JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Xin Shen,Lin Zhong,Liangzhi Li,Bin Zou,Hongbo Suo,Lishi Yan
In situ saccharification of lignocellulosic biomass in low-melting mixture solvents (LoMMSs) is a promising approach for biorefinery. In this study, chemical modification of cellulase coupled with immobilization was designed to improve thermal stability and LoMMS tolerance of cellulase for in situ saccharification. Cellulase was firstly chemically modified with succinic anhydride and ethylenediamine respectively to form succinylated cellulase (S-Ce) and aminated cellulase (A-Ce), then the modified cellulases were immobilized with amino-functionalized metal-organic frameworks to establish ZIF-8/A immobilized A-Ce and S-Ce. Circular dichroism, fluorescence measurements, X-ray diffraction, fourier transform infrared spectra and scanning electron microscopy were applied to characterize the modified cellulase or immobilization carrier. ZIF-8/A immobilized A-Ce presented desired thermal stability and LoMMS tolerance. Reducing sugar yields via ZIF-8/A immobilized A-Ce during in situ saccharification of bagasse maintained stably till Bet/AA concentration reached 50%, and declined relatively slowly compared with free or other immobilized cellulase when Bet/AA concentration further increased to 90%. In situ saccharification of bagasse via ZIF-8/A immobilized A-Ce in concentrated Bet/AA (50%) can be operated at a wide temperature range (50–60 °C). The corresponding reducing sugar yield with ZIF-8/A immobilized A-Ce was 58% higher than that with free cellulase in 50% Bet/AA at 60 °C.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xin Yuan,Jia-Hui Liao,Gui-Jia Du,Yi Hou,Song-Qing Hu
Kinsenoside is a rare and valuable glycoside with extensive bioactivities. However, the enzymatic synthesis of kinsenoside has been a challenging task due to the limited enzyme toolbox and unsatisfactory yield. Herein, the β-glucosidase from Dictyoglomus thermophilum (DtBGL) was heterologously expressed, purified and enzymatically characterized. The purified DtBGL was successfully immobilized on the metal-organic frameworks of UiO-66-NH 2 . The DtBGL@UiO-66-NH 2 was fully characterized using SEM, XRD, TGA and FTIR. The studies on enzymatic properties demonstrated that DtBGL@UiO-66-NH 2 exhibited increased catalytic activity and stability compared to the free DtBGL. Particularly, DtBGL@UiO-66-NH 2 could catalyze the synthesis of kinsenoside via the reverse hydrolysis reaction and the kinsenoside yield was 34.12 % under the optimized catalytic system, which was 1.9-fold higher compared with the free DtBGL. Moreover, DtBGL@UiO-66-NH 2 displayed good reusability with a kinsenoside yield of 27.02 % after reuse for 3 times. The present work not only identifies and characterizes a highly active β-glucosidase with reverse hydrolysis activity, but also proposes the immobilized enzyme as an effective catalyst for the industrial production of glycosides.
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BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Jianli Zhang,Yutong Peng,Taoze Liu,Zhanghong Wang
In this study, papermaking black liquor lignin (ULG) was directly used to produce biochar through pyrolysis. It was found that ULG has a high ash content (15.38 %), primarily consisting of Na-related minerals. During pyrolysis, the ash can reduce the activation energy of ULG (by over 6 kJ/mol), promote the development of pore structures, and enhance the graphitization of the resulting biochar through self-catalyzation. Additionally, the Na-related minerals form abundant nanowhiskers on the surface of biochar during pyrolysis, mainly composed of Na 2 CO 3 and Na 3 PO 3 , achieving self-functionalization, particularly in biochar produced at 700 °C (ULG-700). In the adsorption process, these nanowhiskers on the surface of ULG-700 act as efficient active adsorption sites. The theoretical maximum adsorption capacity for Cd(II) and Zn(II) by ULG-700 at room temperature is 639.71 and 161.27 mg/g, respectively. This study suggests that ULG can be developed as a low-cost and high-efficient heavy metal adsorbent.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-14
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IF 5.3
Deng Tingliu,Wu Wuming,Zhou Jingjing,Zeng Qin,Wang Heye,Deng Chunyan
A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs μm −2 . Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL −1 . Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Zezhou Zhao,Wei Lou,Delai Zhong,Yao Shi,Fengjiao Zhang,Linling Wang,Xiaohui Wu,Anxu Sheng,Jing Chen
Engineering black carbon (e.g. biochar) has been widely found in natural environments due to natural processes and extensive applications in engineering systems, and could influence the geochemical processes of coexisting arsenic (As V ) and Fe II , especially when they are exposed to oxic conditions. Here, we studied time-varying kinetics and efficiencies of As V immobilization by solid-phase Fe II (Fe II solid ) and Fe III (Fe III solid ) in Fe II -As V -biochar systems under both anoxic and oxic conditions at pH 7.0, with focuses on the effects of biochar surface and biochar-derived dissolved organic carbon (DOC). Under anoxic conditions, Fe II could rapidly immobilize As V via co-adsorption onto biochar surfaces, which also serves as the dominant pathway of As V immobilization at the initial stage of reaction (0–5 min) under oxic conditions at high biochar concentrations. Subsequently, with increasing biochar concentrations, Fe III solid precipitation from aqueous Fe II (Fe II aq ) oxidation (5–60 min) starts to play an important role in As V immobilization but in decreased efficiencies of As V immobilization per unit iron. In the following stage (60–300 min), Fe II solid oxidation is suppressed and leads to As V release into solutions at >1.0 g·L −1 biochar. The decreasing efficiency of As V immobilization over time is attributed to the gradual release of DOC into solution from biochar particles, which significantly inhibit As V immobilization when Fe III solid is generated from Fe II solid oxidation in the vicinity of biochar surfaces. Specifically, 4.06 mg·L of biochar-derived DOC can completely inhibit the immobilization of As V in the 100 μM Fe II system under oxic conditions. The findings are crucial to comprehensively understand and predict the behavior of Fe II and As V with coexisting engineering black carbon in natural environments.
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CONSTRUCTION AND BUILDING MATERIALS
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2025
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IF 8
Dandan Yan,Min Li
The cement solidification method serves as an effective approach for the immobilization of heavy metal-containing waste. However, Cr(VI), a highly toxic substance, is inadequately stabilized and solidified within cement pastes, posing a significant risk of leaching. This study innovatively incorporated nano-porous nitrogen-doped carbon (NC), derived from ZIF-8 through carbonization, into the cement containing heavy metals for collaborative disposal mixing with K 2 CrO 4 (HMC-Cr). Subsequently, the hydration behavior, mechanical properties, and solidification mechanism of Cr(VI) in the composite cement pastes were examined. The experimental results revealed that, compared to ZIF-8, NC demonstrated significantly greater positive impacts on the hydration kinetics, compressive strength, and Cr(VI) immobilization capabilities of HMC-Cr cement. Compared with pure HMC-Cr pastes, the 28-day compressive strength of HMC-Cr/NC increased by 16.7 %, and the leaching concentration of heavy metal Cr decreased by 52.5 %. The HMC-Cr/NC pastes exhibited superior Cr(VI) solidification capacity, attributed to the appropriate pore size, excellent nucleation effect, and reduction properties of NC. The primary immobilization forms of Cr in HMC-Cr/NC pastes included CrO 4 2- -AFm, Cr(OH) 3 , and coordination structures involving C-NH. Consequently, NC nanomaterials derived from ZIF-8 serve as effective additives for enhancing the stable immobilization of Cr(VI) in the cement solidification treatment of heavy metal-containing waste.
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APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Huaiqi Shi,Junsong Yue,Muhammad Saad Ahmed,Xiaochen Liu,Min Tu,Yunfei Shen,Yiying Hu,Liru Zhang,Jingyun Zhao,Wenjun Shao,Zhansheng Wu
Ginsenoside Rh2 is a rare but pharmacologically potent component of ginseng, which is known for its immunomodulatory, anti-tumor, and antiaging effects. However, its low natural abundance and the instability of free enzymes used in its biosynthesis hinder large scale production. To address these challenges, a magnetic nanocomposite nickel-based metal organic framework (Ni-MOF) via a mild, bovine serum albumin (BSA)-templated synthesis method was developed. This MOF was engineered to enable simultaneous purification and co-immobilization of recombinant glycosyltransferase (UGT) and sucrose synthase (SUSy), forming a dual-enzyme catalyst system BSA-Trypsin-Fe 3 O 4 /MOF@UGT&SUSy BT-Fe 3 O 4 /MOF@UGT&SUSy for the biotransformation of ginsenoside Rh2. The immobilized enzyme system BSA-Trypsin-Fe 3 O 4 /MOF (BT-Fe 3 O 4 /MOF) demonstrated high enzyme loading 176.39 mg/g and enhanced thermal, pH, and storage stability compared to free enzymes. The co-immobilized UGT-SUSy system also facilitated in situ regeneration of UDP-glucose, significantly improving catalytic efficiency. Under optimized conditions, the system achieved a Rh2 yield of 80.67 μg/mL and retained 58.01 % of the initial activity after 12 repeated cycles. This work not only highlights the potential of BT-Fe 3 O 4 /MOF in sustainable and efficient enzymatic catalysis but also offers a sustainable and scalable approach for the enzymatic synthesis of high-value nutraceuticals.
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Natural Products and Bioprospecting
·
2024
·Vol. 14
·期 1
·1-16
·
IF 4.8
Yang Ya-Ya,Jing Shun-Li,Shao Jia-Li,Chen Ji-Xuan,Zhang Wei-Feng,Wan Si-Yuan,Shen Yu-Ping,Yang Huan,Yu Wei
Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl- γ -aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β -glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products. Graphical Abstract
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JOURNAL OF CHROMATOGRAPHY A
·
2020
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IF 4.049
Yujie Zhang,Renling Lu,Ming Chen,Shouyong Zhou,Dabing Zhang,Haifeng Han,Mingliang Zhang,Hongdeng Qiu
An alternative method for efficient synthesis of urea-functionalized silanes was proposed on the basis of an N, N’ -carbonyldiimidazole-mediated acyl-transfer reaction between various amino-containing building blocks. The employment of different parent aminosilanes and alkylamines afforded an array of urea-containing silanes, which were subsequently immobilized onto silica gel to form corresponding urea-embedded alkyl stationary phases for high-performance liquid chromatography. The different substituents on the silicon core of the derivatized silane were found to significantly influence the final chromatographic behaviors. The comparative chromatographic characterization of thus-prepared silica packings with conventional octadecyl (C18) stationary phases revealed that the urea group was beneficial to suppress silanol activity towards basic probes, as well as to increase the water-compatibility of the alkyl stationary phases. The combination of a polar urea moiety and a non-polar long alkyl chain was favorable for an enhanced steric selectivity towards shape-constrained isomers. The polarizability-sensitive feature of such stationary phases made them good candidates for efficient separation of nitro-containing polar substances.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Libo Yang,Hao Fu,Lei Dong,Mingyue Ma,Yuxiang Deng,Hanyu Wu,Yuezhou Wei,Shunyan Ning,Xinpeng Wang
The effective capture of radioiodine vapor is of paramount importance in mitigating environmental radiation risks associated with spent fuel reprocessing, as well as for thyroid diagnosis and treatment. However, adsorbents used for iodine vapor capture are susceptible to the adverse effects of water vapor, leading to significant reductions in adsorption efficiency. This study presents the design and synthesis of polyimide (PI) aerogels integrated with in situ-grown SnS 2 through a hydrothermal approach. The shape of SnS 2 /PI aerogels can be adjusted via mold manipulation, while also possessing desirable mechanical strength. The SnS 2 /PI-2 aerogel exhibits a remarkable maximum adsorption capacity of 1167 mg/g in a dry environment, with only a marginal 12.4 % decrease observed in a high-humidity environment (74 % relative humidity, R.H.), as assessed by batch experiments. During application in the fixed-bed column, the SnS 2 /PI-2 aerogel demonstrates a significant adsorption capacity of 1070 mg/g at 75 °C. Monitoring conducted for 7 consecutive days post-adsorption confirms the absence of iodine release. This is attributed to the reduction of captured I 2 by the SnS 2 /PI-2 aerogel, forming the stable crystal SnI 4 , thereby preventing its release. Therefore, the SnS 2 /PI-2 aerogels exhibit excellent humidity resistance, thermal stability, and mechanical properties, rendering them highly suitable for capturing radioiodine vapor under complex conditions. This work offers insights into developing efficient aerogel adsorbents for treating radioactive iodine exhaust gases with high humidity in fixed-bed columns or decay tanks.
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Frontiers in Microbiology
·
2024
·
IF 4
Yuhan Zheng,Feiyang Sun,Siyi Liu,Gang Wang,Huan Chen,Yongxin Guo,Xiufeng Wang,Maia Lia Escobar Bonora,Sitong Zhang,Yanli Li,Guang Chen
Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable Lactobacillus bulgaricus clusters within the gel matrix. It also maintains excellent mechanical properties. It also maintains excellent mechanical properties. F127-IEA immobilized Lactobacillus bulgaricus T15 (F127-IEA-T15) can be used in cell-recycle fermentation for over 150 days from glucose and 50 days from corn stover hydrolysate, achieving high production rates of D-LA from glucose (2.71 ± 0.85 g/L h) and corn stover hydrolysate (1.29 ± 0.39 g/L h). F127-IEA-T15 enhanced D-LA production by adsorbing and blocking toxic substances present in corn stover hydrolysate that are detrimental to cellular activity.Conclusions: The newly developed hydrogels in this study provide a robust platform for large-scale extraction of D-LA from non-detoxified corn stover.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
·
IF 3.6
Yue Guoliang,Cai Zhenling,Meng Chun,Mao Yuhong
Efficient immobilized lipase systems are essential for industrial lipid modification, yet the combined effects of enzyme loading, support properties, and post-immobilization stabilization on catalytic performance remain underexplored. In this study, Thermomyces lanuginosus lipase (TLL), Candida antarctica lipase B (CalB), and their mixture (T + C) were respectively immobilized on two structurally distinct commercial supports (A & B)—differing in hydrophobicity, surface area, and pore size—at varied loading densities. Adsorption followed monolayer kinetics, with support B enabling higher enzyme up to 44.1 mg/g wet for T + C. Co-immobilized T + C systems exhibited enhanced catalytic activity compared to individual lipases, particularly in esterification and transesterification, benefiting from the complementary substrate specificities of TLL and CalB. Chemical crosslinking with chitosan and glutaraldehyde significantly reduced lipase desorption and improved thermal stability. The optimized system Cr-B-T + C-M (crosslinked support B-TLL + CalB with medium enzyme loading) efficiently catalyzed fish oil transesterification at 60 °C for 18 h, increasing the EPA + DHA content from 30.5% to 54.8%. All immobilized lipases retained over 80% of their initial catalytic activity after three reuses (60 °C and 18 h). These results highlight the synergistic benefits of co-immobilization and crosslinking on tailored supports, offering a versatile strategy for developing stable and efficient lipase biocatalysts for high-value lipid processing.
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