INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Cuicui Duan,Hongru Wang,Yi Zhang,Hui Liu,Siyang Sui,Mengchun Qin,Xiaolei Li,Fumin Ma,Dan Li
Lactobacillus exopolysaccharides (EPS) have been widely used in fermented food and health care products due to their good biological activities. In this study, EPS derived from Lactobacillus plantarum A106 and β-lactoglobulin (β-Lg) were used to prepare β-Lg-EPS complex, and the structure and antioxidant activity of the complex were studied. The results indicated that the incorporation of EPS with β-Lg could quench the inherent fluorescence of β-Lg and change the conformation and secondary structure, finally improving the thermal stability. Moreover, addition of EPS changed the spherical structure of β-Lg, and the structure of β-Lg-EPS complexes appeared blocky and dendritic. Both ζ potential and particle size of β-Lg-EPS complex were decreased regardless of proportions between β-Lg and EPS. The dispersion system was the most uniform and stable when the ratio of β-Lg to EPS was 10:1. Moreover, β-Lg-EPS complex presented a higher free radical scavenging rate than that of native β-Lg, 64.47 ± 2.77 % for DPPH, 90.09 ± 0.42 % for ABTS + and 26.74 ± 0.56 % for ·OH, respectively. Moreover, β-Lg-EPS complex could alleviate oxidative damage and exert its antioxidant activity by improving cell viability and SOD activity, reducing ROS level, restoring mitochondrial membrane potential and improving apoptosis of LPS-induced RAW264.7 cells.
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Molecular Catalysis
·
2025
·
IF 4.9
Shiyu Wei,Yali You,Zixuan Wang,Dantong Feng,Ci Song,Yali Wang,Jiaxing Zhao,Yan Yang,Yanli Zheng,Yin Li,Wangshan Zheng,Yan Leng,Yongqiang Tian
β-Glucuronidase (GUS) catalyzes the hydrolysis of the glucuronide group in glycyrrhizin (GL) to produce glycyrrhetinic acid (GA). However, the industrial application of β-glucuronidases (GUSs) is often limited by their poor stability, low hydrolytic activity, and particularly insufficient substrate specificity. In this work, a novel and thermostable β-glucuronidase from Thermobacillus composti ( Tc GUS) was identified through gene mining. Evolutionary relationship and sequence analyses revealed that Tc GUS belongs to the family of typical GUS enzymes. Tc GUS demonstrated specificity and efficiently transformed GL to generate GA, exhibiting maximum activity at 35 °C and pH 6.5, with a half-life of 5 days at 50 °C, indicating excellent thermostability. The K m and V max values of Tc GUS were 3.20 mM and 151.18 μM·mg⁻¹·min⁻¹, respectively, reflecting its high substrate selectivity and biotransformation efficiency. Therefore, Tc GUS is a promising biocatalyst for the synthesis of glycyrrhetinic acid.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yaqi Liu,Chao Yuan,Bo Cui,Meng Zhao,Bin Yu,Li Guo,Pengfei Liu,Yishan Fang
In an effort to improve the application performance of apigenin, β-cyclodextrin metal–organic frameworks (BCDMOFs) known as porous materials were used to encapsulate apigenin via an innovative pH-adjusted method. The embedment efficiency had a significant positive pH dependence, reaching a maximum of 79.2 % ± 1.2 % at pH 12. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and thermogravimetric analysis demonstrated formation of apigenin/BCDMOFs composites, and exposure of BCDMOFs pores facilitated high embedment efficiency. Storage stability experiment and kinetic analysis showed degradation of apigenin/BCDMOFs composites was less than that of apigenin alone. Apigenin stability was increased by approximately 18 % after 7 days. BCDMOFs effectively encapsulated and controlled the release of apigenin, and the composites exhibited improved application performance in vitro .
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REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Simin Huang,Yizhong Yuan,Huimei Yu,Jinyu Sun,Xiaohui Tian,Chunhua Cai,Yuxuan Han,Yao Zhang
The remediation of wastewater contaminated with heavy metals remains a serious challenge due to the continuing threats posed to ecosystems and human health. While conventional methods such as membrane filtration and flocculation exist, developing sustainable adsorbents with high capacity and reusability is imperative. Therefore, we propose a novel strategy to overcome the limitations of natural polymer-based hydrogels by integrating β-FeOOH nanoparticles into sodium alginate matrices. FTIR, XPS and TG reveal that the incorporation of β-FeOOH not only increases the adsorption active sites but also improves the thermal stability of the composite hydrogels. The composite hydrogel exhibits exceptional adsorption capacity (581.40 mg/g). Crucially, the material maintains over 85 % of its initial efficiency after five adsorption-desorption cycles, demonstrating strong structural integrity and practical applicability. This work provides a mechanistic framework for designing multifunctional hydrogels through nanomaterial hybridization, offering a sustainable pathway for water purification technologies.
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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2025
·Vol. 29
·期 7
·e70539
·
IF 4.2
Tianyi Ji,Xiangying Li,Jiachen Li,Guan Wang
Wnt signalling is a highly conserved signalling pathway that plays an important role in a variety of biological processes. Frizzled (Fzd) family proteins are receptors for Wnt ligands. The physiological processes involved in mature trafficking of Fzd proteins remain elusive. Here, we identified asparagine residues 59 and 144 as the N-glycosylation modification sites of Fzd4. Sequence analysis of Fzd4 in different species showed that the two asparagine residues were highly conserved. N-glycosylation modification of Fzd4 is indispensable for its maturation and transport to the plasma membrane. N-glycosylation modification enhances the stability of Fzd4 and is also necessary for Fzd4 activity, which promotes Fzd4 interaction with Wnt ligands and co-receptor Norrin. Knockout of Fzd4 in the non-small cell lung cancer (NSCLC) cell line A549 followed by replenishment of Fzd4 glycosylation site mutants inhibited the growth and migration ability of A549 cells in vitro and in vivo. In summary, we identified N-glycosylation modification sites of Fzd4. N-glycosylation modification of Fzd4 is necessary for its stability and activity. When N-glycosylation modification is absent, Fzd4 cannot mediate the Wnt/β-catenin signalling pathway, which can inhibit the proliferation and migration of NSCLC and provide new targets and strategies for the treatment of NSCLC.
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Biology-Basel
·
2025
·Vol. 14
·期 10
·1358
·
IF 3.5
Bo Fan,Shunuan Fu,Yijun Zhu,Wei Tang,Yucai He
Simple SummaryThis study enhanced the relative activity of glycosyltransferase UGTBL1 through engineering mutations near its loop regions. The engineered enzyme efficiently catalyzed the glycosylation of the lignin-derived compoundp-hydroxybenzaldehyde (C7H6O2), enabling the environmentally benign synthesis of a Helicid analogue (p-hydroxybenzaldehydeβ-glucoside (C13H16O7)). Accordingly, this work provided novel insights for lignin valorization. Under optimized reaction conditions (35 °C, pH 7.5, 200 mM glucose (C6H12O6)), an exceptional yield of 97.8% forp-hydroxybenzaldehydeβ-glucoside was attained within 10 h using 2 mMp-hydroxybenzaldehyde substrate. Biotransformation of 3 mMp-hydroxybenzaldehyde (366.4 mg/L) afforded up to 2.7 mM of targetβ-glucoside product (767.5 mg/L). This research establishes a simpler, more economical, and sustainable enzymatic approach for the efficient synthesis ofp-hydroxybenzaldehydeβ-glucoside, offering significant potential benefits for pharmaceutical, cosmetic, or agricultural applications.Lignin, as one of the three primary components of renewable lignocellulosic biomass, can be converted into aromatic platform chemicals and holds significant potential for high-value applications.p-Hydroxybenzaldehyde is a compound derived from lignin. In this study, the mutant Δ60 of the glycosyltransferase UGTBL1 derived fromBacillus licheniformiswas adopted to catalyze the glycosylation reaction ofp-hydroxybenzaldehyde, producing a bioactive compound Helicid analogue (p-hydroxybenzaldehydeβ-glucoside). Truncation mutations targeting loop regions may reduce local flexibility, thereby facilitating enhanced access ofp-hydroxybenzaldehyde to the active site pocket and promoting relative activity. Under optimal conditions (35 °C, pH 7.5, and glucose 200 mM), a high yield of 97.8% forp-hydroxybenzaldehydeβ-glucoside was achieved from 2 mMp-hydroxybenzaldehyde within 10 h. The conversion of 3 mMp-hydroxybenzaldehyde (366.4 mg/L) yielded up to 2.7 mM (767.5 mg/L) ofp-hydroxybenzaldehydeβ-glucoside within 48 h. According to the molecular docking results, the CDOCKER energy value of mutant Δ60 was lower than that of the wild-type, at −16.0 kcal/mol. To our knowledge, this is the first example of an efficient and environmentally sustainable approach for the synthesis ofp-hydroxybenzaldehydeβ-glucoside, providing a new insight for the valorization of lignin into valuable biobased chemicals.
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International Journal of Biological Sciences
·
2024
·
IF 8.2
Zuodong Xuan,Chen Chen,Huimin Sun,Kunao Yang,Jinxin Li,Meilin Fu,Yang Bai,Zeyuan Zheng,Yue Zhao,Chunlan Xu,Bin Liu,Tian Li,Chen Shao
Background: Prostate cancer progression hinges on β-catenin's stability and activity, a key factor in epithelial-mesenchymal transition (EMT) and metastasis. This study delves into NDR1-dependent phosphorylation's impact on β-catenin via FBXO11, an E3 ubiquitin ligase, in prostate cancer cells. Methods: Human prostate cancer cell lines underwent various in vitro assays, including real-time PCR, Western blotting, immunoprecipitation, immunofluorescence, and protein stability assays, to explore β-catenin's interactions and post-translational modifications. NDR1 modulation's in vivo efficacy was assessed using a nude mice lung metastasis model. Small-molecule screening identified a potential NDR1 activator, aNDR1, tested for its effects on metastasis via in vitro and in vivo assays. Results: NDR1 phosphorylated β-catenin at Ser33/37, facilitating its interaction with FBXO11. This led to FBXO11-mediated ubiquitination and cytoplasmic degradation of β-catenin, while the NDR1-FBXO11 complex impeded β-catenin nuclear translocation by inducing JNK2 ubiquitination. Thus, NDR1 and FBXO11 jointly regulate β-catenin activity in prostate cancer cells through dual phosphorylation-driven ubiquitination, potentially suppressing EMT. Reduced NDR1 expression inhibited FBXO11 and β-catenin phosphorylation, diminishing β-catenin and JNK2 ubiquitination, promoting EMT and enhancing prostate cancer cell metastasis. The inhibitory effects of aNDR1 on prostate cancer metastasis were validated. Conclusion: The NDR1/FBXO11 axis outlines a non-canonical β-catenin degradation pathway crucial in regulating EMT and prostate cancer cell metastasis. NDR1 activation, particularly with aNDR1, could offer a promising therapeutic avenue against prostate cancer metastasis.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2020
·Vol. 44
·期 1
·67-79
·
IF 2.419
Zhou Hai-Yan,Zhou Jian-Bao,Yi Xiao-Nan,Wang Yan-Mei,Xue Ya-Ping,Chen De-Shui,Cheng Xin-Ping,Li Mian,Wang Hong-Yan,Chen Kai-Qian,Liu Zhi-Qiang,Zheng Yu-Guo
To develop new cellulases for efficient utilization of the lignocellulose, an endoglucanase (CoCel5A) gene from Colletotrichum orchidophilum was synthesized and a recombinant Pichia pastoris GS115/pPIC9K/cocel5A was constructed for secretory expression of CoCel5A. After purification, the protein CoCel5A was biochemically characterized. The endoglucanase CoCel5A exhibited the optimal activity at 55–75 °C and high thermostability (about 85% residual activity) at the temperature of 55 °C after incubation for 3 h. The highest activity of CoCel5A was detected when 100 mM citric acid buffer (pH 4.0–5.0) was used and excellent pH stability (up to 95% residual activity) was observed after incubation in 100 mM citric acid buffer (pH 3.0–6.0) at 4 °C for 24 h. Carboxymethyl cellulose sodium salt ( n = approx. 500) (CMC) and β - d -glucan were the best substrates for CoCel5A among the tested substrates. The kinetic parameters V max , K m , and K cat / K m values against CMC were 290.70 U/mg, 2.65 mg/mL, and 75.67 mL/mg/s, respectively; and 228.31 U/mg, 2.06 mg/mL, and 76.45 mL/mg/s against β - d -glucan, respectively, suggesting that CoCel5A has high affinity and catalytic efficiency. These properties supported the potential application of CoCel5A in biotechnological and environmental fields.
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IBRO Neuroscience Reports
·
2025
·
IF 2.9
Penghui Cao,Shichuan Shen,Ziyan Fang,Junjie Tan,Jinwei Wang,Nannan Pan
Objective This study aims to investigate the role of the TGF-β/Smad Signaling in depression comorbidity in epileptic individuals and to evaluate the effect of losartan on depressive symptoms. Methods Li-Popilocarpine was used to induce status epilepticus (SE) in rats. The rats in which SE was successfully induced were randomly divided into the SE saline group and the SE losartan group. Then, the rats in the different groups were given the corresponding interventions for 20 days. After the intervention, the depression levels of the rats in each group were evaluated. Upon completion of the intervention, brain tissue was collected for comprehensive assessment of TGF-β mRNA levels, TGF-β/Smad Signaling-related protein expression, and GFAP⁺ astrocyte density in the rat hippocampus. Results The depression-like behavior of SE rats was more obvious than that of control rats. TGF-β mRNA, p-Smad2, p-Smad3 and GFAP⁺ astrocyte expression levels were significantly greater in SE rats than in control rats. Compared with the SE-saline group, the depression-like behavior of the SE-losartan group improved. The expression levels of TGF-β mRNA p-Smad2, p-Smad3 and GFAP⁺ astrocyte expression in the SE-losartan group rats were lower than those in the SE-saline group. Conclusion The TGF-β/Smad Signaling is an important Signaling leading to depression-like behavior in epileptic rats. Losartan is a drug that can significantly improve the depression-like behavior of epileptic rats by regulating the TGF-β/Smad Signaling. Findings in this study support losartan as a repurposable central nervous system drug for comorbid depression in epilepsy, warranting further clinical investigation.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Zeyulin Zhang,Dinghe Liu,Yiru Yan,Liru Zeng,Hao Chen,Qingwen Song,Dazheng Chen,Chunxiang Zhu,Chunfu Zhang,Yuming Zhang,Yue Hao
Gallium oxide (Ga 2 O 3 ) film exhibiting the solar-blind absorption cutoff characteristic holds considerable potential for application in UV photodetectors . The material defects in the device will introduce the recombination region for the photodetector, especially at the interface of the Ga₂O₃ heterojunction . The photogenerated carriers , generated within the Ga 2 O 3 film, will inevitably be influenced by these high recombination centers during their transportation. Avoiding or minimizing this region can improve the performance of the photodetector. The p-n junction, with its depletion region , generates an electric field that drives the carriers away from the recombination region. However, due to the Ga 2 O 3 p-type doping difficulty, our work focuses on the growth of single-crystal NiO/β-Ga₂O₃ films by Mist-CVD and the fabrication of field-controlled photodetectors. The hole concentration in the NiO film is adjusted by doping with different concentrations of Li⁺. Compared with a photodetector without the NiO interlayer, the performance of the photodetector with the structure of NiO (1 % Li⁺-doped)/β-Ga₂O₃ exhibits a significant improvement, which achieved the maximum responsivity of 1002.5 A/W, and a high peak detectivity of 3.31 × 10 15 Jones.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shupeng Su,Junhua Lu,Xinyi Yin,Mengjiao Wang,Fumin Wang,Yuejiu Pang
β-carotene, a vital carotenoid, is limited by poor solubility and stability. Though Pickering nanoemulsions offer superior protection for β-carotene than conventional nanoemulsions, developing food-grade Pickering nanoemulsions remains challenging due to particle size and amphiphilicity issues. This study developed a food-grade Pickering nanoemulsion stabilized by chitosan nanoparticles via high-power ultrasonication. Under optimized conditions (pH 6.5, 2 % oil phase, 1.0 wt% chitosan, 455 W ultrasonication for 180 min), a stable nanoemulsion of 388 nm was obtained with minimized β-carotene loss. These nanoemulsions exhibited exceptional environmental stability, maintaining robust stability under varying pH (3.0–7.0), ionic strength (100–500 mM NaCl), and thermal stress. Thermal stability and extended storage tests demonstrated superior β-carotene protection compared to conventional Tween 20-stabilized emulsions. This work provides a novel strategy for food-grade Pickering nanoemulsions, enabling β-carotene application in functional foods and pharmaceuticals.
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Polymers
·
2023
·Vol. 15
·期 4
·1023
·
IF 5
Jingru Wu,Jing Li,Fang Xu,Arong Zhou,Shaoxiao Zeng,Baodong Zheng,Shaoling Lin
A biodegradable photodynamic antibacterial film (PS-CF) was prepared using the casting method, with κ-Carrageenan (κ-Car) as the film-forming substrate and curcumin-β-cyclodextrin (Cur-β-CD) complex as photosensitizer. Chilled pork samples were coated with PS-CF and stored at 4 °C to investigate the effects of PS-CF combined with LED light irradiation (425 nm, 45 min) (PS+L+) on pork preservation during 10 days of storage. The total viable count (TVC) of bacteria, total volatile basic nitrogen value (TVB-N) and the pH of pork treated with PS+L+ were all lower than the control, and the water-holding capacity (WHC) was higher. Ten days later, the TVB-N value was 12.35 ± 0.57 mg/100 g and the TVC value was 5.78 ± 0.17 log CFU/g, which was within the acceptable range. Sensory evaluation determined that the color, odor, and overall acceptability of pork treated with PS+L+ were significantly better than the control. These findings suggest that PS+L+ treatment effectively extended the shelf life of chilled pork from ~4–5 to 10 days. Correlation analysis showed that the sensory quality of the chilled pork significantly correlated with total bacterial counts, TVB-N and thiobarbituric acid reactive substances (TBARS) (p< 0.05), suggesting that these biomarkers could be used as standard indicators for evaluating the freshness of chilled pork. These findings demonstrate the effectiveness of Cur-β-CD photodynamic antibacterial film for the preservation of chilled pork and provide a theoretical basis for the application of the film for the preservation of fresh food in general.Keywords:curcumin;storage;chilled pork;photodynamic inactivation
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