JOURNAL OF LUMINESCENCE
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2019
·
IF 2.961
Yan Hua,Daocheng Hong,Sushu Wan,Yanyu Lei,Mingcai Xie,Weiqing Yang,Yu Du,Yuxi Tian
2D perovskite materials have attracted increasing attention in the last few years due to their optical tunability. However, how to control the structure of these materials is still one of the key challenges. In this work, by using photoluminescence microscopy and XRD characterization, we found that ethanol treatment can induce the reorganization of the crystal structure of 2D perovskite materials. The ethanol molecules can insert into (OA) 2 (MA)Pb 2 I 7 (OA = CH 3 (CH 2 ) 7 NH 3 ; MA = CH 3 NH 3 ) crystal lattice and preferentially dissolve MA ions reducing the MA/OA ratio. During the recrystallization, new (OA) 2 PbI 4 phase forms due to the low MA/OA ratio. On the other hand, adsorption of ethanol can induce the photoluminescence (PL) enhancement by passivation of the surface defects . Consequently, PL intensity increases and decreases as the activation/deactivation of the quenching defects. These results provide a possible way to optimize the crystal structure of 2D perovskite under mild condition.
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CHIRALITY
·
2019
·Vol. 32
·期 2
·231-238
·
IF 1.927
Peng Wu,Mengjie Zhang,Yinjun Zhang,Zhao Wang,Jianyong Zheng
In this study, a novel lipase M5 derived from Aspergillus oryzae WZ007 was prone to exhibit high hydrolytic activity and stereoselectivity towards racemic substrate ( R , S )-ethyl 2-bromoisovalerate. ( R )-ethyl 2-bromoisovalerate was obtained by enzymatic resolution, which is the key chiral intermediate for highly efficient enantiomerically fluvalinate. The results showed that the enzymatic reaction was carried out in 120mM racemic substrate for 3 hours, the enantiomeric excess reached 98.6%, the conversion was 51.7%, and E value above 120. Therefore, the novel lipase M5 has the ability to efficiently produce ( R )-ethyl 2-bromoisovalerate, which greatly reduces the industrial production cost of the highly efficient counterpart of fluvalinate.
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Journal of Cancer Research and Therapeutics
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2020
·Vol. 16
·期 2
·209
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IF 1.326
Yu Haipeng,Zhu Bolan,Yang Wan,Zhang Qian,Wang Wei,Zhai Weichun,Lu Lixia,Zheng Ying,Dang Zheng,Li Bin,Li Chuyi,Liang Bin,Zhang Jiucong,Yu Xiaohui
Objective: To research the effect of matrine on the proliferation and migration of HepG2 cells through extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway. Methods: HepG2 cell was selected and divided into blank control group, experimental group ( matrine 1, 2, and 4 mg/mL), and positive control group (PD98059, ERK1/2 inhibitor). MTT measure was used to detect the effective time and concentration which matrine inhibits HepG2 cells. After 24 h, the effect of effective concentration of matrine on the of morphological changing HepG2 cells was observed. The invasion ability was assayed by transwell method, the expression of ERK1/2 and pERK1/2 were detected through Western blot, and reverse transcription polymerase chain reaction was used to test the expression level of ERK1/2 mRNA. Results: With the increase of matrine concentration, the number of adherent HepG2 cells gradually decreased, the morphologic changes gradually became spherical, some cell morphology was incomplete, and even cell fragments appeared. The proliferation and invasion ability of HepG2 cells decreased. The expression of ERK1/2, pERK1/2, and ERK1/2 mRNA downregulated with the increase of matrine concentration (P < 0.05). Conclusion: Matrine inhibits the proliferation and migration of HepG2 cells by downregulating the ERK1/2 signaling pathway.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Yanhua He,Youwei Yu,Xiaoye Wen,Yan Shi,Jianhu Wu,Zhengping Guan,Meilin Cui,Chunling Xiao
A sensitive, efficient and quencher-free fluorescence aptasensor to detect Ochratoxin A (OTA) based on aptamer , 2-aminopurine (2AP) labeled Oligonucleotide sequence, as well as exonuclease I (Exo I) activity was developed. In which the aptamer specific to OTA was modified into a hairpin structure, and 8 bases at the 3′ ends are exposed (H); also, 2AP is embedded in the oligonucleotide complementary to the 8 bases (2AP-probe).The detection principle based on 2AP-probe could be bonded to its complementary sequence and quenches the fluorescence of 2AP; The aptamer has a stronger affinity for the target than its complementary sequence; Exo I can dissociate single-stranded DNA and has little effect on double-stranded DNA as well as folded DNA. In the absence of OTA, the fluorescence of 2AP is quenched due to the complementary pairing of H and 2AP-probe; in the presence of OTA, H selective binding target is detached from 2AP-probe, and the fluorescence of 2AP is slightly restored. Moreover, when the Exo I is added to the detection system, 2AP-probe is dissociated by the Exo I to release the free 2AP, and the fluorescence of the system is further enhanced thereby realizing the detection of OTA. The detection limit of the aptasensor was low as 0.03 nM with a linear range of 0.5–100 nM. Moreover, the aptasensor has good selectivity and practicability and also has good potential in realizing the detection of toxic and harmful substances in food complex matrices.
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JOURNAL OF BIOTECHNOLOGY
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2019
·
IF 3.163
Xue-Ying Zhang,Xiao-Yang Ou,Yi-Jing Fu,Min-Hua Zong,Ning Li
Catalytic transformation of biomass-derived furans into value-added chemicals and biofuels has received considerable interest recently. In this work, aldehyde dehydrogenases (ALDHs) were identified from Comamonas testosteroni SC1588 for the oxidation of bio-based furans into furan carboxylic acids. Of the whole-cell biocatalysts constructed, Escherichia coli expressing a vanillin dehydrogenase ( E. coli _CtVDH1) proved to be the best for the oxidation of 5-hydroxymethylfurfural (HMF). 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) was obtained in the yield of approximately 92 % within 12 h using this recombinant strain when the HMF concentration was up to 200 mM. In a fed-batch process, 292 mM of HMFCA was produced within 20.5 h, thereby providing a productivity as high as 2.0 g/L h. Other furan carboxylic acids were synthesized in the yields of 83–95%. Besides, the partially purified HMF was smoothly converted into HMFCA by this recombinant strain, with a 90% yield.
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Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 44
·6994-7005
·
IF 5.047
Pengchong Wang,Yan Yan,Ying Sun,Rui Zhang,Chuanchuan Huo,Lu Li,Ke Wang,Yalin Dong,Jianfeng Xing
The transgelin-2 (TAGLN2) protein plays an important role in multidrug resistance in human breast cancer. siRNA mediated gene silencing of TAGLN2 is a promising strategy for paclitaxel resistance reversal in breast cancer. In this study, a series of bioreducible and acid-labile polydiethylenetriamines (PDs) with different proportions of cross-linkers were synthesized. TAGLN2 siRNA was condensed by PDs to form dual-responsive nanocomplexes, and these nanocomplexes were hypothesized to partially degrade in the acidic environment of endosomes, and then completely degrade in the reducing environment of the cytoplasm to release siRNA. It was found that PDs have good water solubility, acid–base buffering capacity, suitable degradability and high biocompatibility. Moreover, PDCKM can deliver TAGLN2 siRNA into MCF-7/PTX cells and inhibit the expression of TAGLN2 even better than PEI 25k. Besides, paclitaxel showed higher cytotoxicity in cells incubated with PDCKM/TAGLN2 siRNA nanocomplexes. These results suggested that PDs have great potential for safe and efficient siRNA delivery to reverse paclitaxel resistance in breast cancer.
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Microorganisms
·
2019
·Vol. 7
·期 10
·379
·
IF 4.167
Huanhuan Shao,Meiju Chen,Xueting Fei,Ronglin Zhang,Yue Zhong,Weimin Ni,Xiang Tao,XinYi He,Erliang Zhang,Bin Yong,Xuemei Tan
A bacterial strain,Streptomyces albogriseolusLBX-2, was isolated from a soil sample in Chengdu, China.S. albogriseolusLBX-2 is an aerobic and Gram-positive microorganism that is capable of using the polyethylene as the sole carbon source. Results of scanning electron microscopy and tensile tests indicated thatS. albogriseolusLBX-2 could cause the damages to polyethylene (PE). Suspension culture of LBX-2 resulted in the weight loss in the PE powder over a 15-day period. The bacterial growth curve assay clearly demonstrated the utilization of n-hexadecane and n-octadecane for the strain LBX-2. Phylogenetic analysis showed that it was grouped in the same clade asS. albogriseolusbelonging toStreptomyces. The complete genome of strain LBX-2 consists of a chromosome of 7,210,477 bp and a linear plasmid of 336,677 bp. Compared with other strains ofStreptomyces, the genome size ofS. albogriseolusLBX-2 was smaller than the average but its guanine and cytosine content (72.47%) was higher than the others. The Non-Redundant Protein Database (NR), Kyoto Encyclopedia of Genes and Genomes (KEGG), SwissProt, Gene Ontology (GO) and Clusters of Orthologous Groups (COG) annotations provided information on the specific functions of encoded proteins. A total of 21 monooxygenase and 22 dioxygenase genes were found in its genome. Synteny comparison with the genome ofStreptomyces coelicolorA3(2) revealed a low overall genetic diversity between them. This study provides valuable information to reveal the underlying mechanisms on PE degradation byS. albogriseolusLBX-2.Keywords:Streptomyces albogriseolusLBX-2;polyethylene biodegradation;genome;synteny comparison
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2022
·
IF 5.168
Yi Yang,Xiangjie Wang,Haolin Zhang,Jiali Li,Jingyi Chen,Miao Yu,Guangxing Li,Ruili Zhang,Ming Ge
Organophosphorus flame retardants (OPFRs) are increasingly being used in many industries since brominated flame retardants (BFRs) have been phase-out. However, OPFRs are associated with environmental pollution and animal health risks, especially in the farming industry. Nevertheless, no study has evaluated the toxicity of OPFRs, as a new flame retardant, on avian species. In order to investigate the specific toxic effects of 2-ethylhexyl diphenyl phosphate (EHDPHP) exposure on chickens and the molecular biological mechanisms that cause damage to the organism, the chicken liver has been studied as a potential target organ for toxic effects. In this study, 7-day-old male chickens were treated with different concentrations of EHDPHP to further investigate the toxicity and mechanisms of OPRs on birds. The samples were taken at 14 d, 28 d, and 42 d for analysis. EHDPHP exposure affected the growth and development of chickens. Furthermore, the microstructural and ultrastructural observations clearly reflected the damage caused by EHDPHP exposure to the livers. The levels of the liver tissue asparate aminotransferase (AST) and alanine aminotransferase (ALT) decreased with increasing gavage dose. In contrast, the levels of oxidative stress in chicken liver and the mRNA expression of related factors increased with increasing gavage dose. In addition, EHDPHP exposure increased liver tissue iron content and affected mRNA expression and protein levels of ferroptosis-related factors in livers. Besides, ferroptosis causes inflammation, thus promoting the synthesis and release of inflammatory factors. This research indicates that EHDPHP can damage chicken livers through oxidative stress and ferroptosis.
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Nanomaterials
·
2022
·Vol. 12
·期 21
·3844
·
IF 5.719
Zhenlan Xu,Qing Tang,Aimei Hong,Lingxiangyu Li
Along with the development of nanotechnology, nanomaterials have been gradually applied to agriculture in recent years, such as Cu(OH)2-nanorods-based nanopesticide, an antibacterial agrochemical with a high efficacy. Nevertheless, knowledge about physical stability of Cu(OH)2nanopesticide in soil solutions is currently scarce, restricting comprehensive understanding of the fate and risk of Cu(OH)2nanopesticide in the soil environment. Herein we investigated aggregation, sedimentation and dissolution of Cu(OH)2nanopesticide in soil solutions extracted from three different soil samples, wherein commercial Cu(OH)2nanopesticide formulation (NPF), as well as its active ingredient (AI) and laboratory-prepared Cu(OH)2nanorods (NR) with similar morphology as AI, were used as model Cu(OH)2nanopesticides. We found that NPF compared to AI showed less extents of aggregation in ultrapure water due to the presence of dispersing agent in NPF. Yet, moderated aggregation and sedimentation were observed for Cu(OH)2nanopesticide irrespective of NPF, AI or NR when soil solutions were used instead of ultrapure water. The sedimentation rate constants of AI and NPF were 0.023 min−1and 0.010 min−1in the ultrapure water, whereas the rate constants of 0.003–0.021 min−1and 0.002–0.007 min−1were observed for AI and NPF in soil solutions, respectively. Besides aggregation and sedimentation, dissolution of Cu(OH)2nanopesticide in soil solutions was highly dependent on soil type, wherein pH and organic matter played important roles in dissolution. Although the final concentrations of dissolved copper (1.08–1.37 mg/L) were comparable among different soil solutions incubating 48 mg/L of AI, NPF or NR for 96 h, a gradual increase followed by an equilibrium was only observed in the soil solution from acidic soil (pH 5.16) with the low content of organic matter (1.20 g/kg). This work would shed light on the fate of Cu(OH)2nanopesticide in the soil environment, which is necessary for risk assessment of the nanomaterials-based agrochemical.Keywords:soil solutions;aggregation;sedimentation;Cu(OH)2nanopesticide;dissolution
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 23
·16925
·
IF 5.6
Long Liu,Fan Wang,Wei Yang,Hua Yang,Qiong Huang,Chunlin Yang,Wenkai Hui
Pheromone-binding proteins (PBPs) play important roles in binding and transporting sex pheromones. However, thePBPgenes identified in coleopteran insects and their information sensing mechanism are largely unknown.Cyrtotrachelus buqueti(Coleoptera: Curculionidae) is a major insect pest of bamboo plantations. In this study, a novelPBPgene,CbuqPBP2, fromC. buquetiwas functionally characterized.CbuqPBP2was more abundantly expressed in the antennae of both sexes than other body parts, and its expression level was significantly male-biased. Fluorescence competitive binding assays showed that CbuqPBP2 exhibited the strongest binding affinity to dibutyl phthalate (Ki= 6.32 μM), followed by styrene (Ki= 11.37 μM), among twelveC. buquetivolatiles. CbuqPBP2, on the other hand, showed high binding affinity to linalool (Ki= 10.55), the main volatile of host plantNeosinocalamus affinis. Furthermore, molecular docking also demonstrated the strong binding ability of CbuqPBP2 to dibutyl phthalate, styrene, and linalool, with binding energy values of −5.7, −6.6, and −6.0 kcal/mol, respectively, and hydrophobic interactions were the prevailing forces. The knockdown ofCbuqPBP2expression via RNA interference significantly reduced the electroantennography (EAG) responses of male adults to dibutyl phthalate and styrene. In conclusion, these results will be conducive to understanding the olfactory mechanisms ofC. buquetiand promoting the development of novel strategies for controlling this insect pest.Keywords:Cyrtotrachelus buqueti;pheromone-binding proteins;binding assay;molecular docking;RNA interference;electroantennography
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yujie Lv,Yifan Jing,Bao Zhang,Yingxue Li,Guanglin Xia,Xuebin Yu,Pengru Huang,Haixiang Huang,Bogu Liu,Jianguang Yuan,Ying Wu
Although Mg(BH 4 ) 2 has been widely studied in the hydrogen storage field for its high hydrogen density, its terrible dehydriding kinetics and cycling features remain impediments to practical applications. Herein, introducing different MXenes (Ti 3 C 2 , Ti 2 C, Nb 2 C) with novel two-dimensional structures as catalysts into Mg(BH 4 ) 2 has been demonstrated to be an efficient approach to boosting the hydrogen storage properties. 30 wt% MXenes in Mg(BH 4 ) 2 causes a considerable reduction in its hydrogen release temperature, remarkable increases in its dehydrogenation rate and capacity, and an enhancement to its reversibility. The Mg(BH 4 ) 2 -30Ti 2 C as the most excellent composite starts to liberate hydrogen from 132 °C, 143 °C lower than that of Mg(BH 4 ) 2 . Furthermore, at 260 °C, the Mg(BH 4 ) 2 -30Ti 2 C composite desorbs 10.2 wt% H 2 , which is much higher than that of Mg(BH 4 ) 2 (1.5 wt%). Theoretical calculations elucidate the weakening of B-H bonds caused by the electron transfer at the Mg(BH 4 ) 2 /MXenes interfaces, and the dehybridization effect between the B and H orbits. X-ray photoelectron spectroscopy (XPS) analyses prove the in-situ formation of multiphase interfaces resulting from the interaction of Mg(BH 4 ) 2 and MXenes during milling and dehydrogenation processes, which could increase electron conduction and facilitate hydrogen diffusion. These factors contribute to the outstanding dehydrogenation properties of Mg(BH 4 ) 2 .
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Chemistry-An Asian Journal
·
2023
·Vol. 18
·期 23
·e202300757
·
IF 4.1
Honglei Zhang,Haibo Zhu,Yingying Zhang,Gaowen Ren,Weiwei Fang,Qiangwen Fan,Zongbo Xie
Graphical An efficient nickel-catalysed sulfonylation of readily available boronic acids, K 2 S 2 O 5 and 2-chlorobenzothiazoles has been successfully developed for the synthesis of structurally diverse (hetero)aryl sulfones without additional bases. A redox-neutral nickel-catalysed sulfonylation for arylsulfone synthesis was developed. (Hetero)aryl boronic acids reacted with potassium metabisulfite (K 2 S 2 O 5 ) and readily available 2-chlorothiazoles in the presence of air-stable Ni(OTf) 2 and 4,4-di- tert -butyl bipyridine (dtbpy) as a commercially available ligand to produce the corresponding 2-sulfonylthiazoles in moderate to excellent yields. This practical protocol tolerates a wide range of substrates including boronic acids and 2-chloro(benzo)thiazoles without additional bases, allowing the direct synthesis of functional arylsulfones.
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危险品化学品经营许可证（带存储）