Frontiers in Bioscience-Landmark
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2025
·Vol. 30
·期 1
·26299
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IF 3.1
Zeliang Zhang,Wei Shang,Lisong Lin
Background:In this study, we prepared a porous gradient scaffold with hydroxyapatite microtubules (HAMT) and chitosan (CHS) and investigated osteogenesis induced by these scaffolds.Methods:The arrangement of wax balls in the mold can control the size and distribution of the pores of the scaffold, and form an interconnected gradient pore structure. The scaffolds were systematically evaluated in vitro and in vivo for biocompatibility, biological activity, and regulatory mechanisms.Results:The porosity of the four scaffolds was more than 80%. The 50% and 70% HAMT-CHS scaffolds formed an excellent gradient pore structure, with interconnected pores. Furthermore, the 70% HAMT-CHS scaffold showed better anti-compressive deformation ability. In vitro experiments indicated that the scaffolds had good biocompatibility, promoted the expression of osteogenesis-related genes and proteins, and activated the oxidative phosphorylation pathway to promote bone regeneration. Eight weeks after implanting the HAMT-CHS scaffold in rat skull defects, new bone formation was observed in vivo by micro-computed tomographic (CT) staining. The obtained data were statistically analyzed, and the p-value < 0.05 was statistically significant.Conclusion:HAMT-CHS scaffolds can accelerate osteogenesis in bone defects, potentially through the activation of the oxidative phosphorylation pathway. These results highlight the potential therapeutic application of HAMT-CHS scaffolds.
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Science China-Materials
·
2025
·
IF 7.4
Huang Lin,Wang Wantao,Liu Lei,Ma Wenzheng,Fan Jinghao,Zhou Dan,Zhao Lei,Zheng Zhaomin,Liu Hongmei,Wu Decheng
The treatment of intervertebral disc (IVD) degeneration remains a significant challenge due to the unique ischemic structure of the IVD, which comprises the scavenging of inflammatory cytokines, alleviation of cellular oxidative stress responses, restoration of nuclei pulposus (NP) cell viability, and recovery of IVD biomechanical function. Herein, we developed an injectable microsphere (CS-MnO 2 @PC) by incorporating chitosan microspheres (CS) with manganese dioxide (MnO 2 ) nanozymes and celecoxib encapsulated in Pluronic F-127 (PC) nanosized micelles, via in situ redox or Schiff base reaction. The hybrid carrier demonstrates robust capabilities in scavenging free radicals, alleviating extracellular oxidative stress, and reducing inflammatory cytokines in NP cells, as evidenced by RT-qPCR and immuno-fluorescence staining assays. In vivo evaluations further indicate that this hybrid carrier helps preserve NP hydration and the lamellar structure of the annulus fibrosus (AF), as confirmed by radiological analysis and histological staining evaluations. These injectable chitosan microspheres, combining nanozymes and nanosized drug micelles, represent a promising therapeutic strategy for degenerative IVD.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2025
·Vol. 36
·期 1
·1-11
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IF 4.5
Wang Yu-mei,Xin Tong,Deng Hao,Chen Jie,Tang Shen-lin,Liu Li-sheng,Chen Xiao-liang
Human hair keratin, a natural protein derived from human hair, has emerged prominently in the field of wound repair, showcasing its unique regenerative capabilities and extensive application potential. However, it is a challenge for the keratin to efficiently therapy the impaired wound healing, such as combined radiation-wound injury. Here, we report a keratin/chitosan (KRT/CS) film for skin repair of chronic wounds in in rats with combined radiation-wound injury. In brief, the KRT/CS film was characterized by scanning electron microscopy (SEM), mechanical property analysis, water absorption, and swelling analysis. A rat model of combined radiation-wound injury was employed to evaluate the therapeutic efficacy of the KRT/CS film. Finally, the systemic biotoxicity of KRT/CS film was assessed through histological analysis. The surface of KRT/CS film was uniform and smooth compared with the KRT film, and the mechanical property, swelling rate and water absorption rate of KRT/CS film were significantly improved, which can meet the application requirements of wound excipient dressing. Furthermore, the combined radiation-wound injury in rats was established that the wound closure rate was achieved 74.46% after 14 days of treatment with KRT/CS film, comparing to the single KRT membrane and commercially available Band-Aids. Histological analysis demonstrated that the amount of angiogenesis and collagen deposition in wounds treated with KRT/CS were significantly improved. These findings demonstrate the KRT/CS film as a promising therapeutic agent for combined radiation-wound injury. Graphical
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2024
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IF 4.5
Guohao Mu,Fang Peng,Ziyin Zhang,John M. Brameld,Peter Rose,Xiaogang Yang,Zhi-jiang Jiang,Xi He,Bencan Tang
This study designed and developed a novel three-layer electrostatic, slow-releasing nanoparticle drug delivery system for the treatment of ulcerative colitis. The nanoparticles were composed of layers of zein-based polydopamine, chitosan and cellulose acetate phthalate to form a polyelectrolyte multilayer core−shell nanoparticle structure. The anti-inflammatory natural product magnolol was chosen to be the model drug for the study. The nanoparticles (260.50 ± 23.90 nm) were prepared with layer-by-layer coating and then assessed in vitro and in vivo using a mouse model of ulcerative colitis. The in vitro data confirmed the slow-releasing and pH-dependent characteristics of particles. Quantitative analysis of the in vivo distribution of Mag in mice showed significantly higher concentrations in colonic tissue than in the upper gastrointestinal tract (854.6 ng/g vs 291.5–351.1 ng/g, respectively). Histological and pro-inflammatory cytokine analysis indicated that the nanoparticles had a significant anti-inflammatory effect and were protective against DSS-induced structural damage of the colonic mucosal structure. These results confirm that this nano-delivery system could increase the levels of Mag reaching the colon, resulting in greater efficacy than free Mag in treating UC in this mouse model.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Jian Zou,Xin Jing,Shitao Li,Yi Chen,Yuejun Liu,Pei-Yong Feng,Xiang-Fang Peng
Large mechanical hysteresis, stemming from the inherent viscoelasticity of the hydrogel networks, seriously affected its service life and application scope. Herein, we introduced a synergistic approach combining MXene nanoconfinement and bridging effect to produce hydrogels with low mechanical hysteresis. The introduced MXene was able to provide an effective nanoconfined effect on the polymerization of acrylamide monomers. By synergizing with the bridging effect—facilitated by strong interactions between chitosan-grafted polyacrylamide and solvent molecules to accelerate stress transfer—we successfully developed a MXene-reinforced conductive hydrogel with mechanical hysteresis as low as 3.17 %. Additionally, the strong electrostatic interactions between the chitosan and MXene further affiliate the dispersion of MXene within the hydrogel. The resulting MXene-reinforced conductive hydrogel demonstrated remarkable temperature sensitivity (TCR = −1.42 %/°C), making it suitable to be used as a health monitoring device. These findings opened up new perspectives for the further expansion of MXene and beyond.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yunchang Fan,Qiang Zhou,Sheli Zhang,Yanhe Nie
Thiosulfate has been considered as a more environmentally-friendly alternative to cyanide salts for the extraction of gold from gold ores and the development of affordable, green and efficient adsorbents for the isolation of gold-thiosulfate complex (Au(S 2 O 3 ) 2 3− ) from the leaching solution remains a significant challenge. To address this issue, chitosan, a natural macromolecule , was selected as a carrier and chemically modified with ionic liquids . The ionic liquids modified chitosan showed greater adsorption capacity towards Au(S 2 O 3 ) 2 3− compared with pristine chitosan. The adsorption of Au(S 2 O 3 ) 2 3− on ionic liquid modified chitosan followed Freundlich isotherm and pseudo-second order kinetic models, involving an anion-exchange mechanism with liquid film diffusion as the rate-limiting step. The chitosan modified with butylimidazolium-based ionic liquid had an adsorption capacity of 5.0 mg g −1 for gold (10 mg L −1 of gold, pH 6, 2 g L −1 of adsorbent dosage), outperforming other reported adsorbents. The ionic liquid modified chitosan showed a high adsorption efficiency of up to 96.7 % for Au(S 2 O 3 ) 2 3− in an actual thiosulfate leaching solution with a desorption efficiency of 98.4 %, suggesting that the ionic liquid modified chitosan has the potential to be a eco-friendly, biocompatible and effective adsorbent for the recovery of Au(S 2 O 3 ) 2 3− .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Ke Jian,Liming Fu,Yujie Zhang,Hongmei Zhang,Xiangjun Guo,Xihui Zhao
Yellow fluorescent carbon dots (Y-CDs) were prepared via microwave method using chitosan and o -phenylenediamine as the main raw materials. The obtained Y-CDs possesses good water solubility, excellent biocompatibility and luminous stability. During the microwave pyrolysis carbonization process, the surface of Y-CDs was modified with the functional groups such as amino and carboxyl, which can bind to Al 3+ by forming complexes, further improving the selectivity and sensitivity of the Al 3+ detection. And the fluorescence of Y-CDs was quenched by Al 3+ by static quenching process. More importantly, Y-CDs as fluorescent sensor was further applied for the determination of Al 3+ in the real water samples with high reliability and accuracy. In addition, Y-CDs present potential application in biological imaging. The cultivated zebrafish embryos with Y-CDs displayed clearly in vivo uptake and metabolic fluorescence images, further confirming its low toxicity and excellent biocompatibility.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Ting Liang,Cuntao Lu,Maofang Zhao,Xiaoying Cao,Jingwen Hao,Xinyue Zhang,Hailan Fu,Qinghe Cao,Ludan Li,Jihong Jiang
Wound infections are a growing public health issue, worsened by drug-resistant strains. Quercetin (Que) has shown anti-inflammatory, antioxidant, and antimicrobial properties, but its limited bioavailability hinders therapeutic use. This study introduces a multifunctional self-assembly nanoplatform, QHCNPs, encapsulating quercetin with hordein/chitosan to enhance stability and bioavailability. Transmission electron microscopy and particle size analysis revealed that QHCNPs are spherical structures with a diameter of 435.5 ± 2.9 nm and a Zeta potential of +11.0 mV. QHCNPs demonstrated excellent stability, low cytotoxicity, and MIC values of 512 ppm against Staphylococcus aureus ( S. aureus ) and 256 ppm against methicillin-resistant Staphylococcus aureus (MRSA) . In a bacterial wound model, QHCNPs outperformed quercetin alone by accelerating wound healing, eliminating bacteria, reducing inflammatory markers, scavenging reactive oxygen species (ROS), and promoting collagen and blood vessel regeneration. These results establish QHCNPs as a promising non-antibiotic therapy for treating drug-resistant wound infections, supporting further exploration for clinical applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaokang Na,Bowen Zou,Xiaohan Zheng,Xueer Yu,Ling Zhang,Xianbing Xu,Ming Du,Chao Wu
The persistent global issues of unsafe food and food waste continue to exist. Microbial contamination stands out as a major cause of losses in perishable foods like vegetables and fruits. Herein, we report a self-assembling coating based on disulfide bond cleavage-induced bovine serum albumin (BSA), where the antimicrobial activity of chitosan oligosaccharide (COS) is stably anchored in the coating by electrostatic interactions during the unfolding-aggregation phase of BSA. The intrinsic antimicrobial activity of COS, combined with the positively charged and hydrophobic regions enriched on the BSA coating, significantly disrupts the integrity of bacterial structures. Furthermore, the BSA@COS coating can easily adhere in situ to the grooves on the surface of strawberries through a simple one-step spraying process, extending the shelf life of strawberries and bananas by nearly three times. This makes it a potential economic alternative to current commercial antimicrobial coatings, offering a solution to the rampant global issue of food waste.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Luning He,Shuyi Xing,Weikang Zhang,Ying Wang,Yian Li,Jinghao Chen,Jinghai Zhang,Siling Wang,Qinfu Zhao
Traditional wound care methods are less effective for infectious and diabetic wounds, highlighting an urgent need for effective strategies. The study aimed to design a self-healing hydrogel with antibacterial, antioxidant, and photothermal capabilities to treat infectious and diabetic wounds. Silver nanoparticles (AgNPs) were loaded into mesoporous polydopamine (MPDA) nanoparticles to form Ag@MPDA nanoparticles. Ag@MPDA was incorporated into the cationic guar gum-chitosan-boric acid (CCB) hydrogel to obtain the PA-CCB hydrogel. PA-CCB hydrogel exhibited excellent self-healing and adhesive properties, adapting well to the dynamic wound environment. PA-CCB hydrogel combined with photothermal therapy (PTT) could effectively eradicated E. coli (99.9 %) and S. aureus (99.7 %). The PA-CCB hydrogel reduced excessive reactive oxygen species and promoted the migration of fibroblasts in vitro. In the infected mouse wound models, the PA-CCB hydrogel effectively inhibited bacteria. After combining with PTT, the antibacterial ability of the PA-CCB hydrogel was further enhanced. In the diabetic mouse wound models, the PA-CCB hydrogel reduced the inflammatory level of wound tissue. In both models, after combining with PTT, the PA-CCB hydrogel exhibited further improvements in angiogenesis, collagen deposition, and re-epithelialization. By integrating multifunctional hydrogel with PTT, the PA-CCB hydrogel exhibited broad application potential for infectious and diabetic wounds.
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Sustainable Energy & Fuels
·
2024
·
IF 5
Fuqiang Zhang,Zhiqiang Qi,Xiangsheng Han,Hongzhen Cai,Keyan Yang
Interfacial solar steam generation (ISSG) is an effective method to produce clean water through evaporating seawater actuated by solar energy. Nevertheless, developing a solar evaporator that is simultaneously simple in process and maintains good stability and high efficiency is still difficult but in great demand. Herein, an aerogel solar evaporator was prepared by cross-linking chitosan (CS) and two-dimensional transition metal carbide/nitride (MXene) nanosheets with excellent properties via a simple freeze-drying strategy. The unique three-dimensional network structure and good biocompatibility could facilitate quick transport of water from the bottom up to the evaporation surface by capillary force. MXene nanosheets combined a broad spectral response with strong solar absorption capacity, enabling the CS/MXene aerogel solar evaporator to exhibit strong light absorption, light-to-heat conversion, and water transport capabilities. The results showed that the water evaporation rate under one sun was as high as 1.80 kg m−2 h−1, with an energy conversion efficiency of 75.2%. Notably, the stability of the solar evaporator ensured stable solar water evaporation over a long period compared with most CS-based solar evaporators. Meanwhile, clean water could be continuously produced from acidic, alkaline and organic dye solutions, and saline brines. These tactics pave a new way for developing solar absorbers for solar-driven desalination.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhiying Shao,Xiaoyi Jiang,Qinlu Lin,Simiao Wu,Siming Zhao,Xinyang Sun,Yunhui Cheng,Yong Fang,Peng Li
The presence of potentially toxic elements and compounds poses threats to the quality and safety of fruit juices. Among these, Hg(II) is considered as one of the most poisonous heavy metals to human health. Traditional chitosan-based and selenide-based adsorbents face challenges such as poor adsorption capacity and inconvenient separation in juice applications. In this study, we prepared nano‑selenium functionalized chitosan gel beads (nanoSe@CBs) and illustrated the synergistic promotions between chitosan and nanoSe in removing Hg(II) from apple juice. The preparation conditions, adsorption behaviors, and adsorption mechanism of nanoSe@CBs were systematically investigated. The results revealed that the adsorption process was primarily controlled by chemical adsorption. At the 0.1 % dosage, the adsorbent exhibited high uptake, and the maximum adsorption capacity from the Langmuir isotherm model could reach 376.5 mg/g at room temperature. The adsorbent maintained high adsorption efficiency (> 90 %) across a wide range of Hg(II) concentrations (0.01 to 10 mg/L) and was unaffected by organic acids present in apple juice. Additionally, nanoSe@CBs showed negligible effects on the quality of apple juice. Overall, nanoSe@CBs open up possibilities to be used as a safe, low-cost and highly-efficient adsorbent for the removal of Hg(II) from juices and other liquid foods.
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