Stem Cell Research & Therapy
·
2021
·Vol. 12
·期 1
·1-13
·
IF 6.832
He Qiting,Qin Ruixi,Glowacki Julie,Zhou Shuanhu,Shi Jie,Wang Shaoyi,Gao Yuan,Cheng Lei
Background Vitamin D is important for the mineralization of bones by stimulating osteoblast differentiation of bone marrow mesenchymal stem cells (BMMSCs). BMMSCs are a target of vitamin D action, and the metabolism of 25(OH)D3 to biologically active 1α,25(OH)2D3 in BMMSCs promotes osteoblastogenesis in an autocrine/paracrine manner. Our previous study with human BMMSCs showed that megalin is required for the 25(OH)D3-DBP complex to enter cells and for 25(OH)D3 to stimulate osteoblast differentiation in BMMSCs. Furthermore, we reported that leptin up-regulates megalin in those cells. Leptin is a known inhibitor of PI3K/AKT-dependent chaperone-mediated autophagy (CMA). In this study, we tested the hypothesis that leptin acts synergistically with 25(OH)D3 to promote osteoblastogenesis in rat BMMSCs by a mechanism that entails inhibition of PI3K/AKT-dependent CMA.Methods BMMSCs were isolated from rat bone marrow (4-week-old male SD rats); qRT-PCR and western immunoblots or immunofluorescence were used to evaluate the expression of megalin, ALP, COL1A1, RUNX2, OSX, OSP, and CMA in rBMMSCs. The osteoblast differentiation was evaluated by ALP activity, ALP staining, and calcium deposition. The viability of rBMMSCs was assessed with the CCK-8 kit. Biosynthesis of 1α,25(OH)2D3 was measured by a Rat 1α,25(OH)2D3 ELISA Kit.Results The combination of leptin and 25(OH)D3 treatment significantly enhanced osteoblast differentiation as shown by ALP activity, ALP staining, and calcium deposition, the expression of osteogenic genes ALP, COL1A1, RUNX2, OSX, and OSP by qRT-PCR and western immunoblots in rBMMSCs. Leptin enhanced the expression of megalin and synthesis of 1α,25(OH)2D3 in rBMMSCs. Our data showed that leptin inhibited CMA activity of rBMMSCs by activating PI3K/AKT signal pathway; the ability of leptin to enhance 25(OH)D3 promoted osteoblast differentiation of rBMMSCs was weakened by the PI3K/AKT signal pathway inhibitor.Conclusion sOur data reveal the mechanism by which leptin and 25(OH)D3 promote osteoblast differentiation in rBMMSCs. Leptin promoted the expression of megalin by inhibiting CMA, increased the utilization of 25(OH)D3 by rBMMSCs, and enhanced the ability of 25(OH)D3 to induce osteoblast differentiation of rBMMSCs. PI3K/AKT is at least partially involved in the regulation of CMA. These data indicate the importance of megalin in BMMSCs for vitamin D’s role in skeletal health.
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PubMed
高影响因子
CATALYSIS LETTERS
·
2021
·Vol. 152
·期 6
·1719-1728
·
IF 3.186
Feng Yi,Jiang Xiaoli,Tang Lin,Lin Dunmin,Huo Yu,Xie Fengyu,Zheng Qiaoji
Abstract Exploiting highly active and robust non-noble-metal electrocatalysts for oxygen evolution reaction (OER) occupies an important position in alleviating energy crisis and maintaining social sustainability. Herein, we have grown Co(OH) 2 nanoarrays in situ on Mn-doped NiFe LDH nanosheets to construct Mn-doped NiFe LDH@Co(OH) 2 /NF (MNF@Co(OH) 2 /NF) with core–shell structure. Due to the optimized electronic structure by manganese ion doping and the high density exposure of active sites induced by cobalt hydroxide coating, the MNF@Co(OH) 2 /NF exhibits excellent OER performances, which possesses ultralow overpotentials of 193 and 244 mV at the current densities of 10 and 100 mA cm −2 separately, and possesses a small Tafel slope of 48.4 mV dec −1 . This work offers a new perspective for the rational construction of efficient OER electrocatalysts with multi-dimensional nanostructures to improve the catalytic activity. Graphic Abstract
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高影响因子
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2021
·Vol. 99
·期 1
·122-131
·
IF 2.326
Liu Shisheng,Wei Xiaohui,Lin Song,Guo Minjie
The preparation of MgO nanoparticles with relatively small sizes and highly active surface areas is still a great challenge nowadays. The principal objective of this work was to investigate and optimize the calcined conditions of MgO with smaller sizes and higher surface areas, which contributed to its better degradation ability towards paraoxon pollutants. Firstly, the aerogel Mg(OH) 2 nanosheets with an increased surface area of 349.0 m 2 /g was prepared via a combined sol–gel-hydrothermal method. Then the calcined MgO nanoparticles with different morphology, size dimensions, and specific surface areas were obtained at different calcination temperatures from 400 to 800 °C. The optimized average grain size of MgO obtained at 600 °C was achieved to 8.7 ± 3.2 nm with a relatively high surface area up to 231.4 m 2 /g. Meanwhile, such as-prepared MgO nanoparticles showed an excellent performance towards paraoxon degradation, and the maximum degradation amount of paraoxon-ethyl was up to 57.8 mg/g@90 min at room temperature. The kinetics of degradation was consistent with the pseudo-second-order model ( R 2 > 0.99). Such excellent degradation capacity was derived from more generations of superoxide radical (•O 2 − ) sites as revealed by using the nitro blue tetrazolium (NBT) as a probe. The results indicated that the MgO nanoparticles reported herein could be an effective candidate for the environmental remediation of organophosphorus toxin pollutants due to their facile scale-up, low cost, eco-friendly characteristic, and high removal efficiency.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ziwei Gao,Tao Zhang,Md. Nahid Pervez,Guojuan Qu,Wei Jin,Yaping Zhao
Materials combing catalytic and adsorption dual active sites are very important for arsenite (As(III)) due to its high mobility in water. Therefore, in this study, a bifunctional walnut-like Co-doped La(OH) 3 composites (Co-La) with independent catalytic sites and post-formed adsorption sites were synthesized via a simple hydrothermal method. Nearly 100% As(III) removal can be achieved in Co-La and peroxymonosulfate interaction systems (Co-La/PMS) within 10 min at the molar ratio of PMS to As(III) of 1.2. The results indicate that the combined Co-La adsorption system demonstrated superior performance compared to the Co-La adsorption alone. Precisely, the maximum removal capacity of the system was measured at 243.90 mg·g −1 , and the pseudo-first-order kinetic rate constant ( k 1 ) was determined to be 1.0771 min −1 . These values were significantly greater than those observed for Co-La adsorption alone. Notably, the kinetic rate constant was 239 times faster, and the removal capacity was 4.6 times higher in the combined system. The fast kinetics of As(III) elimination can be elucidated by the production of reactive oxygen species (ROS) such as S O 4 · - , •OH, O 2 · - and 1 O 2 , which expedite the conversion of As(III) into arsenate (As(V) in this particular system. Meanwhile, the increase in adsorption capacity is ascribed to the fact that PMS can drive La(OH) 3 of Co-La to transform into transition states La 2 SO 4 (OH) 4 , thus generating a new exchangeable adsorption site for As(V). A fixed membrane reactor assembled with Co-La was utilized to achieve a near-complete removal of As(III) within 3 min. The Co-La/PMS systems demonstrate exceptional effectiveness in eliminating arsenic from water across diverse real-water metrics, offering a unique perspective and technological progress in purifying water contaminated with arsenic.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Fan Wu,Xueyu Nie,Yulun Nie,Chu Dai,Xike Tian
As reported, PMS can be activated and decomposed into different reactive oxygen species such as 1 O 2 , • OH and SO 4 •- , besides, coupling of non-radical (PMS direct oxidation) and radical (SO 4 •- ) process could enhance the utilization efficiency of PMS. In this study, we proposed another strategy of 1 O 2 -based non-radical and • OH-based radical process using layered double hydrotalcite (MgAl-LDH and MgAlCu-LDH) to regulate the degradation, transformation and even mineralization of sulfamethoxazole. Compared with PMS direct oxidation , SMX removal efficiency was increased from 41.8% to 71.7% by 1 O 2 . However, there was almost no real degradation or mineralization since the ionization intermediates including C 10 H 12 N 3 O 4 S and C 7 H 9 N 3 O 5 S accumulated with reaction time according to DFT and LC-MSMS analysis. In comparison, 100% of SMX degradation was obtained via coupling 1 O 2 and • OH-radical process, in which the mineralization efficiency reached to 24.8%. Importantly, the above-mentioned intermediates of SMX were not detected and their accumulation were also significantly inhibited. Moreover, SMX degradation behavior and kinetics could be manipulated via direct oxidation , 1 O 2 and then • OH step by step, which was beneficial to enhance the PMS utilization efficiency. Finally, based on the results of zeta potential of MgAlCu-LDH, effect of solution pH on SMX degradation, the interfacial PMS activation process was responsible to regulate non-radical and radical process.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 41
·2304079
·
IF 19
Ziyun Li,Xiaoyue He,Qizhu Qian,Yin Zhu,Yafei Feng,Wentao Wan,Genqiang Zhang
Direct seawater electrolysis provides a grand blueprint for green hydrogen (H 2 ) technology, while the high energy consumption has severely hindered its industrialization. Herein, a promising active site implantation strategy is reported for Ni(OH) 2 nanowire network electrode on nickel foam substrate by Ru doping (denoted as Ru Ni(OH) 2 NW 2 /NF), which can act as a dual-function catalyst for hydrazine oxidation and hydrogen evolution, achieving an ultralow working potential of 114.6 mV to reach 1000 mA cm −2 and a small overpotential of 30 mV at 10 mA cm −2 , respectively. Importantly, using the two-electrode hydrazine oxidation assisted seawater electrolysis, it can drive a large current density of 500 mA cm −2 at 0.736 V with over 200 h stability. To demonstrate the practicability, a home-made flow electrolyzer is constructed, which can realize the industry-level rate of 1 A cm −2 with a record-low voltage of 1.051 V. Theoretical calculations reveal that the Ru doping activates Ni(OH) 2 by upgrading d -band centers, which raises anti-bonding energy states and thus strengthens the interaction between adsorbates and catalysts. This study not only provides a novel rationale for catalyst design, but also proposes a feasible strategy for direct alkaline seawater splitting toward sustainable, yet energy-saving H 2 production.
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高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
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2018
·
IF 2.179
Ting-Ting Ruan,Wen-Wen Wang,Chun-Li Hu,Xiang Xu,Jiang-Gao Mao
Two new lead(II) borate-sulfate mixed-anion compounds, namely, Pb 4 (BO 3 ) 2 (SO 4 ) and Pb 2 [(BO 2 )(OH)](SO 4 ), have been prepared by using high-temperature melt method or hydrothermal reaction . These compounds exhibit two different types of 3D structures composed of the same anionic units of BO 3 triangles and SO 4 tetrahedra which are interconnected by lead(II) cations. In Pb 4 (BO 3 ) 2 (SO 4 ), the lead(II) ions are bridged by borate anions into 3D [Pb 4 (BO 3 ) 2 ] 2+ architectures with 1D tunnels of 8-member rings along the a -axis, which are filled by the sulfate anions. In Pb 2 [(BO 2 )(OH)](SO 4 ), the lead(II) ions are interconnected by borate and sulfate anions into 2D Pb-B-O and Pb-S-O layers parallel to the ab plane, respectively, and these layers are further condensed into the 3D lead(II) borate-sulfate framework. TGA and DSC studies indicate that Pb 4 (BO 3 ) 2 (SO 4 ) is congruently melting with a melting point of 689 °C whereas Pb 2 [(BO 2 )(OH)](SO 4 ) decomposes at approximately 335 °C. UV/Vis/NIR optical diffuse reflectance spectrum measurements reveal the optical band gaps of 4.03 and 4.08 eV for Pb 4 (BO 3 ) 2 (SO 4 ) and Pb 2 [(BO 2 )(OH)](SO 4 ), respectively. Furthermore, the electronic structures of Pb 4 (BO 3 ) 2 (SO 4 ) have also been calculated.
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高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 18
·2831-2842
·
IF 4.776
Fen Zhang,Qingtao Li,Zefeng Lin,Limin Ma,Sheng Xu,Qi Feng,Hua Dong,Yu Zhang,Xiaodong Cao
Stem cells are an alternative solution for repairing bone defects. Cell migration and homing from the edge of the defect is important for new bone formation. However, the hostile ischemic environment results in limited migration, homing and survival of stem cells. In this article, ferric hydroxide (Fe(OH)3) nanoparticle-coated poly(lactic-co-glycolic acid) microspheres (FePLGA) were designed via a Pickering emulsion to facilitate stem cell homing. In the process, Fe(OH)3 nanoparticles were immobilized uniformly on the surface of FePLGA microspheres. The morphology, element analysis and Fe content of the FePLGA microspheres were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Then, these microspheres were sintered to form porous microsphere scaffolds. Recombinant human bone morphogenetic protein-2 (rhBMP-2) was immobilized on the scaffolds via a polydopamine coating to induce osteogenic differentiation. The cell viability, adhesion and migration of mouse bone marrow stromal cells (mBMSCs) cultured on different scaffolds were tested. The results indicated that the mBMSC culture on scaffolds containing Fe(OH)3 nanoparticles exhibited better proliferation, osteoblastic differentiation and migration. Moreover, the iron-containing, rhBMP-2-releasing scaffolds could enhance cell homing efficiency and promote bone formation and osteoconduction when implanted into critical size bone defect models of rabbit femur. Therefore, this new porous PLGA scaffold modified with iron and rhBMP-2 promotes bone repair by recruiting stem cells to the defect site and inside of scaffolds.
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高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2015
·
IF 3.313
Xiaojing Li,Chunmei Zeng,Guangyin Fan
Graphene-like transition metal carbide [Ti 3 C 2 X 2 (X = OH and/or F)]-supported RuNi bimetallic nanoparticles (NPs) were synthesized from the co-reduction of ruthenium chloride and nickel chloride with Ti 3 C 2 X 2 as a stabilizer and carrier. Ti 3 C 2 X 2 -supported RuNi NPs were well dispersed in aqueous solution. The as-synthesized composites were applied as catalysts in the hydrolysis of ammonia borane (AB), which is a promising solid-state hydrogen storage material for portable fuel cell application . Results indicated that the RuNi/Ti 3 C 2 X 2 catalyst was highly active for the hydrolysis of AB at room temperature , with the highest turnover frequency number of 824.7 mol H 2 ·(mol Ru·min) −1 . The activation energy for the hydrolysis of AB in the aqueous phase reached 25.7 kJ/mol, which was lower than most of the reported values.
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高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Fei Duan,Yunqin Hu,Junfeng Lin,Mingyue Li,Hao Wu,Zhiqing Cui,Caihong Fang
To develop non-noble electrocatalysts with high activity and stability for oxygen evolution reaction (OER)/urea oxidation reaction (UOR) is crucial to boost the efficiency of overall water/urea splitting reaction (OWS/OUS) for H 2 production. Herein, we synthesized novel CeO 2 /Co(OH) 2 /FeS nanosheet arrays on nickel foam (CeO 2 /Co(OH) 2 /FeS@NF) through a simple two-step hydrothermal and following solvothermal routine. In this special structure, the ultra-thin 2D nanosheet morphology can adequately offer large contact area and thus abundant of active sites, facilitating significantly the mass transfer. Furthermore, the incorporation of FeS into CeO 2 /Co(OH) 2 will not only modify the electron structure but also provide a number of phase interfaces, which can improve the inherent electron conductivity and promote the electron transport. More importantly, the rich oxygen vacancies (O v ) sites can vary the coordination of metal centers, modulating both the charge distribution and M−O bonding strength. As results, the CeO 2 /Co(OH) 2 /FeS@NF shows superior low overpotentials of 178 mV/1.30 V at 10 mA cm −2 to OER/UOR. The activity keeps its low value of 232 mV/1.375 V even when the current density was increased to as high as 300 mA cm −2 . More importantly, the electrode of CeO 2 /Co(OH) 2 /FeS@NF//CeO 2 /Co(OH) 2 /FeS@NF just requires a low cell voltage of 1.41 V to realize OUS at a current density of 10 mA cm −2 . Specifically, we also realized solar-driven H 2 production. Plenty of H 2 bubbles were continuously generated with a high speed of 600 L h −1 m −2 on the working electrode surface once the solar panel was placed under the natural sunlight.
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高影响因子
RSC Advances
·
2016
·Vol. 6
·期 98
·95740-95746
·
IF 3.289
Lili Bie,Xue Luo,Qingqing He,Daiping He,Yan Liu,Ping Jiang
Hierarchical Cu/Cu(OH)2 nanorod arrays grown on commercially available Cu foam (Cu/Cu(OH)2 NRA/CF) was prepared via a three-step strategy involving the wet chemical synthesis of Cu(OH)2 NRA/CF, a chemical reduction reaction for conversion from Cu(OH)2 NRA/CF to Cu NRA/CF, and finally galvanostatic anodization to grow Cu(OH)2 nanoparticles on Cu NRA/CF. Hierarchical Cu/Cu(OH)2 NRA/CF shows high catalytic activity towards glucose oxidation in an alkaline solution and can serve as a promising electrode material for enzyme-free glucose sensing. At an applied potential of +0.5 V, the sensor showed a broad detection range of 0.001–1.0 mM, a high sensitivity of 9.18 mA mM−1 cm−2, and a low detection limit of 0.45 μM (S/N = 3). Furthermore, the sensor exhibits excellent selectivity against common interferents and good reliability for glucose detection in human serum samples.
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高影响因子
Journal of Materials Chemistry A
·
2016
·Vol. 5
·期 6
·2629-2639
·
IF 8.262
Xin Tong,Dajun Wu,Chi Zhang,Kenan Lian,Dayuan Xiong,Shaohui Xu,Yiping Zhu,Ruijuan Qi,Rong Huang,Lianwei Wang,Paul K. Chu
Conducting the hydrogen evolution reaction (HER) in an alkaline environment using a non-precious transition metal catalyst with high efficiency is challenging. Here, we report excellent HER activity achieved using three-dimensional (3D) tetsubo-like Co(OH)2 nanorods on a macroporous electrically conductive network (MECN) synthesized by a hydrothermal method. This unique framework comprises three levels of porous structures, including a bottom-ordered MECN substrate, an intermediate layer of vertically porous Co(OH)2 nanowires with a mean diameter of 100 nm and length of about 2 μm, and outmost Co(OH)2 nanosheets (≈20 nm). The 3D array structure with a large aspect ratio provides a large specific surface area and exposes more active sites to catalyze electrochemical reactions at the electrode–electrolyte interface. Compared with Co(OH)2 nanosheets on an MECN and foamy Co(OH)2 on an MECN structure, the synthesized architecture has excellent HER catalytic reactivity, including a low potential of −69.2 mV vs. RHE, a cathodic current density of 10 mA cm−2, a small Tafel slope of 61.9 mV dec−1, a high current density, and robust catalytic stability in 1 M KOH, and is promising in HER applications.
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高影响因子
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