RSC Advances
·
2014
·Vol. 4
·期 82
·43624-43629
·
IF 3.708
Huiping Liu,Cheng-an Tao,Zhihong Hu,Sida Zhang,Jianfang Wang,Yonggong Zhan
A new graphene-based electrochemical biosensor was constructed and applied for the simple, rapid and highly selective determination of glucose. The modified glassy carbon electrode (GCE) was prepared using dopamine (DA) as a reducing agent for graphene oxide (GO) and as a capping agent to stabilize and coat the resulting reduced graphene oxide (RGO) surface as it can polymerize to form polydopamine (PDA). PDA-RGO composites were dropped onto a glassy carbon electrode surface, and glucose oxidase (GOx) was immobilized on the surface of the composites by Michael addition. Finally, Pt nanoparticles (PtNPs) were electrodeposited on the modified electrode. Such modified electrodes (denoted as GCE/PDA-RGO/GOx/PtNPs) were characterized by UV-visible spectroscopy, atomic force microscopy, X-ray power diffractometry, Fourier-transform infrared spectroscopy, and scanning electron microscopy. Electrochemical behaviors were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The fabricated biosensor exhibited a high sensitivity of 33 μA mM−1 cm−2 over a wide linear range of 0.2 mM to 1 mM, good stability, excellent repeatability, and a detection limit of 0.10 mM.
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 3
·2535-2551
·
IF 2.993
Shen Hao,Song Jiayi,Yang Ye,Su Ping,Yang Yi
The exploration of new tactics for manufacturing artificial immobilized multi-enzyme systems based on enzyme cascades has recently attracted considerable interest because of the urgent need for multi-enzyme catalysts and the high cost of free enzymes. Because of the inevitable limitations of native enzymes such as instability and storage issues, the development of nanozyme–enzyme cascades is needed. A versatile strategy was developed for fabricating an efficient multi-catalyst system by immobilizing glucose oxidase (GO x ) on ferriferous oxide nanocomposites functionalized with nitrogen-doped graphene quantum dots (Fe 3 O 4 @N-GQDs) through DNA-directed immobilization. The Fe 3 O 4 @N-GQDs acted as a carrier for the natural enzyme and showed high peroxidase activity which enabled an enzyme cascade that included GO x to be set up. This multi-catalyst system showed great catalytic activity, reversibility and operational stability. The surfaces of GO x -targeted magnetic nanoparticles were regenerated by mild dehybridization of DNA. The Michaelis constant ( K m ) and maximum initial velocity ( V max ) of the multi-catalyst system were 1.069 mM and 11.2 × 10 −8 M s −1 , respectively, which are considerably better than the corresponding values for adsorbed and free bienzyme combinations. The increased bioactivity of the multi-catalyst system is ascribed to the satisfactory peroxidase-like activity of Fe 3 O 4 @N-GQDs, the enzyme–promoting effect of the QDs and enhancement by DNA-directed immobilization. Because of the diverse range of possible nanozyme–enzyme combinations and high efficiency of this approach, this work provides a novel pathway for the manufacturing of synthetic enzyme catalyst systems, which have great potential in the field of biotechnology.
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Chengyu Zhang,Shengping You,Jiaxing Zhang,Wei Qi,Rongxin Su,Zhimin He
In this study, we used a simple in-situ biomineralization method to immobilize Bacillus subtilis (B. subtilis) -derived laccase into the copper-Trimesic acid framework (Cu-BTC), and the synthesized [email protected] particles were used to degrade tetracycline and ampicillin. Compared with free laccase, the [email protected] showed 16.5-fold of activity recovery, higher thermo-tolerant performance, more excellent acid-proof ability and reusability. Without any mediators, [email protected] displayed high degradation efficiency (nearly 100%) for tetracycline and ampicillin in some actual water. The degradation mechanism and proposed degradation pathways of tetracycline and ampicillin were discussed technically. Besides, bacteriostatic assay and survival test of Escherichia coli (E. coli) and B. subtilis confirmed the loss of antibiotic activity for tetracycline and ampicillin, as well as the low ecotoxicity of the degradation products. Our research demonstrates that [email protected] has excellent performance in the effective removal of antibiotics and the detoxification of degradation products, which make it a promising candidate for environmental recovery.
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Yao Zhu,Fengxian Qiu,Jian Rong,Tao Zhang,Kaili Mao,Dongya Yang
Biocatalytic removal with laccase immobilized on diverse membranes offers an attractive option to search alternative to traditional wastewater treatment processes for the removal of high toxic azo dye. In this work, the modified poly(vinylidene fluoride) membrane (PVDF) with chemical stability and high mechanical strength was developed for laccase immobilization via covalent bonding. The key design for the synthesis of biocatalytic membrane is the construction of hybrid bio-inorganic structure on the surface of polydopamine (PDA)-coated PVDF ( [email protected] ). In this respect, the PDA layer was used as a secondary platform for the grafting of 3-triethoxysilylpropylamine (APTES) modified Fe 2 O 3 @SiO 2 cubes ( [email protected] ) via a solvothermal process, resulting in the formation of [email protected] @PVDF membrane. Subsequently, laccase was immobilized on the surface of [email protected] [email protected] via gluteraldehyde (GA) crosslinking ( [email protected] [email protected] ). The removal efficiency of congo red by [email protected] @PVDF reached 97.1 % under optimal reaction conditions (pH 7.0 and temperature 35 ℃), which was more efficient than free laccase. Moreover, the as-prepared [email protected] @PVDF not only exhibited an excellent stability after low temperature storage, but also showed an outstanding reusability. Therefore, we believe that this work opens up a potential strategy for removal of other water pollutants, and provide a simple and convenient way for large-scale applications of enzyme-catalysis.
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Zepeng Kang,Yuanming Wang,Chennan Yang,Bing Xu,Lei Wang,Zhiguang Zhu
Herein, a novel N and O co-doped three dimensional (3D) hybrid carbon aerogel (CA) was prepared by a two-step carbonization technology, i.e. preoxidation process (240 °C, air atmosphere) and carbonization process (600∼1000 °C, nitrogen atmosphere). It was found that the peroxidation process could improve the thermostability and heteroatoms content during the carbonization. The obtained CA samples inherited their original morphology of “string-bead” network and had a higher heteroatoms content, while the control samples did not. We focused on the application of this CA in either lactate oxidase-modified, super-capacitive and oxygen reductive electrodes. The fabricated lactate-powered integrated electronic skin could produce a maximum power density of ca. 1.3 mW cm −2 , which allowed powering a multiplexed metabolic electronic in situ . The sample of CF-PVA-PANI 800 showed the highest specific capacitance of 427.2 F g −1 at a discharge specific current of 0.5 A g −1 . The synergistic effect of the N functionalities endowed the sample of CF-PVA-PANI 900 with similar electrocatalytic activity to a 20 wt% commercial Pt/C catalyst for ORR. This fabrication strategy of carbon aerogel may provide a new alternative for preparing more heteroatom-doped 3D carbon materials in future.
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2014
·
IF 2.538
Yuhao Ma,Xinxiu Bian,Liu He,Mengtan Cai,Xiaoxiong Xie,Xianglin Luo
Developments of poly(caprolactone) in blood-contacting applications are often restricted due to its intrinsic hydrophobicity. One common way to improve its hemocompatibility is to attach hydrophilic polymers. Here we developed a non-destructive method to graft hydrophilic poly(acrylamide) (PAAm) onto poly(caprolactone) (PCL) surface. In this strategy, azido-ended PCL with low molecular weights was synthesized and blended with PCL to create a surface with “clickable” property. Alkyne-ended poly(acrylamide)s with controlled chain lengths were then synthesized by atom transfer radical polymerization (ATRP), and finally were immobilized onto PCL surface by “click” reaction. The occurrence of immobilization was verified qualitatively by water contact angle measurement and quantitatively by X-ray photoelectron spectroscopy (XPS). The PAAm grafted surface exhibited fouling resistant properties, as demonstrated by reduced bovine serum albumin (BSA) and fibrinogen (Fg) adhesion.
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Zhangtao Li,Lu Wang,Jizi Wu,Yan Xu,Fan Wang,Xianjin Tang,Jianming Xu,Yong Sik Ok,Jun Meng,Xingmei Liu
Zeolite-supported nanoscale zero-valent iron (Z-NZVI) has great potential for metal(loid) removal, but its encapsulation mechanisms and ecological risks in real soil systems are not completely clear. We conducted long-term incubation experiments to gain new insights into the interactions between metal(loid)s (Cd, Pb, As) and Z-NZVI in naturally contaminated farmland soils, as well as the alteration of indigenous bacterial communities during soil remediation . With the pH-adjusting and adsorption capacities, 30 g kg −1 Z-NZVI amendment significantly decreased the available metal(loid) concentrations by 10.2–96.8% and transformed them into strongly-bound fractions in acidic and alkaline soils after 180 d. An innovative magnetic separation of Z-NZVI from soils followed by XRD and XPS characterizations revealed that B-type ternary complexation, heterogeneous coprecipitation, and/or concurrent redox reactions of metal(loid)s, especially the formation of Cd 3 (AsO 4 ) 2 , PbFe 2 (AsO 4 ) 2 (OH) 2 , and As 0 , occurred only under specific soil conditions. Sequencing of 16S rDNA using Illumina MiSeq platform indicated that temporary shifts in iron-resistant/sensitive, pH-sensitive, denitrifying, and metal-resistant bacteria after Z-NZVI addition were ultimately eliminated because soil characteristics drove the re-establishment of indigenous bacterial community. Meanwhile, Z-NZVI recovered the basic activities of bacterial DNA replication and denitrification functions in soils. These results confirm that Z-NZVI is promising for the long-term remediation of metal(loid)s contaminated farmland soil without significant ecotoxicity.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Junxin Zhao,Maomao Ma,Xianghui Yan,Dongman Wan,Zheling Zeng,Ping Yu,Deming Gong
The lipase from Bacillus licheniformis NCU CS-5 was immobilized onto β -cyclodextrin (CD) grafted and aminopropyl-functionalized chitosan-coated Fe 3 O 4 magnetic nanocomposites (Fe 3 O 4 -CTS-APTES-GA- β -CD). Fourier transform infrared spectroscopy, thermogravimetry analysis, X-ray diffraction, scanning electron microscopy and transmission electron microscopy showed that not only the functionalized magnetic nanoparticles were synthesized but also the immobilized lipase was successfully produced. The immobilized lipase exhibited higher optimal pH value (10.5) and temperature (60℃) than the free lipase. The pH and thermal stabilities of the immobilized lipase were improved significantly compared to the free lipase. The immobilized lipase remained more than 80% of the relative activity at temperature of 60 ℃ and pH 12.0. The immobilized lipase also remained over 80% of its relative activity after 28 days of storage and 15 cycles of application. The application of the immobilized lipase in esterification of isoamyl acetate and pentyl valerate showed that maximum esterification efficiency was achieved in n -hexane having 68.0% and 89.2% respectively. Therefore, these results indicated that the Fe 3 O 4 -CTS-APTES-GA- β -CD nanoparticles are novel carriers for immobilizing enzyme, and the immobilized lipase can be used as an innovative green approach to the synthesis of fruity flavor esters in food industry.
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 21
·17016-17022
·
IF 3.289
Mengzhen Dai,Ting Huang,Long Chao,Yueming Tan,Chao Chen,Wenhua Meng,Qingji Xie
Learning from nature emerges as one of the most promising ways to develop advanced functional materials and biodevices. Here, inspired by melanin formation, we report the tyrosinase (Tyr)-catalyzed polymerization of L-DOPA (versus L-tyrosine and dopamine) to immobilize enzymes for amperometric biosensing. The enzymatic polymerization is examined by UV-vis spectrophotometry, scanning electron microscopy and electrochemical methods. A poly(L-DOPA) (PD)-Tyr/glassy carbon electrode (GCE) prepared by casting an aqueous mixture of L-DOPA and Tyr on a GCE exhibits a linear cathodic amperometric response to catechol concentration from 0.4 to 57 μM (R2 = 0.997) with a sensitivity of 4.29 mA mM−1 cm−2 and a limit of detection (LOD) of 70 nM (S/N = 3). A PD-glucose oxidase (GOx)-Tyr/Pt electrode prepared by casting an aqueous mixture of L-DOPA, GOx and Tyr on a Pt electrode exhibits a linear anodic amperometric response to glucose concentration from 2 to 5700 μM (R2 = 0.998) with a sensitivity of 78.6 μA mM−1 cm−2 and a LOD of 0.1 μM (S/N = 3). The PD-based enzyme electrode shows better biosensing performance and higher bioactivity of the immobilized Tyr than those based on similarly biosynthesized poly(L-tyrosine) and polydopamine as well as well-established chitosan and Nafion systems, implying that the biosynthesized PD as a melanin-biomimetic material has promising application potential for biomacromolecular immobilization and biosensing.
✓ 已验证
DOI
高影响因子
Environmental Technology & Innovation
·
2026
·
IF 7.3
Mengqi Hao,Shurun Yao,Chuanlong Huang,Lirong Wang,Gang Liu,Zhuo Wei
Engineered biochar gain increasing research interests on persistent organic pollutants remediation due to various degradation pathways. In this study, Fe-loaded biochar immobilized catalase (CAT) composite modified with bacterial extracellular polymeric substances (EPS) (CAT-EPS-FeBC) or glutaraldehyde (GA) were synthesized, and their efficiency and mechanisms on dibutyl phthalate (DBP) removal were evaluated. The characterization results showed the advanced association affinity of EPS with both of biochar and CAT, and the formation of Iron oxide (FeO n ) during biochar pyrolysis which enhanced the association between biochar and EPS through electrostatic attraction and nucleophilic. The total DBP removal efficiencies were in order of: CAT-EPS-FeBC (70.76%) > CAT-GA-FeBC (56.02%) > FeBC (36.60%) > BC (28.45%). EPS modification exhibited higher CAT activity than glutaraldehyde (GA) with degradation enzyme, CAT-EPS-FeBC produced stronger free radicals with •OH generated from FeO n and biochar, and 1 O 2 generated from EPS and CAT. The contribution of electrochemical degradation by biochar composites was insignificant. CAT-EPS-FeBC showed lower ecotoxicity on Chlorella vulgaris than did CAT-GA-FeBC. Overall, the findings in this study demonstrated the high DBP degradation efficiency of Fe loaded biochar immobized CAT with EPS modification through pathways including sorption, CAT catalysis, and free radicals. This study can provide new ideas for the development of biochar-immobilized enzyme and the remediation of persistent organic pollutants.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2026
·Vol. 46
·期 16
·118714
·
IF 6.3
Yuan Liu,Jin Wang,Qing Tao,Xi Zhang,Junxia Wang
In this work, Sr 0.5 Zr 2 (PO 4 ) 3 –high-entropy ( Ln 1/ n )PO 4 monazite dual-phase ceramics with n = 5, 6, and 7 were prepared by microwave-assisted sintering for high-level waste immobilization, where Ln 1/5 = (La, Nd, Sm, Eu, Gd) 1/5 , Ln 1/6 = (La, Nd, Sm, Eu, Gd, Dy) 1/6 , and Ln 1/7 = (La, Nd, Sm, Eu, Gd, Dy, Ho) 1/7 . After 28 days of leaching at pH 4.0, 6.2, and 10.0, the LR Sr and LR Ln-average values for the n = 5 samples were 10 −4 –10 −3 and ∼10 −6 g·m −2 ·d −1 , respectively. Both phases exhibited optimal chemical durability at pH 6.2, and Sr and Ln release mechanisms were pH-dependent. After 28 days of leaching in deionized water, the LR Sr and LR Ln-average values for the n = 5, 6, and 7 samples were 10 −4 –10 −3 and 10 −7 –10 −6 g·m −2 ·d −1 , respectively. Increasing configurational entropy in the high-entropy ( Ln 1/ n )PO 4 monazite phase indirectly reduced Sr release through phase-coupled effects, whereas average Ln release remained nearly unchanged.
✓ 已验证
产品关联
DOI
高影响因子
ACS ES&T Engineering
·
2026
·
IF 6.3
Youyu Li,Min Zou
The low grade, high activity, and high toxicity of chromium in chromium-containing sludge present significant challenges for its immobilization and recycling. To effectively stabilize and recover chromium from chromium-containing sludge, this study presents a novel method referred to as “narrow-particle-size- fraction (Fe, Mg)1–xO solid-solution magnetization”. By prescreening the appropriate particle size fraction of (Fe, Mg)1–xO, this process leverages the thermodynamic and kinetic advantages offered by high temperatures to convert the toxic and highly mobile chromium component into a nontoxic, structurally stable, and strongly magnetic (Fe, Mg)3–xCrxO4 (0 ≤ x ≤ 2) solid-solution during roasting. The (Fe, Mg)3–xCrxO4 phase is then isolated using weak magnetic separation. Taken the chromium-containing sludge with a Cr2O3 content of 5.94 wt % as the raw material, after optimizing the (Fe, Mg)1–xO particle size fraction and roasting conditions, the chromium recovery rate in the magnetic concentrate exceeds 92%, while the grade of Cr2O3 increases to 12.47%. Various analyses confirm that the (Fe, Mg)3–xCrxO4 solid-solution is an effective magnetic carrier for chromium in chromium-containing sludge. This study provides valuable insight for the mineralogical immobilization and resource utilization of chromium-containing solid waste.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）