JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 15
·3054-3062
·
IF 3.614
Qin Lei,Abduh Murshed,Anthony Chukwunonso Ogbuehi,Qian Peng,Yiqing Zhang,Fenyong Sun,Qi Zhong,Lei Jin,Hao Wang
Phosphorylation is one of the most important protein post-translational modifications, which possesses dramatic regulatory effects on the function of proteins. In consideration of the low abundance and low stoichiometry of phosphorylation and non-specific signal suppression, efficient capture of the phosphoproteins from complex biological samples is critical to meet the need for protein profiling. In this work, a facile preparation of titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles was developed for the enrichment of intact phosphoproteins. The prepared magnetic nanoparticles were characterized by various instruments and had a spherical shape with an average diameter of 300 nm. The adsorption isotherms were investigated and the maximum capacity for β-casein was calculated to be 961.5 mg/g. Standard protein mixtures and biological samples (non-fat milk and human serum) were selected to test the enrichment performance. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated the excellent enrichment performance with high selectivity. With the superparamagnetic property, titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles were convenient for the practical application and clinical promotion, thus having a promising prospect in the field of phosphoprotein research.
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PubMed
高影响因子
MATERIALS CHARACTERIZATION
·
2025
·
IF 5.5
Xianshu Dong,Tianshun Dong,Jingkun Li,Yanfei Jiang,Binguo Fu,Guolu Li
To improve the wear resistance of Al 30Si coatings, nano-Y₂O₃-reinforced Al 30Si (Y₂O₃/Al-30Si) coatings were fabricated on 6063 aluminum alloy substrates by atmospheric plasma spraying (APS). The effect of nano-Y 2 O 3 addition on the coating was studied in detail. The results show that nano-Y₂O₃ reduced the porosity of the Al 30Si coatings from 8.2 % to 3.1 %. Nano-Y₂O₃ can provide heterogeneous nucleation sites for primary silicon (β-Si) phases, enabling the Y₂O₃/Al-30Si coating to achieve a 57.4 % reduction in β-Si phase size. Correspondingly, the microhardness, elastic modulus and fracture toughness of the coating increased by 11.0 %, 11.2 % and 48.9 %, respectively. Under the present test conditions, the Y₂O₃/Al-30Si coating exhibited a lower coefficient of friction and a 27.2 % reduction in wear volume. Both the Y₂O₃/Al-30Si and Al 30Si coatings exhibited abrasive, oxidative, and fatigue wear mechanisms.
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高影响因子
JOURNAL OF RARE EARTHS
·
2025
·
IF 7.2
Xiao-Yu Guo,Jun-Hao Wang,Ting-Zhou Li,Kai Xu,Chun-Jiang Jia,Hao Dong,Ding Ma,Chen Li,Ya-Wen Zhang
The efficient ammonia decomposition catalysts are crucial for advancing the application of hydrogen energy produced from ammonia. Herein, La 2 Ti 2 O 7 and LaTiO 2 N are developed as supports for constructing efficient Ru/La 2 Ti 2 O 7 and Ru/LaTiO 2 N ammonia decomposition catalysts. Compared with monoxide supported catalysts such as Ru/La 2 O 3 , Ru/TiO 2 and Ru/SiO 2 , Ru/La 2 Ti 2 O 7 shows higher activity, achieving an NH 3 conversion rate of 66.5% at 450 °C with a fairly low Ru loading amount (about 1.4 wt%). Using Ru/LaTiO 2 N, we achieve an even higher NH 3 conversion rate (76.1%), hydrogen production rate (1556 mmol/(g Ru ·min)), and 140-hour catalytic stability at 450 °C with a GHSV of 30000 mL/(g cat ·h). Various characterizations demonstrate that the presence of Ti n + is conductive to the adsorption of NH 3 , while the presence of La 3+ with its coordinated O 2– endows La 2 Ti 2 O 7 and LaTiO 2 N with robust surface basicity facilitating the recombination and desorption of N*, together assisting the active metal Ru to catalyze ammonia decomposition. This study provides new material options and design insights from the collective contribution of multiple atom sites in a complex multicomponent heterogeneous catalytic system for the development of efficient Ru-based ammonia decomposition catalysts.
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高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2015
·
IF 2.59
Hua Sun,Zhiguo Ma,Danyi Lu,Baojian Wu
ABSTRACT In th is study, we aimed to determine the glucuronidation potential of psoralidin in humans and to perform validation on use of psoralidin-3- O -glucuronidation as afunctional marker for UGT1A9. Glucuronidation kinetics was determined using human liver microsomes (HLMs), human intestine microsomes (HIM), and expressed UDP-glucuronosyltransferase (UGT) enzymes. The chemical structures of metabolites were determined by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analyses. Validation of psoralidin-3- O -glucuronidation as a UGT1A9 marker was performed using combined approaches including reaction phenotyping, chemical inhibition, activity correlation analysis, and determination of relative activity factor (RAF). HLM and UGT1A9 generated two monoglucuronides (9- O -glucuronide and 3- O -glucuronide) from psoralidin, whereas HIM, UGT1A1, UGT1A7, and UGT1A8 generated one only (9- O -glucuronide). Formation of 3- O -glucuronide in HLM was markedly inhibited by the UGT1A9-selective inhibitors magnolol and niflumic acid . Further, psoralidin-3- O -glucuronidation was strongly correlated with propofol-glucuronidation in a group of nine individual HLMs ( r = 0.978, p < 0.001). Strong correlation was also observed between psoralidin-3- O -glucuronidation and the UGT1A9 protein levels measured by Western blotting ( r = 0.944, p < 0.001). Moreover, UGT1A9 was responsible for 99.6% of psoralidin-3- O -glucuronidation in HLM based on the RAF approach. In conclusion, psoralidin was subjected to efficient glucuronidation, generating one or two monoglucuronides depending on UGT isozymes . Also, psoralidin-3- O -glucuronidation was an excellent in vitro marker for UGT1A9.
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2019
·
IF 3.926
Du-Chao Zhou,Yu-Ting Lu,Yan-Wen Mai,Chen Zhang,Jie Xia,Pei-Fen Yao,Hong-Gen Wang,Shi-Liang Huang,Zhi-Shu Huang
For the development of novel anticancer agents, we designed and synthesized a total of 37 perimidine o -quinone derivatives containing the o -quinone group at the A or B ring and different substituents (alkyl groups, aryl groups or heterocycles) at the C ring of the compounds. The structure-activity relationships (SARs) were established based on the cytotoxicity data of compounds from the HL-60, Huh7, Hct116, and Hela cell lines. The cytotoxicity results showed that most compounds exhibited potent cytotoxicity. In particular, compound b-12 showed the best anti-proliferative activity (IC 50 ≤ 1 μM) against four cancer cell lines and strong potency against the HL-60/MX2 (0.47 μM) cell line, which is resistant to Topo II poisons. Further studies showed that b-12 exhibited potent Topo IIα inhibitory activity (IC 50 = 7.54 μM) compared with Topo I, which acted as a class of non-intercalative Topo IIα catalytic inhibitor by inhibiting the ATP binding site of Topo II. Cell apoptosis and cell cycle assays confirmed that b-12 could induce the apoptosis of Huh7 cells in a dose-dependent manner.
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PubMed
高影响因子
Environmental Science-Nano
·
2022
·Vol. 9
·期 7
·2572-2586
·
IF 9.473
Wen Chen,Xiang Ye,Qiong Tang,Ting Yu,Pengcheng Tu,Xiaodong Zheng
Nano-plastic pollution has been increasingly recognized as a key environmental issue. Nano-plastic exposure is linked to a multitude of environmental and health effects, and we have previously reported that cyanidin-3-O-glucoside (C3G) promoted discharge and reduced the biotoxicity of polystyrene (PS). This study further determines the impact of C3G on PS exposure, and investigates the role of energy metabolism in the ameliorative effects of C3G. Through a Caenorhabditis elegans (C. elegans) model, C3G was discovered to be capable of promoting discharge and alleviating biotoxicity induced by environment-relevant PS exposure. C3G was also found to restore PS-induced ATP reduction accompanied by improved mitochondrial dysfunction, suggesting involvement of energy metabolism. Using a Caco-2 cell model, we found that the molecular mechanism involves C3G protecting against PS-induced ATP reduction by activating the AMPK/SIRT1/PGC-1α signaling pathway. C3G-mediated ATP production and mitochondrial functions, which improve the activity of ABC transporters and cellular efflux of PS, may constitute the primary toxicity-alleviating mechanism through which C3G acts. Furthermore, in a mouse model administered with PS, it was discovered that C3G activates the AMPK/SIRT1/PGC-1α signaling pathway regulating the ATP level in colon tissue, in addition to the increased fecal PS discharge. These findings suggest that C3G protects against adverse effects of PS exposure on multiple organism models, and the underlying mechanism involves mitochondria-related energy metabolism and efflux.
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高影响因子
Food & Function
·
2021
·Vol. 12
·期 18
·8351-8365
·
IF 5.396
Yonglu Li,Xin Yu,Yaxuan Wang,Xiaodong Zheng,Qiang Chu
Lung cancer has been threatening human health worldwide for a long time. However, the clinic therapies remain unsatisfactory. In this study, the anti-adenocarcinoma lung cancer A549 cell line abilities of Tetrastigma hemsleyanum tuber flavonoids (THTF) were evaluated in vivo, and isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis was conducted to detect the protein alterations in THTF-treated solid tumors. The differentially expressed proteins were related to the cytoskeleton and mostly accumulated in the calcium signaling pathway. The in vitro study illustrated that 80 μg mL−1 THTF significantly suppressed cellular viability to approximately 75% of the control. Further results suggested that kaempferol-3-O-rutinoside (K3R), the major component of THTF, effectively triggered cytoskeleton collapse, mitochondrial dysfunction and consequent calcium overload to achieve apoptosis, which remained consistent with proteomic results. This study uncovers a new mechanism for THTF anti-tumor ability, and suggests THTF and K3R as promising anti-cancer agents, providing new ideas and possible strategies for future anti-lung cancer prevention and therapy.
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PubMed
高影响因子
INORGANIC CHEMISTRY
·
2020
·
IF 4.825
Ji Wu,Ke Cao,Cai-Yan Zhang,Tao-Tao Xu,Xin-Yu Wen,Bo Li,Junxiao Yang
A palladium catalyzed selective B(3)–H activation/oxidative dehydrogenative coupling for the synthesis of bis(o-carborane)s connected with B(3)–B(3′) and B(3)–B(6′) bonds has been developed for the first time. A plausible mechanism involving stepwise activation of B(3)–H and B(3′/6′)–H bonds by PdII and PdIV was proposed. This work is the first example and the most efficient protocol for synthesis of bis(o-carborane)s connected with B(3)–B(3′) and B(3)–B(6′) bonds, which has important reference for design, synthesis, and application of bis(o-carborane)s in related fields.
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高影响因子
International Journal of Extreme Manufacturing
·
2022
·Vol. 5
·期 1
·015502
·
IF 10.036
Zhibin Jiang,Lujie Jin,Xiying Jian,Jinxia Huang,Hongshuai Wang,Binhong Wu,Kang Wang,Ling Chen,Youyong Li,Xiang Liu,Weishan Li
Lithium-sulfur (Li-S) batteries can provide far higher energy density than currently commercialized lithium ion batteries, but challenges remain before it they are used in practice. One of the challenges is the shuttle effect that originates from soluble intermediates, like lithium polysulfides. To address this issue, we report a novel laminar composite, N,O-carboxymethyl chitosan-reduced graphene oxide (CC-rGO), which is manufactured via the self-assembly of CC onto GO and subsequent reduction of GO under an extreme condition of 1 Pa and −50 °C. The synthesized laminar CC-rGO composite is mixed with acetylene black (AB) and coated on a commercial polypropylene (PP) membrane, resulting in a separator (CC-rGO/AB/PP) that can not only completely suppress the polysulfides penetration, but also can accelerate the lithium ion transportation, providing a Li-S battery with excellent cyclic stability and rate capability. As confirmed by theoretic simulations, this unique feature of CC-rGO is attributed to its strong repulsive interaction to polysulfide anions and its benefit for fast lithium ion transportation through the paths paved by the heteroatoms in CC.
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高影响因子
iScience
·
2023
·
IF 5.8
Luxian Guo,Yangming Ding,Hua Wang,Yuxuan Liu,Qian Qiang,Qi Luo,Fei Song,Changzhi Li
The catalytic conversion of lignin into N-containing chemicals is of great significance for the realization of value-added biorefinery concept. In this article, a one-pot strategy was designed for the transformation of lignin β-O-4 model compounds to imidazo[1,2-a]pyridines in yields up to 95% using 2-aminopyridine as a nitrogen source. This transformation involves highly coupled cleavage of C-O bonds, sp 3 C-H bond oxidative activation, and intramolecular dehydrative coupling reaction to construction of N-heterobicyclic ring. With this protocol, a wide range of functionalized imidazo[1,2-a]pyridines sharing the same structure skeleton as those commercial drug molecules, such as Zolimidine, Alpidem, Saripidem, etc., were synthesized from different lignin β-O-4 model compounds and one β-O-4 polymer, emphasizing the application feasibility of lignin derivatives in N-heterobicyclic pharmaceutical synthesis.
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高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 1
·127-135
·
IF 4.831
Huang Jing-Yi,Bao Ting,Hu Tian-Xing,Wen Wei,Zhang Xiu-Hua,Wang Sheng-Fu
A strategy was developed for the voltammetric determination of the antibiotic drug levofloxacin (LV) based on a glassy carbon electrode modified with a composite consisting of poly(o-aminophenol) and graphene quantum dots (PoAP/GQD) that was fabricated by electropolymerization. The PoAP/GQD composite provides a large surface area and sensing interface and strongly promotes the oxidation current of LV. Under optimal conditions, the modified GCE displays an oxidation peak current (best measured at a working voltage of 1.05 V vs. SCE) that is linearly related to the levofloxacin concentration in the range from 0.05 to 100 μM, and the detection limit is 10 nM (at an S/N of 3). The method was applied to the determination of levofloxacin in spiked milk samples where is gave recoveries between 96.0 and 101.0 %.
We describe a one-step electrochemical polymerization method to synthesize a layer of conductive film of poly(o-aminophenol) and graphene quantum dots (PoAP/GQD) onto a glassy carbon electrode (GCE) surface. The composite film exhibited high electro catalytic activity for the quantitative determination of levofloxacin by stripping voltammetry.
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高影响因子
Materials Chemistry Frontiers
·
2019
·Vol. 4
·期 1
·257-267
·
IF 0
Xueyan Wu,Jixi Guo,Yan Lv,Dianzeng Jia,Jianzhang Zhao,Huici Shan,Xiaoping Jin,Yongdong Ma
o-Carborane was used to alter the electronic states of π-conjugated organic aryls and was demonstrated as an effective optical and electronic control unit to tune the photophysical properties, endowing the molecules with certain luminescence properties and modifying the HOMO and LUMO energies for electron transfer. We introduced fluorene and its heteroanalogs (carbazole, dibenzofuran and dibenzothiophene), four types of substitutes with different electron-rich or electron-deficient heteroatoms, to the two carbon atoms of o-carborane to synthesize CDC, CDN, CDO and CDSvia a modified nickel-catalyzed cross-coupling reaction. The photophysical properties of o-carborane-containing heterocyclic compounds were investigated and compared with each other. The results showed that all compounds were AIE luminogens with tunable fluorescence colors from blue to yellow emissions (λem = 465–570 nm) and possessed excellent emission quantum efficiencies in the solid state (ΦF > 70%). Among the four o-carborane-containing heterocyclic compounds, the ground state structures confirmed by crystallographic measurements provided a close correlation of the structure and electronic properties between the π-conjugated organic aryl groups and o-carborane. Besides, the heteroatoms controlled and altered the electronic interaction between aryls and o-carborane due to the different electron-rich or electron-deficient attributes. The HOMO and LUMO energies were estimated by DFT calculations. The excited states were calculated by TD-DFT and correlated with the corresponding absorption and emission spectra to systematically elucidate the electronic perturbation. This work not only demonstrates an efficient strategy for constructing o-carborane-containing heterocyclic AIE luminogens but also indicates that they are promising as advanced optoelectronic materials.
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