EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Chaoji Xiong,Kejie Li,Chunzhi Peng,Xingmao Zhang,Kun Liang,Wei Wu,Qian Chen
In this study, the thiophenyl polyamide ( TPPA ) derived from tri(4-aminophenyl) benzene was synthesized for the removal of Hg 2+ from the aqueous solution. The adsorbent was characterized by the Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, scanning electron microscopy, nitrogen adsorption–desorption, thermogravimetric analysis and differential scanning calorimetry. The adsorption capacity of Hg 2+ is 518.7 mg g −1 at a dose of 1.1 g/L of adsorbent. TPPA was shown to have the following advantages: (i) the high selectivity for the excess metal ions Hg 2+ , (ii) the stability in the pH values ranging between 2 and 6, and (iii) the proper recyclability (87 % of Hg 2+ can be removed in 5 adsorption–desorption cycles). Our analysis suggests the models of the pseudo-second-order kinetics and the Langmuir isotherm are suitable for the description of the adsorption of Hg 2+ on the thiophene network polyamide. Our experimental and theoretical results reveal the relationship between the structures and the adsorption capacities of TPPA , identifying the oxygen atom (amide) and the sulphur atom (thiophene) as the most important chemical-reaction sites.
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LANGMUIR
·
2025
·
IF 3.9
Caiwen Pan,Qinghong Shi,Xiaoyan Dong,Linling Yu,Yan Sun
Effective degradation and detoxification of the highly toxic organophosphate pesticide methyl parathion (MP) are important for pollution treatment and sustainable development. Enzymatic hydrolysis of MP by organophosphate hydrolase (OPH) is an effective way. However, hydrolytic product 4-nitrophenol (4-NP) remains environmentally hazardous. A promising strategy is to reduce 4-NP to 4-aminophenol (4-AP), which not only is weakly toxic but also has high application value. To realize the cascade conversion of MP to 4-AP with the dual objectives of detoxification and recycling, we designed a chemoenzymatic catalyst by immobilizing the OPH within a reduction-catalytic nanocomposite (NCu) constructed by generating copper nanoparticles (Cu NPs) in a zirconium-based metal–organic framework, NU-1000. Optimized OPH@NCu exhibited a 239% enhancement in catalytic activity compared to free OPH, attributed to substrate enrichment by NCu and activation of OPH by Cu2+. Moreover, OPH@NCu exhibited an average cascade conversion rate of 11.1 μmol min–1 gcatalyst–1, achieving a complete conversion of 50 μM MP to 4-AP within 9 min by using a low reductant concentration (15 mM NaBH4). Notably, OPH@NCu demonstrated robust stability, retaining >86% of its activity after being stored for 10 days and nearly 80% of its activity over six reuse cycles. These results validate OPH@NCu as an efficient and sustainable platform for the cascaded detoxification and recycling of MP.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Dong Zhang,Ping Yan,Hong Yu,Jing Sun,Shuyun Zhu,Xian-En Zhao
Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag + -enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag + , which can trigger energy transfer from DNA to Tb 3+ more efficiently. In the presence of 4-AP, Ag + can be reduced into Ag 0 owing to its strong reducibility, inducing the decrease of Tb-DNA complexes at 545 nm remarkably. Besides, 4-AP exhibits intrinsic fluorescence at 375 nm under the same conditions. Therefore, ratiometric detection of 4-AP can be achieved using F 375 /F 545 as readout with a detection limit of 0.4 μM. Real water samples have been tsted to evaluate this method in practical application and satisfactory results have been obtained. Furthermore, the addition of 4-AP into Tb-DNA-Ag + system induces a visible color variation from green to bluish violet, enabling visual detection of 4-AP under the assistance of a smartphone.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yuting Li,Xiaotong Li,Jinshun Ye,Zhenzhao Weng,Xiaozhen Liu,Fengyuan Liu,Jingkun Yan,Lin Li
The reactivity of amino acid residues in proteins towards quinones, along with the concentration of quinones, may play a crucial role in determining the specific sites of reaction between quinones and proteins. The reaction efficiencies of 20 different α-carbon amine group blocked amino acids with 4-methylbenzoquinone (4MBQ) were investigated at pH 7.0 by cyclic voltammetry. Among these, Nα-acetyl- l -lysine, Nα-Boc- l -histidine, Nα-acetyl- l -arginine, and Nα-acetyl- l -cysteine exhibited reactivity towards 4MBQ, with the reactivity sequence being: Nα-acetyl- l -cysteine > Nα-acetyl- l -lysine > Nα-acetyl- l -arginine > Nα-Boc- l -histidine. In addition, the reactivity of Nα-acetyl- l -cysteine towards 4MBQ was over 50-fold greater than that of Nα-acetyl- l -lysine, Nα-Boc- l -histidine and Nα-acetyl- l -arginine. Adducts formed from the reaction of 4MBQ with Nα-acetyl- l -lysine and Nα-acetyl- l -arginine were identified as quinone adducts. In contrast, those formed with Nα-acetyl- l -cysteine and Nα-Boc- l -histidine were identified as phenol adducts through UPLC-QTOF-MS/MS analysis. Irrespective of whether the molar ratio of 4MBQ to protein free thiol groups was 1:2 or 2:1, the residues of cysteine, lysine, arginine and histidine in β-lactoglobulin (β-Lg) invariably engaged in the reaction with 4MBQ. This work may provide valuable information for design polyphenol-protein covalent conjugates which can be further applied as functional additives in food industry by choosing protein with different proportion of lysine, histidine, arginine and cysteine residues.
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TRIBOLOGY LETTERS
·
2025
·Vol. 73
·期 4
·1-16
·
IF 3.3
Sheng Dezun,Yu Hongliang,Zhang Xiao,Zhou Xin
Reducing alloy friction to achieve ultra-low friction is a valuable approach to save energy and reduce pollution from oil use, which is a major challenge for researchers. This study introduces a successful method to achieve ultra-low friction in Ti-6Al-4 V using a hydrated lubricant composed of hydroxyethyl cellulose (HEC). And the effects of speed and concentration on lubricating were investigated. It was found that excessive sliding speeds may lead to lubricant detachment and consequent friction increase, indicating that the adsorption ability of HEC needs to be enhanced in future studies. In addition, when the concentration exceeds 5 wt%, wear loss tends to stabilize across tests with different concentrations, while the friction force increases with rising concentrations. Based on these findings, microscopic studies were conducted to investigate the mechanism of friction reduction. Notably, distinct topographic features resembling ‘valleys’ and ‘plateaus’ were identified on the wear scars in a nanoscale scope. The movement of the surfaces induces the hydrated HEC lubricant to flow from the lower valleys to the higher plateaus, suggesting elastohydrodynamic lubrication mechanisms to form robust films. The valleys serve as lubricant reservoirs, while the plateau tops support the lubricant films to prevent contacts between Ti-6Al-4 V and Si 3 N 4 . The schematic illustrations depict the microscopic mechanisms for achieving of ultra-low friction on Ti-6Al-4 V alloy.
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SYNTHESIS-STUTTGART
·
2013
·Vol. 45
·期 24
·3349-3354
·
IF 2.5
Haidong Wei,Tianbin Li,Yue Zhou,Lihong Zhou,Qingle Zeng
Alkyl halides, including heteroarylmethyl and arylmethyl halides, bromoacetate, and cinnamyl bromide, are readily prepared via halogenation from basic raw materials, even using green processes. It is essential to replace their downstream products with them to prepare medicinally important heterocycles quinazolin-4(3H)-ones. Copper(I) bromide catalyzed domino reaction of alkyl halides and anthranilamides under air affords 2-substituted quinazolin-4(3H)-ones in good to excellent yields and with wide functional group tolerance. A mechanism for a copper(I) bromide involved organometal-catalyzed reaction via a four-step domino reaction is proposed.
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TALANTA
·
2021
·
IF 6.057
Lichen Gou,Xiaoliang Zeng,Huan Du,Ling Li,Yunfei Tian,Xiandeng Hou,Li Wu
Herein, a facile and fast surface-enhanced Raman scattering (SERS) method with ratiometric strategy was developed for detection of 4-methylimidazole (4-MI). Via a chemical derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, 4-MI could be converted to SERS-sensitive species. The SERS intensity ratio between the peaks at 1243 cm −1 and 1110 cm −1 (I 1243 /I 1110 ) was used for the quantification of 4-MI. In addition, the method sensitivity was further improved by the aggregation of beta-cyclodextrin-modified Ag nanoparticles (beta-CD-AgNPs). Under the optimal conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for 4-MI were 1.7 nM (S/N = 3) and 5.7 nM (S/N = 10), respectively. The relative standard deviation (RSD) for 0.5 μM 4-MI was 8.2% (n = 20). This method was successfully used for the determination of 4-MI in cola samples with recoveries ranging from 92% to 106%. The present method is convenient, sensitive, selective, reliable and may have a promising application in determination of the compounds with an imidazole ring containing active hydrogen atoms .
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FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Xin-Yun Wang,Jing Xie,Xin-Jun Chen
This study aimed to investigate the lipid oxidation and distribution in Bigeye tuna stored at 0 °C and 4 °C for 6 days. Tuna were evaluated by determining the peroxide value (POV), acid value (AV), anisidine value (AnV), polyene index, fluorescence ratio (FR), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) content, and major glycerophospholipid molecular species. The value of lipid oxidation indexes (POV, AV, AnV, FR, PC, PE and PI) increased as the storage time increased. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) results indicated that the major types of lipids included diacylglycerol (DAG), monoacylglycerol (MAG), phospholipid (PL), and triacylglycerol (TAG). At least 136 PC and 64 PE molecular species were identified in Bigeye tuna. The results of the confocal laser scanning microscope analysis indicated the distribution of TAG and PL particles. In addition, principal component analysis showed that the contents of PI and TAG were positively correlated with PC, polyene index and lipid content but negatively correlated with PI, POV, FR, AOV, AnV, MAG, and DAG, which might be explained by distinguishing the lipid parameters affecting lipid oxidation. Therefore, this study may provide a novel method to evaluate lipid changes and contribute to the balanced nutritional value of aquatic foods during cold storage.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2013
·
IF 3.458
Xiaomin Yang,Lin Zhao,Laszlo Almasy,Vasil M. Garamus,Aihua Zou,Regine Willumeit,Saijun Fan
Chemically modified tetracyclines (CMTs) have been reported to strongly inhibit proliferation and metastasis of various cancers, but their efficacy is restricted by poor water solubility. In the present study, a hydrophilic 4-dedimethylamino sancycline (CMT-3) loaded nanostructured lipid carrier (CMT-3/NLC) was produced by high pressure homogenization (HPH). The physical properties of CMT-3/NLC formulations were characterized by dynamic light scattering (DLS), high efficiency liquid chromatography (HPLC), atomic force microscopy (AFM), scanning electron microscopy (SEM), small-angle neutron scattering (SANS), small-angle X-ray scattering (SAXS) and wide-angle X-ray powder diffraction (XRD). The lipid and surfactant ingredients, as well as drug/lipid concentrations (m/m) were optimized to produce stable and sustained NLC formulations. In vitro cytotoxicity of CMT-3/NLC against HeLa cells was evaluated by MTT assay. The diameter of CMT-3/NLC was found to increase from 153.1 ± 3.0 nm to a maximum of 168.5 ± 2.0 nm after 30 days of storage, while the entrapment efficiency remained constant at >90%. CMT-3/NLC demonstrated a burst-sustained release profile in release media with different pH, a property attributed to the 3-dimensional structure of CMT-3/NLC. Cell uptake and localization studies indicated that NLC reached the cytoplasm and could thereby facilitate CMT-3 entry into HeLa cells.
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PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
Mujiao Zhang,Peiyuan Yao,Shanshan Yu,Tongcun Zhang,Qiaqing Wu,Dunming Zhu
An efficient method for the production of 4-cyanobenzoic acid from terephthalonitrile was developed by using a novel nitrilase from Pantoea sp. AS-PWVM4 (PaNIT). Under the optimized reaction conditions (pH 8.0 and 37 °C), up to 100 g/L substrate concentration was almost completely converted to 4-cyanobenzoic acid with wet whole cells loading of 5.0 wt% in 4 h and the isolated yield was 91%. The substrate concentration, yield and productivity (26.13 g/(L h)) are much higher than those in the previous studies. Furthermore, PaNIT has broad substrate specificity of catalyzing the hydrolysis of various aromatic nitriles and aliphatic nitriles. These results suggest that PaNIT is a valuable nitrilase with potential practical applications.
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Anti-Cancer Agents in Medicinal Chemistry
·
2022
·Vol. 22
·期 17
·3009-3024
·
IF 2.527
Zhu Yuting,Wang Huai,Yu Mingjun,Li Chao,Meng Xiaoming,He Meng,Yao Risheng
Background: GRPR is over-expressed in cancer cells and is a potential drug target for the treatment of cancer. PD176252, as the most representative non-peptide inhibitor of GRPR, can inhibit the growth of cancer cells, but its low selectivity to cancer cells and normal cells limits
its further application. Objective: The aim of this study was to design and synthesize novel GRPR inhibitor with stronger anti-cancer activity and higher affinity with GRPR than the lead compound PD176252. Methods: A series of 1, 3, 4-oxadiazole derivatives as PD176252 analogues
(4a-4j, 6a-6q) were synthesized and their cytotoxic activity was investigated on four cancer lines with high expression of GRPR (gastric (HGC-27), colon (HCT- 116), prostate (PC-3), and lung (A549)) and one human cell line (gastric mucosal epithelial (GES-1)) by MTT assay. Flow cytometry analysis
and Western Blot were used to determine whether the compound induced programmed apoptosis of cancer cells. Competitive binding experiment was used to verify the affinity between GRPR and the optimal compound. Results: Compound 6m exhibited significant growth inhibition on all tested cancer
cell lines, especially gastric cancer cells (HGC-27 cellular IC50 0.37 ± 0.04μM). Also, the selectivity of 6m to HGC-27 was much higher than that of PD176252. Flow cytometric analysis and Western Blot proved that 6m significantly promoted the apoptosis of HGC-
27 cells. Moreover, competitive binding experiment confirmed the close binding of 6m with GRPR, which indicated 6m with a higher affinity than lead compound PD176252. Conclusion: Our results suggested that 6m, as a novel GRPR inhibitor, had a higher affinity with GRPR and potential anti-cancer
effect than PD176252, which can be used as a template for further optimization.
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ACS Biomaterials Science & Engineering
·
2021
·
IF 4.749
Wenhao Wang,Yuehua Liu,Zifan Liu,Shuai Li,Changxu Deng,Xiaoxiao Yang,Qian Deng,Yeye Sun,Yuxin Zhang,Zhenjiang Ma,Wentao Li,Yang Liu,Xiaojun Zhou,Tao Li,Junfeng Zhu,Jinwu Wang,Kerong Dai
Defects in the formation of microvascular networks, which provide oxygen and nutrients to cells, are the main reason for the engraftment failure of clinically applicable engineered tissues. Inflammatory responses and immunomodulation can promote the vascularization of the engineered tissues. We developed a capillary construct composed of a gelatin methacrylate-based cell-laden hydrogel framework complexed with interleukin-4 (IL-4)-loaded alginate-chitosan (AC) microspheres and endothelial progenitor cells (EPCs) and RAW264.7 macrophages as model cells. The AC microspheres maintained and guided the EPCs through electrostatic adhesion, facilitating the formation of microvascular networks. The IL-4-loaded microspheres promoted the polarization of the macrophages into the M2 type, leading to a reduction in pro-inflammatory factors and enhancement of the vascularization. Hematoxylin and eosin staining and immunohistochemical analysis revealed that, without IL-4 or AC microspheres, the scaffold was less effective in angiogenesis. We provide an alternative and promising approach for constructing vascularized tissues.
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