MINERALS ENGINEERING
·
2020
·
IF 3.795
Zhigang Yin,Shengda Chen,Zhijie Xu,Chenyang Zhang,Jianyong He,Jingxiang Zou,Daixiong Chen,Wei Sun
In this work, L-cysteine was introduced as an environmentally-efficient flotation reagent in the selective separation of molybdenite from chalcopyrite. Its flotation performance and adsorption mechanism onto chalcopyrite were also investigated by flotation experiment, zeta potential, FTIR spectra, Raman spectra, TOF-SIMS measurements and DFT calculations. The flotation results indicated that L-cysteine exhibited stronger depressing ability than the conventional chalcopyrite depressants of Na 2 S, NaHS and sodium thioglycolate, suggesting it could become a promising potential depressant in the selective flotation separation of molybdenite from chalcopyrite. The results of zeta potential, FTIR spectra, TOF-SIMS and Raman spectra measurements demonstrated that L-cysteine might chemisorb on the Cu atoms of chalcopyrite surface via -SH and –NH 2 groups to form a stable L-cysteine-Cu chelate compound. The DFT calculations further supported the experimental characterizations; the calculated adsorption reaction energy of L-cysteine with Cu in the (0 0 1) surface of chalcopyrite was much lower than that of L-cysteine with Fe, which implied that L-cysteine would prefer to adsorb onto the Cu site of chalcopyrite surface.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2020
·Vol. 44
·期 5
·e13186
·
IF 1.662
Ding Zhu,Xuewei Huang,Fen Tu,Cunwen Wang,Fang Yang
Black soldier fly larvae protein (BLP) was hydrolyzed using alcalase, neutrase, trypsin, and papain. The BLP hydrolysates (BLPHs) were fractionated by ultrafiltration into three peptide fractions of molecular weight (<3 kDa, 3–10 kDa and >10 kDa). Their antioxidant activities in vitro and the amino acid composition were determined. Results showed that the alcalase was more efficient in hydrolyzing the BLP into oligopeptides. BLPHs-I presented the best scavenging activity to superoxide radicals, hydroxyl radicals, DPPH, and ABTS radicals. The best scavenging activities were found in BLPHs-I containing high levels of aromatic and hydrophobic amino acids. Seventeen novel sequences with typical features of well-known antioxidant proteins were identified by LC-MS/MS. Results demonstrated that BLPHs-I possesses a great capacity as antioxidant peptides applied in functional foods. Practical applications Black soldier fly larvae protein (BLP) can also be hydrolyzed to produce antioxidant peptides and their sequences were identified. It can be used in pharmaceutical products and functional foods.
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高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Penghui Wang,Wei Liu,Shuai Liu,Rong Yang,Yajie Pu,Wenjie Zhang,Xiaoxue Wang,Xin Liu,Yanhan Ren,Bo Chi
Here, a novel pH-responsive block copolymer, poly (ethylene glycol)-poly(ε-caprolactone)-poly(L-histidine) (PEG-PCL-PHis), was synthesized and designed for anti-cancer drug delivery with excellent biocompatible, biodegradable, and strong drug loading efficiency. 1H-NMR, IF-IR, and GPC were used to characterize the structure of the PEG-PCL-PHis copolymer. In addition, the morphology, particle size, Zeta potential, and critical micelle concentration (CMC) of different degree of polymerization were determined by transmission electron microscopy (TEM), dynamic light scattering granulometer (DLS), and fluorescence spectrometer, respectively. The strong affinity between the core of micelles and hydrophobic drug was manifested with 15.09% drug loading content and 84.65% entrapment efficiency. In vitro release of DOX from the block copolymer micelle demonstrated, the PEG-PCL-PHis copolymer micelle has stable and durable drug releasing ability accompanied with pH-sensitivity. From the mechanism of cellular uptake the micelles, the pathway of drug release was captured by confocal laser scanning microscope. These experiments demonstrated the safe delivery for anticancer medicine through this novel copolymer. In conclusion, the PEG-PCL-PHis copolymer micelle has great potential to become a safe drug carrier for cancer chemotherapy.
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高影响因子
FUEL
·
2020
·
IF 5.578
Yun-Fei Wang,Ling-Ban Wang,Yang Li,Jin-Xiang Gu,Chang-Yu Sun,Guang-Jin Chen,Xiao-Hui Wang,Qing Yuan,Nan Li
Methane hydrate exploitation via depressurization for a reservoir with a bottom gas-rich zone was simulated using a large-sized three-dimensional hydrate simulator. A single vertical well system and a system with a combination of vertical and horizontal wells were employed to elucidate the different effects of horizontal and vertical wells on the exploitation process. The results showed that effect of promoting gas production using a horizontal well is not obvious for a high permeability reservoir. A large exploitation temperature drop may lead to ice blockage in the pipelines, and fracturing technology was introduced to solve this blockage problem. Experiments performed above and below the freezing point inferred that the decomposition of the hydrate in different areas of the reactor was not simultaneous, according to analysis of the temperature distribution of the entire reactor during the exploitation process. For exploitation above the freezing point, temperatures in the bottom of the reactor tend to maintain a low level and work against hydrate decomposition. However, for exploitation below the freezing point, the low-temperature area in the bottom of the reactor preferentially converts to ice. Heat release and salt precipitation promoted hydrate decomposition, and the decomposition rate was significantly accelerated.
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Food Science & Nutrition
·
2020
·Vol. 8
·期 6
·2882-2894
·
IF 1.797
Changyuan Wang,Yuchao Feng,Shu Zhang,Tianxin Fu,Yanan Sheng,Yiwei Zhang,Yingjun Jiang,Miao Yu,Liyuan Zhang,Dongjie Zhang
Metabolomic studies were carried out using gas chromatography and mass spectrometry (GC-MS) on Daohuaxiang variety rice ( Oryza sativa L.) from the Wuchang Geographical Indication Rice Protection Area in Heilongjiang Province, to investigate the effects of storage on brown rice metabolism. The data were subjected to principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and cluster analysis using software such as SIMCA. Analysis of the samples led to the identification of a total of 160 metabolites. No significant differences were found in the amount of metabolites before and after storage. A total of 31 differential metabolites were screened, and the changes in metabolite content showed a “reverse change” overall. Storage significantly changed the content of various metabolites in rice, with fatty acids impacted most significantly. Metabolic pathway analysis revealed that fatty acid biosynthesis is a key metabolic pathway in rice storage. The degradation of brown rice quality caused by storage is closely related to the composition and content of its metabolites, and that change in lipid content significantly affects brown rice quality during storage.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 43
·61061-61071
·
IF 4.223
Wang Guanghui,Wang Bing,Fan Wenzhe,Deng Nansheng
In this study, a novel slow release carrier for the controlled release of citric acid (CA), hydroxypropyl chitosan-graft-carboxymethyl-β-cyclodextrin (HPCS-g-CMCD) was synthesized by the grafting reaction of carboxymethyl-β-cyclodextrin (CMCD) with hydroxypropyl chitosan (HPCS), and the structural characteristics of HPCS-g-CMCD were confirmed by FT-IR, TGA, and NMR. Based on HPCS- g -CMCD and CA, slow release citric acid (SRCA) was prepared by a spray drying method. HPCS- g -CMCD carrier has a better slow release performance for CA compared to HPCS and CMCD, and CA release mechanism was attributed to a Fickian diffusion. Furthermore, the release behavior of uranium in contaminated soil could be effectively controlled by SRCA. The effects of SRCA on improving the phytoremediation capacity in uranium-contaminated soil were investigated using Brassica juncea , which were grown in pots containing soil with uranium at 56 mg kg −1 . After 50 days of growth, 5 mmol kg −1 of CA, SRCA I, SRCA II, and SRCA III was applied, respectively. The results showed that slow release citric acid could enhance the uptake of uranium in Indian mustard. Uranium concentration in the root with SRCA I treatment was increased by 80.25% compared to the control, and the uranium removal efficiency of the SRCA I treatment was 1.66-fold greater than that of the control. Simultaneously, the leaching loss of uranium in SRCA I-treated soil was decreased by 37.35% compared to CA-treated soil. As a promising remediation strategy, SRCA-assisted phytoremediation may provide a kind of feasible technology with low leaching risk for remediation of uranium-contaminated soils.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2021
·
IF 3.228
Jianqing Chen,Bing Shao,Jinliang Wang,Zhiqiang Shen,Honggui Liu,Shu Li
Chlorpyrifos (CPF) is an organophosphate insecticide and can cause cell death of animals. In the study, the common carp were exposed to CPF at 0 μg/L (the control group), 1.16 μg/L (the low dose group), 11.6 μg/L (the medium dose group), and 116 μg/L (the high dose group), respectively. The carp were euthanized at the 30th day and gills were collected immediately. The ultrastructural and histopathological observations showed obvious necrosis characteristics and inflammatory injury in the CPF-treated groups. CPF exposure activated the MAPK pathway, in which the mRNA and protein expressions of extracellular signal-regulated (ERK), p38 MAP kinase (p38), and c-Jun N-terminal kinase (JNK) were increased; the mRNAs and proteins of NF-κB and TNF-α were activated; and the mRNAs and proteins of necroptosis related genes were changed (the mRNA and protein expression of RIPK1 , RIPK3 , MLKL, and FADD were increased and caspase-8 was decreased) with concentration dependency. Taken together, we concluded that CPF exposure activated the MAPK/NF-κB/TNF-α pathway, promoted inflammatory injure and evoked necroptosis in common carp gills. In addition, CPF-induced inflammation and necroptosis was concentration dependency. The toxic effects of CPF on gills provided data for both aquaculture and toxicological studies.
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EUROPEAN POLYMER JOURNAL
·
2021
·
IF 4.598
Qiaohong Peng,Li Wei,Xiaoyan Zhang,Ying Wu,Khalid Mahmood,Zhengping Liu,Lisha Zhang
Ionic liquids (ILs), instead of the classical organic solvents, have shown great improvements in chemical processes. Herein, Water-insoluble alkyl- and benzyl-substituted imidazolium bis(trifluoromethanesulfonyl)imides as non-volatile and thermostable solvents were employed in the direct polycondensation of l -lactic acid (LLA). The effects of monomer concentration, reaction temperature and miscibility between the ILs and poly( l -lactic acid) (PLLA) were comprehensively investigated. Biodegradable PLLA with weight-average molecular weight as high as 132 kDa was synthesized. Compared to melt-polycondensation of LLA, far higher molecular weight could be achieved via the solution polycondensation of LLA in ILs since the viscosity of the polymer melt could be significantly reduced based on the dilution effect of ILs. Polycondensation kinetics of LLA in bulk and in an IL demonstrated that the IL could accelerate the polycondensation of LLA. Influence of lactide, the main by-product, on the polycondensations of LLA was also studied kinetically.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Dong Zhang,Luyao Zhao,Wenjuan Wang,Qian Wang,Jianlei Liu,Yuanyuan Wang,Hui Liu,Bo Shang,Xiaoliang Duan,Hui Sun
In the current study, the changes of non-starch and starch lipids in two rice varieties during storage were investigated based on untargeted lipidomic approach. A total of 395 non-starch lipids and 13 starch lipids were identified in two rice varieties. 171 lipids in Nanjing and 6 lipids in Jianzhen were significantly different in stored rice compared to those in the fresh ones. Bioinformatics analysis on these significantly different lipids revealed that the two rice varieties possessed different lipids metabolic pathways during storage. Our study provides insights into the mechanism of the changes in non-starch and starch lipids in rice during storage.
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MOLECULES
·
2021
·Vol. 26
·期 18
·5722
·
IF 4.412
Yijie Lou,Kaxi Yu,Xiajun Wu,Zhaojun Wang,Yusheng Cui,Hanxiao Bao,Jianwei Wang,Xiurong Hu,Yunxi Ji,Guping Tang
Resveratrol (RSV) and polydatin (PD) have been widely used to treat several chronic diseases, such as atherosclerosis, pulmonary fibrosis, and diabetes, among several others. However, their low solubility hinders their further applications. In this work, we show that the solubility of PD can be boosted via its co-crystallization with L-proline (L-Pro). Two different phases of co-crystals, namely the RSV-L-Pro (RSV:L-Pro = 1:2) and PD-L-Pro (PD:L-Pro = 1: 3), have been prepared and characterized. As compared to the pristine RSV and PD, the solubility and dissolution rates of PD-L-Pro in water (pH 7.0) exhibited a 15.8% increase, whereas those of RSV-L-Pro exhibited a 13.8% decrease. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine RSV, PD, RSV-L-Pro, and PD-L-Pro against lung cancer cell line A549 and human embryonic kidney cell line HEK-293 indicated that both compounds showed obvious cytotoxicity against A549, but significantly reduced cytotoxicity against HEK-293, with PD/PD-L-Pro further exhibiting better biological safety than that of RSV/RSV-L-Pro. This work demonstrated that the readily available and biocompatible L-Pro can be a promising adjuvant to optimize the physical and chemical properties of RSV and PD to improve their pharmacokinetics.Keywords:resveratrol;polydatin;L-proline;pharmaceutical co-crystal;crystal structure;solubility and dissolution rate
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Phongphat Prawang,Yongqiang Zhang,Ying Zhang,Hui Wang
Poly(ethylene glycol) (PEG), a kind of “green” medium, was used to extract artemisinin from the Chinese herb Artemisia annua L. to replace the traditional volatile and explosive solvents like petroleum ether, diethyl ether, etc. PEGs, with different molecular weights, show excellent ability for artemisinin extraction, and the extraction efficiency decreased with the increase of molecular weight. It was also demonstrated that ultrasonic irradiation could further enhance extraction efficiency compared with conventional water bath heating. Results indicate that the extraction amount of artemisinin from one gram of the leaves could reach 15.8 mg/g under the optimal conditions with ultrasonic power of 100 W, the temperature of 50 °C, time of 1 h in PEG-200, which were optimized by response surface method. Compared with Soxhlet extraction using petroleum ether (PE), the method developed here shows higher extraction efficiency (15.8 mg/g for PEG vs. 14.9 mg/g for PE process) and shorter extraction time (1 h vs. ca. 12 h). Additionally, artemisinin in the PEG solution can be recovered using resin adsorber. The results show that the recovery of artemisinin could reach almost 100% by D101 or H103 porous resin, and then the drug could be desorbed from the resin by ethanol or ethyl acetate. Moreover, the kinetic and mechanism studies of the extraction process revealed that ultrasonic irradiation could enhance mass transfer and the hydrogen bonding interactions between artemisinin and PEG further facilitate the separation of artemisinin from the leaves. This work provides an alternative process for efficient artemisinin extraction by using an environmentally benign medium with outfield intensification, which could be potentially extended to the production of other natural drugs.
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TALANTA
·
2019
·
IF 4.916
Aysha Sarfraz Rizvi,Ghulam Murtaza,Dan Yan,Muhammad Irfan,Min Xue,Zi Hui Meng,Feng Qu
l -Kynurenine (KYN) is a metabolite of the Kynurenine pathway and is a known potential marker of immune suppressant disorders and cancer. Here, we present a molecularly imprinted two dimensional (2D) Photonic crystal (PC) hydrogel sensor for the detection of L-KYN in human serum. The sensor utilizes polystyrene-based 2D PC colloidal arrays (2D PCCA) hydrogel with methacrylic acid as the functional monomer which can imprint the L-KYN template by hydrogen bonding . After removal of the template, the resulting nanocavities in the hydrogel can selectively bind and recognize L-KYN in the serum samples. The binding is selective for L-KYN, which is revealed by shrinkage of the hydrogel volume and decrease in the particle spacing that can be easily monitored through changes in the Debye diffraction ring diameter using a LASER pointer. The sensor demonstrates visible red to green color shift upon binding to L-KYN. The 2D PC sensor demonstrates the limit of detection (LOD) of 50 nM, linear relationship of particle spacing versus L-KYN concentration range (50–1000 nM) with the analytical recovery of up to 92 % in the spiked serum samples. The sensor can distinguish between L-KYN and D-KYN and is re-usable up to five times. The sensor is available for the rapid and quantitative detection of L-KYN in the human serum.
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高影响因子
危险品化学品经营许可证（带存储）