MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Zhihao Wu,Tao Shi,Qi Shao,Dongmei Chen,Peisheng Gao,Jie Wang,Ting Xu,Qingqing Meng,Shaoshun Li
Early detection and precise treatment for breast cancer are crucial, given its high global incidence rate. Hence, the development of novel imaging targets is essential for diagnosing and monitoring resistance to chemotherapy, which is pivotal for achieving precise and personalized treatment for breast cancer patients. In our previous work, we successfully developed a near-infrared (NIR) probe 1 for CYP1B1-targeted imaging. In this study, we aimed to investigate the utility of the probe as a NIR fluorescence and photoacoustic dual-mode imaging probe for the detection and surveillance of breast cancer. Western blotting of cancer cell lines has confirmed that CYP1B1 is widely expressed in breast cancer and gynecological cancer. In vitro NIR fluorescence imaging capability of the probe for tracking CYP1B1-positive tumor cells was validated by using confocal microscopy. Further studies, including in vivo fluorescence and photoacoustic dual-model imaging and ex vivo biological distribution analysis on a triple-negative breast cancer xenograft mouse model, demonstrated that the probe selectively accumulated in tumor tissue within as early as 0.5 h postinjection. The results of the surgical resection experiment revealed that the tumor could be entirely removed under the guidance of NIR imaging, thereby indicating the probe’s efficacy in surgical navigation. CYP1B1 expression was found to be upregulated in adriamycin (ADR)-resistant breast cancer cells, MCF-7/ADR. Consequently, the sensitivity of CYP1B1 overexpressed cells, MCF-7/1B1, to ADR was significantly reduced, with an IC50 value of 0.586 ± 0.0934 μM, compared to the parental MCF-7 cells with an IC50 value of 0.183 ± 0.0444 μM. In vivo and ex vivo imaging assays conducted on MCF-7/ADR tumor-bearing mice demonstrated that the probe was specifically enriched in tumor sites, suggesting its potential for monitoring chemotherapy resistance in breast cancer. This study expands the scope of application for NIR probe 1, establishing its utility in breast cancer diagnosis through fluorescence-photoacoustic dual-model imaging, monitoring of chemotherapy resistance, and guidance for surgical resection. This strategy paves the way for novel approaches to precise and personalized treatment for breast cancer patients.
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高影响因子
ANALYTICAL CHEMISTRY
·
2016
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IF 5.886
Yanfen Wei,Dan Cheng,Tianbing Ren,Yinhui Li,Zebing Zeng,Lin Yuan
The real-time monitoring of key biospecies in the living systems has received thrusting attention during the past decades. Specifically, fluorescent detection based on near-infrared (NIR) fluorescent probes is highly favorable for live cells, live tissues, and even animal imaging, owing to the substantial merits of the NIR window, such as minimal phototoxicity, deep penetration into tissues, and low autofluorescence background. Nevertheless, developing potent NIR fluorescent probes still poses serious challenges to the chemists because traditional NIR fluorophores are less tunable than visible-wavelength fluorophores. To address this issue, here we report a set of novel NIR hybrid fluorophores, namely, the hybrid chromenylium-cyanine fluorophore (CC-Fluor), in which both the fluorescence intensity and the emission wavelength can be easily adjusted by the conformational changes and substitution groups. Compared to known NIR fluorophores, the new CC-Fluors are substantially advantageous for NIR probe development: (1) CC-Fluors display tunable and moderate Stokes shifts and quantum yields; (2) the fluorophores are stable at physiological conditions after long-term incubation; (3) the absorption maxima of CC-Fluors coincide with the common laser spectral lines in mainstream in vivo imaging systems; (4) most importantly, CC-Fluors can be easily modified to prepare NIR probes targeting various biospecies. To fully demonstrate the practical utility of CC-Fluors, we report two innovative NIR probes, a ratiometric pH probe and a turn-on Hg2+ probe, both are successfully employed in live animal imaging. Hence, the detailed studies allow us to confirm that CC-Fluors can work as an excellent platform for developing NIR probes for the detection of species in living systems.
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高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
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IF 7.328
Haofei Huang,Dengqi He,Xiaozhu Liao,Huajing Zeng,Zengjie Fan
The lacks of antibacterial properties, low adhesion and delayed wound healing of the hydrogel wound dressings limit their applications in wound treatment. To resolve these, a novel hydrogel composed of polydopamine (PDA), Ag and graphene oxide (GO) is fabricated for wound dressing via the chemical crosslinking of N-isopropylacrylamide (NIPAM) and N , N ′-methylene bisacrylamide (BIS). The prepared hydrogel containing PDA@Ag5GO1 (Ag5GO1 denotes the mass ratio between Ag and GO is 5:1) exhibits effective antibacterial properties and high inhibition rate against E. coli and S. aureus . It shows high adhesion ability to various substrate materials, implying a simpler method to the wound obtained by self-fixing rather than suturing. More important, it can produce strong contractility under the irradiation of near-infrared light (NIR), exerting a centripetal force that helps accelerate wound healing. Thus, the hydrogel containing a high concentration PDA@Ag5GO1 irradiated by NIR can completely repair the wound defect (1.0 × 1.0 cm 2 ) within 15 days, the wound healing rate can reach 100%, which was far higher than other groups. Taken together, the new hydrogel with excellent antibacterial, high adhesion and strong contractility will subvert the traditional treatment methods on wound defect, extending its new application range in wound dressing.
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高影响因子
Journal of Innovative Optical Health Sciences
·
2024
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IF 2.3
Yuxiang Gao,Chi Zhang,Lijun Zhu,Zhong Du,Rong Ma,Le Guo,Nuernisha Alifu,Xueliang Zhang
Triple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early stages often lacks noticeable symptoms, making it challenging to detect. Near-infrared II (NIR-II) fluorescence microscopic imaging obtains long wavelength, which enables reduced scattering, high spatial resolution and minimal autofluorescence, it is also a favorable imaging method for tumor diagnosis. PbS@CdS quantum dots (QDs) are one of the popular NIR-II fluorescence nanoprobes for well brightness. In this study, NIR-II emissive PbS@CdS QDs were utilized and further encapsulated with thiol-terminated poly(ethylene oxide) (SH-PEG, MW = 5000) to form PbS@CdS@PEG QDs nanoparticles (NPs). The obtained PbS@CdS@PEG QDs NPs were then characterized and further studied in detail. The PbS@CdS@PEG QDs NPs had large absorption spectra, exhibited strong NIR-II fluorescence emission at approximately 1300 nm, and possessed good NIR-II fluorescence properties. Then, the mice model of early-stage brain metastases of TNBC was established, and the PbS@CdS@PEG QDs NPs were injected into the tumor-bearing mice for NIR-II fluorescence microscopic bioimaging. The brain vessels and tumors of the living mice were detected with high spatial resolution under the NIR-II fluorescence microscopic imaging system with irradiation of 808 nm laser. The tumor tissues were further restricted and prepared as thin slices. The NIR-II fluorescence signals were collected from the tumor slices with high spatial resolution and signal-to-background ratio (SBR). Thus, the PbS@CdS@PEG QDs NPs-assisted NIR-II fluorescence microscopic system can effectively achieve targeting brain metastases of TNBC imaging, offering a novel and promising approach for TNBC-specific diagnosis.
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高影响因子
DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 29
·10965-10972
·
IF 4.569
Jianbang Zhou,Pengyun Chen,Junhao Li,Mingming Yang,Wei Jiang,Qiuhong Zhang,Haiyong Ni,Jiansheng Huo
Cr3+-Activated broadband near-infrared (NIR) luminescence materials are attracting much attention as next-generation smart NIR light sources that are widely used in night vision, bioimaging, medical treatment, and many other fields. Herein, a series of Na3GaxAl1−xF6:Cr3+ NIR phosphors with broadband emission and tunable luminescence properties were designed and prepared. The luminescence intensity, peak position and full width at half maximum (FWHM) of the materials can be controlled by adjusting the crystal field strength. Furthermore, Na3Ga0.75Al0.25F6:0.35Cr3+ exhibited high luminous efficiency and the emission intensity remained 81% at 423 K compared with the initial value at 298 K. The structural confinement and the electron–phonon coupling (EPC) effect may account for its good thermal stability. Finally, a pc-NIR-LED device with a photoelectric conversion efficiency of 6.53% at 350 mA was fabricated by combining the as-prepared NIR phosphor and a blue InGaN chip, and its applications in night vision and medical fields were further investigated. This work will promote the development of NIR phosphors with tunable luminescence properties.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Shengxin Li,Ziyan Zhang,Zhiran Zhang,Sen Zhou,Mengkai Liu,Xichao Li,Zheng Zheng,Jie Sun
The moisture, oil, protein, and nervonic acid contents are crucial criteria for assessing the nutritional value and processing quality of Xanthoceras sorbifolia Bunge seed kernels (XSKs). Near-infrared spectroscopy (NIRS) offers a viable alternative to conventional chemical methods for rapid and non-destructive sample analysis. This study employed a highly adaptive method known as competitive adaptive reweighted sampling (CARS), which facilitates variable selection for different components and streamlines the feature-variable screening process. Using this method, various algorithms were applied to establish NIRS detection models for the four components of XSKs, and accuracy evaluations were conducted using the prediction set determination coefficient ( R P 2 ), root-mean-square error (RMSEP), and other parameters. The results indicated that CARS effectively extracted key information on various components in NIRS. Based on the feature variables selected by CARS, the partial least squares regression (PLSR) model exhibited optimal performance regarding moisture ( R P 2 = 0.978, RMSEP = 0.117 %) and nervonic acid content ( R P 2 = 0.988, RMSEP = 0.072 %). Meanwhile, the particle swarm optimization-enhanced support vector regression (PSO-SVR) model and back-propagation neural networks (BPNN) achieved superior performance for protein ( R P 2 = 0.989, RMSEP = 0.243 %) and oil content ( R P 2 = 0.978, RMSEP = 0.467 %), respectively. These models enable rapid and non-destructive detection of multi-component content, thereby facilitating the expeditious identification and selection of XSKs with high nutritional value and superior quality.
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高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Xiuna Jia,Mengmeng Lv,Yunwei Fei,Qing Dong,Hao Wang,Qiong Liu,Dan Li,Jin Wang,Erkang Wang
Diagnosis-guided synergistic treatment based on innovative nanomaterials is of great significance for the development of anti-cancer therapies. However, the low delivery efficiency of therapeutic gene and the inability to trigger release on demand are still major obstacles impeding its wide application. Herein, we report an ultra-fast one-step method within 2 min to prepare a smart carrier, liposome-coated Prussian blue @ gold nano-flower, which is named LPAR after linking with tumor-targeting peptide. The versatile LPAR not only can respond to near-infrared (NIR) light, achieve the selective delivery and the controlled release of siRNA targeting the mutant gene of Kras at its codon-12 from Glycine (G) to Aspartic acid (D) (named as G12D mutant gene) in the malignant pancreatic tumors, but also efficiently convert the absorbed NIR light into the heat to realize gene-photothermal synergistic therapy both in vitro and in vivo . Theoretical simulation results reveal that the outstanding photothermal conversion efficiency of LPAR is mainly due to its higher electric field intensity and power density distributions . Furthermore, the LPAR possesses the capabilities for triple-modal imaging. Therefore, the developed NIR light-responsive LPAR has the potential to be served as a tumor-targeted nano-delivery system for imaging-guided synergistic therapy of cancers.
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高影响因子
Small
·
2025
·
IF 12.1
Wei Ge,Beibei Gao,Shuxian Li,Guangbo Yu,Lu Gao,Zhemin Shen,Fu Wang,Junle Qu
Ratiometric fluorescence imaging in the second near-infrared (NIR-II) region holds great promise for the accurate identification of metastatic lymph nodes (MLNs), but faces challenges in biocompatible probe design with simplified composition and broad spectral tunability. Here, a minimalist, self-calibrating ratiometric probe based on homologous Ag 2 Se quantum dots (QDs) is developed. Through rational redesign of tributylphosphine-mediated coordination kinetics, continuous size-dependent emission tuning across 935–1710 nm, with the synthetic mechanism and structure–property relationships systematically elucidated, is achieved. The inherently low-toxicity Ag 2 Se core, combined with poly(amino acid) coating, ensures excellent biosafety, as validated by in vitro cytotoxicity and in vivo biochemical assays. Two spectrally distinct QDs (emitting at 1040 and 1305 nm, with full width at half maximum of 176 and 119 nm) enable multiplexed anatomical imaging, allowing both real-time intraoperative detection and postoperative assessment of MLNs, with a diagnostic threshold ratio of ≈0.9. This work presents a scalable strategy for full-NIR-II monochromatic QD synthesis and establishes a minimal-component biocompatible platform for precision ratiometric diagnostics.
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高影响因子
TETRAHEDRON
·
2026
·
IF 2.1
Fan Wang,Zhiyu Wang,Kun Yu,Yuqing Wang,Qi Su,Chunxv Han,Wenxuan Hu,Lei Hu,Ping Li,Hui Wang
Abnormal mitochondrial viscosity is closely associated with tumor initiation and progression. Therefore, the development of fluorescent probes capable of real-time, precise monitoring of mitochondrial viscosity changes in tumor cells holds significant potential for the early diagnosis and therapeutic evaluation of tumors. This study designed and synthesized a series of near-infrared (NIR) fluorescent probes ( W1–W3 ) with D-π-A structural framework based on a phenothiazine-bithiophene-pyridinium moiety. All three probes ( W1 – W3 ) featured emission at approximately 650 nm, accompanied by a substantial Stokes shift of about 150 nm. Among them, W2 and W3 exhibited excellent responsiveness to viscosity. Their molecular rotor character results in quenched fluorescence in low-viscosity environments and intensified emission in high-viscosity media, enabling highly sensitive detection of viscosity changes. Cellular experiments revealed that W2 possesses outstanding biocompatibility and precise mitochondria-targeting ability. Remarkably, it achieved effective bioimaging at a concentration as low as 500 nM. The probe also succeeded in dynamically monitoring viscosity changes during drug-induced (e.g., DXM, rotenone) mitochondrial dysfunction. Notably, W2 detected increased mitochondrial viscosity in tumor cells triggered by apoptosis inducers (e.g., DOX, cisplatin), thereby confirming its capability to track dynamic changes in mitochondrial viscosity. In vivo and in vitro imaging results established the potential of probe W2 for tumor imaging. With its capability for dynamic viscosity monitoring from cellular to in vivo levels, probe W2 positions itself as a promising tool to advance applications in early tumor diagnosis, drug efficacy assessment, and precision therapy.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Ting Cao,Mengjin Li,Hong Ma,Zhefeng Fan
Monoamine oxidase (MAOs) is a key enzyme that maintains amine homeostasis and participates in liver pathological processes. Real time in situ monitoring of its activity is crucial for elucidating the mechanism of drug-induced liver injury (DILI). This study successfully constructed a ratio type fluorescent probe Cy-1 with mitochondrial targeting ability and near-infrared emission characteristics. This probe is based on MAOs catalyzed oxidative deamination reaction, and achieves fluorescence response through a clever “ICT (Intramolecular Charge Transfer) + ESIPT (Excited-State Intramolecular Proton Transfer)” dual mechanism, producing significant ratiometric signal changes in the near-infrared region. Experiments have shown that Cy-1 has a wide linear range (10–115 μg/mL), high sensitivity (0.86 μg/mL), and high selectivity in vitro, and can specifically localize to mitochondria. The probe was successfully used for in situ differential monitoring of endogenous MAOs activity in living cells, and visualized semi quantitative monitoring of MAOs activity changes in vivo was achieved in an acetaminophen (APAP) induced drug-induced liver injury (DILI) model. Cy-1 is expected to become a powerful chemical tool for understanding the mechanisms of MAOs in physiological and pathological processes, especially in the occurrence and development of diseases such as liver injury, as well as evaluating the efficacy of related drugs.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Zhen Wan,Qian Wu,Tian Deng
Periodontitis is a chronic inflammatory disease initiated by bacterial pathogens, resulting from complex interactions between microbial infection and host immune responses. In current clinical practice, antibiotics represent the most frequently utilized adjunctive therapy for periodontitis. However, the overuse of antibiotics not only contributes to the emergence of bacterial resistance but also demonstrates limited efficacy in inflammation resolution. In this study, we synthesized selenium/Ti 3 C 2 (Se/Ti 3 C 2 ) through a one-step redox method using Ti 3 C 2 as a template. The Se/Ti 3 C 2 demonstrated significant antibacterial activity against Escherichia coli ( E.coli ), Staphylococcus aureus ( S.aureus ), and Porphyromonas gingivalis ( P.gingivalis ) under near-infrared (NIR) light irradiation, as evidenced by comprehensive evaluations including colony-forming unit (CFU) counting assays, live-dead fluorescence staining, scanning electronic microscopy (SEM) observations, growth curves, and membrane potential staining assays. Furthermore, the anti-inflammatory and antioxidant properties of Se/Ti 3 C 2 were investigated using various methodologies, including 1,1-diphenyl-2-pyridine-hydratedacyl (DPPH) scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) scavenging assay, glutathione peroxidase (GPX) activity measurement, intracellular reactive oxygen species (ROS) fluorescence staining, flow cytometry, and quantitative real-time polymerase chain reaction (qPCR) assays. Through GPX1 gene knockdown experiments, we demonstrated that the anti-inflammatory effects of Se/Ti 3 C 2 were closely associated with GPX1 expression. RNA sequencing analysis was subsequently performed to elucidate the underlying mechanisms of its anti-inflammatory and antioxidant activities. Finally, in vivo experiments using a mouse periodontitis model demonstrated the remarkable therapeutic potential of Se/Ti 3 C 2 in periodontitis treatment. Therefore, Se/Ti 3 C 2 holds great promise as a potential alternative to antibiotics, emerging as a novel therapeutic material for periodontitis treatment.
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高影响因子
CANCER LETTERS
·
2026
·
IF 11.8
Lin-Ling Lin,Jing-Wen Bai,Liang Song,Kang-Liang Lou,Yi-Yang Gao,Hong-Yu Chen,Sheng-Jie Lin,Cheng-Xi Li,Yun Zhang,Guo-Jun Zhang
The precise intraoperative delineation of tumor margins and the controlled intratumoral release of chemotherapeutic agents remain major challenges in breast cancer therapy. Tumor microenvironment (TME)-responsive nanoplatforms provide a promising approach by utilizing the distinctive biochemical properties of tumors to achieve targeted imaging and treatment. Herein, we developed a glutathione (GSH)-activatable theranostic nanoprobe (DSM-cRGD) emitting fluorescence in the second near-infrared window (NIR-II). The probe is constructed from Nd-doped rare-earth nanoparticles coated with a manganese dioxide (MnO 2 ) shell and functionalized with cyclic RGD peptide for α v β 3 -integrin targeting. The MnO 2 shell quenches NIR-II fluorescence during systemic circulation and undergoes GSH-responsive degradation within the TME. In addition, the nanoprobe shows strong targetability within tumors via specific binding to α v β 3, thereby enabling real-time, high-contrast surgical navigation. Moreover, a newly developed therapeutic agent, DSM-cRGD@DOX, formed by loading doxorubicin (DOX) onto DSM-cRGD, enables the simultaneous release of DOX and Mn 2+ ions upon degradation of the MnO 2 shell in the tumor microenvironment (TME), thereby inducing synergistic chemodynamic and chemotherapeutic effects. This further induces immunogenic cell death, activating anti-tumor immune responses and suppressing tumor recurrence and metastasis. Collectively, this DSM-cRGD-based platform represents an integrated nanoplatform that combines precise tumor imaging with stimuli-responsive drug delivery, offering an advanced strategy for the precision management of breast cancer.
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高影响因子
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