Journal of Functional Foods
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2023
·
IF 5.6
Tao Gao,Shuang Liu,Huang-yang Luo,Ren Wu,Rui Yan,Ling-zhi He,Qing-yu Nie,Hua-li Tang,Guiju Sun
Chuanminshen violaceum , as a food and folk medicine, is cultivated and grown only in China. Its polysaccharides play an important role in medicinal benefits. Herein, crude C. violaceum polysaccharides (CVPs) were purified to yield two polysaccharide fractions (CVPs-1-G and CVPs-2-G) using DEAE Cellulose-52 and Sephadex G-100 column chromatography. Structural analyses indicated that both CVPs-1-G and CVPs-2-G were glucans with average molecular weights of 8.1 kDa and 187.5 kDa, respectively. The main chain of the major component (CVPs-1-G) might be composed of (1 → 4)-linked α-Glc p , and the branched-chain might be an α-Glc p → 4)-α-D-Glc p -(1 → 6)-α-D-Glc p -(1 → 4)-α-D-Glc p , which connected to the main chain through the O-6 position of → 3,6)-α-D-Glc p -(1 →. In addition, CVPs-1-G displayed ABTS, DPPH, and hydroxyl radical scavenging activity, as well as competitive inhibitory activities of α-amylase and non-competitive inhibitory activities of lipase and pepsin. According to molecular docking, its inhibitory activity on digestive enzyme might be caused by hydrogen bond and hydrophobic interaction between them. Thus, CVPs exhibit strong potential for use in functional foods and pharmaceuticals.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 17
·5798-5803
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IF 4
Lehui Liu,Feifei Yuan,Yisheng Huang,Zhoubin Lin,Lizhen Zhang
The [B3O6] group has attracted a lot of attention as α-BaB2O4 (α-BBO) is composed of the [B3O6] group showing a short UV cut-off edge and a large birefringence. However, it is difficult to synthesize novel crystals with properties beyond α-BBO using [B3O6] as a fundamental building block (FBB). Hence, we successfully synthesized two cases of α-BBO-type organic–inorganic hybrid compounds using α-BBO as the template and melamine [C3N6H6] as the fundamental building block. The coplanar and parallel arrangement of π-conjugated [C3N6H6] groups makes them exhibit excellent optical properties, including improved birefringence responses (0.264 and 0.243@546 nm) and wide bandgaps (4.12 and 4.20 eV).
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
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IF 4.2
Xuejuan Wei,Xiaopeng Chen,Linlin Wang,Xiaojie Wei,Yang Yang,Jiaqi Liang,Jiezhen Liang
Isothermal vapor–liquid equilibrium data of the binary system (−)-α-pinene + (−)-β-pinene and the quaternary system (−)-α-pinene + (−)-β-pinene + water + phenoxyacetic acid were measured at 313.2, 323.2, and 333.2 K using headspace gas chromatography. The experimental data showed that the addition of phenoxyacetic acid and water promoted the separation of (−)-α-pinene and (−)-β-pinene. The experimental data were correlated using the UNIQUAC and NRTL models. The two models showed good correlation for the binary system, whereas the UNIQUAC model gave more accuracy for the quaternary system. Moreover, the COSMO-RS model can be an efficacious method for predicting VLE data of the determined binary and quaternary systems. In addition, the optimal spatial configuration and binding energy between the molecules were obtained by DFT, and the effects on (−)-α-pinene and (−)-β-pinene with the addition of phenoxyacetic acid and water were discussed on the molecular scale.
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Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 27
·8432-8441
·
IF 6.641
Jing Zhao,Chen Wu,Lipeng Zhai,Xiaofeng Shi,Xin Li,Guojun Weng,Jian Zhu,Jianjun Li,Jun-Wu Zhao
A surface-enhanced Raman scattering (SERS)-based sandwich type immunoassay was developed for the detection of α-fetoprotein (AFP) with high sensitivity, reproducibility and selectivity. In the immunoassay, an antibody immobilized nitrocellulose (NC) membrane was employed to capture AFP and antibody conjugated silica coated gold/silver core–shell nanostars (AuNS@Ag@SiO2) were used as SERS probes. AuNS@Ag was synthesized by the direct deposition of Ag on the AuNS. The Ag shell grew outward from the center towards the tips and adopted a polyhedral shape. The AuNS@Ag nanoparticles were shown to have higher SERS activity than gold nanostars (AuNSs), and their LSPR wavelengths could be finely tuned across a wide spectrum ranging from visible to near-infrared by simply adjusting the Ag shell thickness. The silica coating improved colloidal stability of the nanostructure and provided functionality for antibody conjugation. By using 4-mercaptobenzoic acid (4-MBA) as a Raman reporter molecule, the proposed SERS sensor is able to detect AFP with a low limit of detection (LOD) of 0.72 pg mL−1 and a wide and clinically relevant linear detection range from 3 pg mL−1 to 3 μg mL−1. Hence, this SERS-based immunoassay provides a strategy for the detection of trace AFP and has potential to assist in the monitoring of prognosis for hepatocellular carcinoma (HCC) patients.
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FUEL
·
2019
·
IF 5.128
Feifei Li,Xiaodong Wang,Hui Pan,Qiuye Li,Jianjun Yang
In this paper, disk-like α -Fe 2 O 3 nanoparticles were synthesized by one-step via an n-octyl alcohol-mediated self-assembly process and were subsequently used as the catalyst for the updating of heavy crude oil. The characterization results show that the diameter of the as-prepared α -Fe 2 O 3 disks is in the range of 150–200 nm and the nanoparticles have a hematite structure with [1 1 0] preferred orientation. The catalyst strongly acts on the asphaltene and causes its lower S content and N content, lower aromaticity and aromatic condensation, and higher branchiness index. Adding tetralin causes the increment of aromatics content, the decrease of resin, and more molecules containing aromatic moieties bearing 2 and 3 rings. The viscosity of the Shengli extra heavy oil reduced substantially from 161180 mPa∙s to 45882 mPa∙s (50 °C) through the combination of the α -Fe 2 O 3 nanoparticles and tetralin (a hydrogen donor).
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
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2019
·Vol. 34
·期 1
·47-54
·
IF 0.605
Zhang Xiaosheng,Xu Hong,Dong Jinxiang
Magnesium ion-exchanged a-zirconium phosphates (Mg- α -ZrP) with particle sizes of 600 and 80 nm were prepared through the sealed ion-exchange and one-step hydrothermal synthesis methods, respectively. It was found that larger particles of Mg- α -ZrP had a higher load-carrying capacity than that of smaller particles, whereas smaller Mg- α -ZrP particles had better anti-wear properties than that of larger Mg- α - ZrP particles under mild loads. The correlation between the particle size of the sample and the surface roughness of the friction pair thus seems to be a key factor influencing the performance.
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Biotechnology for Biofuels
·
2019
·Vol. 12
·期 1
·1-15
·
IF 5.452
Fang Wei,Xue Saisai,Deng Pengjun,Zhang Xuecheng,Wang Xiaotang,Xiao Yazhong,Fang Zemin
Background Starch is an inexpensive and renewable raw material for numerous industrial applications. However, most starch-based products are not cost-efficient due to high-energy input needed in traditional enzymatic starch conversion processes. Therefore, α-amylase with high efficiency to directly hydrolyze high concentration raw starches at a relatively lower temperature will have a profound impact on the efficient application of starch. Results A novel raw starch digesting α-amylase (named AmyZ1) was screened and cloned from a deep-sea bacterium Pontibacillus sp. ZY. Phylogenetic analysis showed that AmyZ1 was a member of subfamily 5 of glycoside hydrolase family 13. When expressed in Escherichia coli , the recombinant AmyZ1 showed high activity at pH 6.0–7.5 and 25–50 °C. Its optimal pH and temperature were 7.0 and 35 °C, respectively. Similar to most α-amylases, AmyZ1 activity was enhanced (2.4-fold) by 1.0 mM Ca 2+ . Its half-life time at 35 °C was also extended from about 10 min to 100 min. In comparison, AmyZ1 showed a broad substrate specificity toward raw starches, including those derived from rice, corn, and wheat. The specific activity of AmyZ1 towards raw rice starch was 12,621 ± 196 U/mg, much higher than other reported raw starch hydrolases. When used in raw starch hydrolyzing process, AmyZ1 hydrolyzed 52%, 47% and 38% of 30% (w/v) rice, corn, and wheat starch after 4 h incubation. It can also hydrolyze marine raw starch derived from Chlorella pyrenoidosa , resulting in 50.9 mg/g DW (dry weight of the biomass) of reducing sugars after 4 h incubation at 35 °C. Furthermore, when hydrolyzing raw corn starch using the combination of AmyZ1 and commercial glucoamylase, the hydrolysis rate reached 75% after 4.5 h reaction, notably higher than that obtained in existing starch-processing industries. Conclusions As a novel raw starch-digesting α-amylase with high specific activity, AmyZ1 efficiently hydrolyzed raw starches derived from both terrestrial and marine environments at near ambient temperature, suggesting its application potential in starch-based industrial processes.
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Microbial Cell Factories
·
2019
·Vol. 18
·期 1
·1-12
·
IF 4.402
Li Yi-Min,Gao Jiao-Qi,Pei Xu-Ze,Du Cong,Fan Chao,Yuan Wen-Jie,Bai Feng-Wu
Background l-Alanyl-l-glutamine (Ala-Gln) represents the great application potential in clinic due to the unique physicochemical properties. A new approach was developed to synthesize Ala-Gln by recombinant Escherichia coli OPA, which could overcome the disadvantages of traditional chemical synthesis. Although satisfactory results had been obtained with recombinant E. coli OPA, endotoxin and the use of multiple antibiotics along with toxic inducer brought the potential biosafety hazard for the clinical application of Ala-Gln.Results In this study, the safer host Pichia pastoris was applied as an alternative to E. coli. A recombinant P. pastoris (named GPA) with the original gene of α-amino acid ester acyltransferase (SsAet) from Sphingobacterium siyangensis SY1, was constructed to produce Ala-Gln. To improve the expression efficiency of SsAet in P. pastoris, codon optimization was conducted to obtain the strain GPAp. Here, we report that Ala-Gln production by GPAp was approximately 2.5-fold more than that of GPA. The optimal induction conditions (cultivated for 3 days at 26 °C with a daily 1.5% of methanol supplement), the optimum reaction conditions (28 °C and pH 8.5), and the suitable substrate conditions (AlaOMe/Gln = 1.5/1) were also achieved for GPAp. Although most of the metal ions had no effects, the catalytic activity of GPAp showed a slight decrease in the presence of Fe3+ and an obvious increase when cysteine or PMSF were added. Under the optimum conditions, the Ala-Gln generation by GPAp realized the maximum molar yield of 63.5% and the catalytic activity of GPAp by agar embedding maintained extremely stable after 10 cycles.Conclusion sCharacterized by economy, efficiency and practicability, production of Ala-Gln by recycling immobilized GPAp (whole-cell biocatalyst) is represents a green and promising way in industrial.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Hafiz Muhammad Saleem Akhtar,Mohamed Abdin,Yahya Saud Hamed,Wei Wang,Guijie Chen,Dan Chen,Chunxu Chen,Wei Li,Shanza Mukhtar,Xiaoxiong Zeng
In the present study, polysaccharides (CHPS) were prepared from chickpea hulls by hot water extraction and ethanol precipitation, and the physicochemical, functional, thermal and structural characteristics of CHPS were investigated. The results revealed that CHPS was comprised of mannose , rhamnose, galacturonic acid, glucose, galactose , xylose and arabinose with molar percentages of 2.16, 2.96, 42.17, 9.87, 23.15, 6.29 and 13.38%, respectively. Functional analysis demonstrated that CHPS had good fat-binding, foaming and emulsifying properties. CHPS also exhibited good thermal stability. According to X-ray spectrum, CHPS was a semi-crystalline in nature which was also confirmed from scanning electron microscopic analysis. The solid-state spectrum of 13 C NMR reflected that CHPS were complex and heterogeneous polymers. Moreover, CHPS showed favorable inhibitory activity against α-amylase. These findings indicated that CHPS could be exploited as a hypoglycemic agent or multi-purpose additive in pharmaceuticals, cosmetics and food industries.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
·
IF 3.131
Xing-An Luo,Pei Zhao,Hai Zhang,Shu-Yi Feng,Ke-Xian Chen,Zhong-Xiu Chen
Dietary macromolecules are often used for food formulation. Most of the published literature usually focuses on a certain polysaccharide reducing lipid digestion, but the non-specific physical effects such as the excluded volume effect of the surrounding macromolecules, which will possibly enhance the hydrolysis of lipids, is rarely discussed. As the influence of polysaccharides on the gastrointestinal fate of α-tocopherol acetate (VE-A) encapsulated in emulsion have not been conducted so far, in this research, we study the effect of crowded medium created by polysaccharides (Ficoll400) on the hydrolysis of VE-A and its bioaccessibility. The results show that the presence of Ficoll400 increased the bioaccessibility and conversion of VE-A 1.6 times that of a dilute buffer. An enzyme-catalyzed kinetic reaction rate of VE-A hydrolysis is increased up to 3.5 times. Both the improved thermodynamic conversion yields and kinetic rates of the hydrolysis of the VE-A emulsion was further observed in a synthetic macromolecule PEG2000 medium. This research improves our understanding of the crowding effect of macromolecules on VE-A/lipid emulsion digestion.
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BIOMATERIALS
·
2018
·
IF 8.806
Chong Zhang,Jingsong Yuan,Jianhua Lu,Yingqin Hou,Wei Xiong,Hua Lu
s The development of high-performance nonfouling polymer surfaces for implantable medical devices and therapeutic nanomaterials is of great importance. Elaborating the relationship of polymer structural characteristics and the resulted surface properties can offer useful guidance toward ideal biointerfaces . In this work, we investigate the effects of the helical conformation and anchoring orientation of poly(α-amino acid)s (PαAAs) to produce advanced nonfouling surfaces. By using the neutral poly(γ-(2-(2-(2-methoxyethoxy)ethoxy)ethoxy)esteryl glutamates) (P(EG 3 Glu)s) as a model system, the adsorption kinetics are monitored by ex-situ variable angle spectroscopic ellipsometry and in-situ quartz crystal microbalance with dissipation . It is found that the polymers adopting a rigid rod-like α-helical conformation can self-assemble more rapidly to produce denser adlayers, and generate significantly improved nonfouling surfaces compared to those flexible polymer analogues including the widely used antifouling polymer PEG . Moreover, the surface properties can be further enhanced by using the antiparallel orientated helical P(EG 3 Glu)s. Most importantly, the insights gained from the P(EG 3 Glu) model system are successfully applied to the generation of ultra-low-fouling surfaces using zwitterionic PαAAs brushes, underscoring the generality of the approach. Particularly, the surface based on the antiparallel aligned zwitterionic helical PαAAs exhibits ∼98–99% reduction of human serum adsorption relative to the bare gold, and gives almost no adhesion of mouse platelet. Taken together, this work depicts an extremely simple yet highly effective approach to manipulate surface properties for numerous applications in biomaterial interfaces, diagnostics, and biosensors.
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INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2018
·
IF 1.372
Hui Wang,Bo Qu,He Liu,Jie Ding,Nanqi Ren
A magnetic solid-phase extraction method for the preconcentration of three organochlorine pesticides (OCPs) from water samples has been proposed, based on magnetic phosphatidylcholine (MPC) as adsorbents. The extraction procedure was carried out in a single step by stirring the mixture of MPC and water samples. Subsequently, the MPC was collected by an external magnetic field without additional centrifugation or filtration. The analytes were desorbed from the MPC and finally analysed by gas chromatography–tandem mass spectrometry. The influence of various parameters on OCPs recoveries was studied. Results show that phosphatidylcholine amount and extraction time were critical in enhancing extraction performance, and the presence of humic acid was shown to significantly reduce the extraction efficiency. The limits of detection obtained were in the range of 0.1–0.15 ng L−1. Recoveries of spiked water samples ranged from 76.2% to 101.5% with relative standard deviations varying from 3.8% to 7.7%. The proposed method was employed for analysis of pentachlorobenzene, α-hexachlorocyclohexane and β-endosulfan in the surface water from two rivers in northeast China.
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危险品化学品经营许可证（带存储）