ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 11
·2893-2901
·
IF 4.157
Zhang Mengmei,Wang Hao,Bhandari Ramesh,Pan Qiuhui,Sun Fenyong
Selective separation and enrichment of phosphoproteins possess the distinct clinical and biological importance in the diagnosis, treatment, and management of several fatal human diseases. In this study, a facile synthesis of titanium(IV) ion–immobilized arsenate-modified poly(glycidyl methacrylate) microparticles (denoted as Ti 4+ -arsenate-PGMA-MPs) was developed for the efficient enrichment of intact phosphoproteins found in biologically complex protein samples. By virtue of the strong interaction between the titanium ions immobilized on the surface of Ti 4+ -arsenate-PGMA-MPs and phosphate groups of phosphoproteins, Ti 4+ -arsenate-PGMA-MPs had a high saturated adsorption capacity for phosphoproteins (901 mg/g for β-casein), which was much higher than that of non-phosphoproteins (73.5 mg/g for BSA). Ti 4+ -arsenate-PGMA-MPs were characterized by SEM, TEM, and FT-IR, and the average particle diameter was about 2.5 μm with good dispersibility. Besides, the application of Ti 4+ -arsenate-PGMA-MPs in real biological samples was investigated by SDS-PAGE analysis, and the results showed that Ti 4+ -arsenate-PGMA-MPs were able to enrich phosphoproteins efficiently. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 7
·9005-9017
·
IF 2.478
Mathivanan D.,Mohan A.,Yang Yaling
Nowadays, dopamine (DA) receives huge attention because it plays an importance role in chemical transmitter for peripheral and central nervous systems. 4-Nitrophenol (4-NP) is one of the common hazardous waste, because it can cause various serious diseases to humans and environment. Herein, a simple and highly efficient technique for the detection DA and 4-NP using nitrogen–ferric-doped carbon dots (N/Fe-CDs) was reported in this article. The N/Fe-CDs were prepared using microwave digestion technique with a high quantum yield of 49.52%. The N/Fe-CDs exhibited excellence selectivity toward the detection of DA and 4-NP. Based on the quenched fluorescence between N/Fe-CDs and DA and 4-NP to detect DA and 4-NP, the linear range of DA was 0.5 - 300 µM with a detection limit of 0.07 µM (S/N =3), while the linear range of 4-NP was 0.1 - 250 µM with a detection limit of 0.02μM. Additionally, the anticipated sensing technique has been successfully applied for real sample analysis such as human serum and urine samples for DA, lake water and tap water for 4-NP with satisfactory results. So, the proposed fluorescent probe is more precise detection for biological and environmental applications.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Coatings Technology and Research
·
2021
·Vol. 18
·期 4
·1027-1035
·
IF 2.382
Ding Wenfei,Zhao Zheng,Jiang Lingmei,Jian Xigao,Song Yuan,Wang Jinyan
Guidewires have crucial roles in intravascular, critical care, and urological applications. In recent years, the demand for guidewires with lower friction surfaces has arisen for medical applications. Among various surface lubrication strategies for guidewires, the most promising is the use of a hydrophilic coating. However, obtaining a hydrophilic coating on guidewires with strong adhesion remains difficult owing to the surface energy difference between the substrate and hydrophilic coating. Herein, we present a UV-curing semi-interpenetrating polymer network coating for medical thermoplastic polyurethane (TPU)-sheathed nitinol guidewires. A polyurethane acrylate (PUA) was synthesized and cured as a transition layer on the TPU substrate to provide strong adhesion. Then, polyvinyl pyrrolidone (PVP) and polyethylene glycol diacrylate (PEGDA) permeated into the PUA layer and cured to form the composite coating. The PUA–PEGDA–PVP coating exhibited excellent lubricity and satisfactory adhesion force. The surface coefficient of friction decreased to approximately 0.02 after modification with the coating, and the coating was stable during long-term soaking in water and repeated friction. In vitro toxicity tests revealed that the coating had good biocompatibility. Thus, our PUA–PEGDA–PVP coating has the potential for application as super-lubricity guidewires and other medical devices.
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-10
·
IF 14.919
Liu Gongquan,Zhao Yi-Lei,He Fangyuan,Zhang Peng,Ouyang Xingyu,Tang Hongzhi,Xu Ping
Biodegradation of aromatic and heterocyclic compounds requires an oxidative ring cleavage enzymatic step. Extensive biochemical research has yielded mechanistic insights about catabolism of aromatic substrates; yet much less is known about the reaction mechanisms underlying the cleavage of heterocyclic compounds such as pyridine-ring-containing ones like 2,5-hydroxy-pyridine (DHP). 2,5-Dihydroxypyridine dioxygenase (NicX) from Pseudomonas putida KT2440 uses a mononuclear nonheme Fe(II) to catalyze the oxidative pyridine ring cleavage reaction by transforming DHP into N -formylmaleamic acid (NFM). Herein, we report a crystal structure for the resting form of NicX, as well as a complex structure wherein DHP and NFM are trapped in different subunits. The resting state structure displays an octahedral coordination for Fe(II) with two histidine residues (His 265 and His 318 ), a serine residue (Ser 302 ), a carboxylate ligand (Asp 320 ), and two water molecules. DHP does not bind as a ligand to Fe(II), yet its interactions with Leu 104 and His 105 function to guide and stabilize the substrate to the appropriate position to initiate the reaction. Additionally, combined structural and computational analyses lend support to an apical dioxygen catalytic mechanism. Our study thus deepens understanding of non-heme Fe(II) dioxygenases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 6
·7574-7586
·
IF 2.478
Ni Xinyao,Feng Hao,Li Lihui,Liu Xiaoyun,Wang Tianyi,Cui Zhong-Kai,Gu Jinlou,Zhuang Qixin
Nanocomposites with great thermal stability and excellent dielectric properties play a vital role in the development of high-temperature resistant capacitors. A facile yet effective method to synthesize enhanced dielectric properties of thermostable three-phase poly(p-phenylene benzobisoxazole) (mCCTO@CFGO/PBO) nanocomposite films is demonstrated in this work. The amino-functionalized oxide CaCu 3 Ti 4 O 12 nanoparticles (mCCTO) are enwrapped by the carboxyl-functionalized ultrathin graphene shells (CFGO), forming the silk-cocoon structure to achieve homogeneous dispersion in the matrix. The final films are fabricated via in situ polymerization by introducing the mCCTO@CFGO nanoparticles into the PBO precursor polymer. The mCCTO@CFGO/PBO nanocomposite films exhibit high thermal stability (the 5% thermal decomposition temperature is 655 °C), while the unique silk-cocoon structure effectively confers the high dielectric constant up to 86.9 and ultra-low dielectric loss (0.063) with the loading of 10 wt% mCCTO@3 wt% CFGO/PBO at 1 kHz and 200 °C. This promising strategy accelerates the development of electronic devices used under high temperatures.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 2
·1-8
·
IF 2.253
Li Dehui,Xiao Jinglei,Gao Qiao,Hu Donghua,Yang Jing,Yuan Long
The synthesis of semiconductor nanocrystals (NCs) with low cost and in large scale is important for a variety of applications. In this work, a facile, non-vacuum solvothermal method was used to synthesize plate-shaped Cu 3 ZnInSnSe 6 (CZITSe) NCs with zincblende structure. Elemental mapping and XPS were utilized to establish the presence of all key elements. The bandgap of these CZITSe NCs is determined to be 1.22 eV. Thin films fabricated from the CZITSe NC suspension show clear photoresponse under illumination of a solar simulator, suggesting that these NCs can be photoabsorbers for thin-film solar cells. This study demonstrates a simple and economical method to synthesize CZITSe NCs with plate shape potentially useful for applications including solar energy conversion.
✓ 已验证
产品关联
DOI
高影响因子
INFLAMMATION
·
2021
·Vol. 44
·期 6
·2580-2591
·
IF 4.092
Hu Yubo,Ye Cong,Cheng Shuang,Chen Junyang
Propofol (PPF) is reported to play a protective role in ischemia/reperfusion (I/R) injury, including cerebral ischemia–reperfusion injury (CIRI). This study aims to investigate the mechanism by which PPF ameliorates CIRI. Kunming mice were used to establish the middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vivo. PPF pre-treatment was performed before CIRI. Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) levels were detected to evaluate the tissue injury. PC12 cells were exposed to hypoxia/reoxygenation (H/R) to construct the in vitro CIRI model, and PC12 cells were pre-treated with PPF before H/R. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression of lncRNA MALAT1 and miR-182-5p. Flow cytometry was used to detect the apoptosis of PC12 cells. Bioinformatics analysis, qRT-PCR, dual-luciferase reporter gene experiments, and RNA immunoprecipitation (RIP) experiments were performed to predict and validate the targeting relationship between MALAT1 and miR-182-5p. Western blot was used to detect Toll-like receptor 4 (TLR4) expression at protein level. PPF pre-treatment remarkably inhibited LDH and CPK levels in the serum of the mice with CIRI, and reduced the apoptosis of PC12 cells exposed to H/R. Besides, PPF pre-treatment markedly suppressed MALAT1 expression in both in vivo and in vitro models and upregulated miR-182-5p expression. MiR-182-5p was validated to be a downstream target gene of MALAT1, and MALAT1 could increase the expression of TLR4 by suppressing miR-182-5p. The effects of PPF on the injury of the mice brain and PC12 cells were partly counteracted by the restoration of MALAT1. PPF protects the brain against I/R-induced injury by regulating MALAT1/miR-182-5p/TLR4 axis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 15
·20295-20305
·
IF 2.478
Zhao Yaxin,Liu Dan,Yan Zhuqing,Zhang Zhidong,Zheng Yongqiu,Zhang Yanjun,Xue Chenyang
In recent years, the demand for wearable devices has promoted the research of flexible thermoelectric generators. Herein, this work reports a facile method to prepare the flexible Ag 2 Se/polyvinyl pyrrolidone (PVP) composite films. The Ag 2 Se nanorods are synthesized by the template method and then mixed with PVP solution to form the composite film optimized by the content of Ag 2 Se. The maximum power factor of the film is 16.18 µW m −1 K −2 at 320 K, and the thermoelectric generator (TEG) has an output voltage and power of 5.49 mV and 55.57 nW at a temperature difference of 40 K, respectively. Moreover, the Ag 2 Se/PVP film has good flexibility and can withstand multiple bending, the electrical conductivity reaching 89.8% and 76 % at 500 and 1000, respectively. PVP has an excellent protective capabilities effect so that the TE performance can remain stable in 40 days, and then the electrical conductivity only drops to 89 % of the initial value at 50 days. This work provides an effective method for preparing wearable thermoelectric devices, which can be extended to other composite materials.
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2021
·Vol. 15
·期 2
·1547-1553
·
IF 8.897
Gu Zhenkun,Wang Ying,Wang Shiheng,Zhang Ting,Zhao Rudai,Hu Xiaotian,Huang Zhandong,Su Meng,Xu Qun,Li Lihong,Zhang Yiqiang,Song Yanlin
Perovskite materials are promising candidates for the next generation of wearable optoelectronics. However, due to uncontrolled crystallization and the natural brittle property of crystals, it remains a great challenge to fabricate large-scale compact and tough perovskite film. Here we report a facile method to print large-scale perovskite films with high quality for flexible photodetectors. By introducing a soluble polyethylene oxide (PEO) layer during the inkjet printing process, the nucleation and crystal growth of perovskite is well controlled. Perovskite films can be easily printed in large scale and patterned in high resolution. Moreover, this method can be extended to various kinds of perovskite materials, such as MAPbI 3 (MA = methylammonium), MA 3 Sb 2 I 9 , and (BA) 2 PbBr 4 (BA = benzylammonium). The printed perovskite films show high quality and excellent mechanical performance. The photodetectors based on the MAPbBr 3 perovskite films show a responsivity up to ∼ 1,036 mA/W and maintain over 96.8% of the initial photocurrent after 15,000 consecutive bending cycles. This strategy provides a facile approach to prepare large-scale flexible perovskite films. It opens up new opportunities for the fabrication of diverse wearable optoelectronic devices.
✓ 已验证
产品关联
DOI
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 4
·992-1000
·
IF 6.15
Wu Xiao-yan,Lv Jin-yan,Zhang Shi-qing,Yi Xin,Xu Zi-wei,Zhi Yuan-xing,Zhao Bo-xin,Pang Jian-xin,Yung Ken Kin Lam,Liu Shu-wen,Zhou Ping-zheng
Dysregulation of NLRP3 inflammasome results in uncontrolled inflammation, which participates in various chronic diseases. TWIK2 potassium channel mediates potassium efflux that has been reported to be an essential upstream mechanism for ATP-induced NLRP3 inflammasome activation. Thus, TWIK2 potassium channel could be a potential drug target for NLRP3-related inflammatory diseases. In the present study we investigated the effects of known K2P channel modulators on TWIK2 channel expressed in a heterologous system. In order to increase plasma membrane expression and thus TWIK2 currents, a mutant channel with three mutations (TWIK2 I289A/L290A/Y308A ) in the C-terminus was expressed in COS-7 cells. TWIK2 currents were assessed using whole-cell voltage-clamp recording. Among 6 known K2P channel modulators tested (DCPIB, quinine, fluoxetine, ML365, ML335, and TKDC), ML365 was the most potent TWIK2 channel blocker with an IC 50 value of 4.07 ± 1.5 μM. Furthermore, ML365 selectively inhibited TWIK2 without affecting TWIK1 or THIK1 channels. We showed that ML365 (1, 5 μM) concentration-dependently inhibited ATP-induced NLRP3 inflammasome activation in LPS-primed murine BMDMs, whereas it did not affect nigericin-induced NLRP3, or non-canonical, AIM2 and NLRC4 inflammasomes activation. Knockdown of TWIK2 significantly impaired the inhibitory effect of ML365 on ATP-induced NLRP3 inflammasome activation. Moreover, we demonstrated that pre-administration of ML365 (1, 10, 25 mg/kg, ip) dose-dependently ameliorated LPS-induced endotoxic shock in mice. In a preliminary pharmacokinetic study conducted in rats, ML365 showed good absolute oral bioavailability with F value of 22.49%. In conclusion, ML365 provides a structural reference for future design of selective TWIK2 channel inhibitors in treating related inflammatory diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2021
·Vol. 15
·期 2
·1027-1038
·
IF 8.897
Ouyang Zhaofeng,Cui Songbo,Yu Houyong,Xu Dewen,Wang Chuang,Tang Dongping,Tam Kam Chiu
Flexible wearable electronics were developed for applications such as electronic skins, human-machine interactions, healthcare monitoring, and anti-infection therapy. But conventional materials showed impermeability, single sensing ability, and no designated therapy, which hindered their applications. Thus it was still a great challenge to develop electronic devices with multifunctional sensing properties and self-driven anti-infection therapy. Herein, flexible and breathable on-skin electronic devices for multifunctional fabric based sensing and self-driven designated anti-infection therapy were prepared successfully with cellulose nanocrystals/iron(III) ion/polyvinyl alcohol (CNC/Fe 3+ /PVA) composite. The resultant composite films possessed robust mechanical performances, outstanding conductivity, and distinguished breathability (3.03 kg/(m 2 ·d)), which benefited from the multiple interactions of weak hydrogen bonds and Fe 3+ chelation and synergistic effects among CNC, polyaniline (PANI), and PVA. Surprisingly, the film could be assembled as a multifunctional sensor to actively monitor real-time physical and infection related signals such as temperature, moisture, pH, NH 3 , and human movements even at sweat states. More importantly, this multifunctional device could act as a self-driven therapist to eliminate bacterial by the release of Fe 3+ , which was driven by the damage of metal coordination Fe-O bonds due to the high temperature caused by infection at wound sites. Thus, the composite films had potential versatile applications in electronic skins, smart wound dressings, human-machine interactions, and self-driven anti-infection therapy.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 7
·1-13
·
IF 5.833
Wang Xiao-han,Li Wei,Jiang Hong-xin,Chen Yan,Gao Rong-zhi,Tang An-na,Kong De-ming
A heteropore covalent organic framework (COF)-based composite membrane material was prepared and proved to have a satisfactory effect on the pretreatment of vegetable samples. The composite membrane was fabricated by in situ growth of a dual-pore COF on the surface of polydopamine (PDA)-aminated non-woven (NW) fabric. Due to the difference in the strength of the interaction between the phytochromes/COF and the pesticides/COF, the removal of phytochromes and the recovery of pesticides can be achieved by adjusting the composition of the solution. Through a simple immersion or filtration operation, NW@PDA@COF composite membrane can quickly and almost completely remove interfering phytochromes in the samples. The recovery of pesticides was determined by HPLC-MS/MS, and the recovery efficiencies were 72.3~101.7% and 67.3~106.7% for immersion and filtration modes of five different vegetable samples, respectively; the RSD is between 1.1 and 19% ( n = 3). The limits of detection and quantification for the 13 pesticides investigated were 0.08 μg·L −1 and 0.23 μg·L −1 , respectively. A wide linear range of 1~1000 μg·L −1 was observed with R 2 values from 0.9774 to 0.9998. The membrane can be repeatedly used for at least 10 times by using a facile elution treatment. Compared to other commonly used sample pretreatment materials, heteropore COF-based composite membrane is superior in terms of sorbent amount, treatment time, operation simplicity, and material reusability. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）