the simulated gastrointestinal tract was significantly higher than that of free ILQ suspension. And area under curve with ILQ-SMEDDS was found to be 3.95 times higher than that of ILQ suspension indicating improved bioavailability by SMEDDS. Although ILQ-SMEDDS showed a slight less effective inhibitory effect on eotaxin-1 in human lung fibroblast (HFL-1) cells than free ILQ, in an ovalbumin-induced asthma model, ILQ-SMEDDS exhibited more efficacy than ILQ suspension in improving asthma-associated inflammation, including eosinophil production, ovalbumin-specific immunoglobulin E (OVA-sIgE), interleukin 4 (IL 4), interleukin 5 (IL 5) and interferon-γ (IFN-γ). Even the low dose of ILQ-SMEDDS group (10 mg/kg) showed better anti-asthma effect than that of the ILQ suspension group (20 mg/kg). Conclusion Compared with ILQ suspension, ILQ-SMEDDS showed significantly improved bioavailability and anti-asthma effect, revealing its potential as a favorable pharmaceutical agent for treating asthma.
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