MATERIALS & DESIGN
·
2025
·
IF 7.9
Wenxuan zhao,Yuzhe Chen,Xiaopei Wu,Xianzheng Dong,Honglian Dai
Peripheral nerve injury repair is hindered by an imbalanced regenerative microenvironment involving inflammation, oxidative stress, and inadequate blood supply. Traditional drugs are limited by poor blood-nerve barrier penetration and lack of multifunctionality. Nitric oxide (NO) is crucial for axonal growth, synaptic plasticity, and neuroprotection, offering anti-inflammatory, antioxidant and pro-angiogenic potential. However, uncontrolled NO release causes toxicity. To leverage NO therapeutically, sustained delivery is essential. This study developed a multifunctional nerve graft using a modified chitosan hydrogel grafted with RGDC peptide, featuring an internal orientation microstructure. Glutathione was incorporated as a precursor for the NO donor S-nitrosoglutathione (GSNO). This system promotes cell adhesion/migration and reduces oxidative stress. Results show the hydrogel is non-cytotoxic and synergistically enhances cellular adhesion. GSNO loading significantly reduces cellular reactive oxygen species (ROS) levels in human umbilical vein endothelial cells (HUVECs), facilitating their migration and angiogenesis. The GSNO-incorporated neural scaffold achieved sustained NO release and demonstrated significant antioxidant capacity. This innovative approach presents a promising strategy for clinical application in peripheral nerve injury repair.
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Discover Food
·
2025
·Vol. 5
·期 1
·1-15
·
IF 3.7
Tian Zhen,Jin Huaizhou,Shang Xiaoxiao,Ma Qifei,Wang Weihong,Jin Shangzhong
The long-term stability of Pickering emulsions is crucial for their application in food systems. This study aimed to evaluate the stability degradation of oil-in-water (O/W) Pickering emulsions stabilized by whey protein isolate (WPI) and chitosan (CS), and to assess their applicability in reduced-fat mayonnaise formulations. Optical tweezers were employed to quantify the interaction forces between emulsion droplets during storage, in combination with microstructural observation and ζ-potential analysis. It was found that the O/W emulsion prepared with a 50% aqueous phase volume fraction maintained a stable droplet size over the storage period without significant changes. The ζ-potential decreased from 79.16 mV to 53.62 mV, while the maximum droplet contact force gradually decreased from 69 pN to 37 pN by day 45. By day 60, droplet coalescence was observed. Compared with traditional rice bran oil mayonnaise, the mayonnaise formulated using this Pickering emulsion exhibited superior mechanical stability and resistance to destabilization. In particular, the formulation with a Pickering emulsion-to-egg yolk volume ratio of 4:1 showed the best mechanical performance, with the droplet contact force gradually decreasing from 68 pN on day 0 to 62 pN on day 20. This study provides a rapid method for evaluating the stability of food systems, offering potential applications in the food industry.
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ACS Nano
·
2025
·
IF 16
Zhijie Zhao,Yingying Meng,Yao Shu,Yunfeng Yi,Junjie Li,Yuping Wei
The bone fixation implants in the clinic are prone to inflammation and thrombosis that result in efficacy and patient health risks. This paper introduces chitosan microsphere-enhanced poly(sulfobetaine) (CSM/PSBMA) zwitterionic hydrogel coated on the surface of polylactic acid (PLA) fracture fixation devices through interfacial autocatalytic polymerization and in situ gelation. CSM as a disperse phase improves both the mechanical strength and swelling resistance of the PSBMA matrix of the continuous phase. The CSM/PSBMA hydrogel coating keeps the superhydrophilicity and excellent antifouling properties of the pure PSBMA hydrogel coating. In particular, the CSM/PSBMA hydrogel coating still exhibits good stability after 30 days of PBS immersion, 7 days of PBS shearing, 30 min of PBS ultrasonication, and 30 min of water flushing. Furthermore, the CSM/PSBMA hydrogel-coated PLA implants can alleviate foreign body reaction and accelerate the bone repair in the rat femoral fracture model. This brings a promising path for devising zwitterionic hydrogel coatings for implantable medical devices.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Rongxia Tang,Lu Zheng,Shangfei Chi,Lu Wei,Xiaoying Wang,Xiao Ma
Anticoagulant therapy substantially increases the risk of post-extraction hemorrhage by impairing hemostasis. In this study, we designed a quaternized carboxymethyl chitosan/polydopamine composite hemostatic sponge (QCD) that combines mechanical robustness and biocompatibility. Compared with the Gel group, QCD exhibits markedly enhanced in vitro hemostatic and antibacterial performance; the quaternary ammonium modification confers potent antibacterial activity tailored to the complex oral environment and mitigates infection risk. In vivo, tooth extraction test in anticoagulated rats showed that QCD (blood loss 0.011 ± 0.001 g, hemostasis time 59.667 ± 4.163 s) significantly outperformed a commercial gelatin sponge (0.019 ± 0.001 g, 87.000 ± 4.082 s) in hemostasis. This superiority was attributable to its rapid blood absorption and enhanced adhesion of blood cells and platelets, which together promoted clot formation. These results suggest that QCD may serve as a practical and promising approach for managing post-extraction hemorrhage in anticoagulated patients.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Feili Li,Ge Yu,Yuhan Li,Jiahao Wu,Tianzheng Ding,Wanting Qiu,Jingjing Fang,Zanfang Jin
Copper (Cu) contamination in soils, especially sludge-made nutrient soils, poses environmental and agricultural risks due to its high bioavailability and toxicity. Current stabilization methods often lack efficiency and selectivity. Here, we developed a cysteine-modified chitosan composite (Cys-CS) as an eco-friendly stabilizer for targeted Cu remediation. In the single-metal system, Cys-CS's adsorption capacity for Cu gradually increases as solution pH rises from 3.5 to 6.5, reaching the maximum at pH near 6.5, with a Langmuir maximum adsorption capacity of 97.8 mg/g (1.54 mmol/g). In mixed-metal systems, Cu adsorption (0.687–1.718 mmol/g) greatly exceeded that for Pb, Zn, Cd, and Ni (0.003–0.120 mmol/g), giving K Cu / K other ratios 11.09–118 and demonstrating strong selectivity. Soil incubation with only 0.5wt‰ Cys-CS transformed Cu from bioavailable fractions (BCR1/BCR2) into stable forms (BCR3/BCR4), while preserving soil pH, soil organic matter, cation exchange capacity, and microbial community structure, and minimally affecting non-target metals. Field-relevant trials in Cu-polluted sludge-made nutrient soils confirmed its high stabilization efficiency and negligible ecological disturbance. FTIR spectroscopy reveals that functional groups of L-cysteine, including SH, NH , and CONH 2 , form coordination bonds with Cu 2+ . Density functional theory (DFT) calculations further confirm Cu-selective binding: the interaction energy of Cys-CS with Cu 2+ is significantly lower than with coexisting metals (Pb, Zn, Cd, Ni), and CONH 2 coordination with Cu exhibits a lower band gap energy (Egap) than other Cys-CS functional groups, indicating higher binding affinity. This work demonstrates that functional group regulation of biopolymer-based adsorbents enables precise, low-dose, and environmentally safe remediation of Cu-contaminated soils, offering a practical pathway toward selective in situ stabilization in complex multi-metal environments.
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PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·
IF 2.8
Lin Lin,Xu Liu,Ce Shi,Xiaochen Chen,Tariq Aziz,Fahad Al-Asmari,Amal A. Mohamed,Haiying Cui
The objective of this research was to develop and analyse a chitosan- Tremella fuciformis polysaccharide (CS-TFP) edible film using chitosan (CS) and Tremella fuciformis polysaccharide (TFP) as the primary raw materials. The findings revealed significant improvements in the thickness, solubility, opacity, mechanical properties and barrier properties (water vapour and oxygen permeability) of the chitosan film upon the addition of TFP, but water content and hydrophobicity were reduced. Moreover, TFP addition markedly enhanced the antibacterial and antioxidant activities of the chitosan films. Structural analysis of the CS films and CS-TFP edible films indicated that the formation of hydrogen bonds and high compatibility between CS and TFP. Specifically, TFP increased crystallinity, reduced roughness and improved the compactness of the edible film. This increase in compactness was directly linked to enhanced physical properties, whereas increased crystallinity contributed to better thermal stability. Furthermore, the application of CS-TFP-3 edible films in chicken preservation proved that they could significantly delay the deterioration of chicken. Findings in this study suggested that CS-TFP edible film as a bioactive packaging material showed great potential to preserve chicken meat and extend the shelf life.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Longfei Wang,Jingyi Hu,Meng Zhou,Huaen Yuan,Huan Lu,Jingyi Chen,Mengyu Zhao,Menglan Xiao,Mingqin Zhao,Miao Lai
Heterocyclic fragrance compounds possess unique aromas that can enhance flavor of products, but their applications are limited due to high volatility, short storage durations, and thermal instability. This paper used porphyrin grafted carboxymethyl chitosan (CMCS) as wall material and selected four heterocyclic fragrance compounds as core materials to develop an effective controlled release system of photothermal-responsive microcapsules. The optimal preparation process for microcapsules was determined through response surface methodology (RSM). The Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and Flourier transform Infrared Spectroscopy (FTIR) results confirmed the successful preparation of microcapsules. Photo-release performance and thermo-gravimetry (TG) demonstrated excellent photothermal-responsive behavior. The results of MD indicated that the system of microcapsule exhibited enhanced stability and superior convergence, with van der Waals forces being the primary interaction between hosts and guests. Notably, the 3-acetylpyridine/5-( p -aminophenyl)-10,15,20-triphenylporphyrin-carboxymethyl chitosan (ATPD/AC) displayed strongest intermolecular force among the other microcapsules, which performed the highest complexation strength with guest and the highest stability. This study provided a theoretical foundation for constructing stable controlled release systems for heterocyclic fragrance microcapsules.
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ACS Omega
·
2025
·
IF 4.3
Huibin Yu,Hongqin Ke,Tu Chen,Jia Huang,Lei Zhou,Shenqi Wang
This study aimed to develop a novel adsorbent, Zn2+-loaded hexanediamine–chitosan (HDA-CS) beads, for the selective removal of human testosterone from plasma. The preparation involved synthesizing chitosan beads, modifying them with hexanediamine to enhance adsorption capacity and subsequently loading them with zinc ions. The effects of the HDA spacer, Zn2+ concentration, and adsorption time on the adsorption percentage for testosterone were systematically investigated through a series of adsorption experiments. Results indicated that the Zn2+-HDA-CS beads achieved a maximum adsorption capacity of approximately 35% within 90 min. The analysis of plasma concentrations of Zn2+, albumin, and sex hormone-binding globulin (SHBG) indicated that the adsorption process involved a complex interaction between testosterone-bound SHBG and albumin for Zn2+ binding sites on the beads. Additionally, the adsorbent demonstrated good storage stability and selectivity for testosterone, with minimal impact on total protein content in plasma. These findings highlight the potential of Zn2+-HDA-CS beads as an effective therapeutic option for managing testosterone levels in clinical settings, particularly in patients undergoing androgen deprivation therapy for prostate cancer.
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DESALINATION
·
2025
·
IF 9.8
Shengxing Yang,Chunpei Yan,Bin Huang,Tianxiang Jin,Dingge Guo,Minnan Xiao,Yong Qian
Nuclear energy is an essential part of the global energy mix, and its sustainable development relies on efficient and selective uranium extraction technologies. This study presents a 2-phosphonate butane 1, 2, 4-tricarboxylic acid (PBTCA)-modified self-crosslinked chitosan gel as a novel adsorbent for uranium extraction from wastewater. At an optimized voltage of −0.6 V, the material exhibited an impressive adsorption capacity of 631.94 mg·g −1 , along with excellent electrochemical performance and cycling stability. This innovative material, characterized by low energy consumption, high adsorption efficiency, and excellent regeneration capability, provides a promising solution for uranium recovery. This work highlights the potential of targeted chemical modifications in enhancing polymer-based electrosorption technologies.
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CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Yangmei Ren,Lanmei Huang,Yuelong Wang,Lan Mei,Rangrang Fan,Min He,Chao Wang,Aiping Tong,Haifeng Chen,Gang Guo
Skin damage, especially the extensive full-thickness wound, is seriously affecting people’s daily life and health. Meanwhile, wound healing is always challenged by bacterial infection. In this study, for the purpose of developing a disinfectant wound dressing, we designed a novel multi-functional nanofiber mats via electrospinning combining chitosan derivations and stereocomplex crystallite (SC). The SC membrane of poly (lactic acid)/chitosan derivatives were prepared via warming at 80 °C for 1 h. The thermal and mechanical properties of the heated mats were strengthened owing to the formation of SC, which restricted the lactide chains mobility. I n vivo wound healing test revealed that the SC mats have better wound repair ability than the control group with a wound healing rate of 100 % within 15 days. In a word, the biomass-based mats with enhanced thermal and mechanical properties, antibacterial effect and antioxidant activity, providing a potential multi-functional platform for designing of disinfectant wound dressings.
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MOLECULES
·
2022
·Vol. 27
·期 24
·8832
·
IF 4.927
Shengyou Ge,Xiaoyi Zhu,Chuanlong Zhang,Dongchen Jia,Wei Shang,Chao Ding,Jianping Yang,Yuanyong Feng
The strategy of incorporating bioactive inorganic nanomaterials without side effects as osteoinductive supplements is promising for bone regeneration. In this work, a novel biomass nanofibrous scaffold synthesized by electrospinning silica (SiO2) nanoparticles into polycaprolactone/chitosan (PCL/CS) nanofibers was reported for bone tissue engineering. The nanosilica-anchored PCL/CS nanofibrous bioscaffold (PCL/CS/SiO2) exhibited an interlinked continuous fibers framework with SiO2nanoparticles embedded in the fibers. Compact bone-derived cells (CBDCs), the stem cells derived from the bone cortex of the mouse, were seeded to the nanofibrous bioscaffolds. Scanning electron microscopy and cell counting were used to observe the cell adhesion. The Counting Kit-8 (CCK-8) assay was used. Alkaline phosphatase (ALP), Alizarin red staining, real-time Polymerase Chain Reaction and Western blot tests were performed to confirm the osteogenesis of the CBDCs on the bioscaffolds. The research results demonstrated that the mechanical property of the PCL together with the antibacterial and hydrophilic properties of the CS are conducive to promoting cell adhesion, growth, migration, proliferation and differentiation. SiO2nanoparticles, serving as bone induction factors, effectively promote the osteoblast differentiation and bone regeneration. This novel SiO2-anchored nanofibrous bioscaffold with superior bone induction activity provides a better way for bone tissue regeneration.Keywords:silica;polycaprolactone;chitosan;bioscaffold;osteogenesis;bone tissue engineering
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JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2018
·
IF 2.015
Liu Yang,Xiaofeng Liu,Nandi Zhou,Yaping Tian
Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine. Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body . Subsequently, acid urease was refolded gradually using dilution-ultrafiltration and specific activity of 12.3 U mg −1 was obtained. The acid urease thus obtained was immobilized on graphene oxide/chitosan beads. The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde. Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t 1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C. A significant change in K m occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state. The values of V max for immobilized EC and urea were 1.08 μmol min −1 and 1.16 μmol min −1 , respectively, whereas they were 0.782 μmol min −1 and 0.931 μmol min −1 , respectively, for soluble EC and urea. Furthermore, 90% of the original activity of graphene oxide/chitosan–urease beads were retained after the beads were used 10 times, indicating excellent reusability.
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