Journal of Integrative Medicine-JIM
·
2024
·
IF 4.2
Zhen Wang,Min Liu,Guang-xing Li,Liu Zhang,Kai-yue Ding,Si-qi Li,Bing-qing Gao,Peng Cheng,Hyok-Chol Choe,Lun-yue Xia,Yu-tong Yang,Yi Liu,Xue Sui,Jun-nan Ma,Lin Zhang
Objective Despite the combination of Scutellaria barbata D. Don and Scleromitrion diffusum (Willd.) R.J. Wang (SB-SD) being a recognized Chinese medicinal herbal pair that is commonly used in the treatment of ovarian cancer, there is a poor understanding of their pharmacological mechanisms. This study examines the antitumor properties and potential mechanisms of SB-SD on human ovarian cancer A2780 cells through a multi-omics approach, establishing a pharmacological basis for clinical utilization. Methods A range of mass ratios and reagents were used in the hot reflux extraction of SB-SD. The inhibitory effect of the SB-SD extracts on A2780 cell proliferation was assessed using the cell-counting kit 8 assay. A zebrafish tumor implantation model was used to evaluate the effects of SB-SD extracts on tumor growth and metastasis in vivo. Transcriptomics and proteomics were used to investigate alterations in biological pathways in A2780 cells after treatment with different concentrations of SB-SD extract. Cell cycle, cell apoptosis, intracellular free iron concentration, intracellular reactive oxygen species (ROS) concentration, malondialdehyde (MDA), and mitochondrial membrane potential were measured. Real-time quantitative reverse transcription polymerase chain reaction and Western blotting were utilized to investigate the effects of heme catabolism and ferritinophagy on ferroptosis induced by SB-SD extract in A2780 cells. Results The 70% ethanol extract of SB-SD (a mass ratio of 4:1) inhibited A2780 cell proliferation significantly with a half maximal inhibitory concentration of 660 μg/mL in a concentration- and time-dependent manner. Moreover, it effectively suppressed tumor growth and metastasis in a zebrafish tumor implantation model. SB-SD extract induced the accumulation of free iron, ROS, MDA, and mitochondrial damage in A2780 cells. The mechanisms might involve the upregulated expression of ferritinophagy-related genes microtubule-associated protein 1 light chain 3, autophagy-related gene 5, and nuclear receptor coactivator 4. Conclusions SB-SD extract effectively inhibited the development of ovarian cancer both in vitro and in vivo. Its mechanism of action involved inducing ferroptosis by facilitating heme catabolism and ferritinophagy. This herbal pair holds promise as a potential therapeutic option for ovarian cancer treatment and may be utilized in combination with routine treatment to improve the treatment outcomes of ovarian cancer patients.
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高影响因子
Chinese Journal of Natural Medicines
·
2017
·
IF 1.667
Qiong YU,Li-Long JIANG,Na LUO,Ya-Xi FAN,Jiang MA,Ping LI,Hui-Jun LI
Polygoni Multiflori Radix (PMR) has been commonly used as a tonic in China for centuries. However, PMR-associated hepatotoxicity is becoming a safety issue. In our previous in vivo study, an interaction between stilbenes and anthraquinones has been discovered and a hypothesis is proposed that the interaction between stilbene glucoside-enriching fraction and emodin may contribute to the side effects of PMR. To further support our previous in vivo results in rats, the present in vitro study was designed to evaluate the effects of 2, 3, 5, 4′-tetrahydroxystilbene-2- O-β -D-glucopyranoside (TSG) on the cellular absorption and human liver microsome metabolism of emodin. The obtained results indicated that the absorption of emodin in Caco-2 cells was enhanced and the metabolism of emodin in human liver microsomes was inhibited after TSG treatment. The effects of the transport inhibitors on the cellular emodin accumulation were also examined. Western blot assay suggested that the depressed metabolism of emodin could be attributed to the down-regulation of UDP-glucuronosyltransferases (UGTs) 1A8, 1A10, and 2B7. These findings definitively demonstrated the existence of interaction between TSG and emodin, which provide a basis for a better understanding of the underlying mechanism for PMR-induced liver injury.
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BIOCHEMICAL PHARMACOLOGY
·
2022
·
IF 6.1
Xiaodong Sun,Tengjiao Fan,Guohui Sun,Yue Zhou,Yaxin Huang,Na Zhang,Lijiao Zhao,Rugang Zhong,Yongzhen Peng
Chloroethylnitrosoureas (CENUs) exert antitumor activity via producing dG-dC interstrand crosslinks (ICLs). However, tumor resistance make it necessary to find novel strategies to improve the therapeutic effect of CENUs. 2-Deoxy-D-glucose (2-DG) is a well-known glycolytic inhibitor, which can reprogram tumor energy metabolism closely related to tumor resistance. Here, we investigated the chemosensitization effect of 2-DG on l,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) against glioblastoma cells and the underlying mechanisms. We found that 2-DG significantly increased the inhibitory effects of BCNU on tumor cells compared with BCNU alone, while 2-DG showed no obvious enhancing effect on the BCNU-induced cytotoxicity for normal HaCaT and HA1800 cells. Proliferation, migration and invasion determinations presented the same trend as survival on tumor cells. 2-DG plus BCNU increased the energy deficiency through a more effective inhibition of glycolytic pathway. Notably, the combination of 2-DG and BCNU aggravated oxidative stress in glioblastoma cells, along with a significant decrease in glutathione (GSH) levels, and an increase in intracellular reactive oxygen species (ROS). Subsequently, we demonstrated that the combination treatment led to increased apoptosis via activating mitochondria and endoplasmic reticulum stress (ERS) related apoptosis pathways. Finally, we found that the dG-dC level was significantly increased after 2-DG pretreatment compared to BCNU alone by HPLC-ESI-MS/MS analysis. Finally, in vivo , 2-DG plus BCNU significantly suppressed tumor growth with lower side effects compared with BCNU alone in tumor-bearing mice. In summary, we proposed that 2-DG may have potential to increase the sensitivity of glioblastoma cells to BCNU by regulating glycolysis, ROS and ERS pathways in clinical setting.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
Qimin Zhao,Jiayi Huang,Yongkang Yan,Danhua Ge,Tao Liu,Xiaojun Chen
Developing light-responsive nanozymes with integrated activity and stability holds significant importance for highly sensitive and accurate sensing in food safety and environmental protection. Herein, we developed a dual-mode (colorimetric and fluorescent) sensor based on an Fe-doped TiO2 nanosphere for d-penicillamine (D-PA) detection. The unique hollow structure of Fe-TiO2 provides a large surface area and abundant oxygen vacancies while facilitating rapid Fe(II)/Fe(III) cycling, collectively resulting in enhanced light-responsive peroxidase (POD)-like activity. Under light irradiation, the Fe-TiO2 promoted the generation of abundant reactive oxygen species, which oxidize TMB to blue oxTMB. Furthermore, the Fukui index and reaction kinetic trials indicated that the detection mechanism was primarily based on the antioxidant effect and redox activity of D-PA. The proposed sensor demonstrated detection limits (LODs) of 0.054 μM (colorimetric) and 0.40 μM (fluorescence), with 92.2–107.4% recovery and relative standard deviation < 4.4% in milk, tap water, and fetal bovine serum, along with outstanding reproducibility and stability. This work provides new insights to synthesize light-responsive nanozymes and integrated multimode sensing platforms.
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JOURNAL OF MICROENCAPSULATION
·
2019
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IF 2.04
Yingting Wang,Along Zuo,Xiaoyan Huang,Ying Ying,Xinsheng Shu,Xianxiong Chen,Yatao Yang,Junxian Ma,Guimiao Lin,Xiaomei Wang,Lin Mei,Gan Liu,Yingying Zhao
Taxane-based chemotherapy-loaded drug delivery systems have great potential for cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical cancer cell line Hela and the human breast cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and ‘self-controlled’ drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast cancer cells and human cervical cancer cells.
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高影响因子
BIOMEDICAL MICRODEVICES
·
2025
·Vol. 27
·期 4
·1-12
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IF 3.3
Zhang Jingfei,Nan Xuanxu,Yang Li,Cui Yue
The Lateral flow immunoassay (LFIA) has been widely used in environmental monitoring and disease diagnosis due to its advantages of low cost, simple operation, and convenience. However, its accuracy and sensitivity remain major challenges to be addressed. D-dimer is an important biomarker for thrombotic diseases. In this work, we show for the first time a core–shell Au@Ag nanoparticle (NP) labeled colorimetrically enhanced LFIA for D-dimer detection. The superior performance of Au@Ag LFIA stems from the silver shell's enhancement of plasmon resonance, which boosts optical signals to yield brighter scattering and superior visual contrast. Compared with conventional AuNPs, Au@AgNPs significantly improve sensitivity, leading to more accurate results. The detection limit for D-dimer was improved by approximately tenfold, reaching 1 ng/mL, due to the improved cross-coupling efficiency of Au@AgNPs compared with AuNPs. We anticipate that, with further development and validation, this enhanced LFIA could become a valuable tool in a wide range of clinical diagnostic applications.
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Engineering
·
2025
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IF 11.6
Chao Sun,Tianzuo Wang,Ruijie Gao,Xiaoyang Liu,Kang Xue,Chengxiang Shi,Xiangwen Zhang,Lun Pan,Ji-Jun Zou
Understanding the intrinsic features of dehydrogenation reactions of liquid organic hydrogen carriers (LOHCs) is a formidable challenge due to the combined impact of electronic and geometric effects. Herein, we constructed a series of Pt/MO x catalysts (CeO 2 , MgO, ZrO 2 , TiO 2 , Al 2 O 3 , or SiO 2 ) with similar sizes of Pt (∼1.7 nm) to investigate the effects of Pt electron structures (tuned by electronic metal‒support interactions) on the catalytic dehydrogenation of LOHCs. The results revealed a volcano-shaped correlation between Pt d electrons on different supports and the turnover frequency of catalytic dehydrogenation. Importantly, the Pt/MgO catalyst exhibited the highest dehydrogenation activity. With decreasing d electron content, Pt/MgO increases the bonding orbital dominance of Pt‒C bonds and leads to stable adsorption of H6-monobenzyltoluene (MBT), which facilitates subsequent C‒H bond scission. This study offers insight for the strategic development of high-efficiency dehydrogenation catalysts via d electron density modulation of Pt sites.
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高影响因子
ACS Omega
·
2019
·
IF 2.584
Zifeng Zheng,Zhixiang Cui,Junhui Si,Shengrui Yu,Qianting Wang,Wenzhe Chen,Lih-Sheng Turng
Composite tissue engineering scaffolds show good comprehensive properties, but improving their interfacial binding force is still a challenge. In this study, polydopamine (PDA) was used as a glue-like layer to solve the interface problem in three-dimensional (3-D) porous hydroxyapatite (HA)/thermoplastic polyurethane (TPU) composite scaffolds and to improve their comprehensive properties. The morphology, water absorption, porosity, hydrophilicity, mechanical properties, and cell viability of HA/TPU and PDA/HA/TPU scaffolds were systematically studied. The scanning electron microscopy and X-ray photoelectron spectroscopy results showed that a uniform PDA coating layer successfully formed on the surface of 3-D porous HA/TPU composite scaffolds by dopamine self-polymerization. Moreover, the conjugation effect occurred between the PDA and HA/TPU matrix, resulting in an increase in the interfacial binding force of the 3-D PDA/HA/TPU scaffold. The hydrophilicity of the PDA/HA/TPU scaffolds was significantly higher than those of the TPU scaffolds. Both static and dynamic mechanical property tests revealed that the PDA/HA/TPU scaffold possessed excellent comprehensive mechanical properties, such as high strength, high stiffness, and good flexibility, due to the enhancement of the interfacial binding force. Compared with TPU and HA/TPU scaffolds, the addition of a PDA coating could effectively improve the attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) incubated on PDA/HA/TPU scaffolds. These results indicate that the PDA/HA/TPU scaffolds possess great potential to be used as tissue engineering scaffolds, and that the PDA coating could be a simple, practical, effective, and universal approach to modify composite scaffolds.
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Biology-Basel
·
2026
·Vol. 15
·期 18
·1652
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IF 4.3
Haoyu Chai,Jutao Li,Cuiluan Ma,Yu-Cai He
A sequential two-stage chemo-biocatalytic process was investigated for the conversion ofD-xylose to furfuryl alcohol (FOL), an important furan derivative widely used in the manufacture of resins, polymers, fuels, and other value-added products. In the first stage,D-xylose was dehydrated to furfural (FAL) in a ternary Betaine:Formic acid:Malonic acid–water (Bet:FA:MA–H2O) reaction medium. Under the selected conditions, a FAL yield of 65.8% was obtained from 22.5 g/LD-xylose using 15 wt% Bet:FA:MA at 170 °C for 30 min. For the subsequent bioreduction stage, a recombinantEscherichia colistrain co-expressing the aldehyde reductase EutG and glucose dehydrogenase (GDH) was constructed. Using glucose as a co-substrate, EutG–GDH whole cells were able to reduce commercial FAL at concentrations up to 150 mM under the selected conditions of 40 °C and pH 7.5. For the sequential process using theD-xylose-derived dehydration liquor, the acidic reaction mixture was adjusted to pH 7.5 and diluted from 98.7 to 30.0 mM FAL before whole-cell bioreduction. The conditioned 30.0 mM FAL feed was essentially completely converted to FOL within 24 h. These results demonstrate the laboratory-scale feasibility of sequentially combining Bet:FA:MA–H2O-mediatedD-xylose dehydration with EutG–GDH whole-cell FAL reduction after interstage pH adjustment and dilution.
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高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Xiaoqiong Li,Yunruo Xu,Luming Xu,Qilin Li,Yiwei Zhao,Chunyu Wei,Yuyu Li,Lin Wang,Zheng Wang
Surgery is the mainstay of treatment for patients with solid tumors. However, the intricate inflammatory and immunosuppressive tumor microenvironment after surgery is recognized as a primary cause of tumor recurrence and metastasis. Here, we design and construct an in situ injectable therapeutic hydrogel encapsulating 1-methyl-d-tryptophan (1-d-MT), a small-molecule competitive inhibitor of indoleamine 2,3-dioxygenase, and meloxicam, a selective inhibitor of cyclooxygenase-2 (Ge1MT/Mel hydrogel) to prevent postoperative tumor metastasis and relapse. In this hydrogel system, 1-d-MT increases cytotoxic T lymphocytes and decreases regulatory T cells, thereby reversing the tumor immune tolerance microenvironment, while released meloxicam reduces inflammation caused by postoperative trauma via the COX-2/PGE2 pathway. When delivered into the resected tumor cavity, the hydrogel system mimics a “hot” tumor-immunity niche to attack residual tumor cells and significantly suppresses the postoperative recurrence of colorectal cancer. In the model of postoperative breast cancer and colorectal cancer recurrence and metastasis, the Ge1MT/Mel hydrogel substantially reduces the volume of recurrent tumors and demonstrates 85.1% and 66.7% inhibition rates of metastases, respectively. Importantly, the Ge1MT/Mel hydrogel markedly stimulates a potent antitumor immune response in the cancer lesion microenvironment, increasing antitumor immune cells including CD8+ T cells, as well as elevating antitumor cytokines such as interferon-γ. Overall, combination immunotherapy via the Ge1MT/Mel hydrogel after surgery represents a promising strategy to minimize residual tumor burden and reduce recurrence risk following tumor resection.
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高影响因子
POLYMER
·
2026
·
IF 5.1
Chenying Zha,Xiaoyi Liu,Cheng Zhang,Zeshuang Qiao,Hongwei Bai
Stereocomplex-type polylactide (SC-PLA) composed of alternatingly packed enantiomeric chains has recently been regarded as a promising engineering bioplastic with favorable physical properties. However, it is challenging to realize its commercial applications since homocrystallization is fiercely competed with the SC crystallization in the melt-processing of enantiomeric poly( l -lactide) (PLLA) and poly( d -lactide) (PDLA) blends holding relatively high molecular weights (>100 kg/mol). Herein, a facile yet efficient strategy is proposed to facilitate the SC crystallization by using zinc acetate (Zn(OAc) 2 ) to strengthen intermolecular interactions. It is impressive to find that the addition of only 0.01 wt% Zn(OAc) 2 induces the selective formation of SC crystals (SCs) without any homocrystals (HCs), under various melt-crystallization conditions, exhibiting an extremely high efficiency in enhancing SC crystallizability. Moreover, the crystallization kinetic is significantly enhanced. Such enhancement could be ascribed to the metal-oxygen coordination between Zn 2+ ions from Zn(OAc) 2 and carbonyl group of PLAs, which favors the interactive collaboration between enantiomeric chains and thus lowers the barrier to the formation of SCs. This work provides valuable guidance for developing advanced SC-PLA materials with desirable crystallizability.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2026
·
IF 3
Xiaolong Ma,Shujun Chen,Anqin Li,Wuying Chen,Mingze Hou,Kun Long,Gang Hu,Biao Qin
To address the insufficient activity, low noble metal utilization efficiency, and poor stability of transition metal-based catalysts in alkaline media, a PtFeCoNiMnMo high-entropy alloy (HEA) nanocatalyst with synergistic multi-d orbital hybridization was synthesized via a one-pot solvothermal method using glucose as a dispersant and an auxiliary reducing agent. Comprehensive characterization (XRD, TEM, XPS, and ICP-OES) confirmed its single face-centered cubic structure with lattice distortion (δ = 3.29%), homogeneous elemental distribution, and directional electron transfer-induced strong electronic interactions. Electrochemical tests in 1 M KOH showed exceptional alkaline hydrogen evolution reaction (HER) performance: 21 mV overpotential at 10 mA cm−2, 47.06 mV dec−1 Tafel slope, 2.455 s−1 TOF, and minimal charge-transfer resistance (5.39 Ω), outperforming control samples and commercial Pt/C. The catalyst exhibited outstanding stability with only 2.1% current decay after 50 h. Mechanistic studies indicated that the multi-d orbital electronic interactions in the alloy can regulate the adsorption capacities of H and OH species, thereby enhancing its alkaline HER catalytic activity.
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