NUCLEIC ACIDS RESEARCH
·
2024
·
IF 16.6
Wang Xu,Qin Geng,Yang Jie,Zhao Chuanqi,Ren Jinsong,Qu Xiaogang
G-quadruplexes (G4s), as an important type of non-canonical nucleic acid structure, have received much attention because of their regulations of various biological processes in cells. Identifying G4s-protein interactions is essential for understanding G4s-related biology. However, current strategies for exploring G4 binding proteins (G4BPs) include pull-down assays in cell lysates or photoaffinity labeling, which are lack of sufficient spatial specificity at the subcellular level. Herein, we develop a subcellular selective APEX2-based proximity labeling strategy to investigate the interactome of mitochondrial DNA (mtDNA) G4s in living cells. By this method, we have identified several mtDNA G4BPs. Among them, a previously unrecognized mtDNA G4BP, DHX30 has been selected as an example to explore its important biofunctions. DHX30 localizes both in cytoplasm and mitochondria and can resolve mtDNA G4s. Further studies have demonstrated that DHX30 unfolds mtDNA G4 in living cells, which results in a decrease in glycolysis activity of tumor cells. Besides, RHPS4, a known mtDNA G4 stabilizer, will reverse this inhibition effect. Benefiting from the high spatiotemporal resolution and the ability of genetically encoded systems to perform the labeling with exquisite specificity within living cells, our approach can realize the identification of subcellular localized G4BPs. Our work provides a novel strategy to map protein interactions of specific nucleic acid features in subcellular compartments of living cells.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Wanling Cui,Yaxian Shao,Mengying Niu,Enguang Lv,Rui Wang,Mei Qiao,Xiaoxin Wang,Zhaohong Qian
Sensitive detection of antibiotic residues in food is of great significance for food safety monitoring. Herein, a novel nucleic acid amplification sensing platform for protein enzyme-free, label-free, and homogeneous fluorescence detection of kanamycin had been established through combining catalytic hairpin assembly (CHA) circuit and double-end G-quadruplex signal amplification. Here, kanamycin initiated the CHA circuit between two assembled probes by specifically binding to aptamer sequence. Each of the two assembled probes encapsulated the G-rich sequence in the stem region, so kanamycin-catalyzed CHA circuit produced the active double-end G-quadruplex assemblies under the action of K+. The double-end G-quadruplex assemblies further were combined with N-methylmesoporphyrin IX to induce a distinctly amplified fluorescence signal. The CHA-based double-end G-quadruplex signal amplification significantly improved the detection sensitivity of kanamycin. The fluorescence signal increment results confirmed that the result of double-end G-quadruplex assemblies was more than twice higher than those of single-end G-quadruplex assemblies. The system exhibited a wide linear range of 1 nM to 500 nM for kanamycin, and the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.27 nM and 0.89 nM respectively. In addition, the sensing system effectively distinguished kanamycin from other antibiotics, exhibiting high selectivity for kanamycin. The method also successfully detected spiked kanamycin in cow milk samples, and the recoveries of spiked kanamycin were in range of 99.00%―100.23% with RSDs in range of 1.71%―2.63%. All results verified the application potential of the system in the field of food detection.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Xiaorong Gan,Leilei Ye,Huimin Zhao,Dangyuan Lei,Yanhui Ao,Dan Zhao,Peifang Wang
Microenvironments in heterogeneous catalysis have been recognized as equally important as the types and amounts of active sites for regulating catalytic activity. Two-dimensional (2D) nanospaces between van der Waals (vdW) gaps of layered materials provide an ideal microenvironment to create novel functionalities. Here, we explore a facile method for fabricating g-C3N4/2H-MoS2 superlattice-like heterostructures based on thermochemical intercalation and polymerization reactions of formamide within enlarged vdW gaps of 2H-MoS2 nanosheets without any transfer process. DFT calculations demonstrate that the interlayer electron–electron correlations due to the intercalation effect of g-C3N4, rather than high-κ dielectric environments, lead to the improvement of intrinsic conductivity of 2H-MoS2 nanosheets. As the proof of concept in applications for the electrocatalysis field, the heterostructure for hydrogen electrochemical reaction (HER) exhibits high stability and catalytic activity in both acid and alkaline media, such as a quite low onset overpotential of 98 mV, a high exchange current density of 77.6 μA cm–2, and a small Tafel slope (52.9 mV dec–1) in an acid medium. The enhanced HER activity is attributed to the improved conductivity and nanoconfinement effect of 2D nanospaces that decrease the reaction activation energy and activate the inert basal planes.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yangyang Dai,Tian Fang,Shumin Li,Yuanyuan Wang,Suyue Zhong,Wenjun Su,Jian Li
While mixed matrix membranes (MMMs) containing two-dimensional (2D) layered materials (such as, GO, g-C 3 N 4 and MXene , etc.) have revealed potential application prospects for gas separation applications, it still faces a serious challenge to enhance the performance of CO 2 separation. Owing to the structural defects of g-C 3 N 4 nanosheets produced during the exfoliation process, which has greatly reduced the gas selectivity. Herein, we describe the construction of g-C 3 N 4 -MXene/Pebax MMM composed by MXene modified the structural defects in g-C 3 N 4 nanosheets to surmount the low gas selectivity of g-C 3 N 4 membrane. However, the stacking of nanosheets on each other hinder the gas transport channels. The TiO 2 nanoparticles derived in-situ from MXene semi-oxidation can support an intermediate layer between g-C 3 N 4 nanosheet and MXene nanosheet to expand the gas transport channels. Eventually, a novel g-C 3 N 4 -TiO 2 @MXene/Pebax MMM was obtained that exhibited maximum CO 2 permeability of 1673.69 Barrer in separation of CO 2 /CH 4 , CO 2 /N 2 selectivity of 45.13, and CO 2 /CH 4 selectivity of 47.76, exceeding the bond 2008 limit on Robeson. What's more, the MMM within 100 h (1 bar and 298 K) are measured to have outstanding separation performance. Overall, this concept offers a novel strategy to enhance CO 2 separation by MXene modified defects in g-C 3 N 4 nanosheets.
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ChemSusChem
·
2024
·
IF 7.5
Dong Li,Kaixuan Wang,Ying Chen,Renyi Li,Jiahong Tang,Yizhou Zhao,Wei Guo,Qi Chen,Yujing Li
Graphical A 0D/1D CPB@TCN heterojunction photocatalyst for CO 2 reduction is synthesized by immobilizing CsPbBr 3 (CPB) quantum dots on one-dimensional porous tubular g-C 3 N 4 (TCN). The strong Pb−N chemical bonding between CPB quantum dots and TCN provides a fast charge transfer channel for the CPB@TCN, which promotes the efficient separation and transfer of photogenerated carriers, resulting in excellent photocatalytic activity. Halide perovskite CsPbBr 3 (CPB) quantum dots (QDs) are considered as a promising candidate for solar-driven CO 2 conversion for their unique optoelectronic properties and suitable band structures. However, the CO 2 conversion efficiency of pristine CsPbBr 3 quantum dots is severely limited due to their rapid electron-hole pair recombination and insufficient active sites. In this study, by immobilizing CPB QDs onto one-dimensional (1D) porous tubular g-C 3 N 4 (TCN), an effective 0D/1D CPB@TCN heterojunction photocatalyst for CO 2 reduction is fabricated. Density functional theory (DFT) calculations combined with experimental studies demonstrate that CPB QDs are uniformly anchored on the surface of TCN through Pb−N chemical bonding, resulting in efficient separation and transfer of photogenerated carriers in CPB@TCN photocatalysts. The resultant CPB@TCN photocatalysts exhibit significantly enhanced photocatalytic activity for CO 2 reduction with the highest conversion rate of 22.62 μmol g −1 h −1 , which is 5.33-times higher than that of pristine CPB QDs. This work unveils the interfacial bonding mechanism of CPB@TCN heterojunction through Pb−N chemical bond, which provides new insights for the development perovskite-based heterojunction photocatalysts.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Guanhan Chen,Wenhui Ding,Wenyi Dong,Hongjie Wang,Shuting Zhu,Tianzhe Liang,Cheng Luo,Yuxiong Huang
The wide occurrence of pharmaceutically active contaminants (PhACs) in aquatic environments has received more and more concern owing to the associated high risks to ecosystems. Even worse, conventional wastewater treatment techniques fail to provide effective decontamination for these emerging contaminants . Herein, we designed and constructed mAg 3 PO 4 @g-C 3 N 4 heterojunction for a persulfate-assisted photocatalytic system (mAg 3 PO 4 @g-C 3 N 4 /PDS/Vis) for the rapid and effective degradation of PhACs. Superior mineralization of CBZ (87.15 %) was achieved within 30-minute treatment with mAg 3 PO 4 @g-C 3 N 4 /PDS/Vis, exhibiting 11-fold enhancement compared to mAg 3 PO 4 /Vis system. Integrating experimental investigations with theoretical calculations, singlet oxygen ( 1 O 2 ) was identified as the key reactive species, while the 1 O 2 -dominated ring-opening pathway contributed significantly to the high mineralization of CBZ. Moreover, mAg 3 PO 4 @g-C 3 N 4 /PDS/Vis showed robust degradation performance of multiple PhACs across various water matrices. Multiple PhACs could be simultaneously degraded with high mineralization efficiency in the wastewater effluent, demonstrating the promising application of mAg 3 PO 4 @g-C 3 N 4 /PDS/Vis for decontaminating emerging contaminants in wastewater treatment .
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Yunqi Ning,Daqi Lv,Qi Tang,Hanbing Wang,Xiaowan Hu,Yuming Cao,Shansheng Yu,Hongwei Tian
Photocatalysts for hydrogen evolution reaction (HER) have widely been explored but fine-tuning and engineering multiphase photocatalyst components are still challenging. Among catalysts, ZnIn 2 S 4 has shown promise but maximizing the use of ZnIn 2 S 4 -photogenerated carriers and enhancing quantum yield still require further investigation. One way to achieve this is by enhancing the photon utilization and reducing the recombination of shallow carriers in ZnIn 2 S 4 . Accordingly, novel ZnIn 2 S 4 /g-C 3 N 4 /MoS 2 type-II heterojunction composites with 2D/2D/2D structures (abbreviated ZIS/CN/MS) were synthesized in the present work by simple thermal condensation-solvothermal method. The optimized ZIS/CN/MS exhibited a photocatalytic hydrogen evolution rate of 13.6 mmol g −1 h −1 under visible light (λ ≥ 420 nm) irradiation, a value much higher than those of pure-phases ZIS and CN, as well as the binary composite of ZIS/CN. The apparent quantum efficiency of the optimized ZIS/CN/MS at a wavelength of 420 nm reached 27.0 % coupled with excellent cycling stability. The significantly improved photocatalytic performance and the excellent stability can mainly be attributed to the unique 2D/2D/2D arrangement of the ternary components of ZIS, CN, and MS in ZIS/CN/MS photocatalyst, providing a large number of close interfacial contacts between the components for shortened migration distance of photogenerated charges and more pathways for electron separation and transport in type-II heterostructures. Overall, the proposed methodology and ZIS/CN/MS composites with type-II heterojunction and 2D/2D/2D structures have great potential for use in solar-to-hydrogen energy conversion and can be extended to the preparation of other photocatalysts.
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MATERIALS CHEMISTRY AND PHYSICS
·
2024
·
IF 4.3
Shizheng Zhang,Hongliang Xu,Qianqian Qin,Chaoning Huang,Yongqiang Niu,Mingliang Li,Bo Song,Bingbing Fan,Gang Shao,Hongxia Lu,Hailong Wang
Vermiculite/g-C 3 N 4 /BiOBr composite (MCVB-X, X = 20, 50, 70, 90) with significantly improved photocatalytic performance was prepared by in-situ growing 20 wt%, 50 wt%, 70 wt%, 90 wt% of BiOBr nanosheets , respectively, on the surface of vermiculite/g-C 3 N 4 composite (MVC). With the increasing of BiOBr content, the photocatalytic performance of MCVB-X composites enhanced gradually. After 180 min of photocatalytic reaction under the visible light irradiation , the degradation rate of tetracycline (TC) by MCVB-50, which contained 50 wt% of BiOBr, reached 91.34 %, which was close to that of pure BiOBr and much higher than those of MVC and bulk g-C 3 N 4 (CN). The reaction rate constant (k) (0.0131 min −1 ) of MVCB-90 was 38.53 times and 6.24 times that of CN and MVC, respectively, which was also greater than that of pure BiOBr nanosheets (0.0098 min −1 ). The main reason for the significant improvement of photocatalytic performance of MCVB-X composites was that the construction of g-C 3 N 4 /BiOBr heterojunction promoted the separation and migration of photogenerated carriers and reduced the recombination rate of photogenerated electron-hole pairs. The agglomeration of BiOBr nanosheets was inhibited and contributed to the adsorption of pollutants. Moreover, the band structure , main active species (⋅O 2 − ) and photocatalytic degradation mechanism were proposed.
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RSC Advances
·
2025
·Vol. 15
·期 2
·1311-1322
·
IF 4.6
Wenzhe Li,Xudong Wang,Ye Chen,Pengcheng Wu,Zhengtao Li,Zhiqiang Wang,Deng Gu
Loading with non-metal cocatalysts to regulate interfacial charge transfer and separation has become a prominent focus in current research. In this study, g-C3N4/CNT composites loaded with non-metallic cocatalysts were prepared through in situ pyrolysis using urea and CNTs. Various characterization techniques, including transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS), photoelectrochemical (PEC) analysis, fluorescence lifetime spectroscopy (TRPL), electron paramagnetic resonance spectroscopy (ESR), and photoluminescence (PL) spectroscopy, were employed to analyze the sample's microstructure, phase composition, elemental chemical states, and photoelectronic properties. The photocatalytic performance of the g-C3N4/CNT composite in the degradation of actual drilling hydraulic filtrate was evaluated under simulated sunlight irradiation, with the degradation results assessed through COD and chroma measurements. The findings indicate that in g-C3N4/CNT composites loaded with non-metal cocatalysts, the photocatalytic performance improves significantly as the CNT content increases. At a CNT mass fraction of 6.0%, the composite achieves optimal performance, with a COD degradation rate of 0.3742 h−1 and a chroma degradation rate of 81%. These results offer valuable insights into enhancing photocatalytic treatment of waste drilling hydraulic filtrate using g-C3N4-based non-metallic cocatalysts.
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Science China-Materials
·
2024
·Vol. 67
·期 7
·2142-2152
·
IF 6.8
Li Yaying,Yang Huijuan,Li Jili,Li Yefei,Ren Wei,Wen Jin,Xiao Qi,Xu Jingsan
Heterojunction photocatalysis has been widely studied as a means of efficiently converting solar energy to chemicals. However, the major challenge in developing high-performing heterojunction photocatalytic systems lies in achieving efficient transfer of electrons between the components. Herein, a novel S-scheme heterojunction photocatalyst was developed by combining BaTi 2 O5 nanorods with g-C 3 N 4 lamellae. The preferential deposition of Pt nanoparticles as cocatalyst via the one-step impregnation-reduction method on g-C 3 N 4 nanosheets with enhanced interfacial contact and strong electronic interaction has been proved essential for the photocatalytic performance. The developed Pti mp /20BaTi 2 O 5 /g-C 3 N 4 photocatalyst delivers the optimal hydrogen production rate of 2587 µmol g −1 h −1 with high stability after cycles. Photoelectrochemical analysis and theoretical calculation suggest that the formation of BaTi 2 O 5 /g-C 3 N 4 heterojunction results in the staggered band alignment and improved charge carrier dynamics. This work highlights the importance and feasibility of promoting photocatalysis by a new S-scheme heterojunction with viable cocatalysts.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Lingyi Zhang,Jiachun Wu,Hongyun Xu,Huixia Li,Xiang Liu,Yanhua Song,Yanjuan Cui
The fabrication of non-metallic semiconductor heterojunction with superior redox capability for hydrogen (H 2 ) evolution from water and environmental remediation has been emerging as a prospective strategy. Herein, a novel two-dimensional (2D) g-C 3 N 4 /Ni-MOF Z-scheme heterojunction was prepared by a facile sonication-gel self-assembly method with g-C 3 N 4 and nickel metal-organic framework (Ni-MOF) nanosheets . The ultra-thin nanosheet structure of Ni-MOF was conducive to the formation of stable 2D heterojunctions. The opposite surface charge and matched band difference caused the charge flow from g-C 3 N 4 to Ni-MOF, resulting in an interfacial built-in electric field. The optimized NMF/CN-9 attained the optimal 3aphotocatalytic activity towards the degradation of tetracycline (TC) and H 2 evolution from water. Under visible light irradiation , the reaction rate for TC degradation (0.00497 min −1 ) and H 2 evolution (15.6 μmol·h −1 ) over NMF/CN-9 was nearly 2.4 and 2.1 folds higher than that of g-C 3 N 4 , respectively. Besides, the photocatalytic performance of NMF/CN-9 was also nearly 2 times higher than that of g-C 3 N 4 under simulated solar illumination. Such improvements were originated from higher photo-excited charge separation and superior redox ability derived from Z-scheme interfacial charge transfer. A possible photocatalytic mechanism was also proposed and the results indicated that efficient photo-induced electrons and reactive hole (h + ), superoxide radical (·O 2 - ) and hydroxyl radical (·OH) played a major role during the photocatalytic route. This work offers an intense insight into the construction of non-metallic semiconductor 2D heterojunctions for H 2 evolution and environmental wastewater treatment .
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FUEL
·
2024
·
IF 6.7
Boxiao Zhao,Jun Xu,Xiaoming Liu,Along Gao,Huiqin Guo,Liushui Yan,Jun Lin
Various of CoS 2 /g-C 3 N 4 nanosheet composites were fabricated through supramolecular self-assembly and copolymerization approaches. This fabrication entailed the incorporation of CoS 2 nanoparticles into ultra-thin layers of g-C 3 N 4 nanosheets. Demonstrating a significant boost, these composites achieved a 28-fold increase in the photocatalytic H 2 evolution from H 2 O with visible light response, without the aid of noble metal nanocrystalline co-catalysts. The enhanced CoS 2 /g-C 3 N 4 composite sustained approximately 92.4 % of its initial photocatalytic activity after four repeated uses. The planar structure of g-C 3 N 4 nanosheet amplifies its specific surface area, which improves access to catalytic sites and reduces the transport distance for photo-excited electrons and holes. Moreover, the close conjunction of CoS 2 nanoparticles and g-C 3 N 4 nanosheets establishes p-n heterojunctions, which promote swift charge separation and transfer at the material interface. Based on these characteristics, an initial model for the photocatalytic process has been proposed. With its remarkable photocatalytic performance and durability, the developed CoS 2 /g-C 3 N 4 composite presents substantial potential for solar-driven H 2 production.
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