Journal of Materials Chemistry B
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2015
·Vol. 3
·期 48
·9383-9396
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IF 4.726
Xin Liang,Ying Yang,Lijun Wang,Xianbing Zhu,Xiaowei Zeng,Xiaojin Wu,Hongbo Chen,Xudong Zhang,Lin Mei
Poly(lactide-co-glycolide) (PLGA)-based particles have been widely used as carriers of various kinds of drugs, which are sequestered by the cell membrane and degraded through endo-lysosome and auto-lysosomal pathways. Lysosome is the destination of endocytosis and autophagy, which is also an organelle for the cell to execute death. Here, we show that chloroquine (CQ) and ciprofloxacin (CPX) (LMP inducer reagents)-loaded PLGA hollow microspheres (HMs) could be delivered by passive targeting into endo-lysosome and auto-lysosome. Co-loading with NaHCO3 accelerates the release of CQ and CPX in the acid environment of endo-lysosome and auto-lysosome. Subsequently, the released CQ and CPX induce lysosomal membrane permeabilization (LMP), which leads to cancer cell death in three different manners: apoptosis, autophagic cell death and apoptosis with autophagosome. Moreover, we use rapamycin, the inhibitor of the mammalian target of rapamycin (mTOR), to induce autophagy and inhibit cell growth. Rapamycin–NaHCO3-loaded HMs combined CQ–NaHCO3-loaded HMs could efficiently induce cancer cell death through apoptosis with autophagosome both in vitro and in vivo.
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高影响因子
Polymer Chemistry
·
2015
·Vol. 6
·期 11
·2069-2075
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IF 5.52
Fan Jia,Yin Wang,Haibo Wang,Qiao Jin,Tongjiang Cai,Yangjun Chen,Jian Ji
Ideal drug delivery systems are required to be stable in circulation, while instable at targeted sites to induce rapid drug release. To address this dilemma, novel light cross-linkable and pH de-cross-linkable micelles were developed as drug nanocarriers. Poly(ethylene glycol)-poly-(hydrazone umbelliferone)-aspartamide (mPEG-OMC) with light responsive coumarin moieties and pH responsive hydrazone groups in the side chains was synthesized. The polymers can self-assemble into micelles, which can be conveniently stabilized via UV-light induced cross-linking based on the dimerization of coumarin moieties. While under slight acidic conditions, the cross-linked micelles can also be de-cross-linked by the cleavage of hydrozone bonds in the cross-linking segments, accelerating the release of encapsulated drugs. Thus the benefits of pH and light stimuli-responsiveness can be combined and their disadvantages are avoided. The doxorubicin-loaded micelles showed high mortality towards HepG2 cells. We have demonstrated the UV light cross-linkable micelles with pH-triggered de-cross-linking properties as a new approach for designing smart drug delivery systems.
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Acta Biomaterialia
·
2017
·
IF 6.319
Jiao Guan,Zun-Qiang Zhou,Mao-Hua Chen,Hui-Yan Li,Da-Nian Tong,Jun Yang,Jing Yao,Zheng-Yun Zhang
In this study, we developed a folate (FA)-conjugated and pH-responsive active targeting micellar system for anti-cancer drug delivery. In this system, FA was attached to the terminal of the hydrophilic segment of poly(lactic acid)–poly( L -lysine) (PLA–PLL), and PLL was modified by a citric acid group. The FA receptor-mediated active targeting and electrostatic interaction between micelles and cell membrane due to a negative-to-positive charge reversal was combined in one micellar anti-cancer drug delivery system to enhance the tumour targeting and cellular internalisation of micelles. In vitro and in vivo anti-cancer studies demonstrated that the doxorubicin-loaded, FA-conjugated and pH-responsive polymeric micelles possess an enhanced and effective cancer efficiency. Statement of significance Negatively charged nano-carriers prolonged anti-cancer drugs’ blood circulation. However it is difficult to be internalised. Therefore, a negative-to-positive charged micelle surface could improve selectivity for tumour cells and increase uptake chance. In this study, we developed a folate (FA)-conjugated and pH-responsive active targeting micellar system for anti-cancer drug delivery. The FA receptor-mediated active targeting and electrostatic interaction between micelles and cell membrane due to a negative-to-positive charge reversal was combined in one micellar anti-cancer drug delivery system to enhance the tumour targeting and cellular internalisation of micelles. In vitro and in vivo anti-cancer studies demonstrated that the doxorubicin-loaded, FA-conjugated and pH-responsive polymeric micelles possess an enhanced and effective cancer efficiency.
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Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Jianbing Wu,Xusheng Xie,Zhaozhu Zheng,Gang Li,Xiaoqin Wang,Yansong Wang
The effects of changing solution pH in the range of 3.6–10.0 during a one-step silk microsphere preparation process, by mixing silk and polyethylene glycol (PEG), was assessed. The microspheres prepared at low pH (3.6) showed a more homogeneous size (1–3 μm) and less porous texture than those prepared at neutral pH. High pH (10.0) inhibited microsphere formation, yielding small and inhomogeneous microspheres. Compared to neutral pH, low pH also increased the content of silk crystalline β-sheet structure from approx. 30% to above 40%. As a result, the microspheres produced at low pH were more thermally stable as well as resistant to chemical (8 M urea) and enzymatic (protease XIV) degradation when compared to microspheres prepared at neutral pH. Doxorubicin hydrochloride (DOX) and curcumin (CUR) were successfully loaded in silk microspheres via control of solution pH. The loading efficiency of DOX was approx. 95% at pH 7.0 and approx. 60% for CUR at pH 3.6, attributed to charge-charge interactions and hydrophobic interactions between the silk and drug molecules, respectively. When PBS, pH 7.4, was used as a medium for release studies, the pH 3.6 microspheres released both drugs more slowly than the pH 7.0 microspheres, likely due to the high content of crystalline β-sheet structure that enhanced drug-silk interactions as well as restricted drug molecule diffusion.
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高影响因子
Biomaterials Science
·
2015
·Vol. 4
·期 2
·338-345
·
IF 3.831
Sheng Wang,Weitao Yang,Jing Cui,Xue Li,Yan Dou,Lin Su,Jin Chang,Hanjie Wang,Xiaodong Li,Bingbo Zhang
The side effects of antitumor drugs and low treatment efficacy are two major challenges of current chemotherapy. To address these issues, we developed a new kind of smart nanocarriers that combine pH-responsive chemotherapy and near-infrared (NIR) light triggered photodynamic therapy. These nanocarriers were based on upconversion nanoparticle (UCN)-loaded folate-conjugated polymeric lipid vesicles (UFPLVs). Merocyanine 540 (MC540), as a photosensitizer, was loaded in the UFPLVs; doxorubicin hydrochloride (DOX), as an antitumor drug, was conjugated to the surfaces of UFPLVs by pH-sensitive hydrazone bonds. The newly developed MC540&DOX–UFPLVs had a nanosized structure with targeting ligand modification, so they had the potential to accumulate into tumor sites via a combination of passive and active targeting effects. An in vitro singlet oxygen test showed that the nanocarriers can generate cytotoxic singlet oxygen successfully under the irradiation of NIR light. In vitro DOX release profiles demonstrated that the nanocarriers can achieve a pH-triggered drug release. It has been demonstrated by a cellular uptake study that the nanocarriers can efficiently deliver drugs and photosensitizers into tumor cells. In vitro and in vivo combination treatments evidenced the high antitumor effects of MC540&DOX–UFPLVs under NIR light irradiation. These results suggest that the MC540&DOX–UFPLVs may be promising nanocarriers for tumor combination therapy applications.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2017
·Vol. 35
·期 8
·924-938
·
IF 1.692
Lu Bei-bei,Wei Lu-lu,Meng Gui-hua,Hou Jun,Liu Zhi-yong,Guo Xu-hong
Well-defined pH-responsive poly( ε -caprolactone)-graft- β -cyclodextrin-graft-poly(2-(dimethylamino)ethylmethacrylate)- co -poly(ethylene glycol) methacrylate amphiphilic copolymers (PCL- g - β -CD- g -P(DMAEMA- co -PEGMA)) were synthesized using a combination of atom transfer radical polymerization (ATRP), ring opening polymerization (ROP) and “click” chemistry. Successful synthesis of polymers was confirmed by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ( 1 H-NMR), and gel permeation chromatography (GPC). Then, the polymers could selfassemble into micelles in aqueous solution, which was demonstrated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The pH-responsive self-assembly behavior of these copolymers in water was investigated at different pH values of 7.4 and 5.0 for controlled doxorubicin (DOX) release, and these results revealed that the release rate of DOX could be effectively controlled by altering the pH, and the release of drug loading efficiency (DLE) was up to 88% ( W / W ). CCK-8 assays showed that the copolymers had low toxicity and possessed good biodegradability and biocompatibility, whereas the DOX-loaded micelles remained with high cytotoxicity for HeLa cells. Moreover, confocal laser scanning microscopy (CLSM) images revealed that polymeric micelles could actively target the tumor site and the efficient intracellular DOX release from polymeric micelles toward the tumor cells further confirmed the anti-tumor effect. The DOX-loaded micelles could easily enter the cells and produce the desired pharmacological action and minimize the side effect of free DOX. These results successfully indicated that pH-responsive polymeric micelles could be potential hydrophobic drug delivery carriers for cancer targeting therapy with sustained release.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Chang Liu,Guijuan Wei,Honglei Chen,Weiqiang Sun,Xixia Zhao,Xin Zhao
Charge redistribution strategy driven by geometry regulation of metal cores or carbon defect engineering is an effective approach to optimize the electronic structure of traditional carbon, which can boost hydrogen evolution reaction (HER) activity to inert carbon. The synergistic effect of these two methods is anticipated to more effectively manipulate the charge distribution of carbon materials. Herein, a facile low-temperature methane plasma strategy is developed, from which highly dispersive hexagonal close-packed (hcp) NiPt alloy encapsulated in the defect-rich carbon shell grown on the Ni foam (C@hcp-NiPt/NF) can be fabricated. The optimized C@hcp-NiPt/NF displays low overpotentials of 60, 110, and 60 mV at 100 mA cm −2 in 1.0 M KOH, 1.0 M PBS, and 0.5 M H 2 SO 4 , respectively. Furthermore, it demonstrates exceptional stability under alkaline, neutral and acidic conditions. Raman spectroscopy, in-situ electrochemical impedance spectroscopy (EIS), combined with theoretical calculations have unequivocally validate that the incorporation of carbon defects and the regulation of geometric structure in NiPt cores significantly augment the charge density on the carbon shell, thereby optimizing the binding affinity of carbon with H 2 O and hydrogen, ultimately resulting in improved HER performance.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yunlei Yin,Cheng Guo,Qianqian Mu,Wanwan Li,Hongying Yang,Yin He
Textiles is an excellent candidate for the development of smart wearable electronic devices due to its superior comfort, breathability, and lightweight properties. The integration of flexible electronic devices onto fabric is particularly advantageous for the advancement of future smart wearables. In this study, we developed highly sensitive pH-sensing and temperature-sensing nanoyarns featuring a coaxial structure by combining conjugate spinning technology with in-situ polymerization techniques. The pH-sensing nanoyarns demonstrated remarkable pH sensitivity ((−47.8 mV/pH), rapid response times (10 s), and excellent linearity (R 2 = 0.993), while the temperature-sensing nanoyarns exhibited a temperature sensitivity of (−1.53 %/℃) along with notable anti-interference capabilities. Utilizing modern knitting technology, we wove cotton yarn together with sensing yarn to create an intelligent electronic fabric that retains high pH sensitivity (−35.3 mV/pH) and outstanding linearity (R 2 = 0.983). Furthermore, this electronic fabric showcased excellent temperature sensitivity (−1.21 %/°C), strong linearity (R 2 = 0.986), and remarkable cyclic stability across the dynamic range from 30 °C to 50 °C. This work not only expands the application of yarn-based sensors in flexible electronic textiles but also provides new application scenarios for monitoring daily human activities, pH, body temperature, and environmental temperature stimuli.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Fanjia Dai,Fengjiao Chen,Jiaying Zhang,Xianwu Chen,Hongze Liang,Zhenjiang Liang,Shun Zhang,Hui Tan,Lingling Zhao
The tumor microenvironment is distinguished from normal tissues, such as the acidic microenvironment, elevated reactive oxygen species (ROS) levels, and overexpressed specific receptors on the cell surface. According to these unique hallmarks of cancer, stimulus-responsive drug carriers can be designed for targeted anticancer drug delivery and specific drug release in tumor tissues. Herein, a folate-modified pH/ROS dual-responsive polymeric micellar nanosystem was developed for the targeted delivery of doxorubicin (DOX). The drug release profile suggested a sustained drug release from the polymeric micelles and an accelerated drug release at lower pH and higher ROS levels. The cellular uptake assay indicated that the polymeric micelles were internalized by HepG2 cells and that the cellular uptake of micelles can be enhanced by folate modification through a receptor-mediated endocytosis pathway. Furthermore, the folate-modified pH/ROS dual-responsive polymeric micellar nanocarriers demonstrated enhanced inhibition on the migration of HepG2 cells in the cell scratch test and showed improved suppression on the proliferation of HepG2 cells and a lower IC50 than the nonfolate micelles in the cell viability test, indicating the desirable in vitro anticancer efficiency of the nanosystem.
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高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Ning Li,Chang Liu,Li Niu,Xu Li,Jacko Feng,Zhiming Liu
In this study, we introduced an innovative method by incorporating 1-butyl-3-methylimidazolium chloride (BmimCl) as a versatile component and fine-tuning the mixture of chitosan, BmimCl, and cyanidin cation (Anth) to create pH-responsive smart films for food freshness. Owing to the dual functionality of BmimCl, it served as a plasticizer as well as enhanced the film’s capacity to display a broader spectrum of visible color changes. The material properties of chitosan films containing BmimCl and Anth at varying concentrations were characterized using FTIR, XRD, mechanical test, TGA, UV-vis and pH sensitivity. The combined results indicate that the chitosan-based pH-responsive smart film possessed excellent mechanical properties, great thermal stability, high light transmission, and a broad pH sensitivity. pH-responsive films were used for real-time freshness monitoring of freshwater shrimps, sterilized milk, and hairtails. Results revealed that BmimCl disrupts the hydrogen bond network of chitosan molecules. The films exhibit a transition from green to red as the food turns from fresh to spoiled, effectively demonstrating their capability to monitor freshness.
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Journal of Water Process Engineering
·
2024
·
IF 6.3
Guoqi Liu,Jihong Fu,Junli Yu,Yaxue Zhang,Ronglan Wu
pH-responsive smart materials with switchable wettability have a great potential application in bidirectional oil-water separation. In this work, a simple, low-cost and environmentally friendly strategy is developed to construct a pH-responsive melamine sponge (MS) with reversible wettability via polydopamine (PDA) modification followed by grafting long-chain alkanes with carboxyl (–COOH) and methyl (–CH 3 ) groups. The as-modified sponge exhibits unique pH-responsiveness, that is, the water contact angle (WCA) is measured to be 144.9° under acidic and neutral environments, while the wettability is radically changed from hydrophobic to hydrophilic under alkaline conditions. Moreover, in alkaline environment, the pH-responsive sponge can rapidly release the absorbed oil, demonstrating a controllable desorption performance. Also, the prepared sponge is utilized to accomplish controllable separation of various oil/water mixtures, as well as to effectively separate oil-in-water emulsions. Furthermore, after 50 cycles, the oil-water separation efficiency was still higher than 92 %. This smart wettability sponge has an extensive application prospect in controlled oil-water separation and on-demand oil-spill remediation.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zhilong Chen,Yan Guo,Xinyue Gu,Xinyu Liu,Jingjing Zhang,Chunyuan Song,Lianhui Wang
Interstitial fluid (ISF) is a prevalent accessible and information-rich biofluid, and monitoring pH level of interstitial fluid plays a crucial role in the physiological health assessment. However, facile extraction of ISF and sensitive detection of pH is still a great challenge. Herein, a flexible plasmonic microneedle array (PMNA)-based SERS sensor was developed for facile and sensitive pH monitoring of skin ISF. The flexible microneedle arrays were fabricated by norland optical adhesive 65 (NOA65), and coated with pH-sensitive 4-Mercaptobenzoic acid (4-MBA)-labeled silver nanoparticles (Ag NPs) by electrostatic assembly. The proposed flexible PMNA-based SERS sensor has a good linear response in the pH range of 5 to 9 which covers the normal pH level in skin interstitial fluid. The ISF extracted from porcine skin was chosen as the test model, and the pH levels detected by the PMNA-based SERS sensors were well consistent with the theoretical results. Besides, the proposed flexible SERS sensors show good mechanical robustness, sensing stability, and biocompatibility before and after skin insertion, which can be a potential analytical tool for practical detection of pH levels in ISF and provide technical support for SERS-based wearable biosensing.
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