INFLAMMATION
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2022
·Vol. 45
·期 3
·1362-1373
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IF 4.657
Ji Xiang-Fen,Fan Yu-Chen,Sun Fei,Wang Jing-Wei,Wang Kai
Acute liver failure (ALF) is a deadly clinical disorder with few effective treatments and unclear pathogenesis. In our previous study, we demonstrated that aberrant Wnt5a expression was involved in acute-on-chronic liver failure. However, the role of Wnt5a in ALF is unknown. We investigated the expression of Wnt5a and its downstream c-Jun N-terminal kinase (JNK) signaling in a mouse model of ALF established by coinjection of D-galactosamine (D-Gal) and lipopolysaccharide (LPS) in C57BL/6 mice. We also investigated the role of Box5, a Wnt5a antagonist, in vivo. Moreover, the effect of Wnt5a/JNK signaling on downstream inflammatory cytokine expression, phagocytosis, and migration in THP-1 macrophages was studied in vitro. Aberrant Wnt5a expression and JNK activation were detected in D-Gal/LPS-induced ALF mice. Box5 pretreatment reversed JNK activation and eventually decreased the mortality rate of D-Gal/LPS-treated mice, with reduced hepatic necrosis and apoptosis, serum ALT and AST levels, and liver inflammatory cytokine expression, although the latter was not significant. We further demonstrated that recombinant Wnt5a (rWnt5a)-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA expression and increased THP-1 macrophage phagocytosis in a JNK-dependent manner, which could be restored by Box5. In addition, rWnt5a-induced migration of THP-1 macrophages was also reversed by Box5. Our findings suggested that Wnt5a/JNK signaling plays an important role in the development of ALF and that Box5 could have particular hepatoprotective effects in ALF.
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高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
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2022
·Vol. 30
·期 7
·2774-2784
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IF 4.705
Yan Zhuofeng,Zhu Kaixiao,Li Xiangqi,Wu Xiao
Bacterial cellulose (BC) aerogel has great potential in treating oil spill and organic pollutant. However, its inherent hydrophilicity and poor rigidity limit its practical application and recyclability. In this study, elastically compressible and high oil-absorbing aerogels were developed by freeze-drying aqueous suspensions with appropriate BC concentrations, followed by a chemical vapor deposition of methyltrimethoxysilane with ammonia as catalyst. The modified aerogel shows high water contact angle of 142° and enhanced compression resistance. The effect of BC concentration on the absorption capacity and recyclability of aerogel has been investigated. The results show that the aerogel prepared with 0.3 wt% BC exhibits simultaneously high absorption capacity (121.8–284.1 g/g) and excellent recyclability. Futhermore, the aerogel could also separate chloroform-water mixture by gravity-driven filtration, giving the separation efficiency of 96.7%. Therefore, this economical green aerogel provides a feasible strategy for solving oil leakage in industry.
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CHEMICAL PAPERS
·
2022
·Vol. 76
·期 6
·3937-3947
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IF 2.146
Li Xinxin,Lin Min,Zhang Hongfeng,Ji Wenquan,Shi Yuhan,Qi Zhiyang,Fu Tianwen,Li Qinran,Deng Qiliang
A kind of surface imprinted particles was synthesized to recognize cytochrome c (Cyt c) by a novel strategy combining reversible addition-fragmentation chain transfer (RAFT) radical polymerization and epitope surface imprinting. N-terminal epitope nonapeptide of Cyt c was chosen as the template. The selectivity of these particles for protein recognition was obviously improved due to the selection of epitopes rather than the whole protein as templates and the controllable length of polymer shell chain. After epitope had been modified on the surface of silica, the surface imprinted polymer with controlled imprinted layer was synthesized with monomers and cross-linkers by RAFT radical polymerization strategy. The epitope surface imprinted particles were obtained after the peptide removed and trithioester group destruction by hexylamine. The binding capacity of N-terminal peptide reached 1.88 mg peptide per gram with imprinting factor (IF) as 2.42 while the binding capacity of Cyt c reached 8.89 mg protein per gram with IF as 1.71, which were obviously higher than the IF of the material without RAFT strategy. Moreover, Cyt c could be selectively recognized by the epitope surface imprinted particles even in presence of the competitive proteins with different molecular weight and isoelectric point. The performance of protein recognition remained 90% after five cycles of adsorption and desorption. All these results demonstrated that the epitope surface imprinted particles prepared by RAFT strategy are promising to achieve the protein recognition with higher recognition ability, selectivity and reusability.
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ARCHIVES OF GYNECOLOGY AND OBSTETRICS
·
2022
·Vol. 306
·期 1
·115-125
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IF 2.493
Zhu Bo,Chen Yumei,Guo Min,zhang Chenhui,Huang Lingxiao,Pan Qiangwei,Lin Tao,Lu Yiyi,Shen Xiaolu,Zhang Hongping
Purpose Adenomyosis is a common gynecological disease, but its pathogenesis and treatment options are not yet completely clear. This study aimed to investigate the analgesic effect of berberine on tamoxifen-induced neonatal mouse adenomyosis and its curative effects on the disease. Methods The mouse adenomyosis model was established in neonatal female mice via oral administration of tamoxifen suspended solution. Adenomyosis mice were given berberine by intraperitoneal injection with the dosage of 5, 10, and 20 mg/kg body weight, respectively, at 17 weeks after birth. The pain sensation of the mice was evaluated by hotplate and tail-flick tests. The mRNA levels of gene expression were detected by RT-qPCR. The protein expression was analyzed by ELISA and Western blot. Results Berberine reduced the uterine weight, suppressed the myometrial infiltration of ectopic endometrium, improved the hotplate and tail-flick latency of the adenomyosis mice. Mechanistically, berberine downregulated the expression of genes related to pain and inflammation, such as TRPV1, COX-2, VEGF and OTR, impaired the inflammatory response at the DRG site, and inhibited the expression of TLR4 in DRG and uterine tissues. Conclusions Berberine attenuates hyperalgesia and exhibits analgesic and therapeutic effects on adenomyosis mice.
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高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 4
·2553-2563
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IF 6.123
Luo Ruiqing,Liu Ruihua,Li Yongqiang,Li Yarong,He Chiyang
The main challenge in developing polymer/covalent organic framework (COF) hybrid matrix membrane is to find effective strategies for strongly attaching crystalline COF to the supporting surface as defect-free membrane layers. In this article, we demonstrated for the first time a method for the preparation of a chitosan (CS)/COF mixed matrix dual-layer membrane based on solvothermal synthesis method. A novel surface modification strategy was developed using p-phthalaldehyde as covalent linkers between COF and CS membrane substrate, and then the nitrogen-containing covalent organic framework (NCOF) membrane was further grown in situ on the modified CS membrane substrate utilizing solvothermal synthesis methods. The resultant CS/NCOF dual-layer membrane exhibited highly homogeneous texture due to the excellent compatibility between NCOF crystals and CS matrixes. The combination of NCOF and CS membrane formed a large number of channels in the nitrogen environment, enabling the CS/NCOF dual-layer membrane to have an enhanced iodine uptake capacity up to 3.15 g/g. Furthermore, the CS/NCOF dual-layer membrane could be recycled at least five times while maintaining 95% iodine capture capacity. These properties make the obtained CS/NCOF dual-layer membrane ideal absorbent material for reversible iodine capture. This ingenious dual-layer membrane preparation strategy provides a new avenue to customize synthesis of novel membrane materials for clean energy and environmental remediation. Graphical abstract
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BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2022
·Vol. 27
·期 1
·135-144
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IF 3.386
Wang Zichen,Liu Yang,Li Jinhong,Meng Guoqing,Zhu Daoyu,Cui Jiandong,Jia Shiru
As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH 2 is very low due to small pore size and few amino groups on the surface of the MIL-125-NH 2 . In this work, catalase (CAT) was immobilized on the MIL-125-NH 2 and amino functionalized MIL-125-NH 2 by adsorption (CAT@MIL-125-NH 2 ) and covalent binding (CAT@Amino MIL-125-NH 2 ), respectively. Compared with the CAT@MIL-125-NH 2 and free CAT, the CAT@Amino MIL-125-NH 2 displayed high activity recovery, good pH stability, stability against denaturants, and thermostability. Furthermore, activity recovery of CAT@Amino MIL-125-NH 2 was 56% higher than CAT@MIL-125-NH 2 . The CAT@Amino MIL-125-NH 2 still retained 50% residual activity for 14 days at room temperature, whereas free CAT lost activity after storage for 1 day at the same storage conditions. Furthermore, the CAT@Amino MIL-125-NH 2 maintained 62% of its initial activity after 4 consecutive uses, showing good reusability. The results showed that the amino functionalized MIL-125-NH 2 is an excellent carrier of enzyme immobilization.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2022
·Vol. 33
·期 7
·3965-3981
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IF 2.779
Ahmed Arsalan,Jia Yunming,Deb Hridam,Arain Muhammad Fahad,Memon Hafeezullah,Pasha Khalid,Huang Yi,Fan Qinguo,Shao Jianzhong
The use of piezoelectric materials has been increased due to the growing demand for wearable devices. Herein, we report the development of new copolymer poly (vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE)-based reduced graphene oxide (rGO) and multiwalled carbon nanotubes (MCNTs) loaded electrospun (E-Spun) nanofibers. The rGO-MCNTs loaded PVDF-TrFE nanofibrous mat led to the fabrication of ultra-sensitive piezoelectric pressure sensors for potential wearable health monitoring applications. The doped PVDF-TrFE solution of different weight percentages of rGO-MCNTs as fillers was prepared and used to fabricate an e-spun nanofibrous mat. Complete characterization of resultant materials were carried through diverse instruments which reveals the successful integration of rGO-MCNTs (3.2%) as a dopant that improved the β-phase up to 92%. The DSC analysis further exposes the high thermal stability of the PVDF-TrFE nanofibers mat due to the enhanced crystallinity with the addition of nanofillers. The newly developed sensor’s overall output (based on sensitivity) was calculated under a variable applied pressure range of 0.25 ~ 300 cN at 50 HZ. Results show that the pressure sensor response has improved from 16.125 ~ 0.430 kPa −1 , corresponding to a higher sensitivity under static and dynamic forces in the applied pressure range. These results are of a fundamental study and open new prospects for the hybrid nanofibrous mat as alternative electrode material in the piezoelectric pressure sensor.
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高影响因子
Journal of Central South University
·
2022
·Vol. 29
·期 2
·397-409
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IF 2.392
Xu Wen-shuang,Yang Zhou-xiao-shuang,Zhang Guang-yang,Liu Hui
A light and temperature dual responsive copolymer, poly(7-(4-vinylbenzy-loxyl) -4-methylcoumarin-co-N-vinyl caprolactam-co-tri(ethylene glycol)methyl ether methacrylate) (PVNM), was grafted on the surface of dopamine-based mesoporous silica nanoparticles (MSNs). The resulting polymer brush, MSNs-g-PVNM, was characterized by FT-IR, TEM, TGA and XPS. The dual responsive behaviors of MSNs-g-PVNM were systematically studied. With imidacloprid as the model guest pesticide, the loading percentage and loading efficiency of the polymer brush were determined as 9.2% and 40.6%, respectively. The release efficiency of imidacloprid in MSNs-g-PVNM was the lowest value of 5.4% at 20 °C and 365 nm, and it reached the highest value of 52.4% at 50 °C and 254 nm. The loss percentage of imidacloprid on the leaves contained imidacloprid-loaded MSNs-g-PVNM (8.4%) was much less than that contained only imidacloprid (25.2%) after three rinses. It was confirmed that the release process of imidacloprid was well regulated through changing external conditions such as light and temperature.
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高影响因子
npj Flexible Electronics
·
2022
·Vol. 6
·期 1
·1-8
·
IF 12.019
Li Xin,Zou Ruike,Liu Zhen,Mata Jitendra,Storer Ben,Chen Yu,Qi Weiheng,Zhou Zekun,Zhang Peng
In this work, a polymer and ionic liquid (IL) hybrid with superior thermoelectric performance is prepared via a system design of the chemical composition, molar ratio of the constituent molecules and manipulating the structure in solution and dried films. The solution-casted hybrid film, consisting of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and 1-ethyl-3-methylimidazolium tricyanomethanide (EMIM:TCM), shows the highest power factor of 175 μW m −1 K −2 in the polymer hybrid prepared by a post-treatment-free method. With a set of complementary structure characterization methods, it is found that EMIM:TCM can induce the structure reorganization of PEDOT:PSS in solution from a core-shell model to a rod-like model, during which PEDOT partially separates from PSS that eases the conductive network formation. In addition, the oxidation level of PEDOT:PSS is reduced by adding EMIM:TCM. Based on which, the PEDOT:PSS/IL hybrid shows the best performance in optimizing the conductivity (1163 S cm −1 ) and Seebeck coefficient (38.8 μV K −1 ) simultaneously.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 2
·1-12
·
IF 6.408
Wang Dong-En,You Shangqi,Huo Wenjing,Han Xiang,Xu Huiyun
A colorimetric assay based on polydiacetylenes (PDA) nano-liposomes is reported for facile and sensitive detection of alkaline phosphatase (ALP) activity. The critical basis of this method is that the interaction of pyridoxal phosphate (PLP) with nitrogenous group functionalized PDA nano-liposomes induces distinct blue-to-red color changes of PDA nano-liposomes. In the presence of ALP, as a nature substrate, PLP is enzymatically hydrolyzed to form pyridoxal, which cannot interact with PDA nano-liposomes. As a result, the concentration of PLP is reduced and the color change of PDA nano-liposomes is retarded, which is associated with ALP level. Under optimal conditions, the proposed method showed good linear relationship with ALP activity in the range 10–200 U/L with a limit of detection of 2.8 U/L. The detection process could be vividly observed with the naked eye. Additional attempts by using the method for the evaluation of inhibitor efficiency were also achieved with satisfying results. The method was further challenged with real human serum samples, showing consistent results when compared with a commercial standard assay kit. Such simple and easy-to-use approach may provide a new alternative for clinical and biological detection of ALP. Graphical abstract
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高影响因子
Biotechnology for Biofuels and Bioproducts
·
2022
·Vol. 15
·期 1
·1-12
·
IF 0
Liu Tian,Wang Peipei,Tian Jing,Guo Jiaqi,Zhu Wenyuan,Jin Yongcan,Xiao Huining,Song Junlong
Background Water-soluble lignin (particularly lignosulfonate, LS) has been well documented for its significance on enzymatic saccharification of lignocellulose, though the promotion mechanism has not been fully understood. Much attention has been paid to natural lignin or its derivatives. The disadvantage of using natural lignin-based polymers as promoting agents lies in the difficulty in tailor-incorporating functional groups due to their complex 3D structures. To further improve our understanding on the promotion mechanism of water-soluble lignin in the bioconversion of lignocellulose and to pursue better alternatives with different skeleton structures other than natural lignin, herein we reported a synthetic soluble linear aromatic polymer, sodium polystyrene sulfonate (PSS), to mimic LS for enhancing the efficiency of enzymatic saccharification. Results The role of PSS in enzymatic saccharification of pure cellulose and green liquor-pretreated poplar (GL-P) was explored by analyzing substrate enzymatic digestibility (SED) under different addition dosages and various pH media, along with LS for comparison. At the cellulase loading of 13.3 FPU/g-glucan, the glucose yield of GL-P increased from 53% for the control to 81.5% with PSS addition of 0.1 g/g-substrate. It outperformed LS with the addition of 0.2 g/g-substrate by 6.3%. In the pH range from 4.5 to 6, PSS showed a positive effect on lignocellulose saccharification with the optimum pH at 4.8, where the most pronounced SED of GL-P was achieved. The underlying mechanism was unveiled by measuring zeta potential and using Quartz Crystal Microbalance (QCM) and Multi-parametric Surface Plasmon Resonance (MP-SPR). The results confirmed that the complexes of cellulase and PSS were conjugated and the negatively supercharged complexes reduced non-productive binding effectively along with the improved saccharification efficiency. The thickness of PSS required to block the binding sites of cellulase film was less than half of that of LS, and the PSS adlayer on cellulase film is also more hydrated and with a much lower shear modulus than LS adlayer. Conclusions PSS as LS analogue is effective for enhancing the biomass enzymatic saccharification of GL-pretreated poplar. PSS exhibited a severer inhibition on the enzymatic saccharification of pure cellulose, while a more positive effect on bioconversion of lignocellulose (GL-P) than LS. In addition, a much lower dosage is required by PSS. The dynamic enzymatic hydrolysis indicated PSS could prolong the processive activity of cellulase. The valid data stemmed from QCM and SPR expressed that PSS bound to cellulases and the as-formed complexes reduced the non-productive adsorption of cellulase onto substrate lignin more efficiently than LS due to its flexible skeleton and highly hydrated structure. Therefore, PSS is a promising alternative promoting agent for lignocellulose saccharification. From another perspective, the synthetic lignin mimics with controllable structures enable us to reach an in-depth understanding of the promotion mechanism of soluble lignins on enzymatic saccharification.
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Advanced Composites and Hybrid Materials
·
2022
·Vol. 5
·期 2
·1003-1016
·
IF 11.806
Xie Wenhao,Yao Feichong,Gu Hongbo,Du Ai,Lei Qin,Naik Nithesh,Guo Zhanhu
Herein, a novel three-dimensional nanocomposite aerogel (rGO/Fe 3 O 4 /PANI NAs) with outstanding magnetoresistance and piezoresistance was manufactured by the in-situ polymerized polyaniline nanoarrays (PANI NAs) surrounding magnetic reduced graphene oxide (rGO/Fe 3 O 4 ) aerogel that was prepared through the combination of hydrothermal method and lyophilization method. This rGO/Fe 3 O 4 /PANI NAs nanocomposite aerogel with 60 wt.% loading of PANI NAs well preserved the porous structure and gained a superior mechanical strength (121.04 kPa) compared with that of rGO aerogel, rGO/Fe 3 O 4 aerogel, and rGO/PANI NAs aerogel (43.54, 58.12, and 116.98 kPa, respectively). The rGO/Fe 3 O 4 /PANI NAs nanocomposite aerogel could hold its original state with almost 100% recovery ratio after cycling compression tests under 80% of deformation strain at a suitable compression rate of 5 mm min −1 . The introduction of PANI NAs into the rGO/Fe 3 O 4 aerogel also brought a satisfactory piezoresistive performance with a large gauge factor up to 2.83 and a superb stability for the electrical signal output (which was decreased only 5.80% after 500 compression cycles) to the rGO/Fe 3 O 4 /PANI NAs nanocomposite aerogel. The loading of Fe 3 O 4 and PANI NAs also provided rGO/Fe 3 O 4 /PANI NAs nanocomposite aerogel with a negative magnetoresistance value up to − 4.37%. The magnetoresistance was explained via the amelioration of spin transport in the material. The unique negative magnetoresistance and excellent piezoresistance make rGO/Fe 3 O 4 /PANI NAs nanocomposite aerogel a promising candidate for the development of advanced electronic devices. Graphical abstract A novel three-dimensional nanocomposite aerogel (rGO/Fe 3 O 4 /PANI NAs) exhibits amazing negative magnetoresistance and outstanding piezoresistive performance.
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