MOLECULES
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2024
·Vol. 29
·期 7
·1600
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IF 4.2
Wenwen Long,Mingyue You,Jieli Li,Yan Wang,Dan Wang,Xueping Tao,Li Rao,Zhining Xia,Qifeng Fu
Enhancing the pH-independence and controlling the magnitude of electroosmotic flow (EOF) are critical for highly efficient and reproducible capillary electrophoresis (CE) separations. Herein, we present a novel capillary modification method utilizing sulfonated periodate-induced polydopamine (SPD) coating to achieve pH-independent and highly reproducible cathodic EOF in CE. The SPD-coated capillaries were obtained through post-sulfonation treatment of periodate-induced PDA (PDA-SP) coatings adhered on the capillary inner surface. The successful immobilization of the SPD coating and the substantial grafting of sulfonic acid groups were confirmed by a series of characterization techniques. The excellent capability of PDA-SP@capillary in masking silanol groups and maintaining a highly robust EOF mobility was verified. Additionally, the parameters of sulfonation affecting the EOF mobilities were thoroughly examined. The obtained optimum SPD-coated column offered the anticipated highly pH-independent and high-strength cathodic EOF, which is essential for enhancing the CE separation performance and improving analysis efficiency. Consequently, the developed SPD-coated capillaries enabled successful high-efficiency separation of aromatic acids and nucleosides and rapid cyclodextrin-based chiral analysis of racemic drugs. Moreover, the SPD-coated columns exhibited a long lifetime and demonstrated good intra-day, inter-day, and column-to-column repeatability.
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BMC MICROBIOLOGY
·
2025
·
IF 4.2
Xiaofen Sun,Yikun Wang,Ao Du,Meili Dong,Yuhan Wang,Yuanzhi Zhang,Yang Zhang,Yao Huang,Xiang Huang,Yong Liu,Jingshu Ni
Background Bacterial autofluorescence plays a vital role in photodiagnosis (PD) and antimicrobial photodynamic therapy (aPDT), yet the autofluorescence properties of wound-associated bacteria and their responses to the physicochemical microenvironment, remain underexplored. Here, we investigated the bacterial autofluorescence of Acinetobacter baumannii , Escherichia coli , Klebsiella pneumoniae , and Staphylococcus aureus under various culture conditions, including different temperatures, NaCl concentrations, and pH levels found within wounds. Fluorescence imaging was employed to quantify red fluorescence intensity, while fluorescence spectrometry was used to correlate the observed fluorescence with spectral profiles, benchmarking them against coproporphyrin and protoporphyrin IX. Results Our results revealed that the selected bacteria emitted red fluorescence in vitro, consistent with their known porphyrin biosynthesis capabilities. The intensity of red fluorescence was primarily dependent on the bacterial species, growth phase, and culture conditions. Elevated culture temperature accelerated the fluorescence metabolism, whereas increasing NaCl concentrations and alkaline pH levels inhibited red fluorescence in a dose-dependent manner. Linear regression revealed a strong positive correlation between red fluorescence intensity and peak fluorescence emission when excited at 405 nm. The biosynthesis of endogenous porphyrins varied both across and within bacterial species, with distinct porphyrins produced under specific conditions. The emission spectra of the gram-positive S. aureus consistently aligned with coproporphyrin, while the gram-negative A. baumannii , E. coli , and K. pneumoniae typically displayed fluorescence peaks characteristic of protoporphyrin IX. Notably, K. pneumoniae shifted to coproporphyrin with extended culture duration or more favourable conditions, and E. coli exhibited a similar transition as the pH increased to 9. Conclusions We concluded that the local physicochemical conditions found within wounds could affect the autofluorescence and endogenous porphyrins of the bacteria, which may, in turn, have connotations for PD and aPDT. Supplementary Information The online version contains supplementary material available at 10.1186/s12866-025-04200-3.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Xiaoyong song,Zejun Lin,Guozhou Cao,Xu Zhang,Nan Wang,Xiao–kun Ouyang
Nanomedicines can overcome several limitations of traditional chemotherapeutics such as imprecise targeting and severe side effects. However, challenges remain in selective delivery, targeted accumulation, and spatiotemporal control of release. This study aims to develop a pH- and glutathione (GSH)-responsive nanodrug delivery system that integrates photothermal therapy (PTT) and chemotherapy. We designed and synthesized degradable hollow mesoporous organosilica nanoparticles (DHMONs) with tetrasulfide bonds, which were self-assembled with copper sulfide nanoparticles (CuS NPs) via surface modification. These DHMONs@CuS nanoparticles utilize the hollow structure for a large surface area and high drug-loading capacity, enabling the encapsulation of the chemotherapeutic drug doxorubicin (DOX) for synergistic photothermal-chemotherapeutic treatment. Additionally, the surface modification of these nanoparticles with hyaluronic acid (HA) facilitates active targeting of tumor cells overexpressing CD44 receptors. The system exhibits dual responsiveness to pH and GSH, aiding precise drug release within the tumor microenvironment. Following studies demonstrate that this nanosystem effectively inhibits tumor growth, showing promising prospects for application in multimodal cancer treatment.
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Foods
·
2025
·Vol. 14
·期 15
·2631
·
IF 5.1
Wanyi Sun,Qiuyu Lu,Jiajing Chen,Xinxin Fan,Shengnan Zhan,Wenge Yang,Tao Huang,Fulai Li
This study systematically evaluated the effects of pH (4–10) on the gelation properties, structural characteristics, and in vitro digestion–fermentation behavior of fish gelatin (FG, 6% (w/v)) hydrogels combined with either xanthan gum (XG, 0.07% (w/v)) or κ-carrageenan (κC, 0.07% (w/v)). The results revealed that the gel strength, hardness, and chewiness of the composite gels initially increased (pH 4–6) and subsequently decreased with rising pH levels. This trend correlated with the formation of a dense gel network structure. Furthermore, as pH increased, in vitro digestibility showed a similar pH-dependent trend, with FG–XG demonstrating superior enhancement compared to FG–κC. The addition of XG and κC resulted in increased gas production and a decreased pH during fermentation. Intestinal microbiota analysis revealed that both FG–XG and FG–κC improved the abundances ofProteobacteriaandBacteroidetewhile reducingFirmicutes. Compared to FG–XG and FG, FG–κC promoted higher levels of the generaLachnospiraceaeandBacteroides, suggesting a more favorable impact on intestinal health. These findings provide valuable insights into the pH-responsive functional properties of FG-based hydrogels and their potential applications in designing novel food matrices with enhanced nutritional and probiotic attributes.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Mengxin Huangfu,Yuankun Ren,Wei Yang,Yu Liu,Xiyu Li
Hydrogels are promising candidates for wound dressings owing to their good biocompatibility, high-water retention, and extracellular matrix-mimicking structure. However, conventional single-layer hydrogels are hard to cope with given the dynamic pH fluctuations (pH 6–8.9) of infected wounds, which exacerbate bacterial colonization and oxidative stress. Here, we engineered a multifunctional bilayer hydrogel (named PPTC) comprising a hydrophobic polydimethylsiloxane (PDMS) top layer and a polyacrylamide (PAM)-based bottom layer integrated with tannic acid (TA) and curcumin-loaded alginate microspheres (Alg@Cur). The PDMS top layer was adopted to prevent external contamination and moisture loss, while the adhesive PAM-TA-Alg@Cur (PTC) bottom layer was designed to achieve a pH-responsive therapeutic synergy. In vitro studies showed that TA exhibited a rapid release (76.66% within 12 h) to suppress bacterial proliferation and inflammation, while Alg@Cur could slowly release curcumin in a pH-dependent manner (90.32% at pH 8.5 vs 38.06% at pH 6, cumulatively), targeting alkaline microenvironments to mitigate oxidative stress. Their synergy demonstrated potent antibacterial activity (≥99.90% inhibition against Staphylococcus aureus and Escherichia coli) and reactive oxygen species scavenging ability (93.89% DPPH elimination). In a rat-infected wound model, the PPTC bilayer hydrogel synergistically accelerated infected wound healing by reducing inflammation, enhancing collagen deposition, and promoting angiogenesis. This work pioneers a pH-driven therapeutic platform for advancing smart dressings for infected wound management.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yu Zhao,Huangmei Zhou,Longliang Qiao,Jinming Xu,Rodolphe Antoine,Lunhua Deng,Sanjun Zhang,Haifeng Ye
Precise measurements of tumor pH is essential for early cancer diagnose, treatment planning, and the development of anti-cancer drugs. Herein, we present a pH-sensitive, ratiometric fluorescent biosensor with tumor-targeting capabilities for real-time in vivo tumor pH detection. The biosensor, composed of glutathione-protected gold nanoclusters (AuNCs) conjugated with fluorescein-isothiocyanate (FITC), provides a reliable ratiometric fluorescence signal response to pH. The cyclic arginine-glycine-aspartic acid (c(RGDyC)) peptide was integrated into the sensor (denoted as c(RGDyC)-FITC@AuNCs) to confer tumor-targeting specificity. Upon excitation at 488 nm, c(RGDyC)-FITC@AuNCs exhibited dual-channel emissions at 515 nm and 710 nm, the ratio of which showed a 17-fold change across a pH range of 5.5 to 8.0. In addition, c(RGDyC)-FITC@AuNCs demonstrated excellent biocompatibility, long-term photostability, and low toxicity, enabling the quantitative monitoring of the pH changes during tumor cell apoptosis in real-time. Moreover, using tumor-bearing mouse models with induced acid-alkaline variations, c(RGDyC)-FITC@AuNCs successfully visualized and dynamically tracked in situ tumor pH. This biosensor offers a sensitive and accurate tool for tumor diagnosis, with significant potential in biomedical research and clinical studies.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Yang Liu,Xiaowen Zhang,Fashuai Yang,Cuili Xing,Peng Fan,Yuankui Sun,Xiaohong Guan
Zero-valent aluminum (ZVAl) shows promise for environmental remediation, yet its decontamination performance under circumneutral conditions remains debated. To unravel this paradox, we employed Se(IV) and nitrate (NO 3 − ) as model pollutants to probe how contaminant-specific modulation of Al 2 O 3 transformation dictates ZVAl performance across pH 0 2.0–11.0. Results revealed that Se(IV) sequestration exhibited U-shaped kinetics with minimum efficiency at pH 0 4.0–7.0 (5.8–14.2 %), whereas NO 3 − reduction displayed an inverted U-shaped trend with maximum efficiency (> 95 %) obtained in the same pH range. Mechanistic studies demonstrated that these contrasting trends originate from pollutant-mediated Al 2 O 3 evolution. Specifically, Lewis-basicity-dependent competition for Al 2 O 3 surface sites dictates whether hydration or dissolution dominates activation. Se(IV), acting as a strong Lewis base, inhibited Al 2 O 3 hydration by competing with OH − for Al 2 O 3 surface sites and/or forming passivating Al-O-SeO 3 2− complexes , thereby self-limiting its own removal. Conversely, NO 3 − with weak Lewis basicity negligibly affected Al 2 O 3 hydration to form porous Al(OH) 3 , which can enhance electron transfer by reducing interfacial charge transfer resistance. This work resolves the long-standing pH limitation paradox in ZVAl systems by elucidating the role of Al 2 O 3 evolution, thereby offering a mechanistic framework for the design of adaptive metal-based remediation technologies.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhechen Fan,Junqiu Ye,Sijia Yao,Zhongsheng Ji,Weisong Li,Jianping Zhou,Hao Cheng,Yang Ding
The efficient tumor ablation strategy accompanied with metastasis blocking is critical for triple-negative breast cancer (TNBC) therapy. Herein, we develop a cascade pH-responsive chemo-gene delivery nanosystem to actuate PANoptosis of tumor cells and suppress metastasis via bystander effect. Structurally, a charge reversal and acid-activated cell-penetrating peptide (CACPP) is covalently anchored onto GO surface, together with co-loading of doxorubicin (Dox) and TNBC-selective TRAIL expressing plasmid (pbTRAIL) onto GO surface via π-π stacking and calcium phosphate (CaP) biomineralization to yield nanosystem of Dox GPCaP pbTRAIL . After intravenous administration, the nanosystem could selectively accumulate at tumor site since CACPP modified nanosheet resurrects in tumor acidic microenvironment (Cascade 1). The internalized Dox GPCaP pbTRAIL nanosystem is further translocated into lysosome, where lower pH triggers burst drug release via Dox protonation and CaP dissolution (Cascade 2), followed with pbTRAIL liberation and lysosomal escape. The combination of Dox and TRAIL induces advanced apoptosis and necrosis of tumor cells; Meanwhile, GO-derived nanosheets share high photothermal conversion efficiency upon laser irradiation, thereby inducing pyroptosis and promoting PANoptosis synergistically. Our study demonstrates tumor-specific accumulation of Dox GPCaP pbTRAIL nanosystem and high capability of PANoptosis induction both in vitro and in vivo, yielding high tumor inhibition rate of 96 % and survival rate of 100 %. Collectively, the cascade pH-responsive multi-drug delivery nanosystem provides a novel approach for PANoptosis therapy of TNBC.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Haoran Jiang,Yong Xiao,Zhirang Liu,Zichen Wang,Bojian Wei,Qiliang Wei,Niancai Cheng
The development of high-performance electrocatalysts for hydrogen evolution reaction (HER) in different pH conditions is pivotal in producing green hydrogen, but remains challenging. Herein, we regulate the p-d orbitals hybridization between B and Pt for effective and durable HER at all pH ranges by controlling the inserted B atom. Consequently, the optimized B-doped Pt catalysts with 20 at.% B content (Pt80B20/C) has the highest HER performance, with only 7 mV overpotential in acidic conditions, 37 mV in alkaline media, and 47 mV in neutral media, more remarkably, have negligible attenuation during electrolysis up to 100 h, which is superior to commercial Pt/C catalysts. Theoretical calculations revealed that by inserting appropriate B atoms in the interstitial vacancies of Pt, the electronic structure of Pt is suitable for providing appropriate hydrogen intermediates (H*) adsorption/desorption strength, resulting in superior acid HER electrocatalyst performance. Besides, a strong electronic interaction existed between Pt and inserted-B atoms leaving Pt sites in an electron deficiency state, which facilitates the bond cleavage of the H-OH of H 2 O, hence accelerating water dissociation and promoting neutral/alkaline HER dynamics.
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Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Chenchen Li,Xintong Zhang,Weiye Xiu,Mingfu Yuan,Jingyang Wang,Diaomei Ma,Chunran Han
Astaxanthin (AST) bioavailability is inherently limited by poor stability. In this study, pH/glutathione (GSH) dual-responsive carboxymethyl chitosan (CMCS) superparamagnetic iron oxide (SPION) nanoparticles were prepared, loaded with AST (CMCS-SPION-AST), and characterized. Its stability, responsive release, and kinetics were studied. The results showed that CMCS-SPION successfully encapsulated AST. The composite showed excellent stability (88.66 % AST retention at 4 °C, light-protected) and the highest cumulative release (82.11 % at pH 5.5/10 mM GSH), which was higher than the release rates of previously reported single pH-responsive carriers. The release curves of CMCS-SPION-AST at pH 5.5, pH 7.4/10 mM GSH, and pH 5.5/10 mM GSH showed good model fit (R 2 > 0.9), indicating non-Fickian diffusion. The release process represents a synergistic effect of diffusion and dissolution. This study provides novel insight for the development of responsive drug delivery systems and expands the application prospects of AST as a functional natural pigment in the food industry.
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DYES AND PIGMENTS
·
2024
·
IF 4.1
Xingyun Gao,Jiaguang Meng,Yongzhen Wang,Yaming Liu,Chao Zhi,Lu Yang,YuQing Chen,Chao Wang
Relative humidity (RH), temperature, and pH as important environmental parameters that are closely related to industry, agriculture, and people's life. Therefore, the development of a colorimetric sensor that can monitor the RH, temperature, and pH in real-time is highly desired. In this work, a fabric-based flexible colorimetric sensor is easily manufactured by screen printing the mixture of cresol red (CR), thymol blue (TB) and boric acid (H 3 BO 3 ) on the fabric. Compared with the traditional single stimulus colorimetric sensor, this fabric device can respond to multiple stimulus factors including humidity, temperature, or pH. The simple coating treatment can endow the colorimetric sensor with fast response without compromising its flexibility. Exposed to different stimulus conditions, the colorimetric sensor has a unique color change effect, such as humidity-responsive pink-yellow, temperature-responsive yellow-pink, and pH-responsive pink-purple. The flexibility and versatility of the fabric sensors are showcased with the applications in temperature/humidity/pH detection, cultural relics protection and information encryption. We believe the colorimetric sensor would have potential in environmental monitoring, health care, encrypted information patterns, and intelligent wearables, etc.
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International Journal of Nanomedicine
·
2020
·
IF 5.115
Dan-Li Hao,Ran Xie,Ge-Jing De,Hong Yi,Chen Zang,Mi-Yi Yang,Li Liu,Hai Ma,Wei-Yan Cai,Qing-He Zhao,Feng Sui,Yan-Jun Chen
Purpose In this study, pH-sensitive poly(2-ethyl-2-oxazoline)-poly(lactic acid)-poly(β-amino ester) (PEOz-PLA-PBAE) triblock copolymers were synthesized and were conjugated with an antimalaria drug artesunate (ART), for inhibition of a colon cancer xenograft model. Methods The as-prepared polymer prodrugs are tended to self-assemble into polymeric micelles in aqueous milieu, with PEOz segment as hydrophilic shell and PLA-PBAE segment as hydrophobic core. Results The pH sensitivity of the as-prepared copolymers was confirmed by acid-base titration with p K b values around 6.5. The drug-conjugated polymer micelles showed high stability for at least 96 h in PBS and 37°C, respectively. The as-prepared copolymer prodrugs showed high drug loading content, with 9.57%±1.24% of drug loading for PEOz-PLA-PBAE-ART4. The conjugated ART could be released in a sustained and pH-dependent manner, with 92% of released drug at pH 6.0 and 57% of drug released at pH 7.4, respectively. In addition, in vitro experiments showed higher inhibitory effect of the prodrugs on rodent CT-26 cells than that of free ART. Animal studies also demonstrated the enhanced inhibitory efficacy of PEOz-PLA-PBAE-ART2 micelles on the growth of rodent xenograft tumor. Conclusion The pH-responsive artesunate polymer prodrugs are promising candidates for colon cancer adjuvant therapy.
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