Scientific Reports
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2022
·Vol. 12
·期 1
·1-12
·
IF 4.996
Zhang Zhi-xuan,Lv Lu,Shi Ai-hua,Li Yun-hao,Wang Tian-ren,Guo Yuan-hang,Hu Bao-juan,Yan Xiao-peng,Fu Jun-ke,Ma Feng,Wang Hao-hua,Lv Yi,Zhang Yong
Many methods are used to locate preoperative small pulmonary nodules. However, deficiencies of complications and success rates exist. We introduce a novel magnetic gel for small pulmonary nodules localization in rabbit model, and furtherly evaluate its safety and feasibility. Rabbits were used as the experimental objects. A magnetic gel was used as a tracer magnet, mixed as sodium alginate-Fe 3 O 4 magnetic fluid and calcium gluconate solution. In short-term localization, a coaxial double-cavity puncture needle was applied to inject the gel into the lung after thoracotomy, and a pursuit magnet made of Nd-Fe-B permanent magnetic materials was used to attract the gel representing location of the nodule. In long-term localization, the gel was injected under X-ray guidance. Imaging changes to the lung were observed under X-ray daily. Thoracotomy was performed to excise tissue containing the gel, and hematoxylin–eosin staining was used to observe the tissue on postoperative days 1, 3, 5, and 7. Observe tissues morphology of heart, liver, spleen, and kidney in the same way. The gel was formed after injection and drew lung tissue to form a protrusion from the lung surface under the applied magnetic field. No complication was observed. The shape and position of the gel had not changed when viewed under X-ray. Pathological analysis showed the gel had a clear boundary without diffusion of magnetic fluid. All tissues retained good histologic morphology and no magnetic fluid was observed. Our study preliminarily suggested that the technique using sodium alginate-Fe 3 O 4 magnetic gel to locate small pulmonary nodules with guidance of X-ray, and to search for them under an applied magnetic field during the operation is safe and feasible.
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高影响因子
DIGESTIVE DISEASES AND SCIENCES
·
2022
·Vol. 68
·期 3
·778-790
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IF 3.487
Gu Dan,Cao Ting,Yi Shijie,Liu Ya,Fan Chao
Background Ulcerative colitis (UC) features chronic, non-infectious inflammation of the colon. Insulin-like growth factor binding protein 5 (IGFBP5) has been indicated to be related to various inflammation-related diseases. However, its association with UC remains largely unclear. Aims Here, we investigate the role of IGFBP5 in colonic mucosal epithelial cell injury in UC. Methods Differentially expressed genes in the colonic tissues of UC mice were screened using the Gene Expression Omnibus database, and IGFBP5 was identified. UC mice were developed using dextran sulfate sodium, and IGFBP5 expression in the colonic tissues of UC mice was confirmed by immunohistochemistry and RT-qPCR. The effects of IGFBP 5 in vivo and in vitro were investigated by intraperitoneal injection of adeno-associated virus into UC mice or by transfection with an IGFBP5 overexpression plasmid into lipopolysaccharide-treated colonic mucosal epithelial cells. The mechanisms causing IGFBP5 deletion in UC were predicted by bioinformatics analysis and ChIP-qPCR and verified by rescue experiments. Results IGFBP5 was reduced in UC. IGFBP5 impaired the NFκB pathway in the colonic tissue of UC mice and ameliorated inflammatory infiltration and colonic mucosal cell injury. IGFBP5 depletion was associated with H3K27me3 modification, which was induced by EZH2. CTCF was responsible for recruiting EZH2 to the promoter region of IGFBP5. CTCF inhibition repressed UC progression by reducing H3K27me3 modification via the discouragement of the enrichment of EZH2 in the IGFBP5 promoter. Conclusions CTCF modulates H3K27me3 modification of the IGFBP5 promoter by recruiting EZH2, thereby downregulating IGFBP5 to accentuate colonic mucosal epithelial cell injury in UC mice.
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POLYMER BULLETIN
·
2022
·Vol. 80
·期 5
·4949-4964
·
IF 2.843
Alassod Abeer,Khalaji Mina Shahriari,Islam Syed Rashedul,Xu Guangbiao,Chaudary Aneeba,Alam Md. Kowsar,Alkhateeb Weaam
Creating inexpensive, highly efficient, and recyclable sponges for water remediation is significant but remains a big challenge. Herein, this research reported a facile method to fabricate cost-effective, environmentally friendly, and reusable sponges by blending Polypropylene with renewable resources (chitosan) with different ratios via thermal induce phase separation. The properties of obtained sponge were deeply evaluated in terms of the thermogravimetric analyzer, Fourier transforms infrared measurement, and scanning electron microscopy. Besides, wettability properties were analyzed. Newly sponges showed better thermal properties than Polypropylene with increased chitosan of more than 20 wt%. Chemical characterization confirmed successfully blending between components. Additionally, the wettability properties were influenced by increasing chitosan loading, where the water contact angle increased more than 130° due to increased surface roughness. These results revealed that new sponges are an ideal alternative sorbent for oil spill cleanup. The sorption ability increased with an increase of chitosan loading, where sponge PP40CH registered the highest sorption ability, more than 700%, with oil retention ability of more than 80%. The obtained sponge could be used as a promising filter screen for separating oils and organic liquid from contaminated water with more than 90% separation efficiency. Thus, thermal induce phase separation is an easy, eco-friendly, cost-effective, and practical way to produce a blend sponges with great potential usage prospects in purification and oil spill cleanup and make new sponges more competitive promising candidates than commercial absorbents.
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APOPTOSIS
·
2022
·Vol. 27
·期 7
·577-589
·
IF 5.561
Ma Liang,Xuan Xiaojing,Fan Minghui,Zhang Yumeng,Yuan Guozan,Huang Guozheng,Liu Zi
Chemotherapy represents one of the main conventional therapies for breast cancer. However, tumor cells develop mechanisms to evade chemotherapeutic-induced apoptosis. Thus, it is of great significance to induce non-apoptotic cell death modes, such as paraptosis, in breast cancer. Herein, a novel 8-hydroxyquinoline derivative, 5,7-dibromo-8-(methoxymethoxy)-2-methylquinoline (HQ-11), was obtained and its potential anti-breast cancer mechanisms were investigated. Our results showed that extensive cytoplasmic vacuoles derived from the endoplasmic reticulum (ER) and mitochondria were appeared in MCF7 and MDA-MB-231 breast cancer cells by HQ-11 incubation, and pretreatment of cycloheximide was able to inhibit this vacuolation and HQ-11-induced cell death, showing the characteristics of paraptosis. ER stress was involved in HQ-11-caused paraptosis evidenced by the increase of glucose-regulated protein 78, C/EBP homologous protein and polyubiquitinated proteins. Molecular docking analysis revealed a favorable binding mode of HQ-11 in the active site of the chymotrypsin-like β5 subunit of the proteasome, indicative of proteasome dysfunction under HQ-11 treatment, which might result in further aggravated ER stress. Furthermore, treatment of HQ-11 resulted in increased phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun NH 2 -terminal kinase, and inhibition of ERK with U0126 significantly attenuated HQ-11-induced ER stress and paraptosis. In addition, exposure to HQ-11 also caused apoptosis in breast cancer cells partially through activation of ERK pathway. All these results conclusively indicate that HQ-11 triggers two distinct cell death modes via inhibition of proteasome and activation of ERK pathway in breast cancer cells, providing a promising candidate in future anti-breast cancer therapy.
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JOURNAL OF MATERIALS SCIENCE
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2022
·Vol. 57
·期 25
·11783-11795
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IF 4.682
Li Yuwen,Ding Fengyun,Zhou Linsen,Xiao Kela,Lv Chao,Li Peilong,Zhang Zexuan,Hu Cun,Shi Yan,Song Jiangfeng,Zhang Huaiqiang,Zhang Xin
Methane decomposition is an efficient pathway to obtain high-purity hydrogen and valuable carbon fibers, nanotubes, etc. In this work, a series of NiCo alloy catalysts with different Ni/Co ratios are successfully prepared using NiCoAl ternary layered double hydroxide (LDH) as precursors. By comparison with dual-LDH-derived catalysts and alumina-based catalysts, we found that the reduced catalysts from LDH have the characteristics of uniform particle size and distribution, and in subsequent the methane decomposition reaction over NiCo alloy catalysts show high catalytic activity and selectivity for carbon nanotubes. Furthermore, density functional theory (DFT) method is carried out to elucidate the catalytic decomposition mechanism of methane into hydrogen and carbon over a series of Ni n Co 4− n ( n = 0–4). These combined experimental and theoretical studies show that the introduction of Co element benefits the process of C–H dissociation. These combined experimental studies and DFT calculations can provide insights into the development of methane decomposition and other Ni-catalyzed methane reactions. Graphical abstract
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Journal of Pharmaceutical Investigation
·
2022
·Vol. 52
·期 5
·639-648
·
IF 0
Lei Fang,Zhang Huimin,Luo Rui,Fei Qingsong,Bai Luyu,He Ning
Purpose The present study was designed to formulate a thermoreversible in situ gel system for sustained ocular delivery of desmopressin acetate (DDAVP) for the treatment of polyuria. Methods The cold method was employed for the preparation of in situ gel formulations using different levels of poloxamer 407 (F-127) and carboxymethyl chitosan (CMCTS). The optimal formulation was selected based on gelation temperature, rheological behavior, and in vitro drug release, and further evaluated by thermal, ocular irritation, residence time, in vivo pharmacokinetics, and pharmacodynamic analyses. Results The optimal formulation, composed of F-127 (20%) and CMCTS (1.5%), showed appropriate fluidity at room temperature (25 °C) and transitioned to semisolid gels at physiological temperature (34 °C). The optimal gels showed pseudoplastic fluid properties and favorable thixotropy characteristics in rheological tests. Differential scanning calorimetry analysis revealed even dispersal of DDAVP in the matrix of gels. Results from in vivo studies demonstrated that the newly prepared gel successfully prolonged the residence time of DDAVP, improved ocular bioavailability, and exerted the same antidiuretic effect as ophthalmic drops. Conclusion The developed thermoreversible ophthalmic in situ gel of DDAVP presents a promising formulation for the treatment of polyuria.
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Scientific Reports
·
2022
·Vol. 12
·期 1
·1-11
·
IF 4.996
Pan Yixiao,Wang Jiahao,Jiang Zichao,Guo Qi,Zhang Zhen,Li Jingyi,Hu Yihe,Wang Long
Medicine treatments for bone-related diseases such as osteoporosis, bone metastasis, osteomyelitis, and osteolysis are often limited by insufficient drug concentration at the lesion sites owing to the low perfusion of bone tissue. A carrier that can deliver multiple bone destruction site-targeting drugs is required to address this limitation. Here, we reported a novel bone-targeting nano-drug delivery platform formed by the integration of zoledronate (ZOL) and zeolitic imidazolate framework-8 (ZIF-8) nanoparticles. The ZOL mixed zeolitic imidazolate framework (ZZF) nanoparticles were synthesized in water at room temperature (25 °C), where many biomacromolecules could maintain their activity. This allowed the ZZF nanoparticles to adapt the encapsulation ability and pH response release property from ZIF-8 and the excellent bone targeting performance of ZOL simultaneously. Considering the ease of preparation and biomacromolecule-friendly drug delivery of this nano platform, it may be useful in treating bone-related diseases.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2022
·Vol. 331
·期 9
·3905-3913
·
IF 1.754
Guo Zong,Chu Taiwei
In this work, a separation and purification process of U(VI), Zr(IV) and I 2 from various fission products in nitric acid solution by 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([C 4 mim][NTf 2 ]) and dibutyl-3-(3-methylimidazolium)propylphosphoramide bis(trifluoromethanesulfonyl)imide-task specific ionic liquid (phosphoramide-TSIL) was investigated. The phosphoramide-TSIL was found to reduce ligand loss compared to dibutyl propylphosphoramidate by total organic carbon testing (TOC). The stripping of U(VI), Zr(IV) and I 2 could be achieved by guanidine carbonate solution, oxalic acid solution and sodium hydroxide solution, respectively. The phosphoramide-TSIL/[C 4 mim][NTf 2 ] showed good irradiation stability and recyclability. Phosphoramide-TSIL has the potential to be applied for separation and purification in the actual radioactive environment. Graphical abstract
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ACCREDITATION AND QUALITY ASSURANCE
·
2022
·Vol. 27
·期 4
·243-248
·
IF 0.856
Chen Chao,Wang Haifeng,Li Jia,Sun Guohua
The mass fraction of inorganic impurities of a pure organic substance can be determined by the residue on ignition method and then used to calculate the purity according to mass balance theory. The conventional residue on ignition method is insensitive. In this study, a novel crucible made of annealed aluminum foil was used instead of the conventional crucible. The mass of the annealed aluminum crucible on ignition was repeatable (standard deviation of 1.4 µg). The mass of the annealed aluminum crucible remained stable in the ambient atmosphere because no water adsorbed to the surface of annealed aluminum. When annealed at high temperature, the mass of the aluminum crucible increased owing to the oxidation of aluminum. On subsequent annealing, the amorphous aluminum oxide (Al 2 O 3 ) film transformed into a γ-Al 2 O 3 film which prevented the inner aluminum from oxidation and adsorption of water. The limit of quantification of the mass of inorganic impurities determined by the residue on ignition method using the annealed aluminum crucible (30 µg) was much lower than that using a platinum crucible (500 µg). When the mass of solute of sodium chloride (NaCl) aqueous solution was in the range 20 µg–200 µg, the recovery of the NaCl mass fraction was in the range 96 %–104 %. The residue on ignition method was applied to eight pure organic substances. The improved residue on ignition method using the annealed aluminum crucible determined the mass fractions of inorganic impurities of the pure organic substances with high sensitivity and accuracy.
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CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 8
·743-754
·
IF 2.213
Lin Min,Li Xinxin,Zhang Hongfeng,Ji Wenquan,Shi Yuhan,Li Qinran,Zhang Chenxi,Deng Qiliang
Abstract The C-terminal epitope imprinted polymers on the silica were prepared by reversible addition-fragmentation chain transfer (RAFT) strategy with C-terminal nonapeptide of cytochrome c (Cyt c) as the template. 4-cyano-4-(phenylcarbonothioylthio) phetanoic acid was immobilized on the silica as a chain transfer agent to regulate the polymerization of the imprinted layer to enhance the recognition performance of the imprinted materials for the peptide and target protein. Additionally, zinc methacrylate was used as a functional monomer to form the imprinted sites through the synergistic effect of Zn 2+ chelating, hydrogen bonding and electrostatic attraction, which further improved the recognition between polymers and protein. The molecularly imprinted polymers prepared with RAFT strategy show good recognition for the epitope peptide and Cyt c with imprinting factors of 7.96 and 6.17, respectively, which is higher than those prepared without RAFT strategy. Furthermore, the imprinted polymers have a good binding capacity and imprinting factor for Cyt c in multi-proteins selective recognition and have good reusability. The performance of Cyt c recognition in bovine serum by the epitope imprinted polymers was calculated by HPLC and the results demonstrated the well selectivity and potential application for Cyt c recognition in biological sample. Graphical abstract
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-8
·
IF 6.408
Li Shuhuai,Luo Jinmei,Wu Yuwei,Ma Xionghui,Pang Chaohai,Wang Mingyue,Luo Jinhui,Zhang Chenghui,Tan Gaohao
Considering the limitations associated with existing methods for the detection of trace amounts of trichlorfon, this paper proposes a novel molecularly imprinted electrochemiluminescence (ECL) sensor for the detection of trichlorfon by utilizing the double enhancement effect of trichlorfon and Ag nanoparticles supported by multi-walled carbon nanotubes (MWCNTs/Ag NPs) in a luminol–H 2 O 2 ECL system. Here, trichlorfon was electropolymerized on the surface of the MWCNT/Ag NP–modified gold nanoelectrode with o -phenylenediamine to prepare the molecularly imprinted polymer-based sensor. After eluting the trichlorfon, imprinted holes for the identification of trichlorfon were retained on the sensor, which were used as signal switches to obtain different ECL intensities through the adsorption of different concentrations of trichlorfon. The ECL signal of the sensitized luminol–H 2 O 2 was doubly enhanced by the MWCNTs/Ag and trichlorfon, improving the sensitivity of the sensor. The trichlorfon concentration was positively correlated with the enhanced ECL intensity of the sensor in the range 5.0 × 10 −8 –5.0 × 10 −11 mol L −1 , and the detection limit of trichlorfon was 3.9 × 10 −12 mol L −1 . Moreover, the proposed sensor was successfully applied to the detection of trichlorfon residues in real samples, and the recovery ranged between 91.8 and 109%. Graphical abstract A molecularly imprinted electrochemiluminescence sensor for trichlorfon detection by utilizing the double enhancement effect of trichlorfon and Ag nanoparticles supported by multi-walled carbon nanotubes in a luminol–H 2 O 2 ECL system. The dual enhancement of the ECL signal improved the sensitivity of the sensor.
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Scientific Reports
·
2022
·Vol. 12
·期 1
·1-17
·
IF 4.996
Zhou Runjuan,Zhang Ming,Shao Shuai
The purpose of this work is to study the pyrolysis conditions of target biochar suitable for target heavy metal ion, to characterize the optimized target biochar, and to study the adsorption performance of biochar. With Cu 2+ and Zn 2+ as the target pollutants, the pyrolysis conditions involved in the preparation process as pyrolysis temperature, pyrolysis time, and heating rate were evaluated and optimized from Box–Behnken Design (BBD), response surface methodology (RSM) and desirability function, the optimized pyrolysis conditions of target biochar for Cu 2+ (Cu-BC) and Zn 2+ (Zn-BC) were obtained. The optimum pyrolysis parameters for Cu-BC and Zn-BC were pyrolysis time of 3.09 and 2.19 h, pyrolysis temperature of 425.27 and 421.97 °C, and heating rate of 19.65 and 15.88 °C/min. The pseudo-second-order kinetic and Langmuir isotherm model proved to be the best fit for the equilibrium data, with a maximum adsorption capacity ( Q max ) fitted by Langmuir model were 210.56 mg/g for Cu 2+ by Cu-BC and 223.32 mg/g for Zn 2+ by Zn-BC, which were both higher than the Q max of unoptimized biochar (BC) for Cu 2+ (177.66 mg/g) and Zn 2+ (146.14 mg/g). The physical properties, chemical structure, surface chemistry properties of Cu-BC and Zn-BC were characterized by Zeta potential meter, Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). This study puts forward a new perspective for optimizing target biochar production for special environmental application.
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