Frontiers in Nutrition
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2022
·
IF 6.59
Qianqian Yao,Linlin Fan,Nan Zheng,Christophe Blecker,Véronique Delcenserie,Huiying Li,Jiaqi Wang
Gut microbiota dysbiosis, together with goblet cells dysfunction has been observed in ulcerative colitis cases. This study aims to evaluate the potential of 2′-fucosyllactose (2′-FL) supplementation in inhibiting intestinal inflammation through regulating gut microbiota, protecting goblet cells, and stimulating mucin secretion. 2′-FL was orally administered to C57BL/6J mice daily (400 mg/kg bw) for 21 days and 5% dextran sulfate sodium (DSS) was used to induce the colitis in the last 7 days. Meanwhile, fecal microbiota transplantation (FMT) was conducted to test the roles of gut microbiota in the remission of colitis by 2′-FL. Gut microbiota alteration was analyzed through 16S ribosomal RNA (16S rRNA) sequencing. Periodic acid-Schiff (PAS), immunofluorescence staining, as well as mucin 2 (MUC2) and NOD-like receptor family pyrin domain containing 6 (NLRP6) messenger RNA (mRNA) expression in colon fragments was performed and detected. The results showed that the DSS + 2′-FL mice were found to have a slower rate of weight loss, lower disease activity index (DAI) scores, and longer colon lengths than the DSS group ( p < 0.05), so in the FMT recipient mice which received fecal microbiota from the DSS + 2′-FL group. In addition, the data revealed that 2′-FL relieved the disorder of DSS-induced gut microbiota, including decreasing the high abundance of mucin-utilizing bacteria in the DSS group, such as Bacteroides, Lachnospiraceae NK4A136, Lachnospiraceae , and Bacteroides vulgatus . PAS and immunofluorescence staining showed that 2′-FL treatment promoted the recovery of goblet cells and enhanced MUC2 and NLRP6 expression, which was also observed in the FM (DSS + 2′-FL) group. Moreover, NLRP6, which has been proved to be a negative regulator for Toll-like receptor 4/myeloid differential protein-8/nuclear factor-kappa B (TLR4/MyD88/NF-κB) pathway, was upregulated by 2′-FL in colon tissue. In conclusion, this study suggests that 2′-FL ameliorates colitis in a gut microbiota-dependent manner. The underlying protective mechanism associates with the recovery of goblet cells number and improves MUC2 secretion through TLR4-related pathway.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Xiaohui Wang,Xue Wang,Yali Wang,Quanguo Zhai,Yucheng Jiang,Shuni Li
Octahedral molecular sieve – 5 (OMS-5), a typical 2 × 4 tunnel manganese oxide, is considered as an adsorbent for rubidium ion (Rb + ) because of its specific tunnel structure. Also, manganese oxide-based nanomaterials are also widely used in supercapacitor energy storage and capacitor deionization electrodes due to their high specific capacitance. In this work, a series of protonated OMS-5 (H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5) nanobelts are synthesized and used as electrode materials for electrochemical enrichment of Rb + in aqueous solution. The specific capacity values for H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5 at 1 A·g −1 are of 159, 168, and 215 F·g −1 , respectively. Thanks to the unique protonated 2 × 4 tunnel structure, well regulating surface area, and excellent specific capacitance, the maximum Rb + adsorption capacities of H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5 can reach to 390.3, 405.7, and 481.7 mg·g −1 , respectively. These results indicate that the protonated 2 × 4 tunnel manganese dioxide nanobelts are of great potentials as faradaic electrode materials for selective rubidium ion enrichment applications.
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TALANTA
·
2022
·
IF 6.556
Kai Zhang,Zhenqiang Fan,Yue Huang,Yuedi Ding,Minhao Xie,Minghe Wang
In this work, we designed an ECL ratiometric biosensor with a three-stranded Y-type DNA (Y-DNA) probe and induced a hybridization chain reaction (HCR) for the highly sensitive detection of SARS-CoV-2 nucleic acid. The important component of this system is the self-assembled Y-Shaped probe based on three nucleic acids. Y1, Y2, and Y3 can be linked by complementary base pairing to Hairpin1 (H1), Hairpin2 (H2), and Ru modified DNA (Ru1), respectively. H1 and H2 can trigger the HCR reaction when activated by the SARS-CoV-2 RdRp gene and the 5′ end of Ru1. The 5′ end of Ru1 is modified with the Ru complex, which can produce a strong electrochemiluminescence luminescence signal at 620 nm under an applied voltage. Through the amplification of Y-DNA-induced HCR reaction, Ru1 on the electrode surface gradually increased, the ECL signal at 460 nm was gradually quenched, and the signal at 620 nm was steadily generated. The SARS-CoV-2 RdRp gene can be quantified according to the degree of decrease of ECL signal at 460 nm and the increase of ECL signal at 620 nm. Combining the two signal amplification strategies, this ratiometric ECL biosensor can accurately and efficiently detect the target gene with a detection limit of 59 aM.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Feifei Hou,Dexin Feng,Mo Xian,Wei Huang
2-Acetamidophenol (AAP) is an aromatic product with promising activities in agricultural applications and medical research. At present, AAP is synthesized by chemical methods from nonrenewable fossil fuel resources, which cause environmental pollution and the reaction conditions are harsh. In this study, we constructed the artificial biosynthetic pathway of AAP with five different expressed proteins in Escherichia coli for the first time. By introducing the hydrogen peroxide degrading enzyme catalase and improving cell tolerance to toxic intermediates or products, the yield of AAP reached 33.54 mg/L using shaking-flask culture. The best-engineered strain could produce 568.57 mg/L AAP by fed-batch fermentation from glucose and precursor (2-aminophenol, 2-AP) addition. Furthermore, a one-pot whole-cell cascade biocatalytic pathway to AAP and analogues was developed and optimized. This method can efficiently produce 1.8 g/L AAP using the methyl anthranilate hydrolysis product as the substrate. This study provides not only the de novo artificial biosynthetic pathway of AAP in E. coli but also a promising sustainable and efficient strategy to enable the synthesis of AAP on a gram scale.
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ACS Nano
·
2023
·
IF 17.1
Zhibin Zhang,Dongtao Zhou,Xiaowei Luan,Xuyuan Wang,Zhenxing Zhu,Wen Luo,Jingjing Yang,Shaochun Tang,Yujun Song
Type 2 diabetes (T2D) results from the cells’ insulin resistance, and to date, insulin therapy and diabetes medications targeting glycemic management have failed to reverse the increase in T2D prevalence. Restoring liver functions to improve hepatic insulin resistance by reducing oxidative stress is a potential strategy for T2D treatment. Herein, the liver-targeted biodegradable silica nanoshells embedded with platinum nanoparticles (Pt-SiO2) are designed as reactive oxygen species (ROS) nanoscavengers and functional hollow nanocarriers. Then, 2,4-dinitrophenol-methyl ether (DNPME, mitochondrial uncoupler) is loaded inside Pt-SiO2, followed by coating a lipid bilayer (D@Pt-SiO2@L) for long-term effective ROS removal (platinum nanoparticles scavenge overproduced ROS, while DNPME inhibits ROS production) in the liver tissue of T2D models. It is found that D@Pt-SiO2@L reverses elevated oxidative stress, insulin resistance, and impaired glucose consumption in vitro, and significantly improves hepatic steatosis and antioxidant capacity in diabetic mice models induced by a high-fat diet and streptozotocin. Moreover, intravenous administration of D@Pt-SiO2@L indicates therapeutic effects on hyperlipidemia, insulin resistance, hyperglycemia, and diabetic nephropathy, which provides a promising approach for T2D treatment by reversing hepatic insulin resistance through long-term ROS scavenging.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
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IF 4.539
Qun Liu,Kun Xu,Guangxu Hu,Fanming Zeng,Xiao Li,Chun Li,Yu Zhang
MIL-101(Cr)/polyacrylamide/chitosan (named as MIL-101(Cr)/PAM/CSA) composite aerogel with large surface area is synthesized by incorporating MIL-101(Cr) into polyacrylamide/chitosan composite aerogel and used for efficient 2, 4-Dichlorophenoxyacetic acid adsorption. MIL-101(Cr)/PAM/CSA and polyacrylamide/chitosan composite aerogels exhibit good underwater superelastic properties with multi-level network synergistic cross-linking. After introducing MIL-101(Cr) with the mass loading of 30%, the specific surface area of the composite aerogels enlarge from 58.15 m 2 g −1 to 310.94 m 2 g −1 . MIL-101(Cr)/PAM/CSA exhibits high adsorption capacity of 302.45 mg g −1 for 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and stable recycling capability. This work provides a general and effective method to synthesize composite aerogel with underwater superelastic properties, hierarchical pores and large surface area, promising to realize regeneration after compression with reduced solvent usage and costs.
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Materials Today Chemistry
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2021
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IF 8.301
X. Jiao,H. Li,X. Cheng
This article deals with three cellulose-based fluorescent macromolecular sensors by introducing 1,8-naphthalimide fluorophore to cellulose . First, through the etherification reaction of cellulose with BrCH 2 CH 2 NH 2 , –NH 2 group–bearing cellulose CS 1 was obtained. Then, the –NH 2 group reacts with 4-bromo-1,8-naphthalic anhydride to synthesize a naphthalimide cellulose derivative ( CS 2 ). Finally, the recognition group was introduced by substituting Br atoms, and three cellulose fluorescent probes ( CS 3 , CS 4 , and CS 5 ) were obtained eventually. Structure and fluorescence properties of the macromolecular sensors were characterized and confirmed. Fluorescence detection measurements show that these probes can be used as selective and sensitive fluorescent sensors to 2,4,6-trinitrophenol (TNP). The detection limits are 0.52 μM, 0.76 μM, and 0.81 μM, indicating good detection performance. This work provides a new method for the selective detection of TNP and also a method to enlarge the application scope of cellulose.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Di Wu,Jiebing Yang,Zhen Xing,Haobo Han,Tingting Wang,Aijun Zhang,Yan Yang,Quanshun Li
In this study, the conjugation of phenylboronic acid (PBA) to amine-terminated polyamidoamine (PAMAM) was successfully conducted to prepare a tumor-targeted gene carrier PBA-functionalized PAMAM (PPP) for Bcl-2 siRNA delivery, using a heterobifunctional crosslinker NHS-PEG 5k -Mal. The carrier possessed favorable capacity for siRNA condensation and could protect siRNA from the degradation against RNase and serum. The introduction of PBA could facilitate the cellular uptake and further transfection of Bcl-2 siRNA demonstrated by confocal laser scanning microscopy and flow cytometry. Meanwhile, PPP-mediated transfection of Bcl-2 siRNA could significantly inhibit the expression of Bcl-2 gene at both mRNA and protein levels. Furthermore, owing to the knock-down of Bcl-2, PPP/siRNA could significantly inhibit the cell proliferation by inducing the cell apoptosis, and also enhance the antitumor efficiency of doxorubicin by suppressing the resistance of tumor cells to chemotherapeutics. In conclusion, the PPP-mediated Bcl-2 siRNA delivery could potentially be an effective platform for solving the drug resistance and further achieving the combined chemotherapy and gene therapy in tumor treatment.
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CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Yujie Lv,Yifan Jing,Bao Zhang,Yingxue Li,Guanglin Xia,Xuebin Yu,Pengru Huang,Haixiang Huang,Bogu Liu,Jianguang Yuan,Ying Wu
Although Mg(BH 4 ) 2 has been widely studied in the hydrogen storage field for its high hydrogen density, its terrible dehydriding kinetics and cycling features remain impediments to practical applications. Herein, introducing different MXenes (Ti 3 C 2 , Ti 2 C, Nb 2 C) with novel two-dimensional structures as catalysts into Mg(BH 4 ) 2 has been demonstrated to be an efficient approach to boosting the hydrogen storage properties. 30 wt% MXenes in Mg(BH 4 ) 2 causes a considerable reduction in its hydrogen release temperature, remarkable increases in its dehydrogenation rate and capacity, and an enhancement to its reversibility. The Mg(BH 4 ) 2 -30Ti 2 C as the most excellent composite starts to liberate hydrogen from 132 °C, 143 °C lower than that of Mg(BH 4 ) 2 . Furthermore, at 260 °C, the Mg(BH 4 ) 2 -30Ti 2 C composite desorbs 10.2 wt% H 2 , which is much higher than that of Mg(BH 4 ) 2 (1.5 wt%). Theoretical calculations elucidate the weakening of B-H bonds caused by the electron transfer at the Mg(BH 4 ) 2 /MXenes interfaces, and the dehybridization effect between the B and H orbits. X-ray photoelectron spectroscopy (XPS) analyses prove the in-situ formation of multiphase interfaces resulting from the interaction of Mg(BH 4 ) 2 and MXenes during milling and dehydrogenation processes, which could increase electron conduction and facilitate hydrogen diffusion. These factors contribute to the outstanding dehydrogenation properties of Mg(BH 4 ) 2 .
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 23
·16925
·
IF 5.6
Long Liu,Fan Wang,Wei Yang,Hua Yang,Qiong Huang,Chunlin Yang,Wenkai Hui
Pheromone-binding proteins (PBPs) play important roles in binding and transporting sex pheromones. However, thePBPgenes identified in coleopteran insects and their information sensing mechanism are largely unknown.Cyrtotrachelus buqueti(Coleoptera: Curculionidae) is a major insect pest of bamboo plantations. In this study, a novelPBPgene,CbuqPBP2, fromC. buquetiwas functionally characterized.CbuqPBP2was more abundantly expressed in the antennae of both sexes than other body parts, and its expression level was significantly male-biased. Fluorescence competitive binding assays showed that CbuqPBP2 exhibited the strongest binding affinity to dibutyl phthalate (Ki= 6.32 μM), followed by styrene (Ki= 11.37 μM), among twelveC. buquetivolatiles. CbuqPBP2, on the other hand, showed high binding affinity to linalool (Ki= 10.55), the main volatile of host plantNeosinocalamus affinis. Furthermore, molecular docking also demonstrated the strong binding ability of CbuqPBP2 to dibutyl phthalate, styrene, and linalool, with binding energy values of −5.7, −6.6, and −6.0 kcal/mol, respectively, and hydrophobic interactions were the prevailing forces. The knockdown ofCbuqPBP2expression via RNA interference significantly reduced the electroantennography (EAG) responses of male adults to dibutyl phthalate and styrene. In conclusion, these results will be conducive to understanding the olfactory mechanisms ofC. buquetiand promoting the development of novel strategies for controlling this insect pest.Keywords:Cyrtotrachelus buqueti;pheromone-binding proteins;binding assay;molecular docking;RNA interference;electroantennography
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Chemistry-An Asian Journal
·
2023
·Vol. 18
·期 23
·e202300757
·
IF 4.1
Honglei Zhang,Haibo Zhu,Yingying Zhang,Gaowen Ren,Weiwei Fang,Qiangwen Fan,Zongbo Xie
Graphical An efficient nickel-catalysed sulfonylation of readily available boronic acids, K 2 S 2 O 5 and 2-chlorobenzothiazoles has been successfully developed for the synthesis of structurally diverse (hetero)aryl sulfones without additional bases. A redox-neutral nickel-catalysed sulfonylation for arylsulfone synthesis was developed. (Hetero)aryl boronic acids reacted with potassium metabisulfite (K 2 S 2 O 5 ) and readily available 2-chlorothiazoles in the presence of air-stable Ni(OTf) 2 and 4,4-di- tert -butyl bipyridine (dtbpy) as a commercially available ligand to produce the corresponding 2-sulfonylthiazoles in moderate to excellent yields. This practical protocol tolerates a wide range of substrates including boronic acids and 2-chloro(benzo)thiazoles without additional bases, allowing the direct synthesis of functional arylsulfones.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Fujun Li,Zhaoyan Guo,Wenbo Yang,Yumeng Zhou,Yue Ru,Guicun Qi,Dali Gao,Shengyang Tao
In continuous multiphase flow photochemical reactions, the generation of insoluble materials can significantly reduce the efficiency of material and light energy transfer between interfaces due to numerous interfaces within the hybrid system. The problem of solids blocking pipelines further compounds this issue. This study presents the design and construction of an optical-ultrasonic coupled continuous flow reactor to synthesize the insoluble α-bromoketone (4,4′-bis(2-bromoacetyl)biphenyl) is continuously produced in situ for synthesis, eliminating the need for large quantities of toxic bromine. A flowing cooling medium enhanced the ultrasound power transfer and eliminated the thermal effects of the ultrasound and light sources under TR = 2.1 min, T = 20 °C, and a flow cooling medium thickness of 1.5 cm. The reactants were converted to 92.4% and yielded up to 90.2%, significantly better than the batch reactor results. The experimental results show that the product particles are reduced considerably under ultrasound, eliminating the blockage. Free radical capture experiments investigated the stepwise bromination mechanism, kinetic parameters were calculated, and the reaction rate expressions derived from the basic steps were consistent with the kinetic model. These findings provide valuable insights into the reaction process, contributing to a more comprehensive understanding.
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