TALANTA
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2021
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IF 6.057
Qiaoge Bayin,Lei Huang,Chunhui Ren,Yusheng Fu,Xing Ma,Jinhong Guo
Since December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused millions of deaths and seriously threatened the safety of human life; indeed, this situation is worsening and many people are infected with the new coronavirus every day. Therefore, it is very important to understand patients’ degree of infection and infection history through antibody testing. Such information is useful also for the government and hospitals to formulate reasonable prevention policies and treatment plans. In this paper, we develop a lateral flow immunoassay (LFIA) method based on superparamagnetic nanoparticles (SMNPs) and a giant magnetoresistance (GMR) sensing system for the simultaneously quantitative detection of anti -SARS-CoV-2 immunoglobulin M (IgM) and G (IgG). A simple and time-effective co-precipitation method was utilized to prepare the SMNPs, which have good dispersibility and magnetic property, with an average diameter of 68 nm. The Internet of Medical Things-supported GMR could transmit medical data to a smartphone through the Bluetooth protocol, making patient information available for medical staff. The proposed GMR system, based on SMNP-supported LFIA, has an outstanding advantage in cost-effectiveness and time-efficiency, and is easy to operate. We believe that the suggested GMR based LFIA system will be very useful for medical staff to analyze and to preserve as a record of infection in COVID-19 patients.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaofeng Tan,Ximei Sun,Yan Li,Yuting Zeng,Jinding Gong,Ziyu Wang,Yuhao An,He Li
Bio-inspired enzyme–inorganic hybrid nanomaterials are extensively employed for biological applications owing to their capability of enhancing enzyme activity and stability compared with free counterpart. They play a vital role in signal output and amplification during bioassay, but usually suffer from deactivation and costing problems, which motivates us to seek a more robust signal generation pattern. The coordination interaction between metal ions and amide groups in enzyme drives the evolution of various morphology and structure. Inspired by this, C-reactive protein (CRP) aptamer, with amide groups in their nucleobases, is introduced as an organic part serving as a linker and biorecognition unit to prepare aptamer-inorganic hybrid nanosheets, and be applied for CRP detection. Unlike using organic enzymes as a signal enhancer, the inorganic part, Mn 3 (PO 4 ) 2 , is exploited for generating and transducing firm current signals, which stem from electrochemical active molybdophosphate precipitates reacted by phosphate and external molybdate. This enzyme-free electrochemical aptasensor based on biomineralized Mn 3 (PO 4 ) 2 /aptamer exhibits an excellent detection performance for CRP. And the reliability is also acceptable compared to the traditional immunoturbidimetric method at the hospital, suggesting our method good feasibility for clinical diagnosis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Huashan Gao,Linlin Ding,Rui Liu,Xinhua Zheng,Xichao Xia,Fuan Wang,Jinxu Qi,Weishuang Tong,Yuanhao Qiu
Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-W was isolated from Anoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 × 10 4 Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were β-1, 4-Manp and α-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes.
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高影响因子
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2021
·Vol. 30
·期 2
·1567-1576
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IF 1.819
Ma Jingling,Jia Xingliang,Ma Mingsheng,Zhang Chenfei,Ren Fengzhang,Wang Guangxin
To limit corrosion and retain activity of magnesium anode, magnesium–air batteries with double solutions: 0.5 M Mg(ClO 4 ) 2 - N , N -dimethylformamide (DMF)/0.6 M NaCl-H 2 O, 0.5 M Mg(ClO 4 ) 2 –acetonitrile (AN)/0.6 M NaCl-H 2 O are prepared. Cathodic and anodic reactions proceed in inorganic and organic solutions separately. Discharge performance, discharge morphology, and corrosion behavior of magnesium anode are researched. Results obtained show that organic electrolytes separate anode from the aqueous electrolyte, thus improving anode utilization rate. Due to 0.6 M NaCl solution used in air cathode side, the batteries show higher discharge voltages. A better discharge performance has been observed in double electrolytes at 1 mA·cm −2 discharge. Results of discharge morphology and electrochemical impedance spectroscopy agree well with discharge performance. The discharge mechanism of magnesium anode in different solutions is also discussed.
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JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2020
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IF 2.366
Wei-Ming Chai,Zi-Yi Yu,Mei-Zhen Lin,Qi-Ming Wei,Shuang Song
In this study, 5-methoxy-2-mercaptobenzimidazole (5-M-2-MB) was confirmed as an efficient tyrosinase inhibitor by methods of enzyme kinetic, fluorescence quenching , ANS-binding, thermodynamics, energy transfer, and molecular docking in combination. The results proved that 5-M-2-MB significantly inhibited the tyrosinase (IC 50 = 60 ± 2 nM) in a reversible and competitive way with the K i value of 80 ± 1 nM. It quenched the intrinsic fluorescence of tyrosinase through a static mechanism, and caused conformational change of the enzyme by increasing the hydrophobic region. Moreover, this compound could bind to tyrosinase and form 5-M-2-MB-tyrosinase complex by hydrogen bond and hydrophobic interaction. The interactions were generated between 5-M-2-MB and specific amino acid residues (Trp-358, Thr-308, Glu-356, and Asp-357) located on the A chain of tyrosinase. Therefore, this study would offer a theoretical foundation for developing the new tyrosinase inhibitor.
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高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2020
·Vol. 35
·期 1
·e6027
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IF 3.14
Zinan Dai,Zehao Yu,Ying Bai,Jiayun Li,Jiajian Peng
A simple method for the hydrosilylation of olefins by using air-stable cobalt catalysts is developed. The catalyst system is composed of simple, cheap, and readily available cobalt(II) salts and well-defined terpyridine derivatives as cocatalysts or ligands, and the hydrosilylation processes can be processed smoothly under mild conditions without either Grignard reagents or NaHBEt 3 as activator.
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高影响因子
GEOMICROBIOLOGY JOURNAL
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2020
·
IF 1.989
Xin Hu,Yue Qiao,Long-Qiang Chen,Jian-Fei Du,Yue-Yue Fu,Si Wu,Lei Huang
The demand to repair areas contaminated with hydrocarbon products has led to the development of new technologies for the treatment of contaminants in an unconventional method, that is, no physical or chemical methods are used. Biosurfactants are amphiphilic biomolecules produced by microorganisms that can be used in environments contaminated by petroleum products due to their unexceptionable tensile properties. Petroleum degrading strain Rhodococcus erythropolis HX-2 was found to be an effective producer of biosurfactants. The resulting biosurfactant (named NK) exhibits high physicochemical properties in terms of surface activity. It is capable of reducing surface tension from 54.99 to 28.89 mN/m and critical micelle concentration (CMC) is 100 mg/L. NK was found to be a substitute for chemically synthesized surfactants because of its higher solubilization efficiency for petroleum and polycyclic aromatic hydrocarbons, superior to SDS, Tween 80, Triton X-100 and Rhamnolipid (a wide used biosurfactant). In addition, it exhibits favorable emulsion stability over a wide range of pH (3–10), temperature (20–100 °C) and salinity ranges (5–20 g/L). It was found that the addition of biosurfactant can improve the efficiency of petroleum degradation, therefore it has potential applications in bioremediation.HighlightsRhodococcus erythropolis HX-2 is an effective petroleum degrading strain.HX-2 is a potential source of biosurfactant production.The biosurfactant NK reduces surface tension and exhibits high emulsification activity.The biosurfactant NK is effective over a wide range of temperatures, pH and salinity.The biosurfactant NK shows high solubilization efficiency for petroleum as well as polycyclic aromatic hydrocarbons.
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Nanomaterials
·
2020
·Vol. 10
·期 2
·281
·
IF 4.324
Hao-Hua Deng,Xiao-Qiong Shi,Paramasivam Balasubramanian,Kai-Yuan Huang,Ying-Ying Xu,Zhong-Nan Huang,Hua-Ping Peng,Wei Chen
This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).Keywords:gold nanocluster;6-aza-2-thio-thymine;protein;fluorescence
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FOOD CHEMISTRY
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2020
·
IF 6.306
Yaqi Tu,Yan Xu,Fazheng Ren,Hao Zhang
The characteristics and antioxidant activity of Maillard reaction products (MRPs) from 2′-fucosyllactose (2′-FL), a human milk oligosaccharide, and α-lactalbumin (α-LA) were investigated. MRPs were prepared by reacting 2′-FL with α-LA at 60 °C and 79% relative humidity for up to 72 h. The absorbance and fluorescence intensity of heated α-LA-2′-FL increased as the heating time increased, while the free amino group content decreased, confirming that 2′-FL reacted with α-LA and produced various MRPs at different incubation stages. Conjugates of 2′-FL and α-LA and aggregation of α-LA were observed by SDS-PAGE. The secondary structure of α-LA did not change significantly after reacting with 2′-FL. In terms of antioxidant activity, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and reducing power of α-LA-2′-FL increased significantly when compared with the protein only sample ( p < 0.05). The findings provide a foundation for the characterization and functional analysis of MRPs in dairy products.
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JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
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2020
·
IF 2.717
Wentao Hu,Xi'an Fan,Zigui Luo,Fan Luo,Guangqiang Li,Jian Wang
In this work, Fe/ZnO soft magnetic composite (SMC) is prepared successfully by sol-gel method combined with a pyrolysis process. Formation mechanism and effect of annealing on magnetic properties for the Fe/ZnO SMCs are studied in detail. The results show that Fe/zinc acetate hydrogel mixture will be decomposed into the Fe/ZnO composites by heating above 340 °C. And ZnO is well coated on the surfaces of Fe particles. The Fe/ZnO composite compact has lower total core loss and better frequency stability of magnetic permeability compared with pure Fe compact. In addition, annealing treatment shows an enhanced effect on the magnetic properties for the Fe/ZnO composite compact such as reduced coercivity and increased permeability. The annealed Fe/ZnO SMCs can keep stable magnetic permeability (26) from 50 Hz to 10 kHz, high magnetic induction (1.65 T) and a relatively low core loss of 91.11 kW/m 3 measured under a magnetic flux density of 30 mT at 10 kHz. Therefore, the Fe/ZnO soft magnetic composites prepared by the above-mentioned method provide a great potential application in electromagnetic conversion equipment.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yunfeng Sun,Shuang Li,Ruipeng Chen,Pian Wu,Jun Liang
The T-2 toxin is generated as a secondary metabolite by various species of Fusarium that are found extensively in barley, wheat, maize, and oats; consequently, the development of methods that can rapidly and efficiently detect this toxin is vital. Herein, we report on a novel and highly sensitive method based on DNA hydrogels and etched gold nanorods (AuNRs) to detect the T-2 toxin. The T-2 toxin aptamer acts as a cross-linking agent for a hydrogel in which horseradish peroxidase (HRP) is uniformly embedded. The target T-2 toxin binds to the aptamer, causing the hydrogel to collapse and release the embedded HRP, which catalyzes the reaction of H 2 O 2 with KI to produce I 2 that etches the AuNRs. The UV spectrum of the AuNRs is blue-shifted following etching; this shift is proportional to the T-2-toxin content. This method offers a low limit of detection (0.87 pg mL −1 ), low limit of quantification (2.90 pg mL −1 ), and wide detection range (0.01–10000 ng mL −1 ). Moreover, coffee, corn, and soybean were assayed using the optimized method. The approach offers good recoveries and relative standard deviations of 93.72–103.07 % and 3.87–7.07 %, respectively. The total assay time was 1.5 h. Thus, the new method is promising as an ultrasensitive assay for detecting various small molecules in complex matrices.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Lingbo Feng,Wei Zhang,Minglan Ge,Yufeng Yi,Jieming Xiong
Excellent extraction ability and easy regeneration of the extractant are highly important for the recovery of acetic acid from water by solvent extraction. In this paper, the extraction capability of octanol isomers and some conventional extractants was investigated. It was found that the extraction capability of 2-octanol was significantly better than that of the traditional extractant isopropyl acetate. The liquid–liquid equilibrium data for the ternary system of water + acetic acid + 2-octanol were measured at different temperatures (293.2, 303.2, and 313.2 K) under 101 kPa. The results showed that the distribution coefficient and selectivity decreased with an increase in acetic acid concentration and the temperature had little impact on the distribution coefficient, suggesting that the extraction could be conducted over a large temperature range. The reliability of the equilibrium data was also verified using the Othmer–Tobias and Hand equations, and the correlation coefficients were all close to 1.0, suggesting that the experimental data was reliable. Furthermore, the NRTL (nonrandom two-liquid) and UNIQUAC (universal quasichemical) models were employed to correlate the experimental data, and the binary interaction parameters of the ternary system were obtained. The results indicated that, for the UNIQUAC model, the root-mean-square deviations between the experimental and simulated data at different temperatures were from 0.63% to 0.94% and those for the NRTL model were all within 0.6%, which indicate that the NRTL model is more suitable for the ternary system of water + acetic acid + 2-octanol.
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