RSC Advances
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2025
·Vol. 15
·期 27
·21300-21310
·
IF 4.6
Yu-Ling Qi,Hao-Yu Zhou,Guo-Zhi Han
Self-healing hydrogels have the ability to repair themselves at the incision after being damaged and can return to their original state of morphology and performance. However, constructing a hydrogel with superior mechanical strength and tensile properties after self-healing remains a challenge. In this work, using polyvinyl alcohol, borax, chitosan, and a type of SiO2@g-C3N4@TiO2 nanoparticles as raw materials, a novel photo-regulated self-healing hydrogel was developed using a freezing–thawing method, which could achieve a synchronous increase in Young's modulus and tensile strength under visible light irradiation during the self-healing process. In addition, the doping of the SiO2@g-C3N4@TiO2 nanoparticles improved the self-healing performance of the PVA-based hydrogels. With the addition of a trace amount of SiO2@g-C3N4@TiO2 nanoparticles, the self-healing efficiency of the hydrogel increased from 26.67% to 45.67% in darkness and from 41.33% to 65.67% under visible light irradiation.
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高影响因子
BIOSENSORS & BIOELECTRONICS
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2012
·
IF 5.602
Qiaoli Yue,Tongfei Shen,Changna Wang,Lei Wang,Haibo Li,Shuling Xu,Huaisheng Wang,Jifeng Liu
Conjugations of oligonucleotides , chromophores , and gold nanoparticles (GNPs) can be used for energy transfer assays to detect DNA . Herein, a homogenous Förster resonance energy transfer (FRET) system employing two-step modification of oligonucleotide on GNPs was reported. The distance between the donor (fluorescein attached onto DNA) and the acceptor (GNPs) was controlled by using the G-rich DNA. In the presence of porphyrin or berberine , which can act as ligands of G-quadruplexes, the G-rich DNA spacer can result into G-quadruplex structure. Therefore, the intimate contact between the fluorophore and the GNP results in efficient energy transfer and fluorescence quenching . After hybridization with target DNA, the G-quadruplex stretched and resulted in an enhancement of fluorescence. So the present FRET system can be used for target DNA sensing with detection limit as low as 40 pM ( S / N =3). In this study, a relation between the fluorescence quenching efficiency and GNP sizes was found and bigger GNPs had higher fluorescence enhancement after hybridization with target DNA.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Fei Li,Huanshun Yin,Yan Chen,Siyu Wang,Jingyan Li,Yuemei Zhang,Chunxiao Li,Shiyun Ai
DNA formylation (5-formylcytosine, 5fC) is a major epigenetic modification involved in alterations in the DNA double helix structure and protein identification. Due to the low amount in all mammalian tissues and cells, it is necessary to develop a rapid, sensitive and efficient method for detecting 5fC for further understanding the biological functions of 5fC. Thus, a novel PEC biosensor was constructed using P-g-C 3 N 4 -WS 2 nanocomposite as photoactive material. Firstly, AuNPs/P-g-C 3 N 4 -WS 2 /ITO electrode was prepared as substrate electrode. Secondly, the probe DNA and complementary DNA (containing 5fC base) was modified to the electrode surface based on the formation of Au-S bonds between AuNPs and thiol group on the probe DNA and hybridization, respectively. Finally, the amino functionalized MnO 2 nanoflowers were further modified to the electrode surface by covalent interaction between the aldehyde group on the 5fC and the amino group on MnO 2 nanoflowers. The sensitive and specific detection of 5fC can be achieved by oxidizing ascorbic acid with MnO 2 nanoflowers and quenching the photoactivity of P-g-C 3 N 4 -WS 2 nanocomposite. The sensor has a detection range of 0.01–200 nM and a detection limit of 3.8 pM. Moreover, this sensor has excellent detection specificity, stability and reproducibility.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Jiujun Wang,Ya Wu,Qinghua Wu,Li Li,Yun Wang,Hualin Yang
Melamine has a high nitrogen content thus is often illegally added to milk powder to phonily boost the protein percentage composition. Milk products containing melamine are harmful to babies and children. In the present work, a simple, label-free and turn-on fluorescence method to detect melamine with a high sensitivity and selectivity has been developed. The N -methylmesoporphyrin IX (NMM)/G-quadruplex structure is used as a signal reporter in this method to realize label-free detection of melamine. The detection limit of this method is 12.5 nM, which is much less than the maximum level permitted for infant formulas. The linear range is 0.05–1 μM ( R 2 = 0.98). The selectivity of this method is also very satisfactory. Furthermore, this method can be successfully applied for detection melamine in raw milk and milk powder.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Li Li,Yuanqian Xiong,Yuqian Guo,Hong Duan,Yuankui Leng,Xiaolin Huang,Gang Chen,Yonghua Xiong
DNA-templated silver nanoclusters (tDNA-AgNCs) show considerable promise as fluorescence reporters for the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system because of their ease of synthesis, strong resistance to photobleaching, and large Stokes shift. However, the weak luminous intensity and low cleavage efficiency of tDNA-AgNCs limit the sensitivity of CRISPR assays. In this study, we developed a novel approach by introducing activator DNA (aDNA) with a G-quadruplex structure to enhance the luminous intensity of the tDNA-AgNCs. As a result of the high cleavage efficiency of free aDNA by activated CRISPR/Cas12a, the G-quadruplex-enhanced tDNA-AgNCs (GED-AgNCs) were integrated into recombinase polymerase amplification and CRISPR/Cas12a system for the ultrasensitive detection of Salmonella typhimurium. By optimizing the synthesis of tDNA-AgNCs and GED-AgNCs, our developed G-quadruplex-enhanced DNA-AgNC CRISPR assay (G-DACA) platform enabled the sensitive determination of S. typhimurium with a detection limit as low as 1 CFU/mL and a wide dynamic range of 10–108 CFU/mL. Moreover, our proposed method demonstrated good accuracy and reliability for the quantitative analysis of S. typhimurium in real pasteurized milk samples, with recovery rates ranging from 81.06% to 102.33% and relative standard deviations between 7.17% and 14.84%. Overall, our innovative G-DACA platform offers an economical and versatile solution for food safety and clinical diagnostics.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Hongliang Tan,Gonge Tang,Chanjiao Ma,Qian Li
In this work, we developed a simple and sensitive method for the detection of cysteine (Cys) by employing terbium ion (Tb 3+ )-promoted G-qudraplex (G4/Tb) as a luminescent probe, which is based on Ag + -mediated conformational change of G4/Tb. Due to Ag + is able to compete with Tb 3+ to bind guanine at G4, the presence of Ag + can lead to the formation of G4/Tb–Ag + complex and disrupt the structure of G4/Tb. Meanwhile, the binding of Ag + with G4/Tb will also cause the alteration of the excited state of G4 and more efficient energy transfer from G4 to Tb 3+ , enhancing the luminescence of G4/Tb. However, upon the addition of Cys, Ag + will be released from G4/Tb–Ag + complex because of the high affinity of Cys to Ag + . This results in the re-formation of the conformation of G4/Tb and the decrease of the luminescence of G4/Tb. So, Ag + -enhanced luminescence of G4/Tb is associated with its conformational transformation. As a luminescent probe for Cys, G4/Tb not only shows excellent selectivity and sensitivity with a detection limit of 20 nM, but also possesses the features of simple preparation, easy reproducibility, and eliminating the interferences from background fluorescence. We envision that the presented strategy might provide new insight into the biosensing applications of lanthanide complex.
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Molecular Medicine Reports
·
2017
·Vol. 16
·期 5
·5997-6003
·
IF 1.692
Xiang Feng,Liyuan Zhou,Xun Mao,Chao Tong,Xuyang Chen,Diqi Zhao,Philip N. Baker,Yinyin Xia,Hua Zhang
Preeclampsia is a pregnancy‑specific disorder, which is a leading cause of maternal and perinatal mortality and morbidity. A lower increase of estrogen, compared with the increase in progesterone, is associated with pathogenesis of the disease during pregnancy. G‑protein‑coupled receptor 30 (GPR30) mediates the action of estrogen, however remains to be investigated in preeclampsia. The levels of GPR30 were measured in placentae from uncomplicated pregnancies and pregnancies complicated by preeclampsia using immunohistochemistry and western blotting. GPR30 expression was additionally measured in placental HTR8/SVneo cells following 17β‑estrogen (E2) treatment in normal or hypoxia‑reoxygenation conditions by western blotting. In addition, the outgrowth of HTR8/SVneo cells following E2 treatment in hypoxia‑reoxygenation conditions was measured. Levels of GPR30 were significantly reduced in placentae from women with preeclampsia as compared with uncomplicated pregnancies. Treatment with E2 significantly increased the expression of GPR30 in HTR8/SVneo cells, in normal and hypoxia‑reoxygenation conditions. Furthermore, treatment with E2 increased the outgrowth of HTR8/SVneo cells in hypoxia‑reoxygenation conditions. The present study demonstrated lowered placental expression of GPR30 in preeclampsia. Estrogen treatment increases GPR30 expression in extravillous trophoblast and GPR30 may be involved in extravillous trophoblast invasion.
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iScience
·
2023
·
IF 5.8
Huo Xu,Fenglin Yang,Danlong Chen,Weilin Ye,Guohui Xue,Lee Jia
MicroRNAs (miRNAs) play an important biomarker in various biological processes, especially cancer related, yet economic, simple, sensitive and specific methods for miRNA determination are still challenging. In this study, we have developed stepwise-strand displacement amplification (S-SDA)-based colorimetric sensing platform for let-7a miRNA detection in clinical serum samples. Our results demonstrated that the developed S-SDA-based method shows high sensitivity with a detection limit of 63.2 pM and a naked eye detection limit of 0.1 nM. Moreover, the S-SDA amplifier is able to discriminate target miRNAs from their mutants with high accuracy and specificity. With its high sensitivity and selectivity, this method successfully identified healthy individuals from patients with colon cancer by detecting let-7a miRNAs in serum. We believe the colorimetric analysis method will provide a new paradigm for the detection of miRNA with different abundance and show great potential for clinical application in biomedical analysis and early clinical diagnosis.
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高影响因子
PHYTOTHERAPY RESEARCH
·
2020
·Vol. 34
·期 6
·1409-1420
·
IF 4.087
Chuchu Qiao,Shiling Hu,Delu Che,Jue Wang,Jiapan Gao,Ruiping Ma,Wenjun Jiang,Tao Zhang,Rui Liu
Mast cells play an important role in inflammatory and allergic diseases. MAS-related G protein-coupled receptor X2 (MRGPRX2) is a novel G protein-coupled receptor in mast cells that mediates drug-induced anaphylactoid reactions. Piperine has been reported to have anti-inflammatory and anti-allergic pharmacological activities. However, whether the pharmacological effects are regulated by MRGPRX2 has not yet been reported. The purpose of this study was to assess the anti-anaphylactoid effect of Piperine and to explore its potential mechanism. The anti-anaphylactoid effect of Piperine was assessed by an in vivo mouse hindpaw extravasation model. Mast cell intracellular calcium mobilization was measured by a calcium imaging assay. An enzyme immunoassay was used to evaluate the release of pro-inflammatory factors from stimulated mast cells. Activated mast cell related signals were assessed by western blot. A cell membrane chromatography assay was used to determine the binding characteristics of Piperine and MRGPRX2. The results showed that Piperine suppressed mast cell intracellular Ca 2+ mobilization, inhibited cytokines and chemokines release, and down-regulated the phosphorylation level of phospholipase Cγ1, protein kinase C, inositol 1,4,5-triphate receptor, P38, protein kinase B, and ERK. Meanwhile, Piperine can bind to MRGPRX2 as a specific antagonist. Hence, Piperine can be served as a novel therapeutic drug candidate for MRGPRX2-mediated anaphylactoid reactions.
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高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Yu-Zhuang Gao,Tian-Xiao Chang,Yi-Xian Wu
Biomass sodium alginate (SA) and biomass-derived ether tetrahydrofuran (THF) have drawn considerable attention due to their sustainability and bio-renewability. In this paper, the novel acylated sodium alginate- g -polytetrahydrofuran graft copolymers (ASA- g -PTHF) with various PTHF branches ( M n,PTHF = 600–4800 g/mol, G N = 3–20 per 1000 SA monosaccharide) could be successfully synthesized by combination of THF living cationic ring-opening polymerization with grafting-onto method. The microphase separation of ASA- g -PTHF graft copolymer formed via self-assembly from two kinds of segments in backbone and side chains and was emphasized by thermal annealing, leading to a remarkable increase in water contact angle on polymer surface even by 38.4°. The hydrophilicity and crystallization behavior of ASA- g -PTHF can be decreased with increasing M n,PTHF and G N , which confine the crystallization of graft copolymer. ASA- g -PTHF microspheres (~1 μm) loaded with ibuprofen shows pH sensitivity and reach 100% of drug-release at pH = 7.4 within 30 h. ASA- g -PTHF exhibits resistance to bovine serum albumin adsorption and its composite with small-scaled silver nanoparticles (Ag-NPs, <2.6 wt%, 5–15 nm) generated in - situ behaves good antibacterial performance against E. coli and S. aureus . The ASA- g -PTHF would contribute to the better utilization of biomass and has promising for biomedical applications.
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CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Wen li Zhao,Yan hua Gao,Dongfeng Zhang
In this paper, spherical g-C 3 N 4 modified by N defect and O doping was prepared by a simple hydrothermal method , and combined with CdS to form a direct Z-type heterojunction . Combined with a series of characterizations (XRD, SEM, BET, FTIR and UV–vis DRS), the results show that the addition of CTAB brings a lot of positive charges on the surface of N-OCN. According to the principle of electrostatic attraction, positively charged N-OCN negatively charged CdS attract each other, which strengthens the close contact between CdS and N-OCN and plays an important role in stabilizing the formation of heterojunction. CdS is anchored to N-OCN through electrostatic interaction. Under the irradiation of visible light, the electrons on CB on positively charged N-OCN combine with holes on negatively charged CdS valence band, which improves the redox ability in photocatalytic reaction system. Taking methyl orange (MO) as a pollutant degradation model, the possible degradation mechanism was discussed in detail. This study provides a simple, effective and wide application method for the modification of g-C 3 N 4 .
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DIAMOND AND RELATED MATERIALS
·
2021
·
IF 3.315
Yanhua Gao,Wenli Zhao,Dongfeng Zhang
In this paper, the growth mechanism of prismatic carbon nitride at different temperatures was studied by using the mixed solution of CTAB and NaOH by thermal polycondensation-hydrothermal method, and a series of characterization methods were used to analyze and verify. The pollutant model MO solution was degraded and its photocatalytic activity was investigated under light. The results show that at a relatively low temperature, g-C 3 N 4 is a rod-like structure, and NaOH plays a major role. With the increase of temperature, CTAB plays a major role and can transport the primitives to form a rod, but also forms an ordered hydrophobic film on the crystal plane, which inhibits the growth of the rod-like structure on the (100) crystal plane and controls the extension on the (002) crystal plane. Its degradation rate is about 2.64 times that of pure g-C 3 N 4 . This significantly improves the separation rate of photogenerated electrons and the photocatalytic degradation efficiency.
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