BIOMACROMOLECULES
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2025
·
IF 5.4
Qianru Li,Yuhe Zhu,Jiahui Ding,Weizhen Pan,Yuwei Hu,Jiao Lu,Sihai Gao,Hongyang Jiang,Zhongyuan Yin,Songwei Tan
Gene therapy is often limited by gene vectors. To reduce the impact of intracellular reactive oxygen species and improve transfection efficiency, thioether bonds were introduced in poly(β-amino ester) (PBAE), a well-known nonviral gene vector, to synthesize PBAE-poly(β-thioether ester) copolymer (PBAE-TE). By varying the monomer feeding ratio and reaction time, the optimized copolymer (PBAE-TE2–5) showed the highest transfection efficiency in SiHa and HeLa cells (75.00 and 78.70%) with a 75:1 copolymer/GFP ratio. PBAE-TE2–5 also demonstrated superior transfection performance compared to PBAE in multiple cell lines (HeLa, Me180, C666–1, and HEK 293T) and better penetration capability in 3D spheroid cell models (SiHa and HEK 293T). The PBAE-TE2–5-CRISPR/Cas9 plasmid polyplexes could effectively edit target genes in HEK 293T (MUC2) and SiHa (HPV16 E7) cells, and inhibit cancer cell proliferation both in vitro and in vivo. These findings highlight the promising potential of PBAE-TE as an effective gene delivery system.
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Journal of Functional Foods
·
2024
·
IF 3.8
Ya-wen Zhang,Yue-ru Shen,Hong-yu Zhang,Huai-tong Yao,Bing Hu
Sleep is essential to animals with circadian rhythms. Sleep deprivation causes serious pathophysiological consequences, such as DNA damage and oxidative stress. Pyrroloquinoline quinone (PQQ) is a bioactive food ingredient with antioxidant properties. Here, we used zebrafish continuous swimming paradigm sleep deprivation model to investigate the effect of PQQ on DNA damage. We found that DNA damage and neuronal activity increase following sleep deprivation. PQQ pretreatment reduced DNA damage in the zebrafish dorsal pallium. As an antioxidant, PQQ effectively inhibited reactive oxygen species (ROS) production induced by 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH) and sleep deprivation, and upregulated the expression of antioxidant genes sod1 and gpx4b . Also, PQQ ameliorated the decreased silent information regulator 1 (SIRT1) expression level, suggesting that PQQ may remove excessive ROS by activating SIRT1, thereby reducing DNA damage within neurons. This study indicates that PQQ has strong antioxidant function, provides ideas for further development of ingredients for sleep recovery.
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ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xu Fu,Wen Yin,Dao Shi,Yifan Yang,Suisui He,Jun Hai,Zhenqing Hou,Zhongxiong Fan,Dekui Zhang
The synergistic nanotheranostics of reactive oxygen species (ROS) augment or phototherapy has been a promising method within synergistic oncotherapy. However, it is still hindered by sophisticated design and fabrication, lack of a multimodal synergistic effect, and hypoxia-associated poor photodynamic therapy (PDT) efficacy. Herein, a kind of porous shuttle-shape platinum (IV) methylene blue (Mb) coordination polymer nanotheranostics-loaded 10-hydroxycamptothecin (CPT) is fabricated to address the abovementioned limitations. Our nanoreactors possess spatiotemporally controlled O2 self-supply, self-sufficient singlet oxygen (1O2), and outstanding photothermal effect. Once they are taken up by tumor cells, nanoreactors as a cascade catalyst can efficiently catalyze degradation of the endogenous hydrogen peroxide (H2O2) into O2 to alleviate tumor hypoxia. The production of O2 can ensure enhanced PDT. Subsequently, under both stimuli of external red light irradiation and internal lysosomal acidity, nanoreactors can achieve the on-demand release of CPT to augment in situ mitochondrial ROS and highly efficient tumor ablation via phototherapy. Moreover, under the guidance of near-infrared (NIR) fluorescent imaging, our nanoreactors exhibit strongly synergistic potency for treatment of hypoxic tumors while reducing damages against normal tissues and organs. Collectively, shuttle-shape platinum-coordinated nanoreactors with augmented ROS capacity and enhanced phototherapy efficiency can be regarded as a novel tumor theranostic agent and further promote the research of synergistic oncotherapy.
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Small
·
2025
·
IF 12.1
Xu Zhang,Zhichao Dou,Huayang Fan,Tu Huang,Zhuohao Zhang,Yaling Tang,Xin-hua Liang
Reactive oxygen species (ROS) can catalyze the palmitoylation of Gasdermin D (GSDMD), thereby promoting pyroptosis. However, tumor cells frequently exhibit elevated expression of heat shock proteins (HSPs), which attenuate pyroptosis through the inhibition of key pyroptotic mediators. To solve these problems, a folate-acid-modified polydopamine nanoparticle loaded with copper peroxide (PDCF nanoparticles) is developed to simultaneously trigger and amplify pyroptosis while suppressing HSPs-mediated defensive mechanisms. PDCF nanoparticles efficiently elevated intracellular ROS, thereby directly promoting the palmitoylation and subsequent cleavage of GSDMD to its active N-terminal GSDMD (GSDMD-N). This process created a positive feedback loop that enhanced pyroptosis, characterized by pore formation in the plasma membrane and the release of interleukin 1β (IL-1β) and interleukin 18 (IL-18). Concurrently, the elevated ROS significantly downregulated HSPs expression to achieve a mild photothermal therapy (MPTT). This combined strategy of pyroptosis amplification and HSPs inhibition potently suppressed tumor growth in oral squamous cell carcinoma (OSCC). The novel multifunctional therapeutic strategy provides a new view for the treatment of OSCC through ROS-mediated downregulation of HSPs and improving pyroptosis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Dalei Li,Yawen Yu,Rong Fan,Mingyan Yang,Yuxuan Zou,Fei Yao,Yami Wu,Yi Ge,Huaying Fan
Oral ulcers (OU) are prone to recurrence, and are often manifested by the accompanying bacterial infections which may induce a vicious cycle of oxidative stress and inflammatory responses. Traditional treatment methods have limited effects, including being eliminated in the moist and dynamic environment of the mouth, and are prone to various side effects or the development of drug resistance. In this study, we designed borate ester-based and Schiff base-cross-linked hydrogel (OPEC), and loaded ginseng-derived exosome (GEX@OPEC) for the treatment of OU. OPEC hydrogel is composed of phenylboronic acid-functionalized hyaluronic acid oxide (OHA-PBA), epigallocatechin gallate (EGCG), and carboxymethyl chitosan (CMCS). The addition of EGCG enhances its borate cross-linking network and improves the adhesion of the hydrogel. GEX is connected to OPEC through hydrogen bonds and loaded into the hydrogel, further enhancing its gel network. GEX@OPEC exhibited excellent biocompatibility and degradability, ensuring safe oral use of the hydrogel. GEX@OPEC can release EGCG and GEX in response to pH and ROS to exert therapeutic effects. Our research results showed that GEX@OPEC had excellent antibacterial properties, anti-inflammatory and antioxidant stress effects, as well as angiogenic properties, which can effectively promote the healing of OU in diabetic rat models simulating OU. To sum up, GEX@OPEC has good application prospects in the treatment of diabetic OU.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yunfei Shen,Huaiqi Shi,Keying Qu,Zuopeng Li,Bolong Yang,Zhansheng Wu
Multifunctional cotton fabric holds significant promise for domestic and healthcare applications. A key challenge remains developing sustainable fabrication routes for mechanically durable cotton textiles integrating intrinsic and photothermally amplified antibacterial functions. Herein, we prepared ZIF-8/67-loaded Ag nanoparticles on cotton fabric using in-situ reduction technology (ZIF-8/67-Ag@CCT). Subsequent dip-coating with polydimethylsiloxane (PDMS) produced ZIF-8/67-Ag@CCT@PDMS, enhancing superhydrophobicity. The modified fabric exhibited exceptional superhydrophobicity (water contact angle: 148.55 ± 0.21°) and UV-blocking capabilities (UPF value: 186). The surface demonstrated excellent self-cleaning properties against dyes and maintained robust stability after 30 washing cycles and immersion in acidic/alkaline solutions (>97.0% antibacterial efficacy retention). Critically, the fabric exhibited excellent biocompatibility, as validated by human induced pluripotent stem cell-derived skin organoid assays showing no adverse effects on cell proliferation, apoptosis, or survival rates. Antibacterial effects originated from the penetration of AgNPs and Ag⁺/Zn²⁺/Co²⁺ ions into bacterial structures, disrupting metabolism via reactive oxygen species (ROS) generation. The multifunctional ZIF-8/67-Ag@CCT@PDMS fabric presents a highly promising solution for durable antibacterial medical textiles, offering combined antibacterial activity, exceptional stability, self-cleaning, UV resistance, and biocompatibility.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Xuejun Chen,Shaoyue Li,Qing Lu,Yitong Li,Mingrui Zhu,Shen Zhang,Yan Fang,Congjian Wen,Daohui Yang,Peili Fan,Huixiong Xu,Haohao Yin
Immunogenic cell death (ICD) significantly boosts anti-tumor immunotherapy effectiveness; however, the recruitment of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), driven by factors such as adenosine accumulation and oxygen depletion during ICD, impairs the overall therapeutic outcome as well as facilitates tumor development and metastasis. Recent studies identify CD300ld as a key regulator of PMN-MDSCs recruitment, making it a promising immunotherapeutic target. Here, an innovative strategy is developed to eminently amplify ICD while alleviating PMN-MDSC infiltration upon ultrasound (US) stimulation. A modified generation 5 (G5) poly(amidoamine) dendrimer (G5PBA) was employed to encapsulate hematoporphyrin (GH)-a widely used organic sonosensitizer-and modified the complex with pardaxin peptides (Par) to achieve precise endoplasmic reticulum (ER) targeting, and adsorbed the negatively charged siCD300ld (PGH@siRNA). Under US irradiation, PGH@siRNA precisely accumulates at ER to induce localized reactive oxygen species (ROS) bursts, and effectively silence CD300ld, thereby amplifying endoplasmic reticulum stress (ERS)-mediated ICD and reducing PMN-MDSCs infiltration to reshape the tumor microenvironment. This systematic preclinical evaluations demonstrated enhanced immune activation, suppressed metastasis, and improved therapeutic outcomes. This study introduces a sono-responsive synergistic strategy integrating ER-targeted sonodynamic therapy with gene silencing, offering a novel paradigm for targeting PMN-MDSCs and enhancing triple-negative breast cancer (TNBC) immunotherapy.
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Materials Today Bio
·
2025
·
IF 10.2
Haozhe Ren,Peisheng Liu,Ziang Sun,Zhe Yu,Hao Guo,Xinyue Cai,Yihang Wei,Zihan Li,Meiling Wu,Xinyue Xu,Jing Wang,Kun Xuan
Periodontal disease, a chronic inflammatory condition driven by dysbiotic biofilms and host immune dysregulation, lacks therapeutic strategies that simultaneously eliminate infection, resolve inflammation, and promote tissue regeneration. To bridge this gap, we designed a spatially compartmentalized dual-module nanosystem with ‘‘one cerium, dual functions’’. Specifically, the antibacterial module utilizes hyaluronic acid (HA)-modified single-atom cerium nanozymes (Ce-N-C@HA) delivered to periodontal pockets. Upon HA degradation by bacterial hyaluronidase, the exposed catalytic sites generate hydroxyl radicals (·OH) via peroxidase like (POD-like) activity, enabling targeted pathogen eradication. Concurrently, the immunomodulatory module delivers Lactobacillus reuteri -derived extracellular vesicles (EVs) loaded with ultra-small CeO 2 nanoparticles (CeO 2 -EV) into subgingival tissues. This module efficiently scavenges reactive oxygen species (ROS) and induces macrophage polarization to M2 anti-inflammatory phenotype, significantly alleviating the inflammatory microenvironment. By utilizing cerium's dual functionalities which localized antibacterial action and reconstructs immune homeostasis, this system improves the periodontal microenvironment and promotes tissue regeneration, providing a novel strategy for nanotherapy in oral inflammatory diseases.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Lei Nie,Xiaoyue Ding,Letian Yan,Man Li,Peng Ding,Oseweuba Valentine Okoro,Armin Shavandi
In recent years, hydrogels based on decellularized extracellular matrix (dECM) from tendons have been used in tissue scaffold preparation and guided tissue regeneration. However, pure dECM hydrogel may be limited in biomedical applications due to slow gelation kinetics, poor mechanical properties, and other suboptimal characteristics. To enhance functionality, dECM hydrogel can be remodelled using natural polysaccharide polymers. In this study, carboxymethyl chitosan (CMCS) and oxidized pullulan (OPL) were employed to remodel dECM synthesized from porcine Achilles tendon, resulting in the fabrication of dECM-based composite hydrogels (CLD). Scanning electron microscopy (SEM) images and X-ray computed microtomography (Micro-CT) confirmed the interconnected microstructure of CLD hydrogels. Furthermore, the CLD hydrogels exhibited favorable self-healing ability and adhesion properties. In vitro hemolysis test using the red blood cells demonstrated good hemocompatibility of CLD hydrogels. CLD hydrogels exhibited favorable antibacterial capabilities against Escherichia coli and Staphylococcus aureus, as well as effective reactive oxygen species (ROS) scavenging capabilities and good cytocompatibility. Collectively, these findings suggested that the fabricated dECM-based remodelling hydrogels hold potential in future biomedical applications.
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ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Hailun Zhao,Jian Ke,Shangkun Zhu,Manting Li,Jianqiu Chen,Qiulian Yang
The performance of Fe-catalyzed electrochemical advanced oxidation in antibiotic degradation was typically hampered by poor reduction efficiency of Fe 3+ to Fe 2+ and a narrow pH working range. This work employed natural hematite (HM) as iron source and oxalic acid (OA) as complexing agent in conjunction with electrolysis (EC) to address these concerns. Results showed that the EC/HM/OA system can achieve a 99.77 % degradation efficiency of sulfamethoxazole (SMX) under the optimized reaction conditions. The SMX degradation rate constant in EC/HM/OA system was 2.53-fold higher than in EC/HM system, following pseudo first-order kinetics. The OA addition promoted the Fe 3+ reduction by complexing with HM in electrochemical system, as well as extended the pH application range of EC/HM system to 3 − 11. The ATR−FTIR test and DFT calculation demonstrated that the adsorption of OA on the HM surface occurred in a deprotonated bidentate mononuclear configuration. Radical quenching experiments and EPR analysis confirmed that the crucial reactive oxygen species for SMX degradation in EC/HM/OA system were the ·OH and ·O 2 - radicals. Five potential degradation pathways of SMX were proposed. ECOSAR model and Daphnia magna toxicity tests demonstrated attenuated toxicity of SMX after degradation. The EC/HM/OA system exhibited robust resistance to common anions (Cl⁻ and NO₃⁻), and achieved high SMX degradation (86.82 %–99.67 %) across different water matrices. The combination of natural HM with OA significantly improved the degradation performance of electrochemical process for persistent antibiotics.
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Materials Today Bio
·
2025
·
IF 10.2
Jiayi Zuo,Yining Pan,Yuanli Wang,Wei Wang,Haojie Zhang,Si Zhang,Youru Wu,Jiangfan Chen,Qingqing Yao
Neovascular age-related macular degeneration (nAMD) has become the leading cause of vision loss in people over 60 years old. Anti–vascular endothelial growth factor (anti-VEGF), the current first-line drug for the treatment of nAMD, suffers from poor patient compliance and fundus fibrosis scar formation. In addition to VEGF, oxidative stress and inflammation also play key roles in the pathological process of choroidal neovascularization (CNV). Therefore, combinational therapeutics with anti-angiogenic, reactive oxygen species (ROS)-scavenging and anti-inflammatory functions will broaden therapeutic effects and reduce side effects. The Yes-associated protein-1 (YAP) has proven to inhibit angiogenesis, inflammation, and subretinal fibrosis in CNV. Herein, verteporfin (VP), the inhibitor of YAP, was encapsulated into a polydopamine modified mesoporous silica nanoparticle (PMSN-VP NPs) and then conjugated with PLGA-PEG-PBA decorated cerium oxide nanoparticles (PPCeO 2 NPs) to develop an integrated nano-drug delivery system. The PMSN-VP@PPCeO 2 NPs exhibited ROS-responsive degradation and VP release behaviors, and our in vitro data revealed that the PMSN-VP@PPCeO 2 NPs downregulated angiogenic-related and fibrosis-related gene expressions in human umbilical vein endothelial cells (HUVECs) and further showed excellent anti-oxidative and anti-inflammatory capacities in BV2 cells. More importantly, the PMSN-VP@PPCeO 2 NPs significantly suppressed vascular leakage and macrophage infiltration in the laser-induced CNV lesions of mice. Overall, our findings demonstrated that the PMSN-VP@PPCeO 2 NPs provided an effective therapeutic strategy for nAMD.
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METABOLIC BRAIN DISEASE
·
2025
·Vol. 40
·期 5
·1-14
·
IF 3.5
Zhao Hexiang,Yang Ping,Zhang Mou,Zheng Wenshu,Qi Renli,Zhu Xiaofeng,Li Jinghui,Li Shipeng
Reperfusion, while essential for restoring blood supply, paradoxically exacerbates neuronal damage through cerebral ischemia-reperfusion injury (CIRI). This study aimed to develop a reactive oxygen species (ROS)-responsive drug delivery system (DDS) loaded with edaravone (EDA) to enhance targeted therapy for CIRI. The stimuli-responsive DDS was synthesized using dextran (DEX) as the biocompatible carrier and benzeneboronic acid pinacol ester (BAPE) as the ROS-sensitive moiety. The physicochemical characteristics of the DEX-BAPE/EDA (DB/EDA) micelles were systematically evaluated. In vitro studies assessed the anti-inflammatory, antioxidant, and anti-apoptotic effects of DB/EDA. Moreover, the neuroprotective efficacy of DB/EDA in vivo was analyzed via behavioral tests, infarct volume measurement, ELISA assays of inflammatory cytokines and OS markers, and Western blot analysis of Nrf2-related pathways. Pharmacokinetics and biosafety were analyzed through plasma profiling and H&E staining. DB/EDA exhibited high stability, efficient drug encapsulation, and ROS-responsive drug release. Cellular uptake studies confirmed enhanced internalization of DB/EDA micelles in BV2 cells. In the oxygen-glucose deprivation/reoxygenation (OGD/R) model, DB/EDA significantly suppressed TNF-α, IL-1β, IL-6, and MDA, restored SOD levels, and attenuated apoptosis. In the middle cerebral artery occlusion/reperfusion (MCAO/R) mice, DB/EDA administration effectively improves cognition and mitigates neuronal damage. Mechanistically, DB/EDA activated the Nrf2/HO-1 pathway, amplifying antioxidant and anti-inflammatory responses. Pharmacokinetic analysis revealed prolonged circulation and increased brain accumulation, and histopathological analysis demonstrated the safety profile of DB/EDA. The ROS-responsive DB/EDA nano-micelles provided targeted EDA delivery to ischemic brain regions, alleviating CIRI via Nrf2 activation, suggesting that DB/EDA is a promising strategy for CIRI treatment. Graphical abstract
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