ACS Omega
·
2023
·
IF 4.1
Zihan He,Haoying Song,Jingjing Cheng,Wenxiu Qiu,Junjie Hu,Zhenpeng Qiu,Qi Wang,Cong Chang,Guohua Zheng,Yan Meng
Selenium nanoparticles (Se NPs) have significant anticancer effects, but their poor water solubility and dispersibility limit their further applications in biomedical fields. Biomacromolecules have often been used as dispersants or stabilizers in synthesized Se NPs because they can enhance the dispersibility of Se NPs and reduce their side effects. Our previous studies reported a triple-helix β-glucan (BFP) from the fruiting bodies of black fungus, which showed a good self-assembly ability in constructing hollow nanotubes for loading metal nanoparticles. Therefore, in the present work, BFP nanotubes were designed as carriers to entrap large amounts of Se NPs in order to enhance their stability and anticancer effects. The results showed that Se NPs were successfully synthesized and loaded inside the BFP nanotubes, and the composite (BFP-Se) exhibited high stability and dispersibility due to the covalent Se–O bonds between the Se NPs and the hydroxyl groups on the BFP nanotubes. Moreover, BFP-Se showed significant effects on the proliferation, apoptosis, and cell cycle of HepG2 cells compared to those exhibited by Se NPs. The mechanism was associated with BFP, which acted as a dispersant or stabilizer, resulting in the enhanced cellular uptake of the Se NPs. BFP also activated the death receptor-mediated and mitochondria-mediated apoptotic pathways in HepG2 cells. These results suggest that BFP-Se has potential applications in biomedical fields, especially for the treatment of human liver cancers.
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Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 43
·23376-23386
·
IF 11.9
Xiaohui Niu,Rui Zhao,Yongqi Liu,Mei Yuan,Hongfang Zhao,Hongxia Li,Xing Yang,Hui Xu,Kunjie Wang
Biomimetic chiral metal organic frameworks (BioMOFs) act as chiral selectors to recognize and sense chiral molecules mimicking biological functional systems, providing a novel perspective for chiral recognition in natural processes. Here, a biological chiral metal–organic framework has been successfully prepared, namely ZnBTCHx (BTC = 1,3,5-benzenetricarboxylic acid, Hx = hypoxanthine), which has periodic large and small grooves similar to the double helix structure of DNA. The adaptability of the local shape of ZnBTCHx can facilitate specific recognition of the chiral guest. The tryptophan (Trp) enantiomer enters the main groove of ZnBTCHx as a guest, and the ZnBTCHx groove selectively recognizes L- and D-Trp because the size and shape of the Trp enantiomer match the groove of ZnBTCHx and it fits snugly into the primary groove. The way in which L-/D-Trp binds to ZnBTCHx reveals key molecular interactions that confer host–guest binding specificity, including π–π interactions as well as hydrogen bonding between chiral host and guest molecules, and spatial adaptation.
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HYDROMETALLURGY
·
2023
·
IF 4.7
Anyang Tang,Jiahe Zhang,Huazhen Cao,Huibin Zhang,Guoqu Zheng
The synthesis of refractory rhenium (Re) powders is significant for the production of rhenic products. In this work, the ultra-fine Re nanoparticles were synthesized via a combination of aqueous-phase reduction and rapid hydrogen reduction using NH 4 ReO 4 as Re source. The theoretical reduction route was determined by investigating the Eh-pH diagram of the Re -H 2 O system and conducting thermodynamic calculations. The optimal results were achieved by adjusting the experimental parameters like acid type, initial acidity, molar ratio of BH 4 − : ReO 4 − , feed rate of reductant, reaction temperature (T), and reaction time (t). The amorphous Re powders with minor oxide inclusions can be prepared under the optimized conditions of [HCl] = 1.35 mol L −1 , BH 4 − : ReO 4 − molar ratio = 5.32, feed rate = 15 mL min −1 , reaction temperature = 30 °C, and reaction time = 30 min. The maximum reduction efficiency reaches 99.5%. After annealing in hydrogen for 30 min, the crystalline Re nanoparticles with low oxygen content can be obtained. This work demonstrates the feasibility and efficacy of rapidly synthesizing ultra-fine Re nanoparticles in a solution-based approach.
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Polymers
·
2023
·Vol. 15
·期 21
·4235
·
IF 5
Mingyu Guo,Chengfeng Zhou,Yuandong Cui,Wei Jiang,Guangting Han,Zhan Jiang,Haoxi Ben,Xiaoli Yang
The catalytic conversion of cellulose to lactic acid (LA) has garnered significant attention in recent years due to the potential of cellulose as a renewable and sustainable biomass feedstock. Here, a series of Au/W-ZnO catalysts were synthesized and employed to transform cellulose into LA. Through the optimization of reaction parameters and catalyst compositions, we achieved complete cellulose conversion with a selectivity of 54.6% toward LA over Au/W-ZnO at 245 °C for 4 h. This catalyst system also proved effective at converting cotton and kenaf fibers. Structural and chemical characterizations revealed that the synergistic effect of W, ZnO, and Au facilitated mesoporous architecture generation and the establishment of an adequate acidic environment. The catalytic process proceeded through the hydrolysis of cellulose to glucose, isomerization to fructose, and its subsequent conversion to LA, with glucose isomerization identified as the rate-limiting step. These findings provide valuable insights for developing high-performance catalytic systems to convert cellulose.Keywords:cellulose conversion;lactic acid;reaction mechanism;rate-limiting step
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HYDROMETALLURGY
·
2023
·
IF 4.7
Nian Zhang,Fujian Li,Haiyang Xue,Zhongzhi Wang,Sen Qiu,Bangyu Fan,Yanyan Cheng,Xiangping Zhang
Aluminum (Al) is often associated with rare earth resources, and the removal of Al 3+ from rare earth elements (REEs) is a difficult task due to its amphoteric properties. Aluminum ions (Al 3+ ) enrich in Sm 3+ -Gd 3+ solution with the traditional 2-Ethylhexylphosphoric acid mono-2-ethylhexyl ester (P507)-kerosene-HCl system, emulsifying the phases, polluting the rare earth products and reducing the separation efficiency. A readily industrialized ionic liquid (IL) was synthesized with methyltrioctylammonium chloride ([N1888]Cl) and industrial naphthenic acid (NA) for the purification of Al 3+ from GdCl 3 solution. Under the experimental conditions, the extraction properties of diluted [N1888][NA], diluted NA and saponified NA were comparatively investigated and the separation factors ( β Al/Gd ) of Al/Gd were 23.0, 3.5 and 1.3, respectively. The effects of the salting-out agent, extraction agent, pH value and phase ratio on metal extraction efficiency were optimized. Finally, the industrial GdCl 3 feed with a high Al 3+ impurity of 889 mg/L was lowered to 5 mg/L. The removal efficiency of Al 3+ was 99.4%, and the purity of Gd 3+ was 99.998%. It provides a potential method for Al 3+ purification from rare earth solutions with advantages of high efficiency, raw materials availability and ready industrialization.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 11
·1-9
·
IF 5.7
Yao Yuying,Wang Yeru,Zhu Jingru,Guo Zhiqiang,Li Zongqiang,Li Qianru,Liu Su,Wang Yu,Yu Jinghua,Huang Jiadong
A DNA triangular prism nanomachine (TPN)–based logic device for intracellular AND-gated imaging of adenosine triphosphate (ATP) has been constructed. By using i-motif sequences and ATP-binding aptamers as logic control units, the TPN logic device is qualified to respond to the acidic environment and ATP in cancer cell lysosomes. Once internalized into the lysosome, the specific acidic microenvironment in lysosome causes the i-motif sequence to fold into a tetramer, resulting in compression of DNA tri-prism. Subsequently, the split ATP aptamer located at the tip of the collapsed triangular prism binds stably to ATP, which results in the fluorescent dyes (Cy3 and Cy5) modified at the ends of the split aptamer being in close proximity to each other, allowing Förster Resonance Energy Transfer (FRET) to occur. The FRET signals are excited at a wavelength of 543 nm and can be collected within the emission range of 646–730 nm. This enables the precise imaging of ATP within a cell. We also dynamically operate AND logic gates in living cells by modulating intracellular pH and ATP levels with the help of external drugs. Owing to the AND logic unit on TPN it can simultaneously recognize two targets and give corresponding intelligent logic judgment via imaging signal output. The accuracy of molecular diagnosis of cancer can be improved thus eliminating the false positive signal of single target-based detection. Hence, this space-controlled TPN-based logical sensing platform greatly avoids sensitivity to extracellular targets during the cell entry process, providing a useful tool for high-precision imaging of the cancer cell’s endogenous target ATP.
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CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Hongyan Chen,Zi-yang Huang,Wenjun Chen
Hydroxyapatite (HA), chitosan (CS), ZrO 2 and MgO suspensions were distributed by zonal electrophoresis (ZE), and then HA/CS/ZrO 2 /MgO composite coating on the surface of titanium substrate was fabricated by electrophoretic deposition (EPD) in a reverse electric field subsequently. An HA/ZrO 2 /MgO gradient composite coating ( Z1 ) was fabricated by calcination at 700 °C for 120 min. At the same time, another HA/ZrO 2 /MgO composite coating ( E1 ) was directly fabricated only by electrophoretic deposition for comparison, where ZrO 2 and MgO were osteogenic trace element compounds (OTECs). The morphology, structure, composition, wettability , apatite-forming ability, antibacterial efficacy of the composite coating Z1 were investigated by XRD , FT-IR, FESEM, EDS, contact angle goniometry, Lloyd's universal testing machine, electrochemical workstation, immersing in simulated body fluid, and inhibition experiments. The results showed that the contents of Ca, Zr, and Mg elements had a gradient distribution in direction of coating thickness. With the addition of ZrO 2 and MgO, the contact angle of Z1 is significantly reduced from 52.5° to 27.3°. Remarkably, Z1 has more improved properties than E1 , mainly manifested in the bonding strength of Z1 and titanium substrate up to 29.3 MPa, exceeding the standard requirements for bonding strength of HA coating implants (15 Mpa, iso13779-2) and as can be observed from cyclic voltammetry (CV) curve and Tafel polarization curve, Z1 exhibit superior electrochemical stability and strong corrosion resistance when immersed in HEPES simulated body fluid (H–SBF). In addition, after immersing in H–SBF for 24 d, the surface of the Z1 was completely covered by carbonated hydroxyapatite (CHA) and the Ca/P of the coating increased from 1.67 to 1.99. Furthermore, the antibacterial effect test of Z1 showed that the antibacterial rates of Z1 against Escherichia coli and Staphylococcus aureus reached to 98.2 % and 86.3 %, respectively.
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RSC Advances
·
2023
·Vol. 13
·期 39
·27512-27519
·
IF 3.9
Hongru Wang,Zheng Wei,Yangyang Zhao,Shidong Wang,Lili Cao,Fan Wang,Kai Liu,Yanfei Sun
Early diagnosis and treatment are of great significance for hindering the progression of brain disease. The limited effects of available treatments and poor prognosis are currently the most pressing problems faced by clinicians and their patients. Therefore, developing new diagnosis and treatment programs for brain diseases is urgently needed. Near-infrared (NIR)-light-responsive, lanthanide-doped upconversion nanoparticles (UCNPs) provide great advantages both in diagnosis and therapy. Hence, we synthesised nanoparticles comprised of a UCNPs core with surface functionalization. UCNPs@Au was used for NIR fluorescence imaging in the brain and inhibiting the growth of mouse glioma 261 (GL261) cells depending on photothermal properties. In addition, a UCNPs core and a mesoporous silica layer as the outer shell with a tannic acid–Al3+ ions (TA–Al) complex as a “gatekeeper” were used for pH-triggered doxorubicin/small interfering ribonucleic acid delivery in vitro. Based on our preliminary results, we expect to develop more multifunctional nanoscale diagnostic and therapeutic agents based on UCNPs for the diagnosis and treatment of brain diseases, including Alzheimer's disease, Parkinson's disease, and brain tumours.
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 21
·2300090
·
IF 3.1
Qiangqiang Jia,Zufan Yang,Qian Wang,Haishan Yang,Xiaofeng Tang,Hongyang Zhang,Lingling Cao,Gong Zhang
It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra-high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15 min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R 2 ≥ 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values ≤ 9.3%), and precise (the intra- and inter-day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin-intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.
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MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-9
·
IF 5.7
Huang Xuemin,Chen Haiyan,Huang Rui,Shi Yuande,Ye Ruihong,Qiu Bin
Benefit from the strong coordination property, lanthanide metal ions have been used as competitive reagents to modulate the fluorescence changes of the system. However, lanthanide metal ions as inducers for aggregation-induced emission enhancement in nanosystems is rare. Herein, we report a “turn on–off-on” fluorescent switch for cascade detection of acid phosphatase (ACP) and adenosine triphosphate (ATP) based on the competitive coordination of samarium ions (Sm 3+ ). Novel copper nanoclusters (CuNCs) with long wavelength emission (614 nm) stabilized by glutathione (GSH) and glycylglycine (Gly-Gly) have been confirmed to have AIE property. With the continuous aggregation of GSH/Gly-Gly CuNCs under the induction of Sm 3+ , the fluorescence of the system increased to achieve the “turn-on” process. The coordinated behaviour between Sm 3+ and GSH/Gly-Gly CuNCs is discussed. Due to the strong metal coordination ability of ATP, the Sm 3+ coordinated with the GSH/Gly-Gly CuNCs is competed out, resulting in the fluorescence “turn-off” process of the system. As the substrate of enzymatic hydrolysis of ACP, with the continuous hydrolysis of ATP by ACP, Sm 3+ coordinates with GSH/Gly-Gly CuNCs again, which leads to the AIE effect and realize the fluorescence "turn-on" process of the system. This strategy results in ATP linear range of 0.508 ~ 120.0 μM with a detection limit of 0.508 μM (S/N = 3) and ACP linear range of 0.011 ~ 30.0 U·L −1 with a detection limit of 0.011 U·L −1 (S/N = 3). Application to biologic samples was successful. Graphical Abstract
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Yannan Zhao,Xinwen Zhang,Chunyan Jia,Jiangling Wu,Hua Tang,Jingchuan Shang,Chuanji Yuan,Yi Wang,Pu Zhang
As a first-line tuberculostatic drug, isoniazid (INH) plays effective and irreplaceable role in prevention and treatment of tuberculosis. In this work, a rapid and simple signal-on fluorescence approach is established for INH assay by employing a platform composed of silver nanoclusters (AgNCs) and MnO 2 nanosheets. In the proposed sensing system, strong red fluorescence of poly (methacrylic acid)-stabilized AgNCs can be greatly quenched after they attach to the surfaces of MnO 2 nanosheets. With the addition of INH, MnO 2 nanosheets are reduced to Mn 2+ and subsequently release the AgNCs, which leads to obvious fluorescence recovery again. Based on this mechanism, highly sensitive detection of INH in the range of 0.8–200 μM is realized (detection limit: 476 nM). The present strategy shows remarkable advantages including simplicity, rapidness, high sensitivity and wide detectable range. This method is also practical and comparable to high-performance liquid chromatography, which can be applied to detect INH in human urine and serum samples as well as pharmaceutical products.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Qingyi Wei,Peiyao Zhang,Ting Liu,Hongbin Pu,Da-Wen Sun
As a potential carcinogen produced in food thermal processing, acrylamide (AM) can cause irreversible harm to human health. For the detection of AM in food products, a simple fluorescent biosensor based on single-stranded DNA (ssDNA) and carbon quantum dots (CQDs) was developed. Reduced fluorescence intensity of CQDs at 445 nm (excitation at 350 nm) was induced by the attachment of ssDNA. In the presence of AM, ssDNA was preferentially bound to AM by hydrogen bonding and the degree of fluorescence reduction was smaller than that without AM. Under optimized conditions, results showed that the sensing approach for detecting AM had a low detection limit of 2.41 × 10 −8 M in the standard solution, and a linear relationship ranging from 5 × 10 −3 to 1 × 10 −7 M with the determination coefficient (R 2 ) of 0.9895 was obtained. Furthermore, a good recovery percentage (91.36–98.11%) in bread crust showed the potential for practical applications of this proposed biosensor.
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