POLYMER BULLETIN
·
2016
·Vol. 74
·期 1
·75-90
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IF 1.371
Chen Xiuqiong,Yan Huiqiong,Shi Zaifeng,Feng Yuhong,Li Jiacheng,Lin Qiang,Wang Xianghui,Sun Wei
A novel biosensor based on electro-co-deposition of myoglobin (Mb), sodium alginate (SA), Fe 3 O 4 -graphene (Fe 3 O 4 -GR) composite on the carbon ionic liquid electrode (CILE) was fabricated using Nafion as the film forming material to improve the stability of protein immobilized on the electrode surface, and the modified electrode was abbreviated as Nafion/Mb-SA-Fe 3 O 4 -GR/CILE. FT-IR and UV–vis absorption spectra suggested that Mb could retain its native structure after being immobilized in the SA-Fe 3 O 4 -GR composite film. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry, and a pair of symmetric redox peaks appeared in the cyclic voltammograms, indicating that direct electron transfer of Mb was realized on the modified electrode, which was ascribed to the good electrocatalytic capability of Fe 3 O 4 -GR composite, the good biocompatibility of SA and the synergistic effects of SA and Fe 3 O 4 -GR composite. The electrochemical parameters of the electron transfer number ( n ), the charge transfer coefficient ( α ) and the electron transfer rate constant ( k
s ) were calculated as 0.982, 0.357 and 0.234 s −1 , respectively. The modified electrode exhibited good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) with wide linear range from 1.4 to 119.4 mmol/L, low detection limit as 0.174 mmol/L (3 σ ), good stability and reproducibility.
研究领域:
生物传感器
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电化学
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蛋白质电化学
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纳米材料
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生物相容性材料
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分子诊断
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转化医学
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Gels
·
2023
·Vol. 9
·期 3
·246
·
IF 4.6
Abid Naeem,Chengqun Yu,Weifeng Zhu,Zhenzhong Zang,Yongmei Guan
Puerarin has been reported to have anti-inflammatory, antioxidant, immunity enhancement, neuroprotective, cardioprotective, antitumor, and antimicrobial effects. However, due to its poor pharmacokinetic profile (low oral bioavailability, rapid systemic clearance, and short half-life) and physicochemical properties (e.g., low aqueous solubility and poor stability) its therapeutic efficacy is limited. The hydrophobic nature of puerarin makes it difficult to load into hydrogels. Hence, hydroxypropyl-β-cyclodextrin (HP-βCD)-puerarin inclusion complexes (PIC) were first prepared to enhance solubility and stability; then, they were incorporated into sodium alginate-grafted 2-acrylamido-2-methyl-1-propane sulfonic acid (SA-g-AMPS) hydrogels for controlled drug release in order to increase bioavailability. The puerarin inclusion complexes and hydrogels were evaluated via FTIR, TGA, SEM, XRD, and DSC. Swelling ratio and drug release were both highest at pH 1.2 (36.38% swelling ratio and 86.17% drug release) versus pH 7.4 (27.50% swelling ratio and 73.25% drug release) after 48 h. The hydrogels exhibited high porosity (85%) and biodegradability (10% in 1 week in phosphate buffer saline). In addition, the in vitro antioxidative activity (DPPH (71%), ABTS (75%), and antibacterial activity (Staphylococcus aureus,Escherichia coli, and Pseudomonas aeruginosa)indicated the puerarin inclusion complex-loaded hydrogels had antioxidative and antibacterial capabilities. This study provides a basis for the successful encapsulation of hydrophobic drugs inside hydrogels for controlled drug release and other purposes.Keywords:hydrogel;flavonoids;antioxidant;inclusion complexes;oral formulation
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高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Juxin Pei,Jun Mei,Gan Wu,Huijie Yu,Jing Xie
The active coatings supplemented with epigallocatechin gallate (EGCG) (0.16 %, 0.32 %, and 0.64 %, respectively) combined with superchilling storage (−3 ± 0.2 °C) were used to reduce hydrogen peroxide (H 2 O 2 ) content, and inhibit lipid and protein oxidations of large yellow croaker during 42 days of superchilling storage. EGCG coatings delayed lipid and protein oxidations by inhibiting the generation of H 2 O 2 , malondialdehyde (MDA) and carbonyl groups, and maintaining a higher Ca 2+ -ATPase activity and sulfhydryl content. We also observed that EGCG treatments maintained myofibrillar organized secondary structure by keeping higher α-helix content, and also stabilized tertiary structure during superchilling storage. Low-field nuclear magnetic resonance (LF-NMR) revealed that EGCG treatments might improve the association of water molecules with protein for fixed water. In addition, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM) images both showed that these treatments could delay the myofibrillar degradation of fresh fish. Overall, we report that the active coatings containing EGCG treatments protect the lipid and protein of large yellow croaker during superchilling storage.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Chunlin Chen,Guoqing Xiao,Fei Zhong,Jian Zhou,Mingtan Wang,Chunyan Chen,Zhengwei Yang,Rui Zou,Siling Liu
In this study, polyethyleneimine (PEI) was first adsorbed on the GO surface, resulting in a positively charged GO/PEI composite. Afterwards, SA and Zn 2+ were loaded on the surface of GO/PEI through a facile route to obtain an organic-inorganic hybrids (GPS@Zn). Among them, the gas phase products of SA delayed the progress of the combustion reaction, while the catalytic carbonization of zinc oxide and the high barrier effect of GO effectively improved the thermal insulation efficiency of the char layer. The fire performance test results showed that the backside temperature of GPS@Zn/EP decreased to 161.2 °C after burning for 60 min. The swelling height and swelling ratio of GPS@Zn based EP remained at the highest value compared to the other samples. In addition, the residual char content of EP loaded GPS@Zn was as high as 31.2 %, which lays the foundation for its high thermal insulation performance. The PHRR, THR, SPR and TSP values of GPS@Zn/EP were 420.2 ± 13 kW/m 2 , 13.2 ± 0.6 MJ/m 2 ,0.078 ± 0.05 m 2 /s and 1.87 ± 0.05 m 2 , respectively, which were significantly lower than the other samples, thus proving its best flame retardant and smoke suppressing properties. The carbon residue analysis confirmed that zinc oxide remained in the char layer, which was beneficial to the improvement of its thermal insulation performance and strength .
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 30
·期 9
·3890-3900
·
IF 4.705
Ge Mingliang,Li Xinxiang,Li Yueying,Jahangir Alam S. M.,Gui Yuee,Huang Yongchao,Cao Luoxiang,Liang Guodong,Hu Guoqing
In this study, the nanohybrid drug carrier were synthesized by Pickering emulsion-templated encapsulation (PETE) method to control the sustained-released properties of the nanohybrid drug carrier; magadiite-cetyltriphenyl phosphonium bromide (MAG-CTPB-KH550) and sodium alginate (NaC 6 H 7 O 6 ) was dissolved in the aqueous phase but metronidazole (C 6 H 9 N 3 O 3 ) was dissolved in the ethyl acetate (CH 3 COOC 2 H 5 ) of the oil phase; both the oil phase and the aqueous phase were mixed and dispersed to prepared organically-modified magadiite-sodium alginate (MAG–CTPB–KH550/SA) nanohybrid drug carrier. X-ray diffraction (XRD), Flourier transform infrared spectrometry (FTIR) and scanning electron microscopy (SEM) results were shown that the most of Sodium alginate (SA) were encapsulated into the MAG–CTPB–KH550 but a few of SA were intercalated into the inner space layers of MAG–CTPB–KH550, metronidazole was combined with carrier materials through physical apparent adsorption, ion exchange and electrostatic interaction. In vitro result, it was showed that the slow release was shown less than 10% content of Sodium alginate; whereas, it was reduced the initial release percentage of Metronidazole but it was extended the sustained-released time. To reach at equilibrium, the sustained-released effects of the drug carrier were prepared with 10% of Sodium Alginate for 32 h and the maximum cumulative release percentage was 93.42% for 24 h. First order model, Baker–Lonsdale model and Korsmeyer–Peppas model were fitted to study the slow-release mechanism; the correlation coefficients (R 2 ) of the three models were found over 0.9; thus, it was well described the release kinetics behavior of drug carrier composites. The slow-release mechanisms of the drug carrier were performed swelling and dissolving but the barrier effects of the lamina that were reduced the dissolution percentage of metronidazole. Graphical Abstract
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
·
IF 4.006
Peiyun Li,Jun Mei,Jing Xie
In this study, we explored the effectiveness of chitosan (CH) and sodium alginate (SA) bioactive coatings containing increasing concentrations of ε-polylysine (ε-PL) (0.1%, 0.2%, and 0.3%) combined with modified atmosphere packaging (MAP) in 60%CO 2 /5%O 2 /35%N 2 to inhibit myofibril oxidation and degradation of farmed pufferfish during a cold storage period of 18 days. Our research revealed that ε-PL-enriched CH-SA coatings and high CO 2 MAP noticeably delayed myofibril oxidation by inhibiting the formation of carbonyl groups and by keeping higher sulfhydryl content. We also observed that these treatments preserved myofibril Ca 2+ -ATPase activity, protected their organized secondary structure by retaining higher contents of α-helix and β-sheet, and also stabilised tertiary structure during cold storage. The transmission electron microscope (TEM) images showed that the distortion degree of Z-disk and M-line had become deeper and the gap between sarcomeres had expanded gradually when a comparison was carried out between fresh fish and fish at the end of the cold storage period of 18 days. However, the treated samples were still regular in appearance with distinct light I-bands, dark A-bands and Z-disk. Overall, we report that coatings containing ε-PL and high CO 2 MAP treatments appear to protect the myofibrils of farmed pufferfish during cold storage.
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Food Bioscience
·
2026
·
IF 6.2
Zixian Xia,Xiaoyan Guan,Rui Liu,Yeting Wu,Yingying Li,Mengmeng Liu,Yifeng Ding,Xiaohong Wang,Youyou Lu
Bifidobacterium faces major challenges in the gastrointestinal tract, including inactivation under acidic conditions and insufficient targeted release. In this study, three novel heterologous dual-protein complex systems were constructed by lactoferrin (LF) with α-lactalbumin (ALA), β-lactoglobulin (BLG), casein (CN). Subsequently, sodium alginate (SA) -cross-linked gel microcapsules loaded with these dual-protein complexes and B. animalis F1-7. The optimal preparation conditions for the dual-protein were as follows: ALA/LF = 1.5, 50 °C; BLG/LF = 2, 50 °C; CN/LF = 0.5, 25 °C. Incorporation of SA and endogenous emulsification method enabled efficient encapsulation of B . animalis F1-7, achieving encapsulation efficiency exceeding 90%. B. animalis F1-7@SA-(LF-ALA/BLG/CN) exhibited strong acid resistance, favorable intestinal responsiveness, and significantly improved microbial viability. The microcapsules enhanced gastric acid tolerance primarily through a spatial barrier effect, while the incorporation of dual-protein components contributed to matrix structural reinforcement and digestion-responsive release behavior. This integrated “physical protection-structural modulation” strategy also conferred excellent 28-day storage stability. In vitro experiments by mice/human feces, combined with 16S rRNA and metabolomics analysis, revealed that the composite microcapsules effectively regulated the structure of the microbial community and altered metabolite profiles (e.g. , pathways involved in nitrogen metabolism). Overall, this study provided reproducible preparation parameters and a theoretical basis for SA-based dual-protein composite encapsulation delivery systems, and offered a promising strategy to address the coordinated challenges of activity, controlled release, and storage stability of sensitive food bioactive microorganisms or nutrients.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Aizhong Jia,Chenglong Chi,Wei Dong,Xiangyu Xu,Huiliu Zhang
The extensive use of copper leads to the generation of a large amount of copper-containing wastewater, which poses a serious threat to both biological health and the environment. Therefore, recovery and reutilization of Cu from wastewater is of great practical significance for humans. In this study, the novel hierarchically ion-imprinted microsphere (H-Cu-IIP) and surface ion-imprinted microsphere (Cu-IIP) were successfully prepared from alginate/silica composite materials for the selective recovery of Cu(II). Both synthesized materials were characterized using various techniques including FT-IR, SEM, BET, TGA, and XPS, and their adsorption properties for Cu(II) were evaluated in terms of pH, adsorption kinetics, adsorption isotherm, selective adsorption, and stability. The results showed that H-Cu-IIP and Cu-IIP achieved excellent equilibrium adsorption capacities of 390.6 mg/g and 358.4 mg/g, respectively, with high selectivity and good stability, and could effectively remove Cu(II) even in actual water environments. The Langmuir model and pseudo-second-order equation can well describe adsorption isotherms and kinetics, respectively. In addition, the catalysts derived from the Cu(II)-loaded adsorbents through simple calcination exhibits excellent catalytic efficiency for photocatalytic reduction of CO 2 . These findings suggest that the proposed method is a promising approach for the selective recovery and reuse of Cu(II) from wastewater, aligning with the principles of green and sustainable practices.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Usman Muhammad,Xuan Cao,Taoyi Zhang,Wenxi Ji,Rulong Lv,Jing Chen,Yun Wei
Conductive hydrogel-based strain and pressure sensors have been extensively employed in various fields such as soft robotics and human-machine interaction. Nonetheless, it still remains challenging to synthesize a conductive hydrogel with exquisite mechanical properties, electrical conductivity and sensitivity. Herein, a novel double network nanocomposite conductive hydrogel was fabricated by using sodium alginate (SA), polyacrylamide (PAm) and copper metal nanoparticles (CuNPs) and further utilized to construct highly sensitive strain and pressure sensors. The optimized SA:PAm/CuNPs-18 hydrogel exhibited a tensile strength of 0.42 MPa, an elongation at break of 1448 %, a toughness of 3.90 MJ m −3 and an electrical conductivity of 2.4 S m −1 . Furthermore, the SA:PAm/CuNPs-18 hydrogel-based strain sensor was successfully utilized for multi-scale sensing and monitoring of the movements of elbow joint, knee joint, wrist joints, neck muscles, facial expressions and pulse of humans. In addition, the SA:PAm/CuNPs-18 hydrogel-based pressure sensor also showed great potential to detect and differentiate handwritten letters of English even at variable applied pressures and speeds. All these results indicate that the strain and pressure sensors can be integrated in wearable electronic devices, which are useful in medical observation and accurate signature recognition of humans.
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LANGMUIR
·
2020
·
IF 3.557
Qicheng Zhang,Zeqing Li,Ming Zhang,Wentao Wang,Jian Shen,Ziqiu Ye,Ninglin Zhou
Postoperative wound repair of solid tumors resection, which is afflicted by the complex tumor microenvironment (TME) and associated with the bacterial infection, is worsening and demands prompt solutions. Meanwhile, the tumor recurrence is frequently seen during the subsequent treatment due to intraoperative bleeding. For effective postoperative cancer therapy, nanoscale carriers occur as innovative and sensitive tools for monitoring the wound state, avoiding bacterial infection, and restraining tumor recurrence. Herein, a multifunctional sodium alginate (SA) hydrogel immobilizing hemoglobin (Hb) and pH-sensitive fluorescent changing carbon quantum dots (CQDs) is rationally designed. The multifunctionalization of obtained alginate@hemoglobin@CQDs hydrogel (SA@Hb@CQDs) simultaneously consists of detection, hemostasis, and chemodynamic therapy (CDT) with monitoring of wound pH based on CQDs, stanching triggered from SA hydrogel, and Fenton reaction induced by Hb. We demonstrated that SA@Hb@CQDs can stop bleeding quickly, collect wound status information in real-time, and avert bacterial infection as well as inhibit local tumor recurrence effectively. Therefore, our work provides a promising combination approach for postoperative tumor therapy.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Tingting Yang,Zhongyuan Liu,Guanghua Xia,Dawei Yu,Zhidong Liu,Yixiang Wang,Liming Zhang,Jiamei Wang
ACP voltage-modified SPI-SA nanoparticles co-encapsulated curcumin and resveratrol. • 30 kV ACP-treated nanoemulsion had optimal stability, highest EE, and superior emulsifying properties. • 30 kV ACP-nanoemulsion enhanced surimi gel strength, WHC, and network compactness. • ACP-modified nanoemulsion inhibited lipid oxidation and water migration, keeping surimi quality for 15 days. This study applied atmospheric cold plasma (ACP) at 0–40 kV to prepare curcumin- and resveratrol-loaded SPI-SA nanoemulsions and evaluated their performance in surimi gels. Moderate ACP treatment at 30 kV uniquely optimized the interfacial structure, achieving monomodal droplet size, the highest zeta potential (−56.6 + 0.62 mV) and maximum encapsulation efficiency (curcumin 89.34 ± 0.32%, resveratrol 88.33 ± 0.22%), loading capacity (curcumin 11.91 ± 0.04%, resveratrol 17.66 ± 0.04%), along with superior antioxidant activity (DPPH and ABTS radical-scavenging activities 72.66 ± 1.31% and 67.05 ± 2.21%). Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) confirmed that ACP-induced SPI unfolding, combined with SA enrichment and polyphenol anchoring, promoted a dense hydrogen-bonded interfacial film with enhanced molecular ordering, leading to excellent storage stability and emulsifying properties. In surimi gels, the 30 kV nanoemulsion acted as an optimal active filler, reinforcing the myofibrillar protein network through physical filling and interfacial cross-linking. This resulted in the highest gel strength (3090.05 ± 23.28 g·mm) and water holding capacity (81.51 ± 0.95%), lowest cooking loss (8.20 ± 0.04%) and TBARS values (0.80 ± 0.03 mg MDA/Kg), shortest T₂ relaxation times, and densest microstructure during 15-day refrigerated storage. Unmodified nanoemulsion only provided moderate improvements. Thus, 30 kV is the optimal ACP voltage for preparing stable, high-efficiency nanoemulsions and enhancing surimi gel quality, offering a theoretical basis for ACP-modified protein-polysaccharide delivery systems. Download: Download high-res image (205KB) Download: Download full-size image
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