INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Dongdong Zeng,Yutian Zhang,Xiaojun Ma,Jianing Li,Fen Yin,Dongna Li,Wenxuan Bie
Co-fermentation of multiple substrates has emerged as the most effective method to improve the yield of bioproducts. Herein, sustainable rubberwood enzymatic hydrolysates (RWH) were co -fermented by Aureobasidium pullulans to produce poly(β-L-malic acid) (PMA), and RWH + glucose/xylose was also investigated as co-substrates. Owing to low inhibitor concentration and abundant natural nitrogen source content of RWH, a high PMA yield of 0.45 g/g and a productivity of 0.32 g/L/h were obtained by RWH substrate fermentation. After optimization, PMA yields following the fermentation of RWH + glucose and RWH + xylose reached 59.92 g/L and 53.71 g/L, respectively, which were 52 % and 36 % higher than that after the fermentation of RWH. RWH + glucose more significantly affected the correlation between PMA yield and substrate concentration than RWH + xylose. The results demonstrated that the co-fermentation of RWH co-substrate is a promising method for the synthesis of bioproducts.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Fangxiu Li,Yue Sun,Xiaoxia Sun,Yu Hu,Yanli Wu
Due to the hydrophilicity of furan ring and hydroxyl group, and hydrophobicity of benzene ring and cyclohexyl group, the compounds are easy to form hydrophilic and hydrophobic amphoteric vesicles through self-assembly. The self-assembled vesicles were designed and facilely synthesized using the 2-furanboronic acid and H 8 -BINOL by Suzuki coupling reaction, which could serve as a novel probe with high enantioselectivity for D/L-Phe. In SEM and TEM images, after adding the L-Phe, the vesicle structure of the H 8 -BINOL derivatives changed from spherical to dendritic nanotube structures. On the contrary, after the addition of D-Phe, the spherical vesicle structure changed only slightly, indicating that the material had specific recognition for L-Phe. Moreover, the new fluorescent probes had unique rigid structures and obvious enantioselective recognition for phenylalanine in fluorescence testes. In the methanol solution, after adding the L-Phe, the fluorescence intensity of R - 1 and S - 1 decreased significantly at 368 nm and increased at 306 nm, but D-Phe had no obvious fluorescence effect on the probes, which indicated that the fluorescent probes had a good guiding effect on the recognition of amino acids by chiral compounds.
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2023
·Vol. 30
·期 11
·1-18
·
IF 2.8
Li Xin,Zhan Shiping
Poly (L-lactic acid) (PLLA) is a bio-based biodegradable polymer with minimal immunogenicity, non-toxicity and excellent mechanical properties and has widely used in medical, pharmaceutical, food packaging and other fields. However, linear PLLA has the characteristics of high melting point, strong crystallinity, weak flowability and poor thermal stability, which cannot meet the requirements of various industrial processes and novel pharmaceutical formulations. With its low viscosity, controlled degradation cycle, and excellent melt flowability, star-shaped PLLA can be used in the fields of biocomposites, biomedical devices, drug delivery, and intelligent packaging. Presently, metal catalysts and organic solvents are frequently used in PLLA synthesis industries, and both residues continue to pose a safety risks. In existing studies, the performance of bioenzymes as optimal green catalysts in organic solvents is not satisfactory, and they are predominantly used to synthesise oligomers with low yields and lengthy reaction times. In this study, a four-armed star-shaped poly (L-lactic acid) (4s-PLLA) with a porous structure favourable for drug loading was synthesised using supercritical carbon dioxide (Sc-CO 2 ) as a solvent, lipase as a catalyst, and erythritol as a nucleus. Using Sc-CO 2 as a solvent the reaction activity and stability of the enzyme were increased so that the reaction yield could reach 83% and the reaction time was only 24 h, which provided a possibility for the practical application of the enzymes catalyze green process. Both the random copolymerization product four-armed star-shaped poly(L-lactic-glycolic acid) (4s-PLGA) and the block copolymerization product four-armed star-shaped poly (L-lactic-glycolic acid) (4s-PLLA-PGA) were subsequently synthesized by copolymerization modification, and their porous structures retained and hydrophilicity were enhanced. The response surface analyses were performed about the reaction time, reaction temperature and yield of synthetic reactions by using Design-Expert 12 software and the prediction equations were generated.
✓ 已验证
DOI
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Xin-Yang Jiang,Cai-Wei Wang,Jun Zhang,Pei-Pei Xu,Yi-Ting Xue,Qian Wang
The tea of fig ( Ficus carica L. ) has long been used as a medicine in China due to its excellent effects on hemorrhoids, damp heat diarrhea and other diseases. The present study was designed to evaluate the impacts of different extraction methods, including hot water extraction (HWE), microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), ultrasound assisted enzymatic extraction (UAEE), ultrasound-microwave assisted extraction (UMAE), alkali extraction (AE) and high-speed shearing homogeneous extraction (HSHE), on physicochemical characteristics and bioactivities of polysaccharide from fig leaves (FLPs). Results indicated that there were great differences between the structure and activity of FLPs prepared by seven methods. Among them, FLPs extracted by UAE (FLPs-UAE) showed the strongest antioxidant and hypoglycemic activities due to its higher Mw, total polysaccharide content (60.62±3.06%) and Glucosamine (GlcN) content (7.68%). In addition, FLPs extracted by HWE possessed more total polysaccharide content (62.03±0.70%), total phenolic compounds content (13.55 ± 0.02 %) and GlcN content (5.78%) contributed to its strong antioxidant activity and the inhibition on α-amylase and α-glucosidase, which was second to FLPs-UAE. These finding suggested that UAE and HWE, especially UAE, were good ways to extract FLPs with high antioxidant and anti-diabetic activities, which provide foundations for choosing the suitable extraction strategy to prepare FLPs with desired bioactivities and promoting the application of FLPs in function food industries.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Wenjia Huang,Yong Li,Maoshuang Chen,Jinlin Chen,Jun Liu,Shuangfei Xiang,Feiya Fu,Xiangdong Liu,Ni Li
Cotton fabric plays an important role in the textile industry, but suffers from bacterial proliferation. The incorporation of naturally occurred phenols onto cotton fiber surfaces has the potential to yield powerful antibacterial effects, yet a simple and feasible method for their covalent grafting is currently lacking. In this study, we present a cleaning technique for preparing antibacterial cotton fabrics by using eugenol (Eug) and L-Cysteine (Cys) as finishing agents. The reactive carboxyl group of Cys is utilized to link Cys molecules onto cotton fibers via an esterification, followed by linking Eug molecules to the Cys moieties through thiol-ene click reaction. Simulated reactions validate the chemical mechanism of the thiol-ene click reaction between Eug and Cys, confirming its feasibility for various applications. Experimental results demonstrate that the modified fabrics achieve high bacteriostatic reduction rates (BR) against both Escherichia coli and Staphylococcus aureus . It is inferred that the Eug moieties incorporated onto the cotton fibers furnish phenolic groups, which interact with saccharides and proteins to exert bactericidal effects. The resulting fabrics also display exceptional antibacterial durability, maintaining over 95 % antibacterial activity after enduring durability tests. Meanwhile, the original properties of the fabrics (strength, flexibility, etc.) are minimally affected by the modification process.
✓ 已验证
产品关联
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 11
·1917
·
IF 7
Yutan Chu,Qiongli Bao,Yan Li,Hongyu Sun,Zewei Liu,Jiahao Shi,Yizong Huang
Antimony (Sb) is a hazardous metal element that is potentially toxic and carcinogenic. Melatonin (MT) is an indole compound with antioxidant properties that plays an essential role in plant growth and alleviates heavy metal stresses. Nevertheless, little is known about the effects and mechanisms of exogenous MT action on rice under Sb stress. The aim of this experiment was to explore the mechanism of MT reducing Sb toxicity in rice via hydroponics. The results showed that Sb stress significantly inhibited the growth of rice, including biomass, root parameters, and root viability. Exogenous MT obviously alleviated the inhibition of Sb stress on seedling growth and increased biomass, root parameters, and root viability by 15–55%. MT significantly reduced the total Sb content in rice and the subcellular Sb contents in roots by nearly 20–40% and 12.3–54.2% under Sb stress, respectively. MT significantly decreased the contents of malondialdehyde (MDA, by nearly 50%), ROS (H2O2and O2·−, by nearly 20–30%), and RNS (NO and ONOO−) in roots under Sb stress, thus reducing oxidative stress and cell membrane damage. Furthermore, MT reversed Sb-induced phytotoxicity by increasing the activities of antioxidant enzymes (SOD, POD, CAT, and APX) by nearly 15% to 50% and by regulating the AsA–GSH cycle. In conclusion, this study demonstrates the potential of MT to maintain redox homeostasis and reduce Sb toxicity in rice cells, decreasing the content of Sb in rice and thereby alleviating the inhibition of Sb on rice growth. The results provided a feasible strategy for mitigating Sb toxicity in rice.Keywords:antimony (Sb);melatonin (MT);rice;antioxidant;AsA–GSH cycle
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 37
·25116-25121
·
IF 3.3
Min Kou,Young-Ho Oh,Sungyul Lee,Xianglei Kong
We present the origin of the observed differentiation of lactose and lactulose achieved by complexation with sodiated L-arginine (ArgNa+). We find that the infrared multiphoton dissociation (IRMPD) bands in 3600–3650 and >3650 cm−1 regimes for gas phase lactose and lactulose, respectively, vanish when forming host–guest complexes with ArgNa+. We interpret these differences in the IRMPD spectra by scrutinizing the interactions between the functional groups (guanidium, –CO2−Na+) in ArgNa+ and –OHs in lactose/lactulose. Our calculated structures and infrared spectra of lactose/ArgNa+ and lactulose/ArgNa+ host–guest pairs indicate that the functional groups interact with the low- and high-frequency –OH stretch modes of lactose and lactulose, respectively, in the 3600–3720 cm−1 window.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Peicheng Huang,Weichun Yang,Qingzhu Li,Qi Liao,Mengying Si,Meiqing Shi,Zhihui Yang
In this study, a novel slightly-soluble selenium (Se) fertilizer (SSF) was successfully applied to address the problems of Cd pollution in paddy soil and rice, and Se deficiency in human beings. The pot and field experiments showed that Cd content in the rice grains was reduced by 48.4%–82.89% and Se content was increased nearly by 30-fold comparing the control group. The application of SSF increased the soil pH and significantly reduced the DGT-extracted Cd in the soil. Moreover, DCB-extractable Fe content on the surface of roots was prompt by SSF, which formed a physical barrier, namely iron plaque (IP), to inhibit Cd translocation to the above-ground tissues of the rice plants. The Cd content in the IP was also decreased before the filling period, possibly contributing to the reduction in major Cd accumulation in the rice grains. In addition, the continuous Se increase and Cd reduction in the IP by the SSF gradually exceeded that of water-soluble Se during the three periods of rice plant growth. This suggests that SSF has high potential to be an effective Se fertilizer for inhibiting Cd uptake and enriching Se in rice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yi Hu,Nuzahat Habibul,Yan-Yun Hu,Fan-Li Meng,Guo-Ping Sheng
Ciprofloxacin (CIP), a widely used fluoroquinolone antibiotic, is frequently detected in aqueous environments, and could be assimilated by vegetable plants to possess potential threats to human and animal health through food chains. However, plant uptake of CIP in different chemical speciation has still far from clear now. Thus, the toxicity and uptake of CIP by rice plants were investigated under different solution pH, owing to its contribution to different chemical speciation of CIP. Results display that high pH-driven changes of CIP from cation (CIP + ) to anion (CIP − ) decreased its adsorption and uptake by excised roots and intact plants, respectively. However, CIP concentrations in roots, stems and leaves all exhibited no significant differences with increasing solution pH. Moreover, six intermediates of CIP were detected and two possible transformation pathways were proposed in rice plants, including firstly oxidation and following consecutive cleavage of piperazine ring. After accumulated in plant tissues, CIP significantly inhibited the plant growth, decreased the photosynthetic pigments contents and enhanced the antioxidant enzyme activities in a concentration-dependent manner. Besides, high pH exacerbated the growth inhibition and changed the oxidative damage responses of rice plants to CIP. These findings indicate that the uptake and toxicity of CIP in rice plants were influenced by solution pH-driven changes of its chemical speciation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yufei Yang,Shudong He,Yi Zhang,Xingjiang Li,Haiyan Liu,Qiming Li,Xiaodong Cao,Yongkang Ye,Hanju Sun
Ethanol protocol, two-step protocol and enzymatic extraction were used to extract prolamins from seven kidney beans ( P. vulgaris L.) compared with Osborne protocol. 70–80% (v/v) ethanol concentrations were beneficial to the extraction, and two-step protocol was conducive to higher prolamin content. Varied protein subunits were observed in electrophoresis profiles, and further differences in composition were identified by LC-MS/MS while potential functions were annotated. Stronger hydrophobicity was found with prolamins enrichment from light speckled kidney bean (LSKB), black speckled kidney bean (BSKB), and red kidney bean (RKB), which was negative correlation with α-helix content based on the principal component analysis (PCA). The crude prolamins from the two kinds of white kidney beans possessed higher sulfhydryl and exhibited better water holding capacity (WHC), while higher oil absorption capacity (OAC) was observed in LSKB, BSKB, and RKB. Compared with zein, kidney bean prolamins could be used as food packaging materials due to hydrophobicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2021
·
IF 6.4
Chuan Fu,Yikun Jiang,Xiaoyu Yang,Yu Wang,Wei Ji,Guoliang Jia
Purpose Insufficient biological activity heavily restricts the application and development of biodegradable bone implants. Functional modification of bone implants is critical to improve osseointegration and bone regeneration. Methods In this study, L-lysine functionalized graphene oxide (Lys-g-GO) nanoparticles and polydopamine-assisted gold nanoparticle (AuNPs-PDA) coatings were applied to improve the biological function of PLGA scaffold materials. The effects of Lys-g-GO nanoparticles and AuNPs-PDA functionalized coatings on the physicochemical properties of PLGA scaffolds were detected with scanning electron microscopy (SEM), contact angle measurement, and mechanical testing instruments. In vitro, the effects of composite scaffolds on MC3T3-E1 cell proliferation, adhesion, and osteogenic differentiation were studied. Finally, a radial defect model was used to assess the effect of composite scaffolds on bone defect healing. Results The prepared AuNPs-PDA@PLGA/Lys-g-GO composite scaffolds exhibited excellent mechanical strength, hydrophilicity and antibacterial properties. In vitro, this composite scaffold can significantly improve osteoblast adhesion, proliferation, osteogenic differentiation, calcium deposition, and other cell behaviour. In vivo, this composite scaffold can significantly promote the new bone formation and collagen deposition in the radial defect site and presented good biocompatibility. Conclusion The combination of bioactive nanoparticles and surface coatings shows considerable potential to enhance the osseointegration of bone implants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yungang Cao,Baoling Li,Xin Fan,Jiankang Wang,Zhenbao Zhu,Junrong Huang,Youling L. Xiong
The influence of combined Lysine (Lys) and transglutaminase (TG) on the conformation and gelling properties of oxidatively damaged myofibrillar protein (MP) was investigated. The addition of Lys (5 mM) significantly increased the α-helix content (by 47.8%) and decreased the particle size of oxidatively damaged MP, and improved the cooking yield (by 16.8%) and the breaking strength of MP gels (by 65.5%). The treatment with TG (E:S = 1:500) led to a slightly reduced α-helix content but improved breaking strength (by 41.8%) and cooking loss (by 13.3%) of the gels. Their combination (Lys + TG) showed the greatest and synergistic overall improvement, with the set gel displaying a fine, smooth and compact network structure. Notably, the gelling ability of oxidatively damaged MP upon Lys + TG treatment was significantly stronger than that of non-oxidized MP far exceeding its recovery. Therefore, significantly enhanced gelling properties of oxidatively damaged MP can be attained through the combination Lys and TG.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）