BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
·
2025
·
IF 4.2
Huilin Zhao,Shuzhen Liu,Jiangfan Shan,Juan Zhang,Qianwen Wu,Jianhui Zhang,Zekun Sun,Xinying Cao,Shu Chen,Mingzhu Feng,Yinuo Zhang,Xiang Cheng,Boqing Li,Xiaofei Ji
CagA, one of the key virulence factors of Helicobacter pylori , plays a significant role in H. pylori -associated gastric cancer by actively participating in neoplastic transformation. Among CagA variants, East Asian type CagA (CagA E ) bearing the EPIYA-D motif exhibits a higher risk than the Western-type (CagA W ) with EPIYA-C motifs. In this study, we investigated the interactions of CagA E and CagA W and host intracellular Grb2 and found a pronouncedly greater recruitment of Grb2 by CagA E compared to CagA W . Our findings revealed the phosphorylated tyrosine in the EPIYA motif is very important for the binding of CagA to Grb2. Phe at Y + 5 position in EPIYA-D, which interacts with Trp 121 in SH2 domain of Grb2, established the higher affinity than the interaction of EPIYA-C and Grb2-SH2. The substitute of Phe to Asp/Ala in CagA E EPIYA-D reduced the recruitment of Grb2 by CagA E , and neutralized the stronger induced malignant characteristics of the recipient cells. These findings provide additional insights into the distinct regulatory mechanisms employed by CagA E and CagA W , contributing to a better understanding of the higher oncogenic risk associated with H. pylori CagA E .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2025
·
IF 3.7
Qian Zhu,Kangning Wei,Kaihua Pan,Gang Hu,Weihao Zhu,Yanni Huang,Changchang Wang,Qian Li,Mingliang Zhang,Jiguo Qiu,Qing Hong
Isoprocarb (IPC), a widely used carbamate insecticide, causes environmental contamination and poses risks to humans and ecosystems. Rhodococcus sp. D-6, capable of utilizing IPC as the sole growth substrate, was isolated by our lab. Strain D-6 initiates IPC degradation through ester bond hydrolysis by the hydrolase IpcH, yielding 2-isopropylphenol (IPP). In this study, the catabolic pathway of IPP in strain D-6 was elucidated, and the ipp gene cluster responsible for its degradation was predicted by transcriptomic analysis. Key genes, including a novel two-component IPP monooxygenase gene ippA1A2 and a 2-isopropylhydroquinone dioxygenase gene ippB, are responsible for the successive conversion of IPP, resulting in the ring cleavage of IPP. IppA1, a group D flavin-dependent monooxygenase, along with its reductase component IppA2, hydroxylates IPP to 2-isopropylhydroquinone using reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) as cofactors. The optimal molar ratio of IppA1 to IppA2 was 3:1. The apparent Km and kcat values of IppA1 for IPP were 60.52 ± 1.27 µM and 70.07 ± 0.85 s−1, respectively. IppB, an extradiol dioxygenase, catalyzes 2-isopropylhydroquinone ring cleavage to generate 2-isopropyl-4-hydroxymuconic semialdehyde. The apparent Km and kcat values of IppB for 2-isopropylhydroquinone were 29.07 ± 1.54 μM and 147.65 ± 1.92 s−1, respectively. This study provides critical insights into the molecular mechanisms of IPP degradation, contributing to a comprehensive understanding of IPC degradation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Zhengyang Miao,Guangzheng Zhu,Lei Yang,Peng Ding,Fang Jiang
Polymer-based thermally conductive composites have garnered significant attention in recent years due to their critical applications in areas of national strategic importance. Nevertheless, the inherently limited thermal conductivity and mechanical properties of polymers pose challenges to their practical application. Recent research indicates that employing exogenous methods and facilitating molecular-level interactions between fillers and cross-linkers are effective strategies to overcome these limitations. In this study, with the aid of the ionic effect during the sol-gel phase transition of aqueous sodium alginate molecules in the presence of divalent cations and biaxial stretching, combined with the hydrogen bonding between boron nitride nanosheets (BNNS) with excellent thermal conductivity and sodium alginate (SA) molecules, the BNNS-calcium alginate (BNNS/Ca-SA/D) films were constructed by the strategy of gel reconstruction, rehydration, evaporation-assisted self-assembly and biaxial stretching. By designing anisotropic structures with closely aligned interconnected BNNS (Herman orientation parameter as high as 0.865), the BNNS/Ca-SA/D films with a BNNS loading of 40 wt% were characterized by high in-plane thermal conductivity (45.50 W•m −1 •K −1 ) and good mechanical properties. Combined with finite element analysis to simulate the heat transfer process, the “thermo-mechanical” coupled multilevel oriented structure composite films were constructed due to the densification arrangement and the increase of interfacial adhesion during multiple cross-linking processes, and the orientation arrangement of BNNS during the tensile-induced process. The potential of the BNNS/Ca-SA/D films developed in this study for thermal management applications has been demonstrated by the practical application of thermal management devices and the human body. The polymer-based thermally conductive films developed in this paper offer promising applications for thermal management systems in modern power electronics.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ziqiang Wu,Jinhu Wang,Kaiwen Wang,Zhuoqi Gong,Yihao Hu,Haoran Zhou,Tengyu Chen,Kaiqi Zhao,Lu Xia,Yingtang Zhou,Yao Zhang
Solid electrolytes offer a transformative solution to the safety challenges that have long hindered conventional lithium-ion batteries (LIBs), which rely on organic liquid electrolytes. Among them, hydride-based materials such as LiBH 4 stand out due to their exceptional electrochemical stability and strong compatibility with anode materials. However, their inherently poor ionic conductivity at room temperature (RT, ~10 −8 S cm −1 ) remains a critical bottleneck, primarily attributed to the rigid crystalline framework of hydride structures, which impedes lithium-ion diffusion. In this study, we overcome this limitation by introducing defective hexagonal boron nitride (d-BN) and iodine solutes into a LiBH 4 matrix.(i.e. Li 4 (BH 4 ) 3 I/2(d-BN)) This strategic integration induces structural deformation in the BH 4 tetrahedra, creating optimized pathways for enhanced lithium-ion transport. As a result, the composite achieves an impressive ionic conductivity of 5.9 × 10 −4 S cm −1 at 40 °C and 4.0 × 10 −4 S cm −1 at 25 °C, approaching the conductivity requirement for the commercialization of solid-state electrolyte batteries (10 −3 S cm −1 ), positioning this material among the highest-performing solid hydride electrolytes to date. Mechanistic insights from density functional theory (DFT) and molecular dynamics (MD) simulations revealed that iodine and BN incorporation weakens Li B interactions, extends Li H bond lengths, and reduces the energy barrier for Li-ions migration. In addition, this gain in conductivity is accompanied by a strong cycling performance (The capacity retention rate of the optimized TiS 2 |Li 4 (BH 4 ) 3 I/2d-BN|Li battery exceeds 86 % after 30 charge-discharge cycles at 0.1C). These findings highlight the promise of Li 4 (BH 4 ) 3 I/d-BN composites as efficient solid-state electrolytes for next-generation energy storage applications.
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Rong Cao,Fei Yu,Jing Chen,Jian Yin,Jun Liu,Xuwei Long
This study developed a novel strategy to prepare deacetylated mannosylerythritol lipids (MEL-D), which can disrupt cell membranes, from fermented MELs to address the challenge of inhibiting drug-resistant bacteria. Approximately 45 % of MEL-D with a purity exceeding 98 % can be obtained through acid hydrolysis of crude MELs and an integrated separation and purification process. The resulting MEL-D exhibited superior antibacterial activities compared to crude MELs against Bacillus cereus and Bacillus subtilis, with minimum inhibitory concentrations (MIC) ranging from 32 to 64 mg/L. This effective antibacterial action arises from its ability to disrupt cell membranes and biofilms. However, MEL-D demonstrated weaker antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), with an MIC as high as 512 mg/L. When combined with antibiotics, MEL-D significantly enhanced antibacterial activity and reduced the required dosage, exhibiting strong synergy with conventional antibiotics, as evidenced by FICI values in the range of 0.25–0.5. Moreover, this strategy demonstrates a favorable synergy profile when combined with antibiotics, which significantly mitigates the cytotoxicity associated with MEL-D monotherapy.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 15
·3604
·
IF 4.2
Qiang Fu,Mingqiang Chen,Weixin Pang,Zengqi Liu,Zhen Xu,Xin Lei
The thermodynamic effect of octyl-β-D-glucopyranoside (OGP) on the formation of methane-1,3-dimethylcyclohexane (DMCH) hydrate was studied in this work. The thermodynamic equilibrium hydrate formation pressures between 275.15 K and 283.15 K were measured by the isothermal pressure search method. Different OGP aqueous solutions (0, 0.1, and 1 wt%) were used in this work. The experimental results show that OGP had no obvious thermodynamic inhibition on methane-DMCH hydrate formation when its concentration was low (0.1 wt%), whereas it had an inhibition on methane-DMCH hydrate formation when its concentration was high (1 wt%). The phase equilibrium hydrate formation pressure of the methane-DMCH-OGP system is about 0.1 MPa higher than that of the methane-DMCH system. The dissociation enthalpies of methane hydrate in different solutions remained uniform, which indicates that OGP was not involved in methane-DMCH hydrate formation. This phenomenon is explained from the perspective of the molecular structure of OGP. As a renewable and biological nonionic surfactant, the concentration of OGP in the liquid phase is low, so OGP can be added to the methane-DMCH system without significant thermodynamic inhibition.
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Huanlin Li,Wei Shi,Yusong Zhang
Terahertz time-domain spectroscopy (THz-TDS) technology offers unique advantages for biomedical applications and non-destructive testing. Unlike X-rays, THz technology does not damage the materials being tested, especially active substances. However, traditional THz-TDS techniques still struggle to distinguish between chiral substances. This study presents a terahertz polarization time-domain spectroscopy system (THz-PTDS) that utilizes a 2 × 2 GaAs photoconductive array to detect the polarization of terahertz waves. This system enables the non-destructive and rapid identification of chiral forms of malic acid and its diethyl ester enantiomers. The results indicated that the polarization parameter curves for both solid and solution forms of malic acid showed significant chiral differences at frequency of 0.6 THz. Moreover, the polarization parameter curve of a malic acid diethyl ester-ethanol solution displayed significant chiral differences at 0.93 THz, while the pure malic acid diethyl ester showed significant chiral differences at 1.013 THz. Furthermore, the rotation direction of the polarization ellipse at the corresponding THz frequencies can be used to distinguish between enantiomers. This method provides an innovative approach for the rapid identification of chiral enantiomers in food and pharmaceuticals, with significant application prospects and potential benefits.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2016
·Vol. 33
·期 7
·388-395
·
IF 4.367
Wenbin Wang,Liqiang Liu,Liguang Xu,Hua Kuang,Jianping Zhu,Chuanlai Xu
Staphylococcal enterotoxins (SEs) in food samples can cause severe gastroenteritis even at low doses. Detection of SEs with a single assay would not only improve reliability and portability, but would also save time. In this paper, for the first time, a multiplexed gold-nanoparticle-based immunochromatographic (ICT) strip that allows the simultaneous detection of the five main SEs has been reported: SEA, SEB, SEC, SED, and SEE. Based on the sandwich immunoassay of each SE, a multiplexed ICT strip with five test lines is developed. Sensitive and specific paired monoclonal antibodies facilitate the detection, allowing for high sensitivity and good specificity. The portable biosensor platform can simultaneously detect SEA, SEB, SEC, SED, and SEE at concentrations as low as 2.5, 2.5, 2.5, 1, and 5 ng mL −1 , respectively. The entire test can be performed within 15 min and requires no sophisticated instruments except a portable strip reader. To evaluate the multiplexed strip, naturally SEA and SEC contaminated milk samples and pure milk samples spiked with the five SEs are analyzed.
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Yu Zhang,Bo Xia,Yanyan Li,Xianfu Lin,Qi Wu
The synthesis of optically pure polymers is one of the most challenging tasks in polymer chemistry. Herein, Novozym 435 (Lipase B from Candida antarctica, immobilized on Lewatit VP OC 1600)-catalyzed polycondensation between d-/l-aspartic acid (Asp) diester and diols for the preparation of helical chiral polyesters was reported. Compared with d-Asp diesters, the fast-reacting l-Asp diesters easily reacted with diols to provide a series of chiral polyesters containing N-substitutional l-Asp repeating units. Besides amino acid configuration, N-substituent side chains and the chain length of diols were also investigated and optimized. It was found that bulky acyl N-substitutional groups like N-Boc and N-Cbz were more favorable for this polymerization than small ones probably due to competitively binding of these small acyl groups into the active site of Novozym 435. The highest molecular weight can reach up to 39.5 × 103 g/mol (Mw,Đ = 1.64). Moreover, the slow-reacting d-Asp diesters were also successfully polymerized by modifying the substrate structure to create a “nonchiral” condensation environment artificially. These enantiocomplementary chiral polyesters are thermally stable and have specific helical structures, which was confirmed by circular dichroism (CD) spectra, scanning electron microscope (SEM), and molecular calculation.
✓ 已验证
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Limin Ren,Yuxuan Xiao,Rameswar Bhattacharjee,Jingjing Wu,Pengyi Tang,Stavros Caratzoulas,Changgong Meng,Qiang Guo,Michael Tsapatsis
Graphical Bifunctional Ti-B-Beta zeolite is demonstrated to be an efficient catalyst for sorbose production via glucose isomerization. Ethanol directs the conformation of glucose, facilitating its selective isomerization to sorbose and enables this isomerization to surpass the thermodynamic equilibrium limit by engaging in reactions with the resultant sorbose. D-Glucose-to-L-sorbose isomerization on Lewis acidic zeolite is a highly attractive avenue for sorbose production. But the L-sorbose yield is limited by the competing D-glucose-to-D-fructose isomerization and reaction equilibrium. In this work, it is suggested that ethanol directs the glucose conformation for selective D-glucose-to-L-sorbose isomerization. It also reacts with the produced L-sorbose to form ethyl-sorboside, which allows the D-glucose-to-L-sorbose isomerization to proceed beyond the thermodynamic equilibrium limit. It is shown that a bifunctional zeolite Beta containing framework titanium (Ti) and boron (B) is a selective catalyst for this tandem reaction: Lewis acidic framework Ti catalyzes the D-glucose-to-L-sorbose isomerization via an intramolecular 5,1-hydride shift process as confirmed by isotopic tracing experiments followed by 13 C-NMR, while weak Brønsted acid framework B selectively promotes the sorbose ketalization with ethanol. A remarkably high yield of L-sorbose with a high fraction of sugar (>95 %: 27 % unreacted glucose, 11.4 % fructose, 57 % sorbose) was obtained after the mixture produced in ethanol was hydrolyzed.
✓ 已验证
DOI
高影响因子
Energy Technology
·
2022
·Vol. 10
·期 7
·2200262
·
IF 4.149
Tian Zhang,Xichuan Yang,Li Zhang,Zijian Deng,Kaiyuan Yang,Haiyang Cui,Qingning Hou,Wenxi Ji
The lattice defects in perovskite solar cells comprising polycrystalline films affect the cell's humidity stability and photovoltaic performance. Herein, a donor–pi–acceptor organic dye molecule ( ZT001 ) containing a carboxyl group on the acceptor moiety and long alkyl chains on the donor moiety is synthesized and used as an effective passivator in perovskite solar cells. It is revealed in the results that the carboxyl group of ZT001 successfully passivates the film defects by forming coordination with uncoordinated Pb 2+ , and the humidity stability of the device is improved and maintained above 80% of the original photoelectric conversion efficiency (PCE). Therefore, ZT001 -passivated perovskite devices exhibit a superior PCE of 20.6% compared with the PCE of unpassivated devices (19.5%).
✓ 已验证
DOI
高影响因子
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
·
2021
·Vol. 25
·期 2
·131-137
·
IF 2.016
Jing Cheng,Chaoyang Ren,Renli Cheng,Yunning Li,Ping Liu,Wei Wang,Li Liu
Aging is the process spontaneously occurred in living organisms. Cardiac fibrosis is a pathophysiological process of cardiac aging. Mangiferin is a well-known C-glucoside xanthone in mango leaves with lots of beneficial properties. In this study, rat model of cardiac fibrosis was induced by injected with 150 mg/kg/d D-galactose for 8 weeks. The age-related cardiac decline was estimated by detecting the relative weight of heart, the serum levels of cardiac injury indicators and the expression of hypertrophic biomakers. Cardiac oxidative stress and local inflammation were measured by detecting the levels of malondialdehyde, enzymatic antioxidant status and proinflammatory cytokines. Cardiac fibrosis was evaluated by observing collagen deposition via masson and sirius red staining, as well as by examining the expression of extracellular matrix proteins via Western blot analysis. The cardiac activity of profibrotic TGF-β1/p38/MK2 signaling pathway was assessed by measuring the expression of TGF-β1 and the phosphorylation levels of p38 and MK2. It was observed that mangiferin ameliorated D-galactose-induced cardiac aging, attenuated cardiac oxidative stress, inflammation and fibrosis, as well as inhibited the activation of TGF-β1/p38/MK2 signaling pathway. These results showed that mangiferin could ameliorate cardiac fibrosis in D-galactose-induced aging rats possibly via inhibiting TGF-β/p38/MK2 signaling pathway.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）