Pharmaceutics
·
2024
·Vol. 16
·期 2
·213
·
IF 4.9
Jiahui Ding,Handan Zhang,Tianli Dai,Xueqin Gao,Zhongyuan Yin,Qiong Wang,Mengqi Long,Songwei Tan
Poly (β-amino ester) (PBAE) is an exceptional non-viral vector that is widely used in gene delivery, owing to its exceptional biocompatibility, easy synthesis, and cost-effectiveness. However, it carries a high surface positive charge that may cause cytotoxicity. Therefore, hydrophilic d-α-tocopherol polyethylene glycol succinate (TPGS) was copolymerised with PBAE to increase the biocompatibility and to decrease the potential cytotoxicity of the cationic polymer-DNA plasmid polyplex nanoparticles (NPs) formed through electrostatic forces between the polymer and DNA. TPGS-b-PBAE (TBP) copolymers with varying feeding molar ratios were synthesised to obtain products of different molecular weights. Their gene transfection efficiency was subsequently evaluated in HEK 293T cells using green fluorescent protein plasmid (GFP) as the model because free GFP is unable to easily pass through the cell membrane and then express as a protein. The particle size, ζ-potential, and morphology of the TBP2-GFP polyplex NPs were characterised, and plasmid incorporation was confirmed through gel retardation assays. The TBP2-GFP polyplex NPs effectively transfected multiple cells with low cytotoxicity, including HEK 293T, HeLa, Me180, SiHa, SCC-7 and C666-1 cells. We constructed aMUC2(Mucin2)-targeting CRISPR/cas9 gene editing system in HEK 293T cells, with gene disruption supported by oligodeoxynucleotide (ODN) insertion in vitro. Additionally, we developed anLMP1(latent membrane protein 1)-targeting CRISPR/cas9 gene editing system in LMP1-overexpressing SCC7 cells, which was designed to cleave fragments expressing the LMP1 protein (related to Epstein–Barr virus infection) and thus to inhibit the growth of the cells in vivo. As evidenced by in vitro and in vivo experiments, this system has great potential for gene therapy applications.Keywords:poly (β-amino ester);gene transfection;D-α-tocopherol polyethylene glycol succinate;copolymer;CRISPR/cas9 gene editing;gene therapy
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Liang Fang,Junying Li,Hongyan Cheng,Huanhuan Liu,Caiyun Zhang
Borneol is an aromatic traditional Chinese medicine that can improve the permeability of the blood–brain barrier (BBB), enter the brain, and promote the brain tissue distribution of many other drugs. In our previous study, borneol-metformin hydrochloride water/oil/water composite submicron emulsion (B-Met-W/O/W SE) was prepared using borneol and SE to promote BBB penetration, which significantly increased the brain distribution of Met. However, the dynamic images, transport pathway (uptake and efflux), promotion of BBB permeability, and mechanisms of B-Met-W/O/W SE before and after entering cells have not been clarified. In this study, rhodamine B and coumarin-6 were selected as water-soluble and oil-soluble fluorescent probes to prepare B-Met-W/O/W dual-fluorescent SE (B-Met-W/O/W DFSE) with concentric circle imaging. B-Met-W/O/W SE can be well taken up by brain microvascular endothelial cells (BMECs). The addition of three inhibitors (chlorpromazine hydrochloride, methyl-β-cyclodextrin, and amiloride hydrochloride) indicated that its main pathway may be clathrin-mediated and fossa protein-mediated endocytosis. Meanwhile, B-Met-W/O/W SE was obviously shown to inhibit the efflux of BMECs. Next, BMECs were cultured in the Transwell chamber to establish a BBB model, and Western blot was employed to detect the protein expressions of Occludin, Zona Occludens 1 (ZO-1), and p-glycoprotein (P-gp) after B-Met-W/O/W SE treatment. The results showed that B-Met-W/O/W SE significantly down-regulated the expression of Occludin, ZO-1, and P-gp, which increased the permeability of BBB, promoted drug entry into the brain through BBB, and inhibited BBB efflux. Furthermore, 11 differentially expressed genes (DEGs) and 7 related signaling pathways in BMECs treated with B-W/O/W SE were detected by transcriptome sequencing and verified by quantitative real-time polymerase chain reaction (qRT-PCR). These results provide a scientific experimental basis for the dynamic monitoring, transmembrane transport mode, and permeation-promoting mechanism of B-Met-W/O/W SE as a new brain-targeting drug delivery system.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2025
·
IF 3.5
Zhang Yu,Xiao Xinyan,Wang Fei,Lu Ting,Cheng Xia
Photocatalytic degradation of dye wastewater has great potential due to its eco-friendly, simple, and solar energy-based characteristics. In this work, an energy-saving and convenient precipitation method was explored to compound two classical photocatalyst materials BiOBr and CAU-17. The Z-scheme heterojunction decreases the agglomeration of the BiOBr nanosheets and addresses the issue of the low photogenerated carrier participation rate of CAU-17. The solvothermal method was used to prepare CAU-17 nanorods, and then BiOBr nanosheets were grown on them at room temperature to fabricate BiOBr/CAU-17 composites. The samples were characterized by XRD, SEM, TEM, XPS, DRS, PL, EIS, and other techniques. The photodegradation performance was tested using Rhodamine B (RhB) as the model pollutant. The 80% BiOBr/CAU-17 composite (0.1 g/L) exhibited a degradation rate of 99.41% for RhB under simulated sunlight in 60 min, with an apparent rate constant of 0.0631 min⁻ 1 , which is 3.45 times faster than pure BiOBr and 315.5 times faster than CAU-17. Free radical capture and EPR tests confirmed that · O 2 ⁻ and h⁺ were the main active species in the process, and a reasonable photocatalytic mechanism is proposed. Additionally, cyclic tests are conducted to confirm the stability and durability of the composite, proving the potential of BiOBr/CAU-17 composites for efficient and sustainable photocatalytic applications.
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SENSORS
·
2020
·Vol. 20
·期 17
·4912
·
IF 3.275
Xuexue Xu,Rongbin Nie,Jingwen Huang,Li Yang
Optical fiber based immunosensors are very attractive for biomarker detection. In order to improve the sensor response, we propose a promising strategy which combines porous-layer modification of the fiber surface and streptavidin-biotin-peroxidase nano-complex signal amplification in chemiluminescent detection. Two hepatitis B antigens, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg), are used as the targets for analysis using the proposed sensor. Comparing to immunoassays using normal optical fiber sensors, the response of the present sensor is enhanced by a factor of 4.8 and 6.7 for detection of HBsAg and HBeAg, respectively. The limit-of-quantitation of the proposed method is as low as 0.3 fg/mL (0.01 fg/mL) with a wide linear response range of 3 fg/mL–150 ng/mL (0.1 fg/mL–160 ng/mL) for sensing HBsAg (HBeAg). Quantitative determination of HBsAg and HBeAg in human serum samples is performed, showing the applicability of the proposed method for biomarker detection.Keywords:optical fiber immunosensor;surface modification;signal amplification;hepatitis B surface antigen;hepatitis B e antigen
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Materials Today Bio
·
2025
·
IF 10.2
Shu-xian Liu,Jun-yu Zhang,Xiao-yan Zheng,Yong-zhong Du,Xiang-min Tong
Diffuse large B-cell lymphoma (DLBCL) is one of the most prevalent lymphomas, and can be classified as activated B-cell DLBCL (ABC-DLBCL) or germinal center B-cell DLBCL (GCB-DLBCL) according to molecular subtypes. Studies have shown that drug resistance is an important factor in its therapeutic failure. Tumor-specific drug delivery nanocarriers may be a promising strategy for the treatment of lymphoma. Herein, we developed a nanomedicine (RTX-DOX/DMF-Lip, RDDL) carrying the chemotherapeutic drugs doxorubicin (DOX) and dimethyl fumarate (DMF), and surface-modified with rituximab (RTX), which can recognize CD20 antigen when it comes into contact with the surface of a B-lymphoma cell. In the study, we found that DOX and DMF can be delivered to the tumor site precisely and demonstrate a potent synergistic anti-tumor effect. Once the nanomedicine was internalized, it induced apoptosis through ROS generation and the activation of caspase-3. Concurrently, DMF directly reduced the levels of glutathione (GSH) in tumor cells by inhibiting System Xc - , thereby resulting in impaired ROS detoxification and fostering an environment conducive to lipid peroxidation (LPO) and ferroptosis. Furthermore, the ROS generated by DOX synergistically propelled the process of ferroptosis, creating a potent therapeutic effect. These effects were validated in the GCB-DLBCL mouse model, where RDDL had a more potent killing effect compared to free drugs by striking SU-DHL-10 cells through a dual mechanism of apoptosis and ferroptosis, providing a new therapeutic strategy for the treatment of drug-resistant GCB-DLBCL.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Chengqi Feng,Anran Li,Yuxin Zhu,Yan Lei,Chenkai Jin,Juncheng Huang,Haining Na,Jin Zhu
Polluted water poses a significant threat to human health and ecological balance, necessitating the development of efficient and cost-effective water purification technologies. This study synthesized a composite photocatalytic material (CMF@Fe x O y -CN) using a microwave radiation synchrotron cooling technique. This material incorporates iron oxide-doped g-C 3 N 4 and in-situ micro-fibrillated cellulose, exhibiting high dispersion stability and magnetic recyclability. The material demonstrates a bandgap energy ( E g ) of 2.69 eV. Remarkably, 25 mg/20 mL of CMF@Fe x O y -CN (with 40 wt% Fe x O y -CN loading) achieved a 97.9 % degradation of Rhodamine B (Rh B) within 1 h. Even after 10 cycles, the degradation efficiency remained high at 89.4 %. The superoxide radical (·O 2 − ) played a critical role in Rh B degradation, facilitated by the stable dispersion of CMF. The issue of low photocatalytic efficiency, often caused by poor dispersion of photocatalysts, was addressed through the in-situ micro-fibrillation of cellulose, offering a green and economical solution. This study provides a theoretical foundation for applying photocatalysis in water purification.
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Asian Journal of Organic Chemistry
·
2025
·
IF 2.7
Xin-Yi Huang,Lan-Ying Han,Man-Yu Wang,Lu Li,Su-Yun Shi,Cheng-Hua Jin
A series of amides ( 19a – i ) and another of sulfonamides ( 21a – g ) containing the imidazo[2,1-b][1,3,4]thiadiazole moiety were synthesized and evaluated for their antimicrobial activities. Most of these compounds showed good antibacterial activities (MIC = 4–64 µg/mL). Compounds 21c (MIC = 4 µg/mL) and 21g (MIC = 4 µg/mL) exhibited strongest antibacterial activities that are comparable to that of the positive control gatifloxacin, along with low cytotoxicity (as measured against human LO2 cells) and no hemolysis, even at ultrahigh concentration (200 µM). These results indicate that these compounds show promise for further development as antibacterial agents.
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CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Xinyu Ping,Xiaoyu Pan,Bin Meng,Qingqing Yang,Wenlong Zhang
The dielectric performance of BaTiO 3 -based ceramic capacitors must be improved for further miniaturization and integration of energy storage devices. This study combines two optimization strategies, the grain refinement and high-entropy effect, to design BaTiO 3 -based high-entropy nanocrystalline ceramics: Ba(Ti 0.2 Zr 0.2 Nb 0.2 Y 0.2 Sn 0.2 )O 3 (HEO1) and Ba(Ti 0.2 Zr 0.2 Nb 0.2 Yb 0.2 Hf 0.2 )O 3 (HEO2). These ceramics were synthesized using fast hot-pressing sintering (FHPS), yielding HEO1-FHPS and HEO2-FHPS samples, both of which exhibit a pure perovskite structure, similar to that of samples fabricated via conventional solid-state sintering (CS), namely HEO1-CS and HEO2-CS. The relative densities of the HEO1-FHPS (96.18 %) and HEO2-FHPS (98.34 %) samples are higher than those of HEO1-CS (95.47 %) and HEO2-CS (95.40 %). In addition, the grain sizes of HEO1-FHPS (0.092 μm) and HEO2-FHPS (0.084 μm) are notably smaller than those of HEO1-CS (2.48 μm) and HEO2-CS (2.91 μm). The high relative density and small grain size of HEO1 and HEO2 improve their maximum permittivity, increase the Curie temperature and enhance dielectric stability. Furthermore, compared with HEO1-CS and HEO2-CS, HEO1-FHPS and HEO2-FHPS exhibit higher critical electric field strengths (359 and 389 kV/cm), greater recoverable energy densities (0.40 and 0.88 J/cm 3 ) and improved energy storage efficiencies (69.6 % and 70.4 %). These findings provide a promising approach for developing BaTiO 3 -based ceramics with enhanced dielectric performance.
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Materials Today Energy
·
2025
·
IF 8.6
Jinwu Hu,Yunqin Hu,Mingyue Li,Shiyu Hong,Shaozhen Wang,Caihong Fang
Herein, we synthesized new 3D flower-like boron doped PtCe nanoalloys formed by nanosheets with ultra-thin thickness (B-PtCe NSs) using a convenient one-pot method without any surfactants. The self-supporting 3D architecture overcomes the commonly closely packed and overlapping problems of conventional 2D nanosheets. The ultrathin feature of the petals provides large active areas, facilitating mass and electron transfer rates. Specifically, the synergistic effect between different components effectively modifies the electronic structures and upshifts the d -band center. The B-PtCe NSs therefore exhibited superior electrocatalytic performance to methanol and ethanol oxidation reactions (MOR/EOR). Specifically, the mass/specific activities of B-PtCe NSs were 2.75 ± 0.050 A·mg Pt −1 /27.58 ± 0.50 A·m −2 , which are 3.85/2.12 times higher than those of commercial Pt/C catalysts (0.71 ± 0.011 A·mg Pt −1 /12.96 ± 0.20 A·m −2 ) toward MOR, respectively. The ultrathin B-PtCe NSs also exhibited superior mass/specific activity of 2.12 ± 0.04 A·mg Pt −1 /21.11 ± 0.40 A·m −2 to EOR. Furthermore, the B-PtCe NSs exhibit higher electrocatalytic stability owing to their special morphological feature and promoted anti-CO properties. More importantly, the B-PtCe also demonstrates superior mass activities of 0.159, 0.851, 2.23, and 2.71 A·mg Pt −1 to the electrooxidation reactions of glucose, isopropanol, glycerol, and ethylene glycol. We unraveled the improved mechanism though density functional theory calculations.
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Nanomaterials
·
2018
·Vol. 8
·期 9
·697
·
IF 3.504
Jun Yang,Taiping Xie,Chenglun Liu,Longjun Xu
Dy-doped BiOCl powder photocatalyst was synthesized A one–step coprecipitation method. The incorporation of Dy3+replaced partial Bi3+in BiOCl crystal lattice system. For Rhodamine B (RhB) under visible light irradiation, 2% Dy doped BiOCl possessed highly efficient photocatalytic activity and photodegradation efficiency. The photodegradation ratio of RhB could reach 97.3% after only 30 min of photocatalytic reaction; this was more than relative investigations have reported in the last two years. The main reason was that the 4f electron shell of Dy in the BiOCl crystal lattice system can generate a special electronic shell structure that facilitated the transfer of electron from valance band to conduction band and separation of the photoinduced charge carrier. Apart from material preparation, this research is expected to provide important references for RhB photodegradation in practical applications.Keywords:Dy3+;BiOCl;photocatalyst;RhB photodegradation;doping modification
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2014
·Vol. 15
·期 4
·6897-6909
·
IF 2.339
Huanan Hu,Fangli Qiu,Anguo Ying,Jianguo Yang,Haiping Meng
A mild, efficient, and environmentally benign protocol for the synthesis of tetrahydrobenzo[b]pyran derivatives in the presence of readily accessible, biodegradable, and choline hydroxide based ionic liquid as catalyst has been established. The key features of the reported methodology include good to excellent yields of desired products, simple work-up procedure and good recyclability of catalysts, which may be a practical alternative to the existing conventional processes for the preparation of 4-Hpyrans to cater to the requirements of academia as well as industry.Keywords:pyran;choline hydroxide;ionic liquid;cost-effectiveness;biodegradability;recyclability
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2022
·Vol. 139
·期 34
·e52803
·
IF 3.057
Wei Zhang,Qingyin Wang,Gongying Wang,Shaoying Liu
The block copolymers PEF/PBAT were synthesized by copolymerizing ethylene 2,5-furandicarboxylate (EF) with totally biodegradable poly (butylene adipate-co-terephthalate) (PBAT). The copolymers' molecular chain structure, thermal properties, thermal stability, and mechanical properties were investigated, and the toughening mechanism was preliminarily studied via the micromorphology. The results revealed that the prepared block copolymers were amorphous, the thermal stability of PEF was improved, while the glass transition temperature decreased with the increase of PBAT content. PBAT was enhancing the tensile strength and elongation at break of PEF simultaneously. When the PBAT content was 25% (PEF/PBAT-25), the brittle-ductile transition occurred, with the tensile strength, Young's modulus and elongation at break of 72 MPa, 3210 MPa and 287%, respectively. The copolymers have broad application in the field of packaging materials.
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