INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Linyun Mou,Ya Lu,Jiale Ma,Shuwei Shu,Jianlong Li,Ganpeng Li
In response to the rapid quality degradation of peaches due to post-harvest decay, this study developed a composite film for preservation. Two primary pathogens responsible for peach decay, Aspergillus flavus and Talaromyces funiculosus , were identified. Molecular docking attributed to the main binding position of citral and geraniol's interactions with type I polyketide synthase on their antifungal effects, as well as the caffeic acid with its tyrosinase on antioxidant efficacy. β -CD-MOFs were synthesized via vapor diffusion, demonstrating exceptional spatial loading via SEM. CEOs (citral + geraniol) encapsulated in β -CD-MOFs were incorporated into a chitosan/pullulan matrix, yielding a film with 260.27 % fracture tensile strain, significantly ( P < 0.001) outperforming pure chitosan (21.87 %), possessing excellent mechanical properties, and the improvement in the water solubility of the film facilitates the release of active compounds. The utilization of a compositel film on peaches has shown significant efficacy in controlling decay index ( P < 0.05), weight loss ( P < 0.05), firmness ( P < 0.05) and production of malondialdehyde ( P < 0.05). Compared to untreated controls, the treated peaches exhibited an extended shelf life of 2 days, highlighting the superior preservation capabilities of the film. Hence, the film presents a promising solution for fruit preservation.
研究领域:
食品科学
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材料科学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2021
·
IF 4.034
Yi Pan,Xiao-Min Li,Ran Meng,Bao Zhang
ABSTRACT The effects of maleic anhydride (MA) acylation and pullulan glycation on casein hydrolysates (CH) and the physicochemical stability of modified or unmodified CH-stabilized emulsions were explored. Compared with casein, the solubility of CH was improved, and CH 1 (hydrolysis degree 4%) exhibited the optimal emulsifying properties. After the acylation of MA, degrees of acylation (DA) increased with increasing addition of MA. Fourier-transform infrared spectroscopy revealed that a covalent bond was formed between MA and CH 1 . The results of pullulan glycation indicated that the degree of glycation decreased with increasing DA. Acylation combined with glycation effectively reduced the surface hydrophobicity of CH. Results of analysis of physicochemical stability and gastrointestinal fate of curcumin in emulsions revealed that CH modified by MA acylation and pullulan glycation played a positive role in enhancing the stability and bioaccessibility of curcumin loaded in emulsions.
研究领域:
食品科学
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化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2019
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IF 3.074
Kunhua Lin,Juzhen Yi,Xuhong Mao,Hao Wu,Li-Ming Zhang,Liqun Yang
Pullulan , which is a linear glucan with non-toxicity, good biocompatibility and biodegradability , was carboxylated with succinic anhydride to yield a pullulan derivative bearing COOH groups (CPUL). The CPUL structure was determined by Fourier-transform infrared (FTIR) and 1 H nuclear magnetic resonance (NMR) spectroscopies. 1 H NMR integration showed that the degree of substitution of COOH residues was 0.2. A covalently modified concanavalin A (ConA)-CPUL hydrogel with glucose sensitivity was prepared by an amidization reaction between the COOH group of CPUL and the NH 2 group of ConA via a safe and mild 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/ N -hydroxysuccinimide activation method. Insulin-loaded CPUL-ConA (insulin/CPUL-ConA) hydrogels were prepared by absorption of insulin in situ . Analysis of the release profiles in vitro confirmed that smart controlled release of insulin from the insulin/CPUL-ConA hydrogels could be achieved because of specific ConA-glucose binding. The insulin/CPUL-ConA hydrogels showed the characteristic viscoelasticity and shear thinning properties of covalently modified hydrogels. Scanning electron microscopy showed that the hydrogels had crosslinked network structures containing uniform pores and insulin particles. This work provides a pullulan-based glucose-sensitive hydrogel that enables smart controlled release of insulin in response to changes in glucose concentration. Such hydrogels could be used in closed-loop systems for diabetes therapy.
研究领域:
生物材料学
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药物递送系统
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糖尿病治疗
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产品关联
DOI
高影响因子
MATERIALS TECHNOLOGY
·
2022
·
IF 3.297
Xin Tian,Xiangling He,Anqi Yao,Yalan You,Fan Yang,Keke Chen,Chengguang Zhu,Minhui Zeng,Qin Shi,Wenfang Tang,Xuelong Fan,Xiaojun Tao
Hydrophobically modified amino-pullulan polymers (CHPNH2) were modified with polyethylene glycol (PEG) through the connective arms of succinic anhydride (SA) and cis aconite anhydride (CA) to form three polymers, namely, PCCN1, PCCN2 and PSCN, respectively. The structure of the polymer was identified by 1H NMR, and the degree of substitution (DS) values of hydrophobic groups were calculated. Three types of nanoparticles (NPs) were prepared by the dialysis method, and the average particle sizes by dynamic light scattering (DLS) were 166.9 nm, 118.9 nm and 118.7 nm. The drug release experiment showed that the three drug-loaded nanoparticles all had a slow-release effect. The results of the MTT experiment showed that the cell survival rates of free cisplatin and the three types of drug-loaded nanoparticles were 42.30%, 40.17%, 37.53% and 53.34% after 48 h at the maximum drug concentration, indicating that PCCN1 nanoparticles have the strongest cytotoxicity. They also had the highest cell uptake rate in cell uptake experiments .
研究领域:
药学
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纳米技术
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癌症研究
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 13
·6928
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IF 6.208
Meixing Wang,Huizhen Hu,Buyu Zhang,Yang Zheng,Pan Wu,Zhenghui Lu,Guimin Zhang
Panose is a type of functional sugar with diverse bioactivities. The enzymatic conversion bioprocess to produce high purity panose with high efficiency has become increasingly important. Here, a new neopullulanase (NPase), Amy117 fromB. pseudofirmus703, was identified and characterized. Amy117 presented the optimal activity at pH 7.0 and 30 °C, its activity is over 40% at 10 °C and over 80% at 20 °C, which is cold-active. The enzyme cleaved α-1, 4-glycosidic linkages of pullulan to generate panose as the only hydrolysis product, and degraded cyclodextrins (CDs) and starch to glucose and maltose, with an apparent preference for CDs. Furthermore, Amy117 can produce 72.7 mg/mL panose with a conversion yield of 91% (w/w) based on 80 mg/mL pullulan. The sequence and structure analysis showed that the low proportion of Arg, high proportion of Asn and Gln, and high α-helix levels in Amy117 may contribute to its cold-active properties. Root mean square deviation (RMSD) analysis also showed that Amy117 is more flexible than two mesophilic homologues. Hence, we discovered a new high-efficiency panose-producing NPase, which so far achieves the highest panose production and would be an ideal candidate in the food industry.Keywords:neopullulanase (NPase);pullulan;panose production;high-efficiency
研究领域:
食品科学
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酶学
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生物技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Guoyu Cao,Nitong Bu,Taotao Zeng,Runzhi Sun,Ruojun Mu,Jie Pang,Lin Wang
In this work, konjac glucomannan (KGM)-based film reinforced with pullulan (PL) and acai berry extract (ABE) was developed by solvent casting method. The as-prepared films performed pH-sensitive properties, which can be potentially applied for fish freshness detection. Rheology , Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) were used to characterize chemical structure and morphology of ABE-loaded KGM/PL (KP) films (KP-ABE). FT-IR spectrum indicated that hydrogen bond dominated the formation of KP-ABE films. Adding PL contributed to enhanced mechanical properties of KGM film with increased tensile strength (TS) from 21.25 to 50.27 MPa and elongation at break (EAB) from 10.64 to 19.19 %. Incorporating ABE upgraded flexibility, UV-shielding, thermostability , water barrier (decreased Water vapor permeability (WVP) from 2.07 to 1.67 g·mm/m 2 ·day kPa), antioxidant, and antibacterial ability of KP films, but weakened TS. In addition, KP-ABE films can reflect fish freshness in real time through color variability. Therefore, KP-ABE films exhibited potential applications in intelligent food packaging materials.
研究领域:
食品科学
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材料科学
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化学
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生物材料
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包装工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 48
·51553
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IF 3.125
Ju-Zhen Yi,Kunhua Lin,Hao Wu,Xuhong Mao,Li-Ming Zhang,Liqun Yang
A hydrogel comprising covalently modified carboxylated pullulan and concanavalin A (CPUL-ConA) was prepared with glucose-sensitivity in a simulated small intestinal fluid containing glucose. The acarbose-encapsulated CPUL-ConA (Acar/CPUL-ConA) hydrogel, with an acarbose encapsulation ratio of 0.63, was prepared by immersing the dried CPUL-ConA hydrogel in an acarbose/PBS (pH 7.2) solution. In the simulated gastric fluid, less than 20% acarbose was released from the Acar/CPUL-ConA hydrogel, which could be beneficial to protecting the stomach. In contrast, more than 80% acarbose was smartly controlled released from the Acar/CPUL-ConA hydrogel in the simulated small intestine fluid containing glucose. Scanning electron microscopy provided further evidence of the smart release of acarbose from the Acar/CPUL-ConA hydrogel in simulated gastrointestinal fluids, which is advantageous to inhibiting α-glucosidase activity and subsequently reducing the postprandial blood glucose levels, as well as helpful for reducing the gastrointestinal dysfunction of acarbose. Rheological analysis indicated that the Acar/CPUL-ConA hydrogel showed characteristics of chemical crosslinked hydrogels. There was no obvious shear thinning behavior, suggesting that the Acar/CPUL-ConA hydrogel could be stable under gastric and intestinal peristalsis. Therefore, the Acar/CPUL-ConA hydrogel is anticipated to improve the therapeutic efficacy of postprandial hyperglycemia and type 2 diabetes.
研究领域:
药学
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材料科学
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生物医学工程
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药物递送系统
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xing Zhao,Haifei Guo,Hriday Bera,Huiyang Jiang,Yang Chen,Xiong Guo,Xidong Tian,Dongmei Cun,Mingshi Yang
Paclitaxel (PTX) remains a cornerstone in the treatment of locally advanced and metastatic lung cancer. To improve its therapeutic indices against lung cancer, novel redox-sensitive pullulan/PTX-based prodrug NPs (PULL–SS–PTX NPs) were accomplished, which were further surface-decorated with transferrin (TF), a cancer cell-targeting ligand, to afford TF–PULL–SS–PTX NPs. These prodrug NPs (drug content, >37% and average size, 134–163 nm) rapidly dismantled their self-assembled architecture upon exposure to simulated reducing conditions, causing a triggered drug release as compared to the control scaffold (PULL–CC–PTX NPs). These scaffolds also evidenced outstanding colloidal stability, cellular uptake efficiency, and discriminating cytotoxicity between the cancer and healthy cells. Intravenously delivered redox-sensitive NPs exhibited improved tumor-suppressing properties as compared to the control nanovesicles (PULL–CC–PTX NPs) in a B16–F10 melanoma lung metastasis mice model. The targeting efficiency and associated augmented anticancer potentials of TF–PULL–SS–PTX NPs relative to TF-free redox-responsive NPs and Taxol intravenous injection were also established on the transferrin receptor (TFR) overexpressed Lewis lung carcinoma (LLC–luc) cell-bearing mice model. Moreover, the TF-functionalized scaffold displayed a reduced systemic toxicity compared to that of Taxol intravenous injection. Overall, the proposed TF-decorated prodrug NPs could be a promising nanomedicine for intracellular PTX delivery against metastatic lung cancer.
研究领域:
药学
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纳米技术
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肿瘤学
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Mengxia Duan,Jishuai Sun,Shan Yu,Zijian Zhi,Jie Pang,Chunhua Wu
Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria. However, it is susceptible to the external environment, i.e. temperature, pH, and food composition. In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated. The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents. The result revealed that the smooth surface can be observed on the nanofibers by microstructure characterization. The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity. Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days. Taken together, the CNNGs can be well stabilized with the core-shell nanofibers, thus exerting significantly improved antimicrobial stability and bioactivity. This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
研究领域:
材料科学
·
食品科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Min Zhang,Bin Yang,Zimeng Yuan,Qi Sheng,Changchun Jin,Jun Qi,Manman Yu,Yingnan Liu,Guoyuan Xiong
The present study investigated the mechanical characteristics, hydrophobicity, antioxidant and antibacterial properties, FTIR, SEM and XRD of films fabricated with corn starch and pullulan (CS/PUL) by adding different concentrations of Gallic acid (GA) (0%, 0.5%, 1.0%, 1.5% w/v). The mechanical strength and opacity of CS/PUL films were enhanced by the addition of 1.0% GA. The water vapor permeability (WVP) of CS/PUL films was significantly lower in films with GA compared to those without ( P < 0.05). The addition of GA, especially at concentrations of 1.0% and 1.5%, resulted in considerably better free radical scavenging activities on DPPH than films without GA ( P < 0.05). Interestingly, the highest water contact angle (WCA) value was observed in films with 0.5% GA, indicating stronger hydrophobicity. Furthermore, the antibacterial capabilities of the films, particularly against E. coli and P. aeruginosa , improved with an increase in GA concentration. The results of FTIR, SEM and XRD analyses showed that GA was well distributed in the CS/PUL matrix.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Shuyan Zhang,Xuxin Zhang,Yingyin Wu,Yinyin Shi,Xiaocui Wang,Jianfeng Wu,Xu Chen,Yujia Liu,Siqian Chen,Jie Zhu
Thymol/waxy maize starch nanoemulsion (TWSN) was successfully prepared via dynamic high-pressure micro-fluidization (DHPM) and applied in pullulan-based active food packaging material (P-TWSN). The properties of TWSN and its effect on the structural and physical-chemical properties of P-TWSN were then investigated. Concurrently, the impact of TWSN (0, 5, 15, 20, 25 mL) on the storage quality and flavor substances of strawberries coated by film-forming solution (FFS) were investigated by gas chromatography-ion mobility spectrometry (GC-IMS). The results demonstrated that the prepared TWSN exhibited remarkable storage stability, maintaining a particle size of 127.9 ± 1.02 nm and a ζ-potential of −32.4 ± 0.42 mV. As TWSN concentration increased, P-TWSN exhibited a smooth and compact morphology, characterized by the presence of bubble-like pores and granular protrusions. Additionally, the FTIR peaks corresponded to the stretching vibration of -OH group of TWSN and related materials were shifted to lower wavenumbers. Furthermore, the materials with increasing content of TWSN exhibited excellent water solubility, enlarged WVP, stable thermal property and superior antibacterial capacity against gram-positive ( S.aureus ) than gram-negative ( E. coli ) bacteria. Strawberry samples sprayed by FFS with 20 mL TWSN exhibited the slowest decline in shrinkage, weight loss, titrable acidity, highest value of firmness, total soluble solids, and lower content of ascorbic acid and viable microorganisms in comparison with other dosages. Additionally, the contents of esters and alcohols in treated strawberries reached the summit on the fourth day of storage and subsequently declined, while the ketone content exhibited a downward trend with increasing TWSN, particularly in the samples with 20 and 25 mL TWSN. The studies identified an efficient method for preparing starch-based nanoemulsion by DHPM, which could be prospective in bio-active packaging materials.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wenzhuang Cui,Chu Gong,Yujie Liu,Ying Yue,Jun Wang,Zhizhou Yang,Junli Yang
Antibacterial hydrogels as burn wound dressings are capable of efficaciously defending against bacterial infection and accelerating burn wound healing. Thus far, a large plethora of antibacterial hydrogels have adopted numerous components and intricate preparation processes, yet restricting their practical industrialization applications. Simple and effective preparation methods of antibacterial hydrogels are hence urgently needed. Herein, an easy but efficacious strategy with the employment of two natural products pullulan and ε-poly- l -lysine (ε-PL) was designed to fabricate composite antibacterial hydrogels for burn wound healing for the first time. The hydrogel crosslinking networks were formed through amidation reactions between carboxylated pullulan derivative (CP) and ε-poly- l -lysine hydrochloride (ε-PL·HCl). The resulting hydrogels possessed high transparency, porous structures, tunable gelation time and gel content, relatively low swelling ratios, appropriate self-degradability, proper mechanical properties, strong in vitro bacteriostatic activities, non-cytotoxicity, capacities of facilitating cell migration and excellent hemocompatibility. In the infected burn model of mice, the hydrogels were observed to display prominent in vivo antibacterial activities and enable the acceleration of burn wound healing. We opine the simply and effectively prepared antibacterial hydrogels as promising dressings for burn wound recovery have broad industrialization prospects.
研究领域:
材料科学
·
生物医学工程
·
药剂学
·
伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）