Frontiers in Nutrition
·
2023
·
IF 5
Jinlin Cai,Zijian Liang,Jian Li,Muhammad Faisal Manzoor,Hongsheng Liu,Zhong Han,Xinan Zeng
Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides. Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree – 1, 2, 3, 4, and 5). Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays. Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC. The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated. The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect. Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides. However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics. Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC 50 : 1.06 mg/mL) and ABTS (IC 50 : 1.22 mg/mL) radicals. The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight. Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA. The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart. Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability. Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction. This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Jian-Jun Li,Dan Qiao,Jing Zhao,Guo-Jun Weng,Jian Zhu,Jun-Wu Zhao
The applications of metallic nanoclusters and nanoparticles in biological sensing have attracted special attention owing to their optical interaction based on fluorescence and surface plasmon resonance (SPR). In this work, we designed a fluorescent nanoprobe for the determination of L -cysteine ( L -Cys) based on fluorescence resonance energy transfer (FRET) from gold‑silver bimetallic nanoclusters (Au-Ag NCs) to gold nanorods (AuNRs). Firstly, the negatively charged Au-Ag NCs protected by bovine serum albumin (BSA) are directly adsorbed on the surface of the positively charged AuNRs through electrostatic interaction , and the FRET effect leads to distinct fluorescence quenching of Au-Ag NCs at 615 nm. The SPR wavelength of AuNRs is dependent on the aspect ratio, so the SPR of AuNRs could be tuned to have a better spectral overlap with fluorescence of Au-Ag NCs, which enhances the fluorescence quenching effect. Because the SH group of L -Cys has an affinity with gold, the addition of L -Cys can result in the release of Au-Ag NCs from the surface of AuNRs via forming Au S bonds. Thus, the introduction of L -Cys could effectively restore the fluorescence emission of the AuNRs/Au-Ag NCs system because of the restraint of FRET effect. Under the optimized conditions, the fluorescence recovery of AuNRs/Au-Ag NCs probe exhibits a linear response to L -Cys concentration ranging from 5 to 100 μM, and the corresponding theoretical detection limit (LOD) is 1.73 μM. Meanwhile, this method displays excellent sensitivity and selectivity for L -Cys over other amino acids , and it has been successfully applied to detect L -Cys in real samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Chaoyang Li,Hua Zhang,Xianfeng Gong,Qiang Li,Xueling Zhao
The chemical stability, good biocompatibility and high drug loading capacity of zinc oxide nanoparticles (ZnO NPs) and their biomedical potentials make them a promising candidate for drug delivery. The aim of this study was to develop and assess a simple procedure for surface functionalization of ZnO NPs by N-acetyl- l -cysteine (NAC) for anticancer camptothecin (CPT) delivery. NAC capped ZnO NPs were successfully made using ZnCl 2 and NaOH in the presence of NAC. CPT was covalently conjugated to the surface of as-synthesized ZnO-NAC NPs. To characterize the synthesized conjugate product (ZnO-NAC-CPT NPs), X-ray diffraction, Fourier Transform Infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and dynamic light scattering method were used. Our results indicated that the ZnO-NAC-CPT NPs exhibit near-spherical morphology and uniform dispersion with an average diameter of ∼70 nm. The hemolysis assay showed that ZnO-NAC-CPT NPs has almost no hemolytic activity. In addition, MTT cytotoxicity assessment on A549 lung cancer cells revealed a drop of IC 50 values from 1.17 μg/mL (free CPT) to 0.66 μg/mL (ZnO-NAC-CPT NPs). This result showed an augmented cancer-inhibitory effect of nanoconjugate complex. In conclusion, the novel ZnO-NAC-CPT NPs could be considered for new therapeutic endeavors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2018
·
IF 3.641
Sidi Zhu,Mingzhu Xia,Yuting Chu,Muhammad Asim Khan,Wu Lei,Fengyun Wang,Tahir Muhmood,Along Wang
Herein, we reported the adsorption and desorption of Pb(II) on l -lysine modified montmorillonite (L-Mt) with molecular dynamics simulation. l -lysine (Lys) modified montmorillonite (L-Mt) was prepared, characterized by various analytical techniques, and the simulation studies were performed to evaluate the interlayer structure and interaction properties of l -lysine and L-Mt. The results of X-ray powder diffraction (XRD) and Fourier transform infrared (FTIR) spectrum suggested that Lys + was intercalated into the interlayer of Na-montmorillonite (Na Mt) successfully. The batch adsorption experiments were implemented to study the adsorption capacity of Pb(II) on L-Mt in water. The maximum adsorption capacity of Pb(II) on the L-Mt (up to 43.3 mmol/100 g) were considerably higher than that on Na Mt (up to 15.3 mmol/100 g), at room temperature. The adsorption isotherms of both, L-Mt and Na Mt can fit the Langmuir isotherm . The adsorption experiments showed that Pb(II) removal efficiency of L-Mt is better than Na Mt in acidic solution. The simulation results revealed the micro-mechanism of Lys + inserted into the interlayer gallery of Na Mt. It can be deduced that lysine interacted with montmorillonite surface mainly through the protonated side-chain amino group rather than through the protonated main-chain amino group or deprotonated carboxyl group . These consequences are expected to elucidate the adsorption mechanisms occurring at the water/clay minerals interface at atom level, providing significant insight into the adsorption and retention of Pb(II) on L-Mt composite, and lead to relevant engineering applications while treating heavy metals of wastewater.
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Phongphat Prawang,Yongqiang Zhang,Ying Zhang,Hui Wang
Poly(ethylene glycol) (PEG), a kind of “green” medium, was used to extract artemisinin from the Chinese herb Artemisia annua L. to replace the traditional volatile and explosive solvents like petroleum ether, diethyl ether, etc. PEGs, with different molecular weights, show excellent ability for artemisinin extraction, and the extraction efficiency decreased with the increase of molecular weight. It was also demonstrated that ultrasonic irradiation could further enhance extraction efficiency compared with conventional water bath heating. Results indicate that the extraction amount of artemisinin from one gram of the leaves could reach 15.8 mg/g under the optimal conditions with ultrasonic power of 100 W, the temperature of 50 °C, time of 1 h in PEG-200, which were optimized by response surface method. Compared with Soxhlet extraction using petroleum ether (PE), the method developed here shows higher extraction efficiency (15.8 mg/g for PEG vs. 14.9 mg/g for PE process) and shorter extraction time (1 h vs. ca. 12 h). Additionally, artemisinin in the PEG solution can be recovered using resin adsorber. The results show that the recovery of artemisinin could reach almost 100% by D101 or H103 porous resin, and then the drug could be desorbed from the resin by ethanol or ethyl acetate. Moreover, the kinetic and mechanism studies of the extraction process revealed that ultrasonic irradiation could enhance mass transfer and the hydrogen bonding interactions between artemisinin and PEG further facilitate the separation of artemisinin from the leaves. This work provides an alternative process for efficient artemisinin extraction by using an environmentally benign medium with outfield intensification, which could be potentially extended to the production of other natural drugs.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Juan Qiao,Liping Zhao,Lili Liu,Li Qi
Inspired by the porous and fibrous structure of commercially available bamboo, herein we created an l -glutaminase enzyme reactor based on bamboo sticks. The enzyme was immobilized onto the bamboo sticks through a glutaraldehyde modification to achieve covalent bonding. The enzymatic hydrolysis efficiency of the prepared l -glutaminase@bamboo sticks based porous enzyme reactor was evaluated by chiral ligand exchange capillary electrochromatography using l -glutamine as the substrate. l -glutaminase@bamboo exhibited improved enzymatic hydrolysis performances, including high hydrolysis efficiency (maximum rate V max : two fold higher than the free enzyme), prolonged stability (14 days) and good reusability. l -Glutaminase@bamboo sticks also expanded application capability in pharmaceutical industry in enzyme inhibitor screening. These excellent properties could be attributed to the micropores of bamboo sticks, which led to the fast enzymatic kinetics. The results suggest that the pores of bamboo sticks played an important role in the proposed enzyme reactor during the hydrolysis of l -glutamine and l -glutaminase inhibitor screening.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 8
·4868-4876
·
IF 3.241
Xiaoyong Chen,Xiong Li,Xiaobo Zhang,Lijun You,Peter Chi-Keung Cheung,Riming Huang,Jianbo Xiao
In this study, a polysaccharide was extracted from Physalis pubescens L. (named PP). Its antihyperglycemic and antihyperlipidemic activities were evaluated in STZ-induced diabetic mice. Results showed that PP was determined to be composed of rhamnose (Rha), arabinose (Ara), fucose (Fuc), xylose (Xyl), mannose (Man), glucose (Glc) and galactose (Gal) with molar percentages of 4.65%, 17.34%, 1.43%, 6.24%, 5.52%, 45.5%, and 19.31%, respectively. The average molecular weight (Mw) was found to be 20.0 kDa. It had a strong α-glucosidase inhibitory activity in vitro. PP treatment could enhance the oral glucose tolerance, and increase the levels of SOD, GSH, CAT, vitamin C, vitamin E, HDL-c, C-peptide, GCK and hepatic glycogen in diabetic mice. Besides, PP treatment could also decrease the levels of MDA, TG, TC, LDL-c, BUN and G-6-Pase. The regulating effects were stronger in high dose PP treatment than those in the low and medium dose treatments. In short, PP played an important role in protecting STZ-induced diabetic mice, and the effect was closely related to its activities in antioxidation and regulating glucose and lipid metabolism.
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Qi-hao Yu,Fan Zhu,Juan-juan Su,Jian Han
The poly( l -lactide) grafted multi-walled carbon nanotubes (MWNT-g-PLLA) was firstly prepared, and a maximum grafting amount of 31 wt% was obtained by optimizing grafting reaction conditions. Then the PLLA/MWNT-g-PLLA composite fibers were fabricated by melt spinning, and showed significant enhancement of tensile strength and elongation at break, comparing with the unmodified PLLA/MWNTs or pure PLLA ones. Structure analysis of as-spun fibers revealed uniform dispersion of MWNT-g-PLLA in PLLA matrix on account of good interfacial compatibility, which could prevent premature interface damage and ensure the continuous deformation of fibers under stress. The crystal morphology of strain hardening before unbroken fibers were expressly observed, suggesting a possible stretching induced formation of nano-hybrid shish-calabash similar to structure in which the PLLA lamellae growing onto the MWNT-g-PLLA axis. This interface crystallization greatly improved the interfacial stress transferring between PLLA and MWNT-g-PLLA, thus resulted in notable promoted tensile strength and elongation at break.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Hafiz Muhammad Saleem Akhtar,Mohamed Abdin,Yahya Saud Hamed,Wei Wang,Guijie Chen,Dan Chen,Chunxu Chen,Wei Li,Shanza Mukhtar,Xiaoxiong Zeng
In the present study, polysaccharides (CHPS) were prepared from chickpea hulls by hot water extraction and ethanol precipitation, and the physicochemical, functional, thermal and structural characteristics of CHPS were investigated. The results revealed that CHPS was comprised of mannose , rhamnose, galacturonic acid, glucose, galactose , xylose and arabinose with molar percentages of 2.16, 2.96, 42.17, 9.87, 23.15, 6.29 and 13.38%, respectively. Functional analysis demonstrated that CHPS had good fat-binding, foaming and emulsifying properties. CHPS also exhibited good thermal stability. According to X-ray spectrum, CHPS was a semi-crystalline in nature which was also confirmed from scanning electron microscopic analysis. The solid-state spectrum of 13 C NMR reflected that CHPS were complex and heterogeneous polymers. Moreover, CHPS showed favorable inhibitory activity against α-amylase. These findings indicated that CHPS could be exploited as a hypoglycemic agent or multi-purpose additive in pharmaceuticals, cosmetics and food industries.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Mohammad Chand Ali,Jia Chen,Haijuan Zhang,Zhan Li,Liang Zhao,Hongdeng Qiu
The efficient extraction of natural products from biomass is deemed an essential area of interest using green technology in the pharmaceutical and biochemical industries. In recent years, deep eutectic solvents (DESs) have been paying broad interest as compatible, safe, and inexpensive solvents. In this study, we addressed, for the first time, to give a fruitful illustration to extract bioactive compounds from favourite Lycium barbarum L. fruits using DESs as green designer solvents, and displaying the tuneability of DESs to extract bioactive compounds from biomass efficiently. Among the studied DESs, the 1 : 2 M mixture of choline chloride and p -toluene sulfonic acid (DESs-6) with an ultra-sound assisted extraction (UAE) method was more efficient to get high extraction yields of flavonoids including myricetin (57.2 mg/g), morin (12.7 mg/g), and rutin (9.1 mg/g) in net DESs. The resulting extraction method in which 50 mg of sample powder was extracted by UAE for 1.5 h using DESs-6 was found to be a more efficient process than conventional extraction methods such as heating plus stirring. Operational conditions including the extractant to sample solid ratio, the effect of water content, the effect of temperature, recycling of DESs, and the effect of ultra-sound irradiation time were investigated. The proposed DESs based extraction procedure attained recovery for flavonoids were in the range 75.6%–96.9%. Therefore, p -toluenesulfonic acid-based DESs may have strong potential as promising materials for the green and efficient extraction of pharmaceutical compounds from other plants or fruits.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2018
·Vol. 316
·期 1
·71-80
·
IF 1.181
Chen Huai,Chen Quan Shui,Huang Bin,Wang Shi Wei,Wang Ling Yu
In this work, a brand new organic superficial active sorbent l -Cysteine hydrochloride modified bentonite (LCMB) has been developed and proposed for treatment of contaminated water containing uranium. Correlation between different factors such as pH, contact time, temperature, initial concentration of U(VI) and efficiency of LCMB for uranium absorption are discussed. At uranium content in water from 25 to 250 mg L −1 , the sorption capacity for LCMB reached 208.3 mg g −1 that is essentially higher in comparison with other sorbents and notably improved from 77 mg g −1 after modification.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Chenguang Yang,Wantong Song,Dawei Zhang,Haiyang Yu,Lei Yin,Na Shen,Mingxiao Deng,Zhaohui Tang,Jingkai Gu,Xuesi Chen
Gemcitabine is widely used for anticancer therapy. However, its short blood circulation time and poor stability greatly impair its application. To solve this problem, we prepared a poly ( l -glutamic acid)- g -methoxy poly (ethylene glycol)-gemcitabine conjugate ( l -Gem) with a 14.3 wt% drug-loading content. l -Gem showed concentration- and time-dependent cytotoxicity towards 4T1, LLC, MIA PaCa-2 and A2780 in vitro . Pharmacokinetic and biodistribution studies indicated that l -Gem had remarkably enhanced blood stability, prolonged blood circulation time and greatly improved selective tumor distribution compared with free gemcitabine. The area under the concentration–time curve from zero to infinity [AUC (0–∞) ] of l -Gem in plasma was 43-fold higher than that of free gemcitabine. The AUC (0–∞) of the inactive metabolite, 2′-deoxy-2′,2′-difluorouridine in the l -Gem group was ∼20% of that observed in the free gemcitabine group. The drug tumor accumulation ratio in the l -Gem group relative to the free gemcitabine group was 9.9 at 36 h, while the tumor AUC ratio was 15.8. Testing on Balb/C mice bearing the 4T1 tumor further demonstrated that l -Gem had significantly higher anticancer efficacy than free gemcitabine in vivo . These findings indicated that l -Gem has great potential for cancer treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）