SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Xilin Geng,Qionghua Xie,Yundong Zhu,Guocheng Yao,Liangshi Wang
Germanium is a strategic metal that is challenging to enrich and purify without any side effects, especially stability and selectivity. Here the role of carbonyl acid in selective separation of germanium from complex industrial liquids was highlighted. Integrating theoretical and experimental analyses, carbonyl acids exhibit strong binding ability and high selectivity to germanium was demonstrated. The first set of carbonyl acid extractant (CAE) for germanium extraction was successfully synthesized with a solubility of less than 50 mg/L at pH = 0. The process analysis exhibits the extraction rate of germanium in a single stage by CAEs exceeds 99.9 %, while the separation factor surpasses 10 3 , far beyond 10 1 of hydroxamic acids. Furthermore, the stripping rate for germanium in a single stage exceeds 83.0 %, with germanium accounting for over 99.9 % of the total metal content in the stripping solution. The CAE extraction process is straightforward and characterized by high germanium separation efficiency. The development of CAE not only realises the stable, green, low-carbon, and high-efficiency purification of germanium, but also the universality and inspiration of CAE make it highly significant and valuable in both theoretical research and practical applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Peng-Hao Zhao,Jun-Wu Cai,Yan Li,Qiao-Hui Li,Meng-Meng Niu,Xiang-Chen Meng,Fei Liu
This study reports the structure-activity relationships of a unique subclass IIb bacteriocin, plantaricin EvF, which consists of two peptide chains and possesses potent antimicrobial activity. Because the plantaricin Ev peptide chain lacks an α-helix structure, plantaricin EvF is unable to exert its antimicrobial activity through helix-helix interactions like typical subclass IIb bacteriocins. We have shown by various structural evaluation methods that plantaricin Ev can be stabilized by hydrogen bonding at amino acid residues R3, V12, and R13 to the N-terminal region of plantaricin F. This binding gives plantaricin EvF a special spade-shaped structure that exerts antimicrobial activity. In addition, the root-mean-square deviations (RMSDs) of the amino acid residues Y6, F8, and R13 of plantaricin Ev pre- and post-binding were 1.512, 1.723, and 1.369, respectively, indicating that they underwent large structural changes. The alanine scanning experiments demonstrated the important role of the above key amino acids in maintaining the structural integrity of plantaricin EvF. This study not only reveals the unique structural features of plantaricin EvF, but also provides an insight into the structure-activity relationships of subclass IIb bacteriocins.
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ACS Nano
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2024
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IF 15.8
Zhiying Wang,Ming Liu,Haocheng Lin,Guiying Zhu,Zaizai Dong,Nan Wu,Yubo Fan,Gaolian Xu,Lingqian Chang,Yang Wang
Strategies for rapid, effective nucleic acid processing hold tremendous significance to the clinical analysis of circulating tumor DNA (ctDNA), a family of important markers indicating tumorigenesis and metastasis. However, traditional techniques remain challenging to achieve efficient DNA enrichment, further bringing about complicated operation and limited detection sensitivity. Here, we developed an ion concentration polarization microplatform that enabled highly rapid, efficient enrichment and purification of ctDNA from a variety of clinical samples, including serum, urine, and feces. The platform demonstrated efficiently separating and enriching ctDNA within 30 s, with a 100-fold improvement over traditional methods. Integrating an on-chip isothermal amplification module, the platform further achieved 100-fold enhanced sensitivity in ctDNA detection, which significantly eliminated false-negative results in the serum or urine samples due to the low abundance of ctDNA. Such a simple-designed platform offers a user-friendly yet powerful diagnosis technique with a wide applicability, ranging from early tumor diagnosis to infection screening.
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TALANTA
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2024
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IF 5.6
Tong Zhang,Xiaojing Liang,Tiantian Si,Xiaofeng Lu,Shuai Wang
In response to the challenges associated with the chromatographic separation of polar compounds, this study aims to devise a solution by introducing a novel stationary phase. Hydrogels, characterized by a three-dimensional network structure, have aroused wide attention owing to its functional designability, multiple interaction sites and good adhesion, etc. In this work, an adhesive hydrogel functionalized silica stationary phase (Sil@PVA/TA) was synthesized using physical coating technique. Due to the co-existence of hydroxyl and benzene ring in the hydrogel structure, the obtained composites materials exhibited excellent separation performance for various of compounds and excellent column efficiency up to 71385.6 plates/m for thymidine. Furthermore, the hydrogel functionalized silica demonstrated superior selectivity to bare silica, diol-column and NH 2 -column for the separation of various of polar molecules, including, nucleosides/bases, alkaloids, organic acids, antibiotics and amino acids. Notably, for alkaloids, which frequently encounter peak tailing issues, Sil@PVA/TA demonstrated superior peak shape compared with C18 column. In short, this study successfully synthesized a hydrogel functionalized silica stationary phase, offering a novel method for the separation and analysis of polar compounds.
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Materials Today Chemistry
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2024
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IF 6.7
Peng He,Yanbin Zhao,Bin Wang,Yang Wang,Yangyang Li,Mei Li,Chenglin Chu,Bin Xu,Yu Cong
Magnesium phosphate cement as a substitute for osteoporotic fracture implants has gained increasing attention from the public. However, lack of sufficient mechanical strength and osteoinductive capacity, rapid setting time and strongly alkaline environment during degradation have hindered its practical application. Hence, a drug carrier system composed of chitosan and teriparatide solution of liquid phase is constructed and compounded into magnesium phosphate cement (CHI-TR MPC). The CHI-TR MPC composites exhibited almost neutral pH, extended setting time, excellent injectability , enhanced compressive strength , and increased porosity. Meanwhile，the CHI-TR MPC scaffold facilitated the controllable release of ions and drugs, promoted osteogenic differentiation and mineralization through its abundant pore structure , and possessed an appropriate degradation rate . Therefore, this CHI-TR MPC scaffold has huge potential for functional biomaterials design and clinical management in the treatment of osteoporotic fractures.
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BioChip Journal
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2024
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IF 5.5
Liu Guodong,Gong Zeyu,Dou Xiaolong,Li Chaojiang,Tan Qifeng,Liu Yuxuan,Zhang Xianchao,Qiu Yanzheng
Human sweat serves as a viable signal source for continuous monitoring of physiological indicators. Sweat sensors with flexible microfluidic structures offer the advantages of operational convenience and quantifiability, making them suitable for integration with electrochemical detection. The utilization of flexible polymer materials as substrates enhances the ability to manipulate sweat, necessitating exploration into highly integrated and controllable structural designs for fluid chambers in microfluidic structures. This study presents simulations and designs aimed at optimizing the layout of microfluidic structures and two-dimensional channel shapes. A flexible polyethylene glycol terephthalate substrate is employed to fabricate the microfluidic sensing device using laser cutting, screen printing, and layer-by-layer assembly techniques. The resulting microfluidic structure enables precise control over artificial sweat flow velocities and facilitates verification of Na + detection. The optimal parameters of the length of 7 mm, width of 110 μm, and diffusion angle of 15°, ensure stable flow velocity under precise control. The response of the Na + sensor exhibits near-Nernstian behavior that varies with the flow velocity. The integration of an electrochemical sensor with flexible microfluidic structures demonstrates high sensitivity and accuracy, and on-body quantitative analysis in human sweat is achieved.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Tao Wu,Jingqiu Zhou,Wanli He,Lei Jin,Tenghui Li,Tianxing Gong,Xu Liu
Chemotherapy is a standard preoperative treatment for locally advanced resectable gastric cancer (GC). However, its efficacy is often limited by the high intracellular glutathione (GSH) levels in tumor cells, which diminish the effectiveness of chemotherapeutic agents. In this study, we developed a GSH-sensitive injectable hydrogel, T-CMC@Fe 3+ (5-FU + GOx), incorporating ferric ions (Fe 3+ ), glucose oxidase (GOx), and 5-fluorouracil (5-FU) for advanced GC multimodal treatment. The hydrogel synergistically enhances anti-tumor activity through multiple mechanisms, including chemodynamic therapy, starvation therapy, and chemotherapy. Thiolated carboxymethyl cellulose (T-CMC) was synthesized by grafting L-cysteine onto carboxymethyl cellulose, with successful preparation confirmed by XPS, FTIR, and 13 C NMR characterizations. The T-CMC@Fe 3+ hydrogel demonstrated responsive degradation of GSH and exhibited favorable biocompatibility in co-culture experiments with GES-1 cells. In vitro analyses revealed that the T-CMC@Fe 3+ (5-FU + GOx) hydrogel significantly enhanced anti-tumor efficacy compared to chemotherapy alone, reducing cell viability to 12.28 ± 2.88 % after 48 h. In vivo studies using cell-derived and patient-derived xenograft models further confirmed its potent anti-tumor effects, with a marked reduction in tumor volume by 14 days (8.12 ± 4.89 mm 3 ). These findings highlight the potential of the T-CMC@Fe 3+ (5-FU + GOx) hydrogel as a novel and effective strategy for enhancing GC treatment through its multimodal therapeutic approach.
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IEEE SENSORS JOURNAL
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2024
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IF 4.3
Chaoli Tang,Dachao Fu,Rujing Wang,Xiaoyu Zhang,Liman Wei,Mingjun Li,Chengpan Li,Qiao Cao,Xiangyu Chen
On-site monitoring of phosphate concentration has attracted much attention recently for soil fertility management in agricultural products. This study proposes a portable and easy-operate electrochemical microfluidic system with integrated reagent reactions for on-site continuous monitoring of soil phosphate. Polydimethylsiloxane (PDMS) chips with channel layers and PDMS substrates embedded with screen-printed electrodes (SPEs) were prepared using 3-D printed molds. Reagents and samples were pumped into the chips at optimal flow rates of $100~\mu \text{L}$ min $^{-{1}}$ to automatically react, which were used for cyclic voltammetry (CV) electrochemical detection of analytes via carbon black nanoparticles (CBNPs) modified electrodes. After optimizing experimental conditions, including pH value, liquid flow rates, and voltammetry scan rates, the system achieved a linear phosphate concentration range of 5– $100~\mu \text{M}$ with a detection limit (LOD) of $4.2~\mu \text{M}$ ; moreover, the system exhibited excellent resistance to interference long lifespan and excellent phosphate recovery rate from 91.1% to 110.48%. This system has been successfully applied to continuous field phosphate determination in real soil environments. Results elucidated that the system has good practicality and great potential for fine nutrient management in the field.
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Advanced Healthcare Materials
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2024
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IF 10
Mengna Sun,Tianyu Shi,Subiyinuer Tuerhong,Mengru Li,Qiaoli Wang,Changchang Lu,Lu Zou,Qinghua Zheng,Yingxin Wang,Juan Du,Rutian Li,Baorui Liu,Fanyan Meng
In situ vaccination is an attractive type of cancer immunotherapy, and methods of persistently dispersing immune agonists throughout the entire tumor are crucial for maximizing their therapeutic efficacy. Based on the probiotics usually used for dietary supplements, an immunomodulator-boosted Lactococcus lactis (IBL) strategy is developed to enhance the effectiveness of in situ vaccination with the immunomodulators. The intratumoral delivery of OX40 agonist and resiquimod-modified Lactococcus lactis (OR@Lac) facilitates local retention and persistent dispersion of immunomodulators, and dramatically modulates the key components of anti-tumor immune response. This novel vaccine activated dendritic cells and cytotoxic T lymphocytes in the tumor and tumor-draining lymph nodes, and ultimately significantly inhibited tumor growth and prolonged the survival rate of tumor-bearing mice. The combination of OR@Lac and ibrutinib, a myeloid-derived suppressor cell inhibitor, significantly alleviated or even completely inhibited tumor growth in tumor-bearing mice. In conclusion, IBL is a promising in situ tumor vaccine approach for clinical application and provides an inspiration for the delivery of other drugs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yujie Xu,Congmin Niu,Shuyi Liang,Jiayi Guo,Kaiming Li,Jiarui Zhang,Jingyuan Li,Yaju Jin,Jingkun Bai,Jiangkun Dai,Chunbo Lu
The gut microbiota plays a significant role in the pathogenesis and remission of inflammatory bowel disease. However, conventional antibiotic therapies may alter microbial ecology and lead to dysbiosis of the gut microbiome, which greatly limits therapeutic efficacy. To address this challenge, novel nanomicelles that couple inulin with levofloxacin via disulfide bonds for the treatment of salmonellosis were developed in this study. Owing to their H 2 S-responsiveness, the nanomicelles can target the inflamed colon and rapidly release levofloxacin to selectively fight against enteric pathogens. Moreover, the embedded inulin can serve as prebiotic fiber to increase the amount of Bifidobacteria and Lactobacilli in mice with salmonellosis, thus maintaining the intestinal mechanical barrier and regulating the balance of the intestinal flora. Therefore, multifunctional nanomicelles had a better curative effect than pure levofloxacin on ameliorating inflammation in vivo . The pathogen-targeted glycovesicle represents a promising drug delivery platform to maximize the efficacy of antibacterial drugs for the treatment of inflammatory bowel disease.
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Advanced Healthcare Materials
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2024
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IF 10
Wei Zhang,Yijun Ran,Mi Yang,Yaqin Hu,Zhigang Wang,Yang Cao,Haitao Ran
Amplifying oxidative stress to disrupt intracellular redox homeostasis can accelerate tumor cell death. In this work, an oxidative stress amplifier (PP@T) is prepared for enhanced tumor oxidation therapy to reduce tumor growth and metastases. The nano-amplifier has been successfully constructed by embedding MTH1 inhibitor (TH588) in the PDA-coated porphyrin metal–organic framework PCN-224. The controllable-released TH588 is demonstrated from pores can hinder MTH1-mediated damage-repairing process by preventing the hydrolysis of 8-oxo-dG, thereby amplifying oxidative stress and exacerbating the oxidative DNA damage induced by the sonodynamic therapy of PP@T under ultrasound irradiation. Furthermore, PP@T can effectively induce immunogenic cell death to trigger systemic anti-tumor immune response. When administered in combination with immune checkpoint blockade, PP@T not only impedes the progression of the primary tumor but also achieves obvious antimetastasis in breast cancer murine models, including orthotopic and artificial whole-body metastasis models. Furthermore, the nanoplatform also provides photoacoustic imaging for in vivo treatment guidance. In conclusion, by amplifying oxidative stress and reactive oxygen species sensitized immunotherapy, this image-guided nanosystem shows potential for highly specific, effective combined therapy against tumor cells with negligible side-effects to normal cells which will provide a new insight for precise tumor treatment.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
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IF 15.7
Ren Zhihao,Shi Xiaoyu,Yang Qing,Li Chunsheng,Liu Hanqing,Bai Tiesheng,Ma Yuan,Das Pratteek,Liu Haofeng,Yang Endian,Jin Shengye,Feng Liang,Shi Quan,Bao Xinhe,Cheng Hui-Ming,Wu Zhong-Shuai
For electronics to be wearable, contactless charging and overall deformability are necessary pre-conditions. However, the current heterogeneous integration based on different active materials and separate manufacturing often leads to mechanical mismatch. Here, we report an ultrastretchable all-in-one integrated MXene-based microsystem comprising wireless coils, micro-supercapacitors (MSCs) and strain sensors. The seamless configuration without any connecting interface dramatically improves the structural integrity of the microsystem, and a pre-crumpled structure endows it with superior stretchability. Attributed to these, our MSCs can be wirelessly charged in ~20 s under various types of deformation and are capable of powering strain sensors, responding rapidly to body motion signals. Moreover, the MSCs display a high specific capacitance of 76.82 F cm –3 , and superb mechanical stability with 98.5% capacitance retention after biaxial stretching 1000 cycles from 0% to 500% areal strain. Therefore, this work sheds new insights into design and implementation of skin-attachable wireless microelectronics.
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