Bioresource Technology Reports
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2022
·
IF 0
Jiali Mi,Simin Liu,Yan Du,Haishan Qi,Lei Zhang
Cadaverine (1,5-diaminopentane) has widespread applications in industry, agriculture and medicine. Cadaverine can be biotransformed from l -lysine by lysine decarboxylasey which is a pyridoxal 5′-phosphate (PLP)-dependent enzyme. Herein, barium alginate microspheres were fabricated by electro-dripping and employed as immobilized metal ion affinity chromatography matrix to purify and immobilize engineered lysine decarboxylase CadA-EK. Besides, polyethyleneimine (PEI) was employed for co-immobilizing PLP and enzymes (cCadA-EK-PEI/PLP). The cCadA-EK-PEI/PLP can produced 184.53 g/L cadaverine with yield of 90.03% in 2 mol/L lysine solution. And the stability of co-immobilized CadA-EK was 1–4 times higher than that of iCadA-EK + PLP in environmental factors, and the catalytic efficiency k cat / K m was increased by 3.4-fold. Furthermore, a continuous flow microreactor with the co-immobilized enzymes and cofactors was developed to catalyze lysine, with 14.28 g/(L·h) productivity of cadaverine, which displayed great potential for cadaverine biosynthesis. This work provides alternative strategy for the one-step purification and co-immobilization of enzyme and cofactor.
研究领域:
生物催化
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酶工程
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生物合成
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材料科学
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DOI
Energy Sources Part A-Recovery Utilization and Environmental Effects
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2018
·
IF 0.555
Hongguo Wu,Yancai Liu,Hu Li,Song Yang
A series of low-cost and sustainable amino-acid derived catalysts including glutamic acid hydrochlorate (GluCl), glycine hydrochlorate (GlyCl), alanine hydrochlorate (AlaCl) and valine hydrochlorate (ValCl) were prepared by using a simple assembly method and characterized by 1H/13C NMR (nuclear magnetic resonance) and FT-IR (Fourier transform infrared) techniques. These amino-acid based catalysts were highly efficient for the production of an important platform molecule 5-hydroxymethylfurfural (HMF) from biomass-derived sugars under oil-heating conditions. A high HMF yield of 83.7% at fructose conversion of 99.3% was achieved in merely 15 min at 120ºC. The catalyst could be reused for four times with no significant loss of its catalytic activity.
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DOI
高影响因子
Desalination and Water Treatment
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2024
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IF 1
Pingping Cheng,Fangyuan Sun,Wei Wang,Jingwei Feng,Zhen-Hu Hu,Shoujun Yuan,Qiquan Wang
5-Fluorouracil (5-FU) and cytarabine (Ara-C) are two commonly used antineoplastic drugs, which are ecotoxic and genotoxic and frequently detected in hospital wastewater. Potassium permanganate (KMnO 4 ) has been widely used to remove organic pollutants from wastewater. The oxidative deg- radation of 5-FU and Ara-C in aqueous solution by KMnO 4 has not been reported. In this study, the oxidative degradation of 5-FU and Ara-C with KMnO 4 in aqueous solution was investigated. 5-FU was oxidized rapidly by KMnO 4 due to the olefinic group in the central heterocyclic ring. The degradation kinetics of 5-FU was well described by the equation of –d[5-FU]/dt = k app [5-FU] t 0.77 [KMnO 4 ] t 0.86 . The degradation kinetics of Ara-C followed a generalized second-order rate law. The apparent rate constant (k app ) of these two target compounds was strongly depended on the reaction temperature, pH, humic acid (HA) and water quality. The apparent activation energies (E a ) of 5-FU and Ara-C were 32 and 40 kJ·mol –1 , respectively, indicating that 5-FU was more easily decomposed by KMnO 4 as compared with Ara-C. The maximum values of k app for 5-FU and Ara-C were 1.73 × 10 –4 μM –0.63 ·s –1 at pH 7.4 and 1.52 M –1 ·s –1 at pH 3.5, respectively. The oxidative degradation of 5-FU was inhibited by HA, while the oxidation of Ara-C was enhanced by HA. Reaction of both 5-FU and Ara-C was influenced by water quality. Thus, this study provides new insights into the degradation of antineoplastic drugs.
研究领域:
环境化学
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药物污染
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水处理
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降解动力学
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抗肿瘤药物
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DOI
高影响因子
ACS Applied Energy Materials
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2018
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IF 0
Lin Long,Zhenzhong Lan,Zhixiong Han,Yunfeng Qiu,Weixing Zhou
Endothermic hydrocarbon fuel has many advantages as a cooling medium in hypersonic aircraft; however substantial application has been greatly hindered due to easy coking, uncontrollable gas products, and low heat sink issues. Catalytic cracking of hydrocarbon fuel has been regarded as one of the most effective ways to improve the heat sink via modulating the cracking pathway. The incipient-wetness impregnation method was selected to load Co salts in commercial ZSM-5 in a large-scale manner; subsequent calcination in Ar gas resulted in Co3O4 nanosheet wrapped HZSM-5 composites. Salinization treatment was adopted to facilitate the dispersion of catalyst in a nonpolar solvent. The ratio of Brönsted/Lewis acidity decreased from 8.00 to 2.79 after modification by Co3O4 nanosheets. The synergistically catalytic effect between Co3O4 nanosheets and ZSM-5 was beneficial to the generation of a larger gas production rate with higher content of alkene, and thus resulting in a higher heat sink than benchmarked fuels. Catalytic cracking of n-decane (C10) in the presence of 0.1 wt % Co3O4 nanosheets@ZSM-5 could yield a heat sink as high as 4.64 MJ/kg at 758 °C, much higher than those of bare ZSM-5 (2.99 MJ/kg at 687 °C) and thermal cracking of C10 (3.77 MJ/kg at 728 °C). Meanwhile, the smart combination of Co3O4 nanosheets and commercial ZSM-5 could effectively suppress the coke deposition on the external surface of composites, thus resulting in efficient catalytic cracking at elevated temperatures for obtaining a higher heat sink.
研究领域:
催化化学
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能源材料
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化学工程
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石油炼制
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航空航天推进
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DOI
Research
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2021
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IF 0
Zhuanghe Ren,Xin Zhang,Hai-Wen Li,Zhenguo Huang,Jianjiang Hu,Mingxia Gao,Hongge Pan,Yongfeng Liu
Sodium alanate (NaAlH 4 ) with 5.6 wt% of hydrogen capacity suffers seriously from the sluggish kinetics for reversible hydrogen storage. Ti-based dopants such as TiCl 4 , TiCl 3 , TiF 3 , and TiO 2 are prominent in enhancing the dehydrogenation kinetics and hence reducing the operation temperature. The tradeoff, however, is a considerable decrease of the reversible hydrogen capacity, which largely lowers the practical value of NaAlH 4 . Here, we successfully synthesized a new Ti-dopant, i.e., TiH 2 as nanoplates with ~50 nm in lateral size and ~15 nm in thickness by an ultrasound-driven metathesis reaction between TiCl 4 and LiH in THF with graphene as supports (denoted as NP-TiH 2 @G). Doping of 7 wt% NP-TiH 2 @G enables a full dehydrogenation of NaAlH 4 at 80°C and rehydrogenation at 30°C under 100 atm H 2 with a reversible hydrogen capacity of 5 wt%, superior to all literature results reported so far. This indicates that nanostructured TiH 2 is much more effective than Ti-dopants in improving the hydrogen storage performance of NaAlH 4 . Our finding not only pushes the practical application of NaAlH 4 forward greatly but also opens up new opportunities to tailor the kinetics with the minimal capacity loss.
研究领域:
材料科学
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氢气储存
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纳米技术
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能量存储
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DOI
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2016
·Vol. 44
·期 8
·1286-1290
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IF 0.566
Li-Hua DENG,Jin-Bo DAI,Zhen-Lin XU,Jin-Yi YANG,Hong WANG,Zhi-Li XIAO,Hong-Tao LEI,Yuan-Ming SUN,Yu-Dong SHEN
To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, a Eu 3+ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The experimental conditions were optimized as follows: the AMOZA-OVA concentration was 0.25 μg mL −1 ; the antibody was diluted by 50000 folds, and the competitive reaction time was 50 min. Under the optimal conditions, the method showed a detection limit of 0.01 ng mL −1 , an IC 50 of 0.26 ng mL −1 and a linear range (IC 20 −IC 80 ) of 0.025−2.83 ng mL −1 . The spiked recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suitable for the determination of AMOZ residue in food samples.
研究领域:
食品安全
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分析化学
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免疫分析
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兽药残留
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DOI
高影响因子
Nature Communications
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2026
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IF 15.7
Lai Dingbo,Yu Jia,Ma Zi-Ang,Ye Xiaolin,Wang Chaoxi,Zhang Junqing,Wang Tuo,Zhao Zhi-Jian,Zhang Peng,Gong Jinlong
The electrochemical C–N coupling of biomass-derived 5-hydroxymethylfurfural (HMF) with methylamine offers a promising sustainable route to value-added amines. However, achieving high selectivity remains challenging due to competing side reactions, including C = O hydrogenation and C–C dimerization. This paper describes tailoring the surface structure of Ag catalysts to modulate HMF adsorption and intermediate hydrogenation behavior and thus enhancing C–N coupling selectivity. Ag nanoparticles, predominantly exposing the (111) facet, exhibit higher selectivity compared with Ag nanocubes mainly enclosed by (100) planes. The Ag(111) facet favors the C–N coupling pathway by regulating both adsorption geometry and hydrogenation ability. In-situ Raman spectroscopy and density functional theory (DFT) calculations reveal that an η 1 (C)-aldehyde adsorption configuration, dominant on Ag(111), enhances carbonyl electrophilicity and facilitates C = O polarization, promoting nucleophilic attack by methylamine. In contrast, the η 2 (C,O)–aldehyde configuration, more common on Ag (100), stabilizes the C = O bond, limiting its reactivity. Moreover, Ag(111) shows enhanced imine hydrogenation activity, further improving amine selectivity. This study highlights the significance of facet-dependent adsorption modulation in steering reaction pathways and advancing sustainable electrochemical synthesis.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2026
·
IF 5.4
Meiyu Wan,Tiexin Sun,Yuying Diao,Saijin Huang,Yingying Tong,Ying Liu,Weijie Liu,Meijuan Liu,Shu Jiang,Erxin Shang,Jinao Duan
Ethnopharmacological relevance Dachengqi decoction (DCQ), a well-known Traditional Chinese Medicine (TCM) formula, has been widely applied for anti-constipation in clinical practice for thousands of years. Nevertheless, its pharmacological mechanisms remain not fully elucidated. Aim of the study This research employed a comprehensive approach integrating network pharmacology and metabolomics, supplemented with molecular docking techniques and both in vitro and in vivo experimental validation, to systematically elucidate the bioactive constituents, critical targets, and underlying mechanisms of DCQ in alleviating constipation. Materials and methods This investigation utilized a comprehensive pharmacology integration strategy to forecast the principal therapeutic pathways through which DCQ alleviates constipation. The establishment of a loperamide-induced constipation model in rats served to evaluate the interventive effects of orally administered DCQ. In addition, ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) was used to detect the changes of metabolites in plasma and urine of rats, and the metabolic related targets were analyzed. Combined with network pharmacology, the potential mechanism of DCQ in ameliorating constipation was revealed and further verified by molecular docking. According to the results of joint analysis, the intervention of DCQ mainly involved the serotonin (5-HT) pathway, including the inflammatory pathways of arachidonic acid and cAMP downstream of 5-HT. Therefore, the mRNA and protein expression levels of key factors associated with the 5-HT pathway, including phospholipaseA2 (PLA2), cAMP/PKA/AQPs, and PANoptosis, were detected. Furthermore, an inflammatory model of Caco-2 cells was induced to investigate the effect of DCQ on the 5-HT pathway. The expression of related targets (PLA2, PKA and ZBP1) was detected by reverse transcription quantitative PCR and immunofluorescence to further verify the molecular mechanism of DCQ. Results DCQ demonstrated efficacy in ameliorating constipation symptoms induced by loperamide in rats and reducing inflammatory responses in Caco-2 cells, while normalizing aberrant plasma and urinary metabolic profiles and regulating arachidonic acid metabolism in constipated rats. Integrated metabolomics and network pharmacology analyses revealed that DCQ mainly alleviated constipation via the 5-HT pathway, markedly suppressing the PLA2-mediated inflammation and PANoptosis while concurrently activating the cAMP-PKA-AQPs cascade to notably increase the expression of aquaporins (AQPs). Conclusions DCQ could restore the homeostasis of metabolic profiles in plasma and urine in model rats, activate the 5-HT pathway to alleviate inflammation, and may be involved in the regulation of PANoptosis-related mechanisms in intestinal epithelial cells, providing a new perspective for constipation treatment.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Akkammagari Putta Rangappa,Wei Xiong,Wenhua Xue,Xinxin Lu,Yiqi Geng,Yuchao Shao,Guohan Sun,Jun Zhao
Activation of molecular O 2 on catalytic surfaces to form reactive oxygen species (ROS) is a pivotal step in catalytic reactions. In this study, we synthesized a bimetallic Zn 3 In 2 S₆ (ZIS) catalyst enriched with Pd S interfaces via a straightforward cation exchange process between Pd 2+ and Zn 2+ . These interfaces function as electron reservoirs, and Pd incorporation significantly enhances photocatalytic performance by reorganizing the electronic structure of the ZIS matrix, thereby improving charge carrier dynamics and reaction kinetics. Additionally, Pd modification optimizes O 2 adsorption strength, preventing excessive population of the π* antibonding orbital that can lead to O O bond cleavage and reduce ROS generation. Theoretical calculations reveal that monodentate adsorption of O 2 on Pd-ZIS surfaces promotes efficient photo induced electron transfer across all excited states, enabling effective ROS activation. The combination of experimental findings and theoretical analysis identified the mechanisms underlying the improved selectivity of Pd-modified ZIS catalysts. As a result, the optimized Pd-ZIS-5 photocatalyst demonstrates a high photo-oxidation performance for HMF oxidation, achieving 92.4 % conversion, 86.3 % yield, and 93.4 % selectivity–representing a 1.7-fold improvement over prismatic ZIS. Overall, this work demonstrates how Pd doping and tailored O 2 adsorption strength enhance photocatalytic processes, offering valuable insights for advanced biomass conversion and sustainable chemical production.
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2014
·Vol. 38
·期 8
·3673-3677
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IF 3.159
Xi Juan Zhao,Cheng Zhi Huang
A one step synthesis of water-soluble luminescent copper nanoclusters (CuNCs) reduced and protected by histidine is reported. When excited at 350 nm, the as-synthesized CuNCs exhibit luminescence centered at 456 nm with a quantum yield of 1.6%, which is different from that of histidine alone under the same experimental conditions. Upon addition of guanosine 5′-triphosphate (GTP), the emission of CuNCs is gradually quenched. While other nucleoside triphosphates including ATP, CTP and UTP as well as some inorganic anions such as P2O74−, PO43−, and CH3COO− have no such quenching effect, which indicates that the nucleobase instead of the phosphate anion plays an important role in the emission quenching of CuNCs. The most efficient quenching of the CuNCs induced by GTP can be ascribed to the most reducing ability of guanine among the other nucleobases. This interesting experimental result demonstrates that the as-synthesized CuNCs can be applied to sensing of GTP.
研究领域:
纳米材料
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分析化学
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生物传感器
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纳米技术
✓ 已验证
DOI
高影响因子
Carbon Resources Conversion
·
2026
·
IF 10.9
Zhixing Li,Fei Wang,Yanchao Liu,Jinghong Ma,Kai Bian,Xinwei Zhang,Yanze Du,Ruifeng Li
The efficient conversion of C 9 heavy aromatics into high-value benzene, toluene, and xylenes (BTX) products is essential for optimizing petroleum refining. This process often depends on both diffusion and the unresolved relationship among structure, acidity, and reactivity of zeolite catalysts. This study focuses on the intracrystalline micro-/ meso -pores in ZSM-5 and decouples the interdependent effects of crystalline morphology and Al distribution of Brønsted acid sites (BAS) in ZSM-5 on C 9 aromatic conversion pathways. The hierarchical ZSM-5 catalysts have the identical SiO 2 /Al 2 O 3 ratios (SAR) and the different distribution of framework Al-species. This dominates the acid distribution of the zeolites and catalytic performances of the catalysts in C 9 aromatic reaction. In toluene/trimethylbenzene (TMB) transalkylation, the hierarchical porosity coupled with reduced crystallite size of the ZSM-5 enhances 1,2,4-TMB conversion by 24.4% and xylene selectivity by 17.9% versus conventional microporous ZSM-5. The product distribution is positively correlated to the concentration of Al atoms localized at channel intersections of the ZSM-5. Diffusion-decoupled experiments demonstrated acid site spatial configuration in the ZSM-5 governs the reaction pathway selection, with aluminum enrichment at channel intersections favoring transalkylation over disproportionation. The hierarchical ZSM-5 in submicron with intracrystalline mesopores and enriched intersection-channel aluminum favors transalkylation of C 9 aromatics to BTX. The hierarchical ZSM-5, with submicron crystallites, intracrystalline mesopores, and enriched intersection-channel aluminum, enhances transalkylation of C 9 aromatics to BTX.
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产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2026
·
IF 5.4
Huidong Fang,Shiai Xu,Siyu Han,Bin Zhao,Chao Zhang,Wanji Zhou
T-shaped droplet microfluidic device was used for preparing 5-Fu-loaded chitosan microspheres. • 5-Fu-loaded chitosan microspheres exhibit pH-responsive release patterns and sustainable release characteristics for up to 60 days. • The average particle size of the microspheres is 3.90 ± 0.57 μm, and the particle size distribution is narrow. • Drug-loaded microspheres of different particle sizes have different drug release rates and cycles. Microsphere formulations are a promising drug delivery system for targeted response and prolonged sustained release. However, conventional emulsification crosslinking methods for microsphere preparation usually result in a broad particle size distribution, large particle size, poor uniformity, and consequently erratic drug release kinetics. In this investigation, chitosan microspheres encapsulating 5-Fluorouracil (5-Fu CS-MS) were fabricated using a T-type droplet microfluidic device, and the formulation was optimized by single-factor experiments. The resulting microspheres had a consistent drug release profile, a smooth surface, high monodispersity, and a uniform particle size distribution with an average particle size of 3.90 ± 0.57 μm. The polymer-drug interaction in 5-Fu CS-MS was analyzed using FTIR and XRD. The XPS and EDS results confirmed the occurrence of a Schiff base reaction between glutaraldehyde (GA) and chitosan (CS), indicating successful encapsulation. TGA results revealed that the cross-linking reaction enhanced the thermal stability of the microspheres. Biocompatibility assessments using Human umbilical vein endothelial cells (HUVEC) line demonstrated the safety and compatibility of the fabricated microspheres. More importantly, the in vitro release results illustrated a pH-responsive drug release profile and sustained release of 5-Fu CS-MS for a period up to 60 days. In summary, the CS sustained-release microspheres produced using the microfluidic technique have the potential for intelligent response, precise drug delivery, and prolonged sustained release of small-molecule drugs. Download: Download high-res image (197KB) Download: Download full-size image
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DOI
高影响因子
危险品化学品经营许可证（带存储）